Restriction map for
pFN21AE2398
(Circular) (Six-base) MAPSORT of: pFN21AE2398.seq Check: 6125 from: 1 to: 5719
pFN21AE2398 5719 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2775 3529 278
Size: 53 83 186 936 1239 754 2468
Fragments arranged by size:
2468 1239 936 754 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 2538 4550 2538
Size: 2012 3707
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2569 1184
Size: 1385 4334
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaI G_GGCC'C
Cuts at: 2743 2743
Size: 5719
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 1870
Size: 73 1101 4545
Fragments arranged by size:
4545 1101 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2245 2278 2245
Size: 33 5686
BamHI G'GATC_C
Cuts at: 2172 3049 3416 2172
Size: 877 367 4475
Fragments arranged by size:
4475 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3040 4495 618
Size: 325 217 627 1253 1455 1842
Fragments arranged by size:
1842 1455 1253 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2275 2743 3013 3038 1391
Size: 884 468 270 25 4072
Fragments arranged by size:
4072 884 468 270 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2219 928
Size: 971 320 4428
Fragments arranged by size:
4428 971 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 2334 1548
Size: 786 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 2892 4330
Size: 107 122 71 631 605 401 819 1438
Cuts at: 4330 136
Size: 1525
Fragments arranged by size:
1525 1438 819 631 605 401 122 107
71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2743 3780 4936 1790
Size: 953 1037 1156 2573
Fragments arranged by size:
2573 1156 1037 953
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 3450 4408
Size: 416 53 83 399 24 828 1205 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1205 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2326 4379 1239
Size: 959 128 2053 2579
Fragments arranged by size:
2579 2053 959 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 3844 4709 5007 2261
Size: 1583 865 298 2973
Fragments arranged by size:
2973 1583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2322 2772 3526
Size: 53 83 186 936 558 231 450 754
Cuts at: 3526 3908 4543 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 754 635 558 450 382 231
186 83 53
BsaWI w'CCGG_w
Cuts at: 2538 2559 2598 2691 2862 4152 4550 4824 4971
Size: 21 39 93 171 1290 398 274 147
Cuts at: 4971 5382 2538
Size: 411 2875
Fragments arranged by size:
2875 1290 411 398 274 171 147 93
39 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 2450 4922 5418 2450
Size: 2472 496 2751
Fragments arranged by size:
2751 2472 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2364 1163
Size: 573 628 4518
Fragments arranged by size:
4518 628 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2754 2836 3169 3930 4958 1540
Size: 275 186 753 82 333 761 1028 2301
Fragments arranged by size:
2301 1028 761 753 333 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2184 3013 3780 3865 4936 2184
Size: 829 767 85 1071 2967
Fragments arranged by size:
2967 1071 829 767 85
BsmI GAATG_Cn'
Cuts at: 2226 3230 3323 2226
Size: 1004 93 4622
Fragments arranged by size:
4622 1004 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2275 2743 3013 3038 3780 3865
Size: 399 394 91 468 270 25 742 85
Cuts at: 3865 4936 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 394 270 91
85 25
BspEI T'CCGG_A
Cuts at: 2559 2862 5382 2559
Size: 303 2520 2896
Fragments arranged by size:
2896 2520 303
BspHI T'CATG_A
Cuts at: 2331 3608 5338 2331
Size: 1277 1730 2712
Fragments arranged by size:
2712 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2668 3074 844
Size: 1283 541 406 3489
Fragments arranged by size:
3489 1283 541 406
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2538 2835 2891 4363 4550 1700
Size: 110 728 297 56 1472 187 2869
Fragments arranged by size:
2869 1472 728 297 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 2523 96
Size: 2427 3292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 794
Size: 1721 3998
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2172 2239 3049 3416 3801 3818 5270 5356
Size: 200 67 810 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 810 385 367 346 200 86
67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2748 513
Size: 552 939 744 3484
Fragments arranged by size:
3484 939 744 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2177 487
Size: 683 56 951 4029
Fragments arranged by size:
4029 951 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 2400 3023 2400
Size: 623 5096
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2460 2733
Size: 54 1425 50 275 18 336 294 273
Cuts at: 2733 2751 2820 2833 2850 2889 3166 4055 5684
Size: 18 69 13 17 39 277 889 1629
Cuts at: 5684 8
Size: 43
Fragments arranged by size:
1629 1425 889 336 294 277 275 273
69 54 50 43 39 18 18 17
13
EagI C'GGCC_G
Cuts at: 1537 1812 2751 2833 3166 1537
Size: 275 939 82 333 4090
Fragments arranged by size:
4090 939 333 275 82
EarI CTCTTCn'nnn_
Cuts at: 1353 3649 1353
Size: 2296 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3011 3011
Size: 5719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2326 3782 4379 5166 5434 1239
Size: 393 566 128 1456 597 787 268 1524
Fragments arranged by size:
1524 1456 787 597 566 393 268 128
EcoO109I rG'GnC_Cy
Cuts at: 2310 2589 2739 2740 2310
Size: 279 150 1 5289
Fragments arranged by size:
5289 279 150 1
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5719
FspI TGC'GCA
Cuts at: 2015 4225 2015
Size: 2210 3509
HaeII r_GCGC'y
Cuts at: 1678 2133 2515 2995 4866 1678
Size: 455 382 480 1871 2531
Fragments arranged by size:
2531 1871 480 455 382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2224 2250 2256 4360
Size: 32 135 32 646 6 26 6 2104
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2104 646 135 42 32 32 32
32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 2683 3063 3309 669
Size: 988 281 388 357 380 246 3079
Fragments arranged by size:
3079 988 388 380 357 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 4833 5017 1305
Size: 777 2751 184 2007
Fragments arranged by size:
2751 2007 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 2822 2852 10
Size: 54 1425 343 630 360 30 2877
Fragments arranged by size:
2877 1425 630 360 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2336 3677 4036 4195 518
Size: 648 981 189 1341 359 159 2042
Fragments arranged by size:
2042 1341 981 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2553 2617 2750 2832 2998 3135 3812
Size: 413 534 64 133 82 166 137 677
Cuts at: 3812 4960 5205 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 413 245 166 137
133 82 64
NaeI GCC'GGC
Cuts at: 2837 2893 2837
Size: 56 5663
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4228
Fragments arranged by size:
4228 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2835 2891 2835
Size: 56 5663
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 2751 3166 2751
Size: 415 5304
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 2952 1108
Size: 933 911 3875
Fragments arranged by size:
3875 933 911
PfoI T'CCnGG_A
Cuts at: 2175 2175
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2739 2739
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2673 3071 830
Size: 1843 398 3478
Fragments arranged by size:
3478 1843 398
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2553 2617 2998 2553
Size: 64 381 5274
Fragments arranged by size:
5274 381 64
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 5719
SacI G_AGCT'C
Cuts at: 3013 3013
Size: 5719
SacII CC_GC'GG
Cuts at: 2751 2751
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 2673 3071 2673
Size: 398 5321
ScaI AGT'ACT
Cuts at: 2978 3967 5679 2978
Size: 989 1712 3018
Fragments arranged by size:
3018 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 2669 3067 4202 4883 5074 826
Size: 219 1624 398 1135 681 191 1471
Fragments arranged by size:
1624 1471 1135 681 398 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 3823 4724 4986 820
Size: 197 926 333 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 333 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2004 2419 3158 513
Size: 552 939 415 739 3074
Fragments arranged by size:
3074 939 739 552 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2176 2309 2330 2591 3746 3763
Size: 1255 579 17 133 21 261 1155 17
Cuts at: 3763 3916 4101 4546 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1155 579 445 261 185 153
133 21 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2523 2976
Size: 274 80 33 51 1443 276 270 453
Cuts at: 2976 3965 5677 96
Size: 989 1712 138
Fragments arranged by size:
1712 1443 989 453 276 274 270 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 2995 2764
Size: 231 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2773 3527 276
Size: 53 83 186 936 1239 754 2468
Fragments arranged by size:
2468 1239 936 754 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI PsrI SanDI SapI SexAI SfiI
SfoI SrfI SwaI