Restriction map for
pFN21AE2410
(Circular) (Six-base) MAPSORT of: pFN21AE2410.seq Check: 4067 from: 1 to: 5563
pFN21AE2410 5563 bp
With 182 enzymes: SgfI *
May 24, 2012 15:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 4462 5439 1203
Size: 3259 977 1327
Fragments arranged by size:
3259 1327 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 2696 1166
Size: 1530 4033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 3306 4878 3306
Size: 1572 3991
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2872 3098 3250 5359 1493
Size: 1379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2847 2888 1870
Size: 73 85 819 41 4545
Fragments arranged by size:
4545 819 85 73 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2247 2280 2247
Size: 33 5530
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2466 2604 2633 2854 2884
Size: 325 217 627 679 138 29 221 30
Cuts at: 2884 4339 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 679 627 325 221 217 138
30 29
BanII G_rGCy'C
Cuts at: 1391 2029 2203 2882 1391
Size: 638 174 679 4072
Fragments arranged by size:
4072 679 638 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4592
BbvCI CC'TCA_GC
Cuts at: 2181 2181
Size: 5563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3455 4665 2292
Size: 1163 1210 3190
Fragments arranged by size:
3190 1210 1163
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4174 136
Size: 107 122 71 631 605 2502 1525
Fragments arranged by size:
2502 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5558 5558
Size: 5563
BlpI GC'TnA_GC
Cuts at: 2076 2980 2076
Size: 904 4659
Bme1580I G_kGCm'C
Cuts at: 1790 2609 2784 2857 3624 4780 1790
Size: 819 175 73 767 1156 2573
Fragments arranged by size:
2573 1156 819 767 175 73
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3294 4252 442
Size: 416 53 83 399 24 1877 958 1753
Fragments arranged by size:
1877 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4223 1239
Size: 2984 2579
Bpu10I CC'TnA_GC
Cuts at: 2181 2205 2181
Size: 24 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2195 3354 493
Size: 711 991 1159 2702
Fragments arranged by size:
2702 1159 991 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3370 3752 4387 275
Size: 53 83 186 936 1837 382 635 1451
Fragments arranged by size:
1837 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3996 4394 4668 4815 5226 3996
Size: 398 274 147 411 4333
Fragments arranged by size:
4333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 4766 5262 4766
Size: 496 5067
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4990
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 3624 3709 4780 2203
Size: 1421 85 1071 2986
Fragments arranged by size:
2986 1421 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2203 2609 2784 2857 2882 3624
Size: 399 239 174 406 175 73 25 742
Cuts at: 3624 3709 4780 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 239 175 174
85 73 25
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2176 2918 844
Size: 1332 742 3489
Fragments arranged by size:
3489 1332 742
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 2557 4058 4232 65
Size: 1183 822 487 1501 174 1396
Fragments arranged by size:
1501 1396 1183 822 487 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2360 4207 4394 1700
Size: 110 550 1847 187 2869
Fragments arranged by size:
2869 1847 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 2211 3623 4635 2211
Size: 1412 1012 3139
Fragments arranged by size:
3139 1412 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 2779 794
Size: 1679 306 3578
Fragments arranged by size:
3578 1679 306
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstXI CCAn_nnnn'nTGG
Cuts at: 2117 2117
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558
Size: 921 367 385 17 1452 86 12 346
Cuts at: 5558 1972
Size: 1977
Fragments arranged by size:
1977 1452 921 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4072
Fragments arranged by size:
4072 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4824
Fragments arranged by size:
4824 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 3079 3873 3893 2088
Size: 991 794 20 3758
Fragments arranged by size:
3758 991 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DraIII CAC_nnn'GTG
Cuts at: 2473 2473
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4570 809
Size: 507 3254 1802
Fragments arranged by size:
3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2512 3010
Size: 54 1425 50 275 18 289 393 498
Cuts at: 3010 3899 5528 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 498 393 289 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2670 3493 1353
Size: 1143 174 823 3423
Fragments arranged by size:
3423 1143 823 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4150 4524 4670 387
Size: 1064 138 2561 374 146 1280
Fragments arranged by size:
2561 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2201 2201
Size: 5563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3626 4223 5010 5278 1239
Size: 393 1994 597 787 268 1524
Fragments arranged by size:
1994 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2730 2730
Size: 5563
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5563
FspI TGC'GCA
Cuts at: 2480 4069 2480
Size: 1589 3974
HaeII r_GCGC'y
Cuts at: 1678 4710 1678
Size: 3032 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 2821 2853 4204
Size: 32 135 32 1004 32 213 32 1351
Cuts at: 4204 4236 4278 4310 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1351 1004 213 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 2907 3153 669
Size: 988 281 358 611 246 3079
Fragments arranged by size:
3079 988 611 358 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4677 4861 1305
Size: 3372 184 2007
Fragments arranged by size:
3372 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2121 2514 10
Size: 54 1425 343 289 393 3059
Fragments arranged by size:
3059 1425 393 343 289 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2696 3521 3880 4039 518
Size: 648 1414 116 825 359 159 2042
Fragments arranged by size:
2042 1414 825 648 359 159 116
MspA1I CmG'CkG
Cuts at: 1606 2979 3656 4804 5049 1606
Size: 1373 677 1148 245 2120
Fragments arranged by size:
2120 1373 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4072
Fragments arranged by size:
4072 939 552
NdeI CA'TA_TG
Cuts at: 387 2695 387
Size: 2308 3255
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2921 4466 1132
Size: 97 1692 1545 2229
Fragments arranged by size:
2229 1692 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2859 1348
Size: 1511 4052
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2016 2048 2153 2185 3632 3664 5145 5177 2016
Size: 32 105 32 1447 32 1481 32 2402
Fragments arranged by size:
2402 1481 1447 105 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2730 2730
Size: 5563
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PspXI vC'TCGA_Gb
Cuts at: 2847 2847
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2915 830
Size: 2085 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
SacI G_AGCT'C
Cuts at: 2203 2203
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2911 4046 4727 4918 826
Size: 219 1866 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2847 3667 4568 4830 820
Size: 197 926 904 820 901 262 1553
Fragments arranged by size:
1553 926 904 901 820 262 197
SnaBI TAC'GTA
Cuts at: 493 2195 3354 493
Size: 1702 1159 2702
Fragments arranged by size:
2702 1702 1159
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2004 2718 3002 513
Size: 552 939 714 284 3074
Fragments arranged by size:
3074 939 714 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 3590 3607 3760 3945 4390 325
Size: 1255 689 1321 17 153 185 445 1498
Fragments arranged by size:
1498 1321 1255 689 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 3809 5521
Size: 274 80 33 51 1443 711 1121 1712
Cuts at: 5521 96
Size: 138
Fragments arranged by size:
1712 1443 1121 711 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5523 5555 5523
Size: 32 5531
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2798 2899 2798
Size: 101 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2460 2114
Size: 346 5217
XhoI C'TCGA_G
Cuts at: 1943 2847 1943
Size: 904 4659
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 3692 3692
Size: 5563
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI