Restriction map for
pFN21AE2411
(Circular) (Six-base) MAPSORT of: pFN21AE2411.seq Check: 5266 from: 1 to: 6415
pFN21AE2411 6415 bp
With 182 enzymes: SgfI *
May 11, 2012 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2419 2923 3037 4478 5862 6130 1632
Size: 719 68 504 114 1441 1384 268 1917
Fragments arranged by size:
1917 1441 1384 719 504 268 114 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 5314 6291 1203
Size: 4111 977 1327
Fragments arranged by size:
4111 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2376 2825 1184
Size: 1192 449 4774
Fragments arranged by size:
4774 1192 449
AlwNI CAG_nnn'CTG
Cuts at: 2329 4158 5730 2329
Size: 1829 1572 3014
Fragments arranged by size:
3014 1829 1572
ApaLI G'TGCA_C
Cuts at: 4472 5628 4472
Size: 1156 5259
ApoI r'AATT_y
Cuts at: 1493 3457 3513 3708 3724 3950 4102 6211 1493
Size: 1964 56 195 16 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 195 152 56 16
AseI AT'TA_AT
Cuts at: 160 1032 3120 4969 160
Size: 872 2088 1849 1606
Fragments arranged by size:
2088 1849 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2711 2753 3740 1870
Size: 73 768 42 987 4545
Fragments arranged by size:
4545 987 768 73 42
BamHI G'GATC_C
Cuts at: 3475 3745 4112 3475
Size: 270 367 5778
Fragments arranged by size:
5778 367 270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3492 3736 5191 618
Size: 325 217 627 1705 244 1455 1842
Fragments arranged by size:
1842 1705 1455 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2025 3734 1391
Size: 634 1709 4072
Fragments arranged by size:
4072 1709 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3655 928
Size: 971 1756 3688
Fragments arranged by size:
3688 1756 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4307 5517 4307
Size: 1210 5205
BclI T'GATC_A
Cuts at: 1548 2166 1548
Size: 618 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 5026 136
Size: 107 122 71 631 605 530 2824 1525
Fragments arranged by size:
2824 1525 631 605 530 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 3497 4476 5632 1790
Size: 1707 979 1156 2573
Fragments arranged by size:
2573 1707 1156 979
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 2779 4146
Size: 416 53 83 399 24 1251 111 1367
Cuts at: 4146 5104 442
Size: 958 1753
Fragments arranged by size:
1753 1367 1251 958 416 399 111 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2595 3003 5075 1239
Size: 992 364 408 2072 2579
Fragments arranged by size:
2579 2072 992 408 364
Bpu10I CC'TnA_GC
Cuts at: 3132 3411 3132
Size: 279 6136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 4540 5405 5703 2078
Size: 2462 865 298 2790
Fragments arranged by size:
2790 2462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5078 882
Size: 617 3579 2219
Fragments arranged by size:
3579 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2338 3085 4206 493
Size: 711 1134 747 1121 2702
Fragments arranged by size:
2702 1134 1121 747 711
BsaBI GATnn'nnATC
Cuts at: 3332 6409 3332
Size: 3077 3338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4222 4604 5239 275
Size: 53 83 186 936 2689 382 635 1451
Fragments arranged by size:
2689 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 3219 3249 1178
Size: 30 165 30 1356 30 430 30 4344
Fragments arranged by size:
4344 1356 430 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2366 2684 1186
Size: 847 333 318 4917
Fragments arranged by size:
4917 847 333 318
BseYI C'CCAG_C
Cuts at: 2394 2415 2798 2878 5618 6114 2394
Size: 21 383 80 2740 496 2695
Fragments arranged by size:
2740 2695 496 383 80 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2637 1163
Size: 573 901 4941
Fragments arranged by size:
4941 901 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3865 4626 5654 1540
Size: 275 186 1108 756 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 756 275 186
BsiHKAI G_wGCw'C
Cuts at: 3039 4476 4561 5632 3039
Size: 1437 85 1071 3822
Fragments arranged by size:
3822 1437 1071 85
BsiWI C'GTAC_G
Cuts at: 2313 2313
Size: 6415
BsmI GAATG_Cn'
Cuts at: 3926 4019 3926
Size: 93 6322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 3039 3497 3734 4476 4561 5632
Size: 399 235 1014 458 237 742 85 1071
Cuts at: 5632 1391
Size: 2174
Fragments arranged by size:
2174 1071 1014 742 458 399 237 235
85
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3770 844
Size: 2926 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4910 5084 65
Size: 1183 3662 174 1396
Fragments arranged by size:
3662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5059 5246 1700
Size: 110 3249 187 2869
Fragments arranged by size:
3249 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2919 3338 96
Size: 2823 419 3173
Fragments arranged by size:
3173 2823 419
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 3184 4475 5487 3184
Size: 1291 1012 4112
Fragments arranged by size:
4112 1291 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2425 794
Size: 1631 4784
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 3475 3745 4112 4497 4514 5966 6052 6064
Size: 1503 270 367 385 17 1452 86 12
Cuts at: 6064 6410 1972
Size: 346 1977
Fragments arranged by size:
1977 1503 1452 385 367 346 270 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 3471 2016
Size: 1455 4960
BtgI C'CryG_G
Cuts at: 513 1065 2004 2065 513
Size: 552 939 61 4863
Fragments arranged by size:
4863 939 552 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 3931 4725 4745 3931
Size: 794 20 5601
Fragments arranged by size:
5601 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2790 2907
Size: 54 1425 50 275 18 364 596 117
Cuts at: 2907 3862 4751 6380 8
Size: 955 889 1629 43
Fragments arranged by size:
1629 1425 955 889 596 364 275 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2438 2502 4345 1353
Size: 744 341 64 1843 3423
Fragments arranged by size:
3423 1843 744 341 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2080 2774 5002 5376 5522 387
Size: 1064 138 491 694 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 694 491 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2351 2419 2595 2923 3003 3037
Size: 393 599 120 68 176 328 80 34
Cuts at: 3037 4478 5075 5862 6130 1239
Size: 1441 597 787 268 1524
Fragments arranged by size:
1524 1441 787 599 597 393 328 268
176 120 80 68 34
EcoO109I rG'GnC_Cy
Cuts at: 2443 2443
Size: 6415
EcoRI G'AATT_C
Cuts at: 3457 3708 3724 4102 3457
Size: 251 16 378 5770
Fragments arranged by size:
5770 378 251 16
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 2117 2402 2886 3609 5562 1678
Size: 439 285 484 723 1953 2531
Fragments arranged by size:
2531 1953 723 484 439 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 3199 3231 3536
Size: 32 135 32 1187 32 408 32 305
Cuts at: 3536 3568 3687 3719 5056 5088 5130 5162 1373
Size: 32 119 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1337 1187 408 305 135 119 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3205 3511 5529 5713 1305
Size: 1900 306 2018 184 2007
Fragments arranged by size:
2018 2007 1900 306 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 2792 2909 10
Size: 54 1425 343 364 596 117 3516
Fragments arranged by size:
3516 1425 596 364 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 2915 4373 4732 4891 518
Size: 648 1641 108 1458 359 159 2042
Fragments arranged by size:
2042 1641 1458 648 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2152 2356 3628 3831 4508 5656 5901 1606
Size: 546 204 1272 203 677 1148 245 2120
Fragments arranged by size:
2120 1272 1148 677 546 245 204 203
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4924
Fragments arranged by size:
4924 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2806 3773 5318 1132
Size: 97 1577 967 1545 2229
Fragments arranged by size:
2229 1577 1545 967 97
PasI CC'CwG_GG
Cuts at: 1348 3488 1348
Size: 2140 4275
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PfoI T'CCnGG_A
Cuts at: 3617 3617
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PmlI CAC'GTG
Cuts at: 2338 3085 2338
Size: 747 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2443 2443
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 2474 1613
Size: 861 5554
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PspXI vC'TCGA_Gb
Cuts at: 2753 2753
Size: 6415
PstI C_TGCA'G
Cuts at: 830 2355 2766 2935 3767 830
Size: 1525 411 169 832 3478
Fragments arranged by size:
3478 1525 832 411 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 2152 3628 2152
Size: 1476 4939
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SapI GCTCTTCn'nnn_
Cuts at: 2097 2438 2502 2097
Size: 341 64 6010
Fragments arranged by size:
6010 341 64
SbfI CC_TGCA'GG
Cuts at: 2766 2935 3767 2766
Size: 169 832 5414
Fragments arranged by size:
5414 832 169
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SfcI C'TryA_G
Cuts at: 826 1045 2351 2762 2931 3045 3763 4898 5579
Size: 219 1306 411 169 114 718 1135 681
Cuts at: 5579 5770 826
Size: 191 1471
Fragments arranged by size:
1471 1306 1135 718 681 411 219 191
169 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2753 4519 5420 5682 820
Size: 197 926 114 696 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 696 262 197 114
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 2806 3773 1132
Size: 1674 967 3774
Fragments arranged by size:
3774 1674 967
SspI AAT'ATT
Cuts at: 5 52 2534 4339 5
Size: 47 2482 1805 2081
Fragments arranged by size:
2482 2081 1805 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2004 3854 513
Size: 552 939 1850 3074
Fragments arranged by size:
3074 1850 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 2966 3123 3501 4442 4459 4612
Size: 1255 849 537 157 378 941 17 153
Cuts at: 4612 4797 5242 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 941 849 537 445 378 185
157 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2554 2676 2919
Size: 274 80 33 51 1443 577 122 243
Cuts at: 2919 3338 4661 6373 96
Size: 419 1323 1712 138
Fragments arranged by size:
1712 1443 1323 577 419 274 243 138
122 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 6375 6407 3219
Size: 32 3124 32 3227
Fragments arranged by size:
3227 3124 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 3625 2570
Size: 1055 5360
XhoI C'TCGA_G
Cuts at: 1943 2753 1943
Size: 810 5605
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 4544 4544
Size: 6415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI