Restriction map for
pFN21AE2399
(Circular) (Six-base) MAPSORT of: pFN21AE2399.seq Check: 9345 from: 1 to: 11560
pFN21AE2399 11560 bp
With 182 enzymes: SgfI *
February 10, 2017 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4077 6892 4077
Size: 2815 8745
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9370 278
Size: 53 83 186 936 7834 2468
Fragments arranged by size:
7834 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 6285 7751 8903 1937
Size: 1683 2665 1466 1152 4594
Fragments arranged by size:
4594 2665 1683 1466 1152
Acc65I G'GTAC_C
Cuts at: 3252 3291 8881 3252
Size: 39 5590 5931
Fragments arranged by size:
5931 5590 39
AclI AA'CG_TT
Cuts at: 1563 9687 10060 1563
Size: 8124 373 3063
Fragments arranged by size:
8124 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2324 3884 7380 8274 8316 8410 9623
Size: 464 228 1560 3496 894 42 94 1213
Cuts at: 9623 11007 11275 1632
Size: 1384 268 1917
Fragments arranged by size:
3496 1917 1560 1384 1213 894 464 268
228 94 42
AfeI AGC'GCT
Cuts at: 2553 2813 3791 7943 2553
Size: 260 978 4152 6170
Fragments arranged by size:
6170 4152 978 260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11363
AflIII A'CryG_T
Cuts at: 1203 7743 10459 11436 1203
Size: 6540 2716 977 1327
Fragments arranged by size:
6540 2716 1327 977
AgeI A'CCGG_T
Cuts at: 10391 10391
Size: 11560
AhdI GACnn_n'nnGTC
Cuts at: 7747 10289 7747
Size: 2542 9018
AleI CACnn'nnGTG
Cuts at: 1166 8456 1166
Size: 7290 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4190 4731 6273 8264 8474 1184
Size: 3006 541 1542 1991 210 4270
Fragments arranged by size:
4270 3006 1991 1542 541 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4771 4803 4771
Size: 32 11528
AlwNI CAG_nnn'CTG
Cuts at: 2209 5366 6866 8353 9303 10875 2209
Size: 3157 1500 1487 950 1572 2894
Fragments arranged by size:
3157 2894 1572 1500 1487 950
ApaI G_GGCC'C
Cuts at: 2186 2286 2330 2598 2964 2990 3064 5436 5759
Size: 100 44 268 366 26 74 2372 323
Cuts at: 5759 5917 6566 7695 8327 2186
Size: 158 649 1129 632 5419
Fragments arranged by size:
5419 2372 1129 649 632 366 323 268
158 100 74 44 26
ApaLI G'TGCA_C
Cuts at: 9617 10773 9617
Size: 1156 10404
ApoI r'AATT_y
Cuts at: 1493 5611 8869 9095 9247 11356 1493
Size: 4118 3258 226 152 2109 1697
Fragments arranged by size:
4118 3258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4806 10114 160
Size: 872 3774 5308 1606
Fragments arranged by size:
5308 3774 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11560
AvaI C'yCGr_G
Cuts at: 1870 1943 2704 2919 2939 3052 3095 5950 8206
Size: 73 761 215 20 113 43 2855 2256
Cuts at: 8206 8234 8885 1870
Size: 28 651 4545
Fragments arranged by size:
4545 2855 2256 761 651 215 113 73
43 28 20
AvrII C'CTAG_G
Cuts at: 2330 2330
Size: 11560
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3236 3269 3485 3518 3236
Size: 33 216 33 11278
Fragments arranged by size:
11278 216 33 33
BamHI G'GATC_C
Cuts at: 5562 5695 7240 8890 9257 5562
Size: 133 1545 1650 367 7865
Fragments arranged by size:
7865 1650 1545 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 2975 3252 3291 4511
Size: 325 217 627 617 571 277 39 1220
Cuts at: 4511 6044 6410 6488 8083 8881 10336 618
Size: 1533 366 78 1595 798 1455 1842
Fragments arranged by size:
1842 1595 1533 1455 1220 798 627 617
571 366 325 277 217 78 39
BanII G_rGCy'C
Cuts at: 1391 2186 2286 2330 2477 2586 2598 2702 2726
Size: 795 100 44 147 109 12 104 24
Cuts at: 2726 2964 2990 3064 3095 3973 4364 5436 5759
Size: 238 26 74 31 878 391 1072 323
Cuts at: 5759 5917 6551 6566 7149 7695 8327 8879 1391
Size: 158 634 15 583 546 632 552 4072
Fragments arranged by size:
4072 1072 878 795 634 632 583 552
546 391 323 238 158 147 109 104
100 74 44 31 26 24 15 12
BbeI G_GCGC'C
Cuts at: 2408 2979 2408
Size: 571 10989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2500 2784 5343 928
Size: 971 601 284 2559 7145
Fragments arranged by size:
7145 2559 971 601 284
BbvCI CC'TCA_GC
Cuts at: 3138 3180 3438 3699 3744 3844 6204 6273 6471
Size: 42 258 261 45 100 2360 69 198
Cuts at: 6471 8369 3138
Size: 1898 6329
Fragments arranged by size:
6329 2360 1898 261 258 198 100 69
45 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3286 3320 5914 5948 9751 9785 1903
Size: 34 1349 34 2594 34 3803 34 3678
Fragments arranged by size:
3803 3678 2594 1349 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3750 6769 9452 10662 3750
Size: 3019 2683 1210 4648
Fragments arranged by size:
4648 3019 2683 1210
BclI T'GATC_A
Cuts at: 1548 4824 7419 1548
Size: 3276 2595 5689
Fragments arranged by size:
5689 3276 2595
BfrBI ATG'CAT
Cuts at: 4216 4528 4708 4216
Size: 312 180 11068
Fragments arranged by size:
11068 312 180
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2498 4183 5763
Size: 107 122 71 631 605 826 1685 1580
Cuts at: 5763 7609 8525 10171 136
Size: 1846 916 1646 1525
Fragments arranged by size:
1846 1685 1646 1580 1525 916 826 631
605 122 107 71
BglII A'GATC_T
Cuts at: 7182 11555 7182
Size: 4373 7187
BlpI GC'TnA_GC
Cuts at: 2696 2805 4309 6480 8977 2696
Size: 109 1504 2171 2497 5279
Fragments arranged by size:
5279 2497 2171 1504 109
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2286 2330 2598 2964 2990 3064 5255
Size: 396 100 44 268 366 26 74 2191
Cuts at: 5255 5436 5759 5917 6566 6690 7695 8327 9621
Size: 181 323 158 649 124 1005 632 1294
Cuts at: 9621 10777 1790
Size: 1156 2573
Fragments arranged by size:
2573 2191 1294 1156 1005 649 632 396
366 323 268 181 158 124 100 74
44 26
BmgBI CAC'GTC
Cuts at: 1318 2904 1318
Size: 1586 9974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3745 4185 4279
Size: 416 53 83 399 24 2328 440 94
Cuts at: 4279 4470 5440 5637 5919 6198 6854 8284 9291
Size: 191 970 197 282 279 656 1430 1007
Cuts at: 9291 10249 442
Size: 958 1753
Fragments arranged by size:
2328 1753 1430 1007 970 958 656 440
416 399 282 279 197 191 94 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11560
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5736 8764 5736
Size: 3028 8532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2752 3521 3847 3898 4002 4512 4551
Size: 1361 152 769 326 51 104 510 39
Cuts at: 4551 4917 5481 5706 7695 8456 10220 1239
Size: 366 564 225 1989 761 1764 2579
Fragments arranged by size:
2579 1989 1764 1361 769 761 564 510
366 326 225 152 104 51 39
Bpu10I CC'TnA_GC
Cuts at: 3138 3180 3438 3699 3744 3844 3893 4135 6204
Size: 42 258 261 45 100 49 242 2069
Cuts at: 6204 6273 6471 8369 8529 3138
Size: 69 198 1898 160 6169
Fragments arranged by size:
6169 2069 1898 261 258 242 198 160
100 69 49 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3801 7016 9685 10550 10848 3801
Size: 3215 2669 865 298 4513
Fragments arranged by size:
4513 3215 2669 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3169 3837 6381 7046 7889 10223 882
Size: 617 1670 668 2544 665 843 2334 2219
Fragments arranged by size:
2544 2334 2219 1670 843 668 665 617
BsaAI yAC'GTr
Cuts at: 493 1204 9351 493
Size: 711 8147 2702
Fragments arranged by size:
8147 2702 711
BsaBI GATnn'nnATC
Cuts at: 5419 7181 11554 5419
Size: 1762 4373 5425
Fragments arranged by size:
5425 4373 1762
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2405 2976 9367 9749
Size: 53 83 186 936 872 571 6391 382
Cuts at: 9749 10384 275
Size: 635 1451
Fragments arranged by size:
6391 1451 936 872 635 571 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4334 4677 7851 9993 10391 10665 10812 11223 4334
Size: 343 3174 2142 398 274 147 411 4671
Fragments arranged by size:
4671 3174 2142 411 398 343 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3519 3549 4378 4408 4549
Size: 30 165 30 2116 30 829 30 141
Cuts at: 4549 4564 4579 4594 4771 4801 8135 8165 8429
Size: 15 15 15 177 30 3334 30 264
Cuts at: 8429 8459 1178
Size: 30 4279
Fragments arranged by size:
4279 3334 2116 829 264 177 165 141
30 30 30 30 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2400 2755 2773 4041 5114 5490 6985
Size: 931 283 355 18 1268 1073 376 1495
Cuts at: 6985 7376 7424 8120 8639 1186
Size: 391 48 696 519 4107
Fragments arranged by size:
4107 1495 1268 1073 931 696 519 391
376 355 283 48 18
BseYI C'CCAG_C
Cuts at: 2209 2440 2598 2865 2909 3380 3462 3561 3573
Size: 231 158 267 44 471 82 99 12
Cuts at: 3573 3612 3726 3765 3783 4299 5044 5382 6068
Size: 39 114 39 18 516 745 338 686
Cuts at: 6068 6223 6228 6234 6261 6420 7837 10763 11259
Size: 155 5 6 27 159 1417 2926 496
Cuts at: 11259 2209
Size: 2510
Fragments arranged by size:
2926 2510 1417 745 686 516 496 471
338 267 231 159 158 155 114 99
82 44 39 39 27 18 12 6
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4500 6384 6587 6809 6914 7514 7526
Size: 573 2764 1884 203 222 105 600 12
Cuts at: 7526 8039 8585 1163
Size: 513 546 4138
Fragments arranged by size:
4138 2764 1884 600 573 546 513 222
203 105 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2139 2427 2470 2502 2850 3048
Size: 275 186 138 288 43 32 348 198
Cuts at: 3048 9010 9771 10799 1540
Size: 5962 761 1028 2301
Fragments arranged by size:
5962 2301 1028 761 348 288 275 198
186 138 43 32
BsiHKAI G_wGCw'C
Cuts at: 2702 3973 3992 5909 6545 7058 7149 9621 9706
Size: 1271 19 1917 636 513 91 2472 85
Cuts at: 9706 10777 2702
Size: 1071 3485
Fragments arranged by size:
3485 2472 1917 1271 1071 636 513 91
85 19
BsmI GAATG_Cn'
Cuts at: 5321 9071 9164 5321
Size: 3750 93 7717
Fragments arranged by size:
7717 3750 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2286 2330 2477 2586 2598 2702
Size: 399 396 100 44 147 109 12 104
Cuts at: 2702 2726 2964 2990 3064 3095 3973 3992 4364
Size: 24 238 26 74 31 878 19 372
Cuts at: 4364 5255 5436 5759 5909 5917 6545 6551 6566
Size: 891 181 323 150 8 628 6 15
Cuts at: 6566 6690 7058 7149 7695 8327 8879 9621 9706
Size: 124 368 91 546 632 552 742 85
Cuts at: 9706 10777 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 891 878 742 632 628 552
546 399 396 372 368 323 238 181
150 147 124 109 104 100 91 85
74 44 31 26 24 19 15 12
8 6
BspEI T'CCGG_A
Cuts at: 4334 11223 4334
Size: 6889 4671
BspHI T'CATG_A
Cuts at: 7107 9449 11179 7107
Size: 2342 1730 7488
Fragments arranged by size:
7488 2342 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 4077 6842 6874 6892 8915 844
Size: 1161 2072 2765 32 18 2023 3489
Fragments arranged by size:
3489 2765 2072 2023 1161 32 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2562 3124 9447 1394
Size: 429 739 562 6323 3507
Fragments arranged by size:
6323 3507 739 562 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 4239 5528 6307 7999 8556 10055
Size: 1183 1000 1991 1289 779 1692 557 1499
Cuts at: 10055 10229 65
Size: 174 1396
Fragments arranged by size:
1991 1692 1499 1396 1289 1183 1000 779
557 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2267 2744 2849 2978 4599 5795 10204
Size: 110 457 477 105 129 1621 1196 4409
Cuts at: 10204 10391 1700
Size: 187 2869
Fragments arranged by size:
4409 2869 1621 1196 477 457 187 129
110 105
BsrGI T'GTAC_A
Cuts at: 96 6168 96
Size: 6072 5488
BssHII G'CGCG_C
Cuts at: 1925 8712 1925
Size: 6787 4773
BssSI C'ACGA_G
Cuts at: 6634 8550 9620 10632 6634
Size: 1916 1070 1012 7562
Fragments arranged by size:
7562 1916 1070 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6217 6430 8552 794
Size: 5423 213 2122 3802
Fragments arranged by size:
5423 3802 2122 213
BstBI TT'CG_AA
Cuts at: 7969 10364 7969
Size: 2395 9165
BstEII G'GTnAC_C
Cuts at: 1184 5774 7214 1184
Size: 4590 1440 5530
Fragments arranged by size:
5530 4590 1440
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2522 3154 2041
Size: 481 632 10447
Fragments arranged by size:
10447 632 481
BstYI r'GATC_y
Cuts at: 1972 5391 5420 5520 5562 5695 7182 7240 7863
Size: 3419 29 100 42 133 1487 58 623
Cuts at: 7863 8890 9257 9642 9659 11111 11197 11209 11555
Size: 1027 367 385 17 1452 86 12 346
Cuts at: 11555 1972
Size: 1977
Fragments arranged by size:
3419 1977 1487 1452 1027 623 385 367
346 133 100 86 58 42 29 17
12
BstZ17I GTA'TAC
Cuts at: 6286 6286
Size: 11560
Bsu36I CC'TnA_GG
Cuts at: 4635 4833 5746 5892 6753 4635
Size: 198 913 146 861 9442
Fragments arranged by size:
9442 913 861 198 146
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2421 2496 2617 2823 2844
Size: 552 939 186 231 75 121 206 21
Cuts at: 2844 3327 3954 4380 4506 4590 4851 4863 5109
Size: 483 627 426 126 84 261 12 246
Cuts at: 5109 5463 6646 7278 513
Size: 354 1183 632 4795
Fragments arranged by size:
4795 1183 939 632 627 552 483 426
354 261 246 231 206 186 126 121
84 75 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2406 5834 487
Size: 683 56 1180 3428 6213
Fragments arranged by size:
6213 3428 1180 683 56
BtsI GCAGTG_nn'
Cuts at: 3014 4164 5269 6315 8568 9076 9870 9890 3014
Size: 1150 1105 1046 2253 508 794 20 4684
Fragments arranged by size:
4684 2253 1150 1105 1046 794 508 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2230 2265 8667 8702 529
Size: 35 1666 35 6402 35 3387
Fragments arranged by size:
6402 3387 1666 35 35 35
DraI TTT'AAA
Cuts at: 7197 8864 7197
Size: 1667 9893
DraIII CAC_nnn'GTG
Cuts at: 4193 4424 6495 4193
Size: 231 2071 9258
Fragments arranged by size:
9258 2071 231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 10567 809
Size: 507 4130 5121 1802
Fragments arranged by size:
5121 4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2136 2424
Size: 54 1425 50 275 18 177 129 288
Cuts at: 2424 2467 2499 2847 3045 4184 4195 4620 4866
Size: 43 32 348 198 1139 11 425 246
Cuts at: 4866 6116 6382 6782 8412 8517 8727 8851 9007
Size: 1250 266 400 1630 105 210 124 156
Cuts at: 9007 9896 11525 8
Size: 889 1629 43
Fragments arranged by size:
1630 1629 1425 1250 1139 889 425 400
348 288 275 266 246 210 198 177
156 129 124 105 54 50 43 43
32 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2136 2424 2467 2499 2847 3045 9007
Size: 275 324 288 43 32 348 198 5962
Cuts at: 9007 1537
Size: 4090
Fragments arranged by size:
5962 4090 348 324 288 275 198 43
32
EarI CTCTTCn'nnn_
Cuts at: 1353 7320 7332 9490 1353
Size: 5967 12 2158 3423
Fragments arranged by size:
5967 3423 2158 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2277 2541 2767 3008 7552 10147
Size: 1064 138 688 264 226 241 4544 2595
Cuts at: 10147 10521 10667 387
Size: 374 146 1280
Fragments arranged by size:
4544 2595 1280 1064 688 374 264 241
226 146 138
EcoICRI GAG'CTC
Cuts at: 2700 3971 7147 2700
Size: 1271 3176 7113
Fragments arranged by size:
7113 3176 1271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2324 2600 2752 3521 3847 3884
Size: 393 464 228 276 152 769 326 37
Cuts at: 3884 3898 4002 4512 4551 4917 5481 5706 7380
Size: 14 104 510 39 366 564 225 1674
Cuts at: 7380 7695 8274 8316 8410 8456 9623 10220 11007
Size: 315 579 42 94 46 1167 597 787
Cuts at: 11007 11275 1239
Size: 268 1524
Fragments arranged by size:
1674 1524 1167 787 769 597 579 564
510 464 393 366 326 315 276 268
228 225 152 104 94 46 42 39
37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3228 4335 5805 5883 8528 3228
Size: 1107 1470 78 2645 6260
Fragments arranged by size:
6260 2645 1470 1107 78
EcoO109I rG'GnC_Cy
Cuts at: 2182 2183 2327 2575 2594 2595 2961 2986 3060
Size: 1 144 248 19 1 366 25 74
Cuts at: 3060 3221 3273 3377 4085 4838 5432 5433 5755
Size: 161 52 104 708 753 594 1 322
Cuts at: 5755 5756 5852 5888 5913 5914 6562 6563 6702
Size: 1 96 36 25 1 648 1 139
Cuts at: 6702 6854 7313 7691 7692 8305 8324 2182
Size: 152 459 378 1 613 19 5418
Fragments arranged by size:
5418 753 708 648 613 594 459 378
366 322 248 161 152 144 139 104
96 74 52 36 25 25 19 19
1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 5611 8869 9247 5611
Size: 3258 378 7924
Fragments arranged by size:
7924 3258 378
EcoRV GAT'ATC
Cuts at: 6919 6919
Size: 11560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7322 7322
Size: 11560
FspI TGC'GCA
Cuts at: 10066 10066
Size: 11560
HaeII r_GCGC'y
Cuts at: 1678 2408 2555 2667 2775 2815 2979 3793 6340
Size: 730 147 112 108 40 164 814 2547
Cuts at: 6340 7945 10707 1678
Size: 1605 2762 2531
Fragments arranged by size:
2762 2547 2531 1605 814 730 164 147
112 108 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2987 3019 3163 3195 4165
Size: 32 135 32 1415 32 144 32 970
Cuts at: 4165 4197 4378 4410 4564 4596 4816 4848 5383
Size: 32 181 32 154 32 220 32 535
Cuts at: 5383 5415 5947 5979 6378 6380 6410 6412 7733
Size: 32 532 32 399 2 30 2 1321
Cuts at: 7733 7765 10201 10233 10275 10307 1373
Size: 32 2436 32 42 32 2626
Fragments arranged by size:
2626 2436 1415 1321 970 535 532 399
220 181 154 144 135 42 32 32
32 32 32 32 32 32 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3395 3621 4929 5206 5451 8904
Size: 988 281 1457 226 1308 277 245 3453
Cuts at: 8904 9150 669
Size: 246 3079
Fragments arranged by size:
3453 3079 1457 1308 988 281 277 246
245 226
HindIII A'AGCT_T
Cuts at: 748 7436 7493 748
Size: 6688 57 4815
Fragments arranged by size:
6688 4815 57
HpaI GTT'AAC
Cuts at: 4929 9150 4929
Size: 4221 7339
KasI G'GCGC_C
Cuts at: 2404 2975 2404
Size: 571 10989
KpnI G_GTAC'C
Cuts at: 3256 3295 8885 3256
Size: 39 5590 5931
Fragments arranged by size:
5931 5590 39
MfeI C'AATT_G
Cuts at: 9159 9159
Size: 11560
MluI A'CGCG_T
Cuts at: 11436 11436
Size: 11560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4182 5964 7189 8196 8760 10674 10858 1305
Size: 2877 1782 1225 1007 564 1914 184 2007
Fragments arranged by size:
2877 2007 1914 1782 1225 1007 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4197 4622 4868 8414 8519
Size: 54 1425 343 2365 425 246 3546 105
Cuts at: 8519 8729 8853 10
Size: 210 124 2717
Fragments arranged by size:
3546 2717 2365 1425 425 343 246 210
124 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4355 4943 5114 8456 8561 9518 9877
Size: 648 3189 588 171 3342 105 957 359
Cuts at: 9877 10036 518
Size: 159 2042
Fragments arranged by size:
3342 3189 2042 957 648 588 359 171
159 105
MspA1I CmG'CkG
Cuts at: 1606 2098 2135 2209 2274 2423 2498 2846 3035
Size: 492 37 74 65 149 75 348 189
Cuts at: 3035 3105 3289 3384 5363 6326 6470 8294 8976
Size: 70 184 95 1979 963 144 1824 682
Cuts at: 8976 9653 10801 11046 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1979 1824 1148 963 682 677 492
348 245 189 184 149 144 95 75
74 70 65 37
NaeI GCC'GGC
Cuts at: 2269 2746 2851 2980 5797 2269
Size: 477 105 129 2817 8032
Fragments arranged by size:
8032 2817 477 129 105
NarI GG'CG_CC
Cuts at: 2405 2976 2405
Size: 571 10989
NcoI C'CATG_G
Cuts at: 513 1065 2004 2823 3327 3954 4380 4506 4590
Size: 552 939 819 504 627 426 126 84
Cuts at: 4590 4851 4863 5109 5463 6646 7278 513
Size: 261 12 246 354 1183 632 4795
Fragments arranged by size:
4795 1183 939 819 632 627 552 504
426 354 261 246 126 84 12
NdeI CA'TA_TG
Cuts at: 387 3187 6133 6667 387
Size: 2800 2946 534 5280
Fragments arranged by size:
5280 2946 2800 534
NgoMIV G'CCGG_C
Cuts at: 2267 2744 2849 2978 5795 2267
Size: 477 105 129 2817 8032
Fragments arranged by size:
8032 2817 477 129 105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11560
NotI GC'GGCC_GC
Cuts at: 2424 2499 3045 9007 2424
Size: 75 546 5962 4977
Fragments arranged by size:
5962 4977 546 75
NsiI A_TGCA'T
Cuts at: 4218 4530 4710 4218
Size: 312 180 11068
Fragments arranged by size:
11068 312 180
NspI r_CATG'y
Cuts at: 1132 1229 4712 5677 6433 6739 7649 7747 8659
Size: 97 3483 965 756 306 910 98 912
Cuts at: 8659 8918 10463 1132
Size: 259 1545 2229
Fragments arranged by size:
3483 2229 1545 965 912 910 756 306
259 98 97
PasI CC'CwG_GG
Cuts at: 1348 3257 4399 5630 5801 6559 7133 8018 8320
Size: 1909 1142 1231 171 758 574 885 302
Cuts at: 8320 1348
Size: 4588
Fragments arranged by size:
4588 1909 1231 1142 885 758 574 302
171
PciI A'CATG_T
Cuts at: 7743 10459 7743
Size: 2716 8844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4193 4424 4553 5630 6125 7703 1108
Size: 3085 231 129 1077 495 1578 4965
Fragments arranged by size:
4965 3085 1578 1077 495 231 129
PfoI T'CCnGG_A
Cuts at: 3979 5565 8172 8256 8385 3979
Size: 1586 2607 84 129 7154
Fragments arranged by size:
7154 2607 1586 129 84
PmeI GTTT'AAAC
Cuts at: 8864 8864
Size: 11560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4771 4803 6785 6817 9629 9661 11142 11174 4771
Size: 32 1982 32 2812 32 1481 32 5157
Fragments arranged by size:
5157 2812 1982 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3221 3273 4838 5852 6854 7313 8305 3221
Size: 52 1565 1014 1002 459 992 6476
Fragments arranged by size:
6476 1565 1014 1002 992 459 52
PshAI GACnn'nnGTC
Cuts at: 1613 3226 5201 1613
Size: 1613 1975 7972
Fragments arranged by size:
7972 1975 1613
PsiI TTA'TAA
Cuts at: 9130 9130
Size: 11560
PspOMI G'GGCC_C
Cuts at: 2182 2282 2326 2594 2960 2986 3060 5432 5755
Size: 100 44 268 366 26 74 2372 323
Cuts at: 5755 5913 6562 7691 8323 2182
Size: 158 649 1129 632 5419
Fragments arranged by size:
5419 2372 1129 649 632 366 323 268
158 100 74 44 26
PstI C_TGCA'G
Cuts at: 830 2226 2559 4159 4882 8912 830
Size: 1396 333 1600 723 4030 3478
Fragments arranged by size:
4030 3478 1600 1396 723 333
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11560
PvuII CAG'CTG
Cuts at: 3035 5363 6326 6470 8294 3035
Size: 2328 963 144 1824 6301
Fragments arranged by size:
6301 2328 1824 963 144
SacI G_AGCT'C
Cuts at: 2702 3973 7149 2702
Size: 1271 3176 7113
Fragments arranged by size:
7113 3176 1271
SacII CC_GC'GG
Cuts at: 2424 2499 2847 2424
Size: 75 348 11137
Fragments arranged by size:
11137 348 75
SalI G'TCGA_C
Cuts at: 1936 3619 8902 1936
Size: 1683 5283 4594
Fragments arranged by size:
5283 4594 1683
SanDI GG'GwC_CC
Cuts at: 3273 4838 3273
Size: 1565 9995
SbfI CC_TGCA'GG
Cuts at: 8912 8912
Size: 11560
ScaI AGT'ACT
Cuts at: 9808 11520 9808
Size: 1712 9848
SfcI C'TryA_G
Cuts at: 826 1045 2222 2555 2950 4155 4878 5782 8908
Size: 219 1177 333 395 1205 723 904 3126
Cuts at: 8908 10043 10724 10915 826
Size: 1135 681 191 1471
Fragments arranged by size:
3126 1471 1205 1177 1135 904 723 681
395 333 219 191
SfoI GGC'GCC
Cuts at: 2406 2977 2406
Size: 571 10989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11560
SmaI CCC'GGG
Cuts at: 1872 2921 2941 3097 8887 1872
Size: 1049 20 156 5790 4545
Fragments arranged by size:
5790 4545 1049 156 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3816 7031 9664 10565 10827 820
Size: 197 926 1873 3215 2633 901 262 1553
Fragments arranged by size:
3215 2633 1873 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 9351 493
Size: 8858 2702
SpeI A'CTAG_T
Cuts at: 152 8681 152
Size: 8529 3031
SphI G_CATG'C
Cuts at: 1132 6739 8918 1132
Size: 5607 2179 3774
Fragments arranged by size:
5607 3774 2179
SspI AAT'ATT
Cuts at: 5 52 7160 9484 5
Size: 47 7108 2324 2081
Fragments arranged by size:
7108 2324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2330 2823 3327 3954 4012 4380
Size: 552 939 326 493 504 627 58 368
Cuts at: 4380 4506 4590 4851 4863 4912 5109 5463 6040
Size: 126 84 261 12 49 197 354 577
Cuts at: 6040 6646 7278 8119 8184 8724 8730 8999 513
Size: 606 632 841 65 540 6 269 3074
Fragments arranged by size:
3074 939 841 632 627 606 577 552
540 504 493 368 354 326 269 261
197 126 84 65 58 49 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4204 4499 4844 4984 5036 5062 5762
Size: 1255 2624 295 345 140 52 26 700
Cuts at: 5762 6053 7202 7746 7787 8031 8082 8490 9587
Size: 291 1149 544 41 244 51 408 1097
Cuts at: 9587 9604 9757 9942 10387 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2624 1498 1255 1149 1097 700 544 445
408 345 295 291 244 185 153 140
52 51 41 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 4287 6168
Size: 274 80 33 51 1443 660 1650 1881
Cuts at: 6168 8836 9806 11518 96
Size: 2668 970 1712 138
Fragments arranged by size:
2668 1881 1712 1650 1443 970 660 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3519 3551 4549 4581 8135 8167 11520 11552 3519
Size: 32 998 32 3554 32 3353 32 3527
Fragments arranged by size:
3554 3527 3353 998 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6392 1316
Size: 5076 6484
XbaI T'CTAG_A
Cuts at: 8896 8896
Size: 11560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2519 4483 5245 5461 5767 6665 2519
Size: 1964 762 216 306 898 7414
Fragments arranged by size:
7414 1964 898 762 306 216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11560
XmaI C'CCGG_G
Cuts at: 1870 2919 2939 3095 8885 1870
Size: 1049 20 156 5790 4545
Fragments arranged by size:
5790 4545 1049 156 20
XmnI GAAnn'nnTTC
Cuts at: 7305 9689 7305
Size: 2384 9176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9368 276
Size: 53 83 186 936 7834 2468
Fragments arranged by size:
7834 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BsiWI BsmBI FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI