Restriction map for
pFN21AE2400
(Circular) (Six-base) MAPSORT of: pFN21AE2400.seq Check: 2024 from: 1 to: 6802
pFN21AE2400 6802 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2923 4612 278
Size: 53 83 186 936 1387 1689 2468
Fragments arranged by size:
2468 1689 1387 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4145 1937
Size: 2208 4594
Acc65I G'GTAC_C
Cuts at: 4123 4123
Size: 6802
AclI AA'CG_TT
Cuts at: 1563 4929 5302 1563
Size: 3366 373 3063
Fragments arranged by size:
3366 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 3004 3780 3860 4865 6249 6517 1632
Size: 437 935 776 80 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1005 935 776 437 268 80
AfeI AGC'GCT
Cuts at: 2336 2336
Size: 6802
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6605
AflIII A'CryG_T
Cuts at: 1203 3209 5701 6678 1203
Size: 2006 2492 977 1327
Fragments arranged by size:
2492 2006 1327 977
AgeI A'CCGG_T
Cuts at: 5633 5633
Size: 6802
AhdI GACnn_n'nnGTC
Cuts at: 5531 5531
Size: 6802
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3903 1184
Size: 2719 4083
AlwNI CAG_nnn'CTG
Cuts at: 2092 3062 4024 4545 6117 2092
Size: 970 962 521 1572 2777
Fragments arranged by size:
2777 1572 970 962 521
ApaLI G'TGCA_C
Cuts at: 4859 6015 4859
Size: 1156 5646
ApoI r'AATT_y
Cuts at: 1493 2255 2354 2711 3112 3636 4111 4337 4489
Size: 762 99 357 401 524 475 226 152
Cuts at: 4489 6598 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 762 524 475 401 357 226
152 99
AseI AT'TA_AT
Cuts at: 160 1032 3450 5356 160
Size: 872 2418 1906 1606
Fragments arranged by size:
2418 1906 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 4127 1870
Size: 73 645 1539 4545
Fragments arranged by size:
4545 1539 645 73
BamHI G'GATC_C
Cuts at: 4132 4499 4132
Size: 367 6435
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3967 4123 5578 618
Size: 325 217 627 2180 156 1455 1842
Fragments arranged by size:
2180 1842 1455 627 325 217 156
BanII G_rGCy'C
Cuts at: 1391 4121 1391
Size: 2730 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3347 928
Size: 971 1448 4383
Fragments arranged by size:
4383 1448 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 4993 5027 1903
Size: 34 2012 34 1010 34 3678
Fragments arranged by size:
3678 2012 1010 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3517 3922 4694 5904 3517
Size: 405 772 1210 4415
Fragments arranged by size:
4415 1210 772 405
BclI T'GATC_A
Cuts at: 1548 2662 3825 3831 1548
Size: 1114 1163 6 4519
Fragments arranged by size:
4519 1163 1114 6
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5413 136
Size: 107 122 71 631 605 3741 1525
Fragments arranged by size:
3741 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6797 6797
Size: 6802
BlpI GC'TnA_GC
Cuts at: 4219 4219
Size: 6802
Bme1580I G_kGCm'C
Cuts at: 1790 3727 3972 4863 6019 1790
Size: 1937 245 891 1156 2573
Fragments arranged by size:
2573 1937 1156 891 245
BmgBI CAC'GTC
Cuts at: 1318 3212 1318
Size: 1894 4908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2304 4533 5491
Size: 416 53 83 399 24 887 2229 958
Cuts at: 5491 442
Size: 1753
Fragments arranged by size:
2229 1753 958 887 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2114 2969 2981 5462 1239
Size: 845 30 855 12 2481 2579
Fragments arranged by size:
2579 2481 855 845 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 4927 5792 6090 3242
Size: 1685 865 298 3954
Fragments arranged by size:
3954 1685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 3929 5465 882
Size: 617 561 1869 1536 2219
Fragments arranged by size:
2219 1869 1536 617 561
BsaAI yAC'GTr
Cuts at: 493 1204 4593 493
Size: 711 3389 2702
Fragments arranged by size:
3389 2702 711
BsaBI GATnn'nnATC
Cuts at: 3830 6796 3830
Size: 2966 3836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2920 4609 4991 5626
Size: 53 83 186 936 1387 1689 382 635
Cuts at: 5626 275
Size: 1451
Fragments arranged by size:
1689 1451 1387 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5235 5633 5907 6054 6465 5235
Size: 398 274 147 411 5572
Fragments arranged by size:
5572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6577
Fragments arranged by size:
6577 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3239 3390 1186
Size: 2053 151 4598
Fragments arranged by size:
4598 2053 151
BseYI C'CCAG_C
Cuts at: 2331 6005 6501 2331
Size: 3674 496 2632
Fragments arranged by size:
3674 2632 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6229
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4252 5013 6041 1540
Size: 275 186 2251 761 1028 2301
Fragments arranged by size:
2301 2251 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4863 4948 6019 4863
Size: 85 1071 5646
Fragments arranged by size:
5646 1071 85
BsmI GAATG_Cn'
Cuts at: 3874 3996 4313 4406 3874
Size: 122 317 93 6270
Fragments arranged by size:
6270 317 122 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2044 2044
Size: 6802
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3727 3972 4121 4863 4948 6019 1391
Size: 399 1937 245 149 742 85 1071 2174
Fragments arranged by size:
2174 1937 1071 742 399 245 149 85
BspEI T'CCGG_A
Cuts at: 6465 6465
Size: 6802
BspHI T'CATG_A
Cuts at: 3006 3828 3837 4691 6421 3006
Size: 822 9 854 1730 3387
Fragments arranged by size:
3387 1730 854 822 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2801 4157 844
Size: 1957 1356 3489
Fragments arranged by size:
3489 1957 1356
BsrBI CCG'CTC
Cuts at: 1394 1823 4689 1394
Size: 429 2866 3507
Fragments arranged by size:
3507 2866 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 5297 5471 65
Size: 1183 898 3151 174 1396
Fragments arranged by size:
3151 1396 1183 898 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3156 5446 5633 1700
Size: 110 1346 2290 187 2869
Fragments arranged by size:
2869 2290 1346 187 110
BsrGI T'GTAC_A
Cuts at: 96 2263 96
Size: 2167 4635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6802
BssSI C'ACGA_G
Cuts at: 2775 4862 5874 2775
Size: 2087 1012 3703
Fragments arranged by size:
3703 2087 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2793 794
Size: 1999 4803
BstBI TT'CG_AA
Cuts at: 2433 5606 2433
Size: 3173 3629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6802
BstYI r'GATC_y
Cuts at: 1972 3144 3546 4132 4499 4884 4901 6353 6439
Size: 1172 402 586 367 385 17 1452 86
Cuts at: 6439 6451 6797 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1172 586 402 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4070 513
Size: 552 3005 3245
Fragments arranged by size:
3245 3005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6063
Fragments arranged by size:
6063 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 4318 5112 5132 2826
Size: 1492 794 20 4496
Fragments arranged by size:
4496 1492 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6767
DraI TTT'AAA
Cuts at: 2572 4106 2572
Size: 1534 5268
DraIII CAC_nnn'GTG
Cuts at: 2721 3979 2721
Size: 1258 5544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5809 809
Size: 507 4493 1802
Fragments arranged by size:
4493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3474 4249 5138
Size: 54 1425 50 275 18 1644 775 889
Cuts at: 5138 6767 8
Size: 1629 43
Fragments arranged by size:
1644 1629 1425 889 775 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4249 1537
Size: 275 2437 4090
Fragments arranged by size:
4090 2437 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3079 3879 4732 1353
Size: 1726 800 853 3423
Fragments arranged by size:
3423 1726 853 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5389 5763 5909 387
Size: 1064 138 3800 374 146 1280
Fragments arranged by size:
3800 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2084 2114 2969 2981 3004 3780
Size: 393 437 15 30 855 12 23 776
Cuts at: 3780 3860 4865 5462 6249 6517 1239
Size: 80 1005 597 787 268 1524
Fragments arranged by size:
1524 1005 855 787 776 597 437 393
268 80 30 23 15 12
EcoRI G'AATT_C
Cuts at: 4111 4489 4111
Size: 378 6424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3785 3034
Size: 751 6051
FspI TGC'GCA
Cuts at: 5308 5308
Size: 6802
HaeII r_GCGC'y
Cuts at: 1678 2338 5949 1678
Size: 660 3611 2531
Fragments arranged by size:
3611 2531 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2420 2452 3072
Size: 32 135 32 471 32 345 32 620
Cuts at: 3072 3104 3533 3565 5443 5475 5517 5549 1373
Size: 32 429 32 1878 32 42 32 2626
Fragments arranged by size:
2626 1878 620 471 429 345 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 4146 4392 669
Size: 988 281 1153 1055 246 3079
Fragments arranged by size:
3079 1153 1055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6802
HpaI GTT'AAC
Cuts at: 4392 4392
Size: 6802
KpnI G_GTAC'C
Cuts at: 4127 4127
Size: 6802
MfeI C'AATT_G
Cuts at: 2457 2543 4401 2457
Size: 86 1858 4858
Fragments arranged by size:
4858 1858 86
MluI A'CGCG_T
Cuts at: 6678 6678
Size: 6802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 5916 6100 1305
Size: 1258 3353 184 2007
Fragments arranged by size:
3353 2007 1258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3476 10
Size: 54 1425 343 1644 3336
Fragments arranged by size:
3336 1644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3198 3450 3852 4760 5119 5278 518
Size: 648 2032 252 402 908 359 159 2042
Fragments arranged by size:
2042 2032 908 648 402 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 4218 4895 6043 6288 1606
Size: 2612 677 1148 245 2120
Fragments arranged by size:
2612 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3158 3158
Size: 6802
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6250
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6802
NgoMIV G'CCGG_C
Cuts at: 3156 3156
Size: 6802
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6802
NotI GC'GGCC_GC
Cuts at: 4249 4249
Size: 6802
NspI r_CATG'y
Cuts at: 1132 1229 4160 5705 1132
Size: 97 2931 1545 2229
Fragments arranged by size:
2931 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6802
PciI A'CATG_T
Cuts at: 5701 5701
Size: 6802
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 3979 1108
Size: 1437 1434 3931
Fragments arranged by size:
3931 1437 1434
PfoI T'CCnGG_A
Cuts at: 2061 3270 2061
Size: 1209 5593
PmeI GTTT'AAAC
Cuts at: 4106 4106
Size: 6802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4871 4903 6384 6416 4871
Size: 32 1481 32 5257
Fragments arranged by size:
5257 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6802
PsiI TTA'TAA
Cuts at: 4372 4372
Size: 6802
PstI C_TGCA'G
Cuts at: 830 2953 4154 830
Size: 2123 1201 3478
Fragments arranged by size:
3478 2123 1201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6802
SalI G'TCGA_C
Cuts at: 1936 4144 1936
Size: 2208 4594
SapI GCTCTTCn'nnn_
Cuts at: 3879 3879
Size: 6802
SbfI CC_TGCA'GG
Cuts at: 4154 4154
Size: 6802
ScaI AGT'ACT
Cuts at: 3618 5050 6762 3618
Size: 1432 1712 3658
Fragments arranged by size:
3658 1712 1432
SfcI C'TryA_G
Cuts at: 826 1045 2680 2949 4095 4150 5285 5966 6157
Size: 219 1635 269 1146 55 1135 681 191
Cuts at: 6157 826
Size: 1471
Fragments arranged by size:
1635 1471 1146 1135 681 269 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6802
SmaI CCC'GGG
Cuts at: 1872 2590 4129 1872
Size: 718 1539 4545
Fragments arranged by size:
4545 1539 718
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3221 4906 5807 6069 820
Size: 197 926 1278 1685 901 262 1553
Fragments arranged by size:
1685 1553 1278 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4593 493
Size: 4100 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6802
SphI G_CATG'C
Cuts at: 1132 4160 1132
Size: 3028 3774
SspI AAT'ATT
Cuts at: 5 52 3045 4726 5
Size: 47 2993 1681 2081
Fragments arranged by size:
2993 2081 1681 47
StuI AGG'CCT
Cuts at: 1517 2082 1517
Size: 565 6237
StyI C'CwwG_G
Cuts at: 513 1065 2269 2520 4241 513
Size: 552 1204 251 1721 3074
Fragments arranged by size:
3074 1721 1204 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 4829 4846 4999 5184 5629 325
Size: 1255 1527 1722 17 153 185 445 1498
Fragments arranged by size:
1722 1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 2263 3479
Size: 274 80 33 51 1443 120 166 1216
Cuts at: 3479 3616 5048 6760 96
Size: 137 1432 1712 138
Fragments arranged by size:
1712 1443 1432 1216 274 166 138 137
120 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2958 2990 6762 6794 2958
Size: 32 3772 32 2966
Fragments arranged by size:
3772 2966 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6802
XbaI T'CTAG_A
Cuts at: 4138 4138
Size: 6802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6802
XmaI C'CCGG_G
Cuts at: 1870 2588 4127 1870
Size: 718 1539 4545
Fragments arranged by size:
4545 1539 718
XmnI GAAnn'nnTTC
Cuts at: 2220 4931 2220
Size: 2711 4091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2921 4610 276
Size: 53 83 186 936 1387 1689 2468
Fragments arranged by size:
2468 1689 1387 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI