Restriction map for
pFN21AE2407
(Circular) (Six-base) MAPSORT of: pFN21AE2407.seq Check: 2473 from: 1 to: 9277
pFN21AE2407 9277 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7087 278
Size: 53 83 186 936 5551 2468
Fragments arranged by size:
5551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2179 5494 6620 1937
Size: 242 3315 1126 4594
Fragments arranged by size:
4594 3315 1126 242
Acc65I G'GTAC_C
Cuts at: 6598 6598
Size: 9277
AclI AA'CG_TT
Cuts at: 1563 5506 7404 7777 1563
Size: 3943 1898 373 3063
Fragments arranged by size:
3943 3063 1898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2708 2794 3483 3570 4122 4428 4740
Size: 590 486 86 689 87 552 306 312
Cuts at: 4740 5487 6022 7340 8724 8992 1632
Size: 747 535 1318 1384 268 1917
Fragments arranged by size:
1917 1384 1318 747 689 590 552 535
486 312 306 268 87 86
AflII C'TTAA_G
Cuts at: 820 1017 3224 3932 820
Size: 197 2207 708 6165
Fragments arranged by size:
6165 2207 708 197
AflIII A'CryG_T
Cuts at: 1203 2458 8176 9153 1203
Size: 1255 5718 977 1327
Fragments arranged by size:
5718 1327 1255 977
AgeI A'CCGG_T
Cuts at: 8108 8108
Size: 9277
AhdI GACnn_n'nnGTC
Cuts at: 6171 6515 8006 6171
Size: 344 1491 7442
Fragments arranged by size:
7442 1491 344
AleI CACnn'nnGTG
Cuts at: 1166 3719 1166
Size: 2553 6724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9277
AlwNI CAG_nnn'CTG
Cuts at: 3593 7020 8592 3593
Size: 3427 1572 4278
Fragments arranged by size:
4278 3427 1572
ApaLI G'TGCA_C
Cuts at: 4698 7334 8490 4698
Size: 2636 1156 5485
Fragments arranged by size:
5485 2636 1156
ApoI r'AATT_y
Cuts at: 1493 2313 3906 3994 4172 4727 6586 6812 6964
Size: 820 1593 88 178 555 1859 226 152
Cuts at: 6964 9073 1493
Size: 2109 1697
Fragments arranged by size:
2109 1859 1697 1593 820 555 226 178
152 88
AseI AT'TA_AT
Cuts at: 160 1032 7831 160
Size: 872 6799 1606
Fragments arranged by size:
6799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
AvaI C'yCGr_G
Cuts at: 1870 1943 6602 1870
Size: 73 4659 4545
Fragments arranged by size:
4659 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2177 2210 2177
Size: 33 9244
BamHI G'GATC_C
Cuts at: 6607 6974 6607
Size: 367 8910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6598 8053 618
Size: 325 217 627 4811 1455 1842
Fragments arranged by size:
4811 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4275 6596 1391
Size: 2884 2321 4072
Fragments arranged by size:
4072 2884 2321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4416 5013 5521 5571 5707 928
Size: 971 2517 597 508 50 136 4498
Fragments arranged by size:
4498 2517 971 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 4749 4749
Size: 9277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7468 7502 1903
Size: 34 50 34 5447 34 3678
Fragments arranged by size:
5447 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4483 7169 8379 4483
Size: 2686 1210 5381
Fragments arranged by size:
5381 2686 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9277
BfrBI ATG'CAT
Cuts at: 3314 4796 3314
Size: 1482 7795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7888 136
Size: 107 122 71 631 605 6216 1525
Fragments arranged by size:
6216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 4784 6074 9272 2100
Size: 2684 1290 3198 2105
Fragments arranged by size:
3198 2684 2105 1290
BlpI GC'TnA_GC
Cuts at: 6694 6694
Size: 9277
Bme1580I G_kGCm'C
Cuts at: 1790 4702 7338 8494 1790
Size: 2912 2636 1156 2573
Fragments arranged by size:
2912 2636 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 6517 1318
Size: 5199 4078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 6179 6519
Size: 416 53 83 399 24 2267 2495 340
Cuts at: 6519 7008 7966 442
Size: 489 958 1753
Fragments arranged by size:
2495 2267 1753 958 489 416 399 340
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4709 1056
Size: 3653 5624
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5280 5282 5280
Size: 2 9275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4506 4945 6132 7937 1239
Size: 3267 439 1187 1805 2579
Fragments arranged by size:
3267 2579 1805 1187 439
Bpu10I CC'TnA_GC
Cuts at: 4749 4749
Size: 9277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 4443 5438 5649 5740 7402 8267 8565 2228
Size: 2215 995 211 91 1662 865 298 2940
Fragments arranged by size:
2940 2215 1662 995 865 298 211 91
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2830 5527 7940 882
Size: 617 1331 2697 2413 2219
Fragments arranged by size:
2697 2413 2219 1331 617
BsaAI yAC'GTr
Cuts at: 493 1204 3149 4535 7068 493
Size: 711 1945 1386 2533 2702
Fragments arranged by size:
2702 2533 1945 1386 711
BsaBI GATnn'nnATC
Cuts at: 9271 9271
Size: 9277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7084 7466 8101 275
Size: 53 83 186 936 5551 382 635 1451
Fragments arranged by size:
5551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7710 8108 8382 8529 8940 7710
Size: 398 274 147 411 8047
Fragments arranged by size:
8047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5068 5098 5618 5648 6087
Size: 30 165 30 3665 30 520 30 439
Cuts at: 6087 6117 1178
Size: 30 4338
Fragments arranged by size:
4338 3665 520 439 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3651 5512 5639 5862 6213 1186
Size: 2465 1861 127 223 351 4250
Fragments arranged by size:
4250 2465 1861 351 223 127
BseYI C'CCAG_C
Cuts at: 8480 8976 8480
Size: 496 8781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3532 3557 4568 4839 1163
Size: 573 1796 25 1011 271 5601
Fragments arranged by size:
5601 1796 1011 573 271 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4333 6727 7488 8516 1540
Size: 275 186 2332 2394 761 1028 2301
Fragments arranged by size:
2394 2332 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4275 4702 5813 7338 7423 8494 4275
Size: 427 1111 1525 85 1071 5058
Fragments arranged by size:
5058 1525 1111 1071 427 85
BsmI GAATG_Cn'
Cuts at: 5356 6788 6881 5356
Size: 1432 93 7752
Fragments arranged by size:
7752 1432 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4275 4702 5813 6596 7338 7423 8494
Size: 399 2485 427 1111 783 742 85 1071
Cuts at: 8494 1391
Size: 2174
Fragments arranged by size:
2485 2174 1111 1071 783 742 427 399
85
BspEI T'CCGG_A
Cuts at: 8940 8940
Size: 9277
BspHI T'CATG_A
Cuts at: 6205 7166 8896 6205
Size: 961 1730 6586
Fragments arranged by size:
6586 1730 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 6632 844
Size: 1220 4568 3489
Fragments arranged by size:
4568 3489 1220
BsrBI CCG'CTC
Cuts at: 1394 1823 5185 7164 1394
Size: 429 3362 1979 3507
Fragments arranged by size:
3507 3362 1979 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5157 6045 6327 7772 7946 65
Size: 1183 3909 888 282 1445 174 1396
Fragments arranged by size:
3909 1445 1396 1183 888 282 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6553 7921 8108 1700
Size: 110 4743 1368 187 2869
Fragments arranged by size:
4743 2869 1368 187 110
BsrGI T'GTAC_A
Cuts at: 96 4450 96
Size: 4354 4923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9277
BssSI C'ACGA_G
Cuts at: 2664 7337 8349 2664
Size: 4673 1012 3592
Fragments arranged by size:
4673 3592 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9277
BstBI TT'CG_AA
Cuts at: 6416 8081 6416
Size: 1665 7612
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9277
BstXI CCAn_nnnn'nTGG
Cuts at: 4581 5927 6532 4581
Size: 1346 605 7326
Fragments arranged by size:
7326 1346 605
BstYI r'GATC_y
Cuts at: 1972 2100 3535 4462 4784 6074 6607 6974 7359
Size: 128 1435 927 322 1290 533 367 385
Cuts at: 7359 7376 8828 8914 8926 9272 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1435 1290 927 533 385 367
346 322 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4467 4467
Size: 9277
BtgI C'CryG_G
Cuts at: 513 1065 2049 513
Size: 552 984 7741
Fragments arranged by size:
7741 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5397 5616 6141 487
Size: 683 56 4171 219 525 3623
Fragments arranged by size:
4171 3623 683 525 219 56
BtsI GCAGTG_nn'
Cuts at: 2408 5137 5933 6793 7587 7607 2408
Size: 2729 796 860 794 20 4078
Fragments arranged by size:
4078 2729 860 796 794 20
ClaI AT'CG_AT
Cuts at: 1554 2347 1554
Size: 793 8484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9242
DraI TTT'AAA
Cuts at: 4168 6581 4168
Size: 2413 6864
DraIII CAC_nnn'GTG
Cuts at: 3416 3416
Size: 9277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8284 809
Size: 507 6968 1802
Fragments arranged by size:
6968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5764 6187 6724
Size: 54 1425 50 275 18 3934 423 537
Cuts at: 6724 7613 9242 8
Size: 889 1629 43
Fragments arranged by size:
3934 1629 1425 889 537 423 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6724 1537
Size: 275 4912 4090
Fragments arranged by size:
4912 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2824 3072 3465 4380 4716 5010 5625 5664
Size: 1471 248 393 915 336 294 615 39
Cuts at: 5664 5752 7207 1353
Size: 88 1455 3423
Fragments arranged by size:
3423 1471 1455 915 615 393 336 294
248 88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 7864 8238 8384 387
Size: 1064 138 507 5768 374 146 1280
Fragments arranged by size:
5768 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 4273 4273
Size: 9277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2708 2794 3483 3570 4122 4428
Size: 393 590 486 86 689 87 552 306
Cuts at: 4428 4506 4740 4945 5487 6022 6132 7340 7937
Size: 78 234 205 542 535 110 1208 597
Cuts at: 7937 8724 8992 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1208 787 689 597 590 552 542
535 486 393 306 268 234 205 110
87 86 78
EcoO109I rG'GnC_Cy
Cuts at: 6295 6295
Size: 9277
EcoRI G'AATT_C
Cuts at: 3906 6586 6964 3906
Size: 2680 378 6219
Fragments arranged by size:
6219 2680 378
EcoRV GAT'ATC
Cuts at: 2326 3626 2326
Size: 1300 7977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2544 3128 3947 5078 5222 5315 5808 2544
Size: 584 819 1131 144 93 493 6013
Fragments arranged by size:
6013 1131 819 584 493 144 93
FspI TGC'GCA
Cuts at: 7783 7783
Size: 9277
HaeII r_GCGC'y
Cuts at: 1678 8424 1678
Size: 6746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2340 2372 3561 3587 3593
Size: 32 135 32 768 32 1189 26 6
Cuts at: 3593 3619 4106 4138 5436 5468 6101 6133 6157
Size: 26 487 32 1298 32 633 32 24
Cuts at: 6157 6189 6501 6533 7918 7950 7992 8024 1373
Size: 32 312 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1385 1298 1189 768 633 487 312
135 42 32 32 32 32 32 32
32 32 32 32 26 26 24 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2464 5495 6621 6867 669
Size: 988 281 526 3031 1126 246 3079
Fragments arranged by size:
3079 3031 1126 988 526 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 6503 748
Size: 1930 3825 3522
Fragments arranged by size:
3825 3522 1930
HpaI GTT'AAC
Cuts at: 2464 6867 2464
Size: 4403 4874
KpnI G_GTAC'C
Cuts at: 6602 6602
Size: 9277
MfeI C'AATT_G
Cuts at: 2556 6876 2556
Size: 4320 4957
MluI A'CGCG_T
Cuts at: 9153 9153
Size: 9277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 5234 8391 8575 1305
Size: 3234 695 3157 184 2007
Fragments arranged by size:
3234 3157 2007 695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5766 10
Size: 54 1425 343 3934 3521
Fragments arranged by size:
3934 3521 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2439 3719 5925 7235 7594 7753
Size: 648 949 324 1280 2206 1310 359 159
Cuts at: 7753 518
Size: 2042
Fragments arranged by size:
2206 2042 1310 1280 949 648 359 324
159
MspA1I CmG'CkG
Cuts at: 1606 2011 2051 3899 4552 6261 6693 7370 8518
Size: 405 40 1848 653 1709 432 677 1148
Cuts at: 8518 8763 1606
Size: 245 2120
Fragments arranged by size:
2120 1848 1709 1148 677 653 432 405
245 40
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8725
NdeI CA'TA_TG
Cuts at: 387 2335 4798 387
Size: 1948 2463 4866
Fragments arranged by size:
4866 2463 1948
NheI G'CTAG_C
Cuts at: 1052 4705 1052
Size: 3653 5624
NotI GC'GGCC_GC
Cuts at: 6724 6724
Size: 9277
NruI TCG'CGA
Cuts at: 4329 4329
Size: 9277
NsiI A_TGCA'T
Cuts at: 3316 4798 3316
Size: 1482 7795
NspI r_CATG'y
Cuts at: 1132 1229 2462 4339 4417 6635 8180 1132
Size: 97 1233 1877 78 2218 1545 2229
Fragments arranged by size:
2229 2218 1877 1545 1233 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9277
PciI A'CATG_T
Cuts at: 2458 8176 2458
Size: 5718 3559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2711 1108
Size: 1603 7674
PfoI T'CCnGG_A
Cuts at: 3852 4369 3852
Size: 517 8760
PmeI GTTT'AAAC
Cuts at: 6581 6581
Size: 9277
PmlI CAC'GTG
Cuts at: 4535 4535
Size: 9277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7346 7378 8859 8891 7346
Size: 32 1481 32 7732
Fragments arranged by size:
7732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6295 6295
Size: 9277
PshAI GACnn'nnGTC
Cuts at: 1613 4527 1613
Size: 2914 6363
PsiI TTA'TAA
Cuts at: 5235 6847 5235
Size: 1612 7665
PstI C_TGCA'G
Cuts at: 830 3521 6629 830
Size: 2691 3108 3478
Fragments arranged by size:
3478 3108 2691
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9277
PvuII CAG'CTG
Cuts at: 3899 4552 6261 3899
Size: 653 1709 6915
Fragments arranged by size:
6915 1709 653
SacI G_AGCT'C
Cuts at: 4275 4275
Size: 9277
SacII CC_GC'GG
Cuts at: 2052 2052
Size: 9277
SalI G'TCGA_C
Cuts at: 1936 5493 6619 1936
Size: 3557 1126 4594
Fragments arranged by size:
4594 3557 1126
SapI GCTCTTCn'nnn_
Cuts at: 5752 5752
Size: 9277
SbfI CC_TGCA'GG
Cuts at: 6629 6629
Size: 9277
ScaI AGT'ACT
Cuts at: 5092 7525 9237 5092
Size: 2433 1712 5132
Fragments arranged by size:
5132 2433 1712
SfcI C'TryA_G
Cuts at: 826 1045 3517 3651 5877 6625 7760 8441 8632
Size: 219 2472 134 2226 748 1135 681 191
Cuts at: 8632 826
Size: 1471
Fragments arranged by size:
2472 2226 1471 1135 748 681 219 191
134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9277
SmaI CCC'GGG
Cuts at: 1872 6604 1872
Size: 4732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 3224 3932 4458 5453 5664
Size: 197 926 264 1017 708 526 995 211
Cuts at: 5664 5755 7381 8282 8544 820
Size: 91 1626 901 262 1553
Fragments arranged by size:
1626 1553 1017 995 926 901 708 526
264 262 211 197 91
SnaBI TAC'GTA
Cuts at: 493 7068 493
Size: 6575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9277
SphI G_CATG'C
Cuts at: 1132 6635 1132
Size: 5503 3774
SspI AAT'ATT
Cuts at: 5 52 6085 7201 5
Size: 47 6033 1116 2081
Fragments arranged by size:
6033 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9277
StyI C'CwwG_G
Cuts at: 513 1065 4320 4525 4882 5313 5541 6716 513
Size: 552 3255 205 357 431 228 1175 3074
Fragments arranged by size:
3255 3074 1175 552 431 357 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2812 5830 6538 7304 7321 7474 7659
Size: 1255 1232 3018 708 766 17 153 185
Cuts at: 7659 8104 325
Size: 445 1498
Fragments arranged by size:
3018 1498 1255 1232 766 708 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4450 5090 5111
Size: 274 80 33 51 1443 2473 640 21
Cuts at: 5111 7523 9235 96
Size: 2412 1712 138
Fragments arranged by size:
2473 2412 1712 1443 640 274 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 9237 9269 2374
Size: 32 6831 32 2382
Fragments arranged by size:
6831 2382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9277
XbaI T'CTAG_A
Cuts at: 4637 5510 5525 6613 4637
Size: 873 15 1088 7301
Fragments arranged by size:
7301 1088 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3767 3767
Size: 9277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9277
XmaI C'CCGG_G
Cuts at: 1870 6602 1870
Size: 4732 4545
XmnI GAAnn'nnTTC
Cuts at: 4786 6181 7406 4786
Size: 1395 1225 6657
Fragments arranged by size:
6657 1395 1225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7085 276
Size: 53 83 186 936 5551 2468
Fragments arranged by size:
5551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV PacI PspOMI PspXI PsrI RsrII SanDI SexAI
SfiI SfoI SrfI SwaI