Restriction map for
pFN21AE2403
(Circular) (Six-base) MAPSORT of: pFN21AE2403.seq Check: 7524 from: 1 to: 5341
pFN21AE2403 5341 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2038 2325 2684 1937
Size: 101 287 359 4594
Fragments arranged by size:
4594 359 287 101
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 3404 4788 5056 1632
Size: 561 1211 1384 268 1917
Fragments arranged by size:
1917 1384 1211 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaLI G'TGCA_C
Cuts at: 2194 3398 4554 2194
Size: 1204 1156 2981
Fragments arranged by size:
2981 1204 1156
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2068 2671 3038 2068
Size: 603 367 4371
Fragments arranged by size:
4371 603 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2660 1391
Size: 1269 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 2149 1548
Size: 601 4740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2080 3952 136
Size: 107 122 71 631 605 408 1872 1525
Fragments arranged by size:
1872 1525 631 605 408 122 107 71
BglII A'GATC_T
Cuts at: 2614 5336 2614
Size: 2722 2619
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2198 3402 4558 1790
Size: 408 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 408
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 4504
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 3072 4030
Size: 416 53 83 399 24 759 896 958
Cuts at: 4030 442
Size: 1753
Fragments arranged by size:
1753 958 896 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2332 2432 4001 1239
Size: 1080 13 100 1569 2579
Fragments arranged by size:
2579 1569 1080 100 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 3466 4331 4629 2041
Size: 1425 865 298 2753
Fragments arranged by size:
2753 1425 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 3132 493
Size: 711 1299 629 2702
Fragments arranged by size:
2702 1299 711 629
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2226 2256 1178
Size: 30 165 30 823 30 4263
Fragments arranged by size:
4263 823 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5341
BseYI C'CCAG_C
Cuts at: 2206 4544 5040 2206
Size: 2338 496 2507
Fragments arranged by size:
2507 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 3402 3487 4558 2198
Size: 1204 85 1071 2981
Fragments arranged by size:
2981 1204 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2660 3402 3487 4558 1391
Size: 399 408 462 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 408 399 85
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 2137 3230 4960 2137
Size: 1093 1730 2518
Fragments arranged by size:
2518 1730 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 2696 844
Size: 1542 310 3489
Fragments arranged by size:
3489 1542 310
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 3836 4010 65
Size: 1183 1371 1217 174 1396
Fragments arranged by size:
1396 1371 1217 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2216 794
Size: 1422 3919
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstXI CCAn_nnnn'nTGG
Cuts at: 2099 2297 2099
Size: 198 5143
BstYI r'GATC_y
Cuts at: 1972 2068 2614 2671 3038 3423 3440 4892 4978
Size: 96 546 57 367 385 17 1452 86
Cuts at: 4978 4990 5336 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 546 385 367 346 96 86
57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2558 2857 3651 3671 2558
Size: 299 794 20 4228
Fragments arranged by size:
4228 794 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 4348 809
Size: 507 699 2333 1802
Fragments arranged by size:
2333 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2788 3677 5306
Size: 54 1425 50 275 18 958 889 1629
Cuts at: 5306 8
Size: 43
Fragments arranged by size:
1629 1425 958 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2451 2591 3271 1353
Size: 1098 140 680 3423
Fragments arranged by size:
3423 1098 680 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2061 3928 4302 4448 387
Size: 1064 138 472 1867 374 146 1280
Fragments arranged by size:
1867 1280 1064 472 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2319 2332 2432 3404 4001 4788
Size: 393 561 126 13 100 972 597 787
Cuts at: 4788 5056 1239
Size: 268 1524
Fragments arranged by size:
1524 972 787 597 561 393 268 126
100 13
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 4455 4639 1305
Size: 1005 2145 184 2007
Fragments arranged by size:
2145 2007 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3299 3658 3817 518
Size: 648 2133 359 159 2042
Fragments arranged by size:
2133 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2297 2757 3434 4582 4827 1606
Size: 691 460 677 1148 245 2120
Fragments arranged by size:
2120 1148 691 677 460 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2568 2693 830
Size: 1738 125 3478
Fragments arranged by size:
3478 1738 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2297 2297
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 2117 3589 5301 2117
Size: 1472 1712 2157
Fragments arranged by size:
2157 1712 1472
SfcI C'TryA_G
Cuts at: 826 1045 2564 2689 3824 4505 4696 826
Size: 219 1519 125 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 681 219 191 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2020 3445 4346 4608 820
Size: 197 926 77 1425 901 262 1553
Fragments arranged by size:
1553 1425 926 901 262 197 77
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 1517
Size: 1005 4336
StyI C'CwwG_G
Cuts at: 513 1065 2004 2780 513
Size: 552 939 776 3074
Fragments arranged by size:
3074 939 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 3368 3385 3538 3723 4168 325
Size: 1255 615 1173 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1173 615 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 3587 5299
Size: 274 80 33 51 1443 138 1472 1712
Cuts at: 5299 96
Size: 138
Fragments arranged by size:
1712 1472 1443 274 138 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 5301 5333 2226
Size: 32 3043 32 2234
Fragments arranged by size:
3043 2234 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI