Restriction map for
pFN21AE2395
(Circular) (Six-base) MAPSORT of: pFN21AE2395.seq Check: 6858 from: 1 to: 8092
pFN21AE2395 8092 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5902 278
Size: 53 83 186 936 4366 2468
Fragments arranged by size:
4366 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 5435 1937
Size: 936 2562 4594
Fragments arranged by size:
4594 2562 936
Acc65I G'GTAC_C
Cuts at: 3911 5413 3911
Size: 1502 6590
AclI AA'CG_TT
Cuts at: 1563 6219 6592 1563
Size: 4656 373 3063
Fragments arranged by size:
4656 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 3087 3927 5202 5259 6155 7539 7807
Size: 639 816 840 1275 57 896 1384 268
Cuts at: 7807 1632
Size: 1917
Fragments arranged by size:
1917 1384 1275 896 840 816 639 268
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7895
AflIII A'CryG_T
Cuts at: 1203 2510 5257 6991 7968 1203
Size: 1307 2747 1734 977 1327
Fragments arranged by size:
2747 1734 1327 1307 977
AgeI A'CCGG_T
Cuts at: 6923 6923
Size: 8092
AhdI GACnn_n'nnGTC
Cuts at: 6821 6821
Size: 8092
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3411 4941 1184
Size: 2227 1530 4335
Fragments arranged by size:
4335 2227 1530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 8060
AlwNI CAG_nnn'CTG
Cuts at: 5835 7407 5835
Size: 1572 6520
ApaLI G'TGCA_C
Cuts at: 6149 7305 6149
Size: 1156 6936
ApoI r'AATT_y
Cuts at: 1493 2496 3840 4337 4384 4678 4786 4951 5317
Size: 1003 1344 497 47 294 108 165 366
Cuts at: 5317 5401 5627 5779 7888 1493
Size: 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 1344 1003 497 366 294 226
165 152 108 84 47
AseI AT'TA_AT
Cuts at: 160 1032 2157 2754 4421 6646 160
Size: 872 1125 597 1667 2225 1606
Fragments arranged by size:
2225 1667 1606 1125 872 597
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
AvaI C'yCGr_G
Cuts at: 1870 1943 5417 1870
Size: 73 3474 4545
Fragments arranged by size:
4545 3474 73
BamHI G'GATC_C
Cuts at: 5422 5789 5422
Size: 367 7725
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3911 5413 6868 618
Size: 325 217 627 2124 1502 1455 1842
Fragments arranged by size:
2124 1842 1502 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5411 1391
Size: 4020 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 3093 3179 928
Size: 971 234 960 86 5841
Fragments arranged by size:
5841 971 960 234 86
BbvCI CC'TCA_GC
Cuts at: 4599 4599
Size: 8092
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6283 6317 1903
Size: 34 50 34 4262 34 3678
Fragments arranged by size:
4262 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5984 7194 4052
Size: 1932 1210 4950
Fragments arranged by size:
4950 1932 1210
BclI T'GATC_A
Cuts at: 1548 4021 1548
Size: 2473 5619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6703 136
Size: 107 122 71 631 605 5031 1525
Fragments arranged by size:
5031 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3475 8087 3475
Size: 4612 3480
BlpI GC'TnA_GC
Cuts at: 5509 5509
Size: 8092
Bme1580I G_kGCm'C
Cuts at: 1790 3658 6153 7309 1790
Size: 1868 2495 1156 2573
Fragments arranged by size:
2573 2495 1868 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 4039 5823
Size: 416 53 83 399 24 1499 1123 1784
Cuts at: 5823 6781 442
Size: 958 1753
Fragments arranged by size:
1784 1753 1499 1123 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4349 6752 1239
Size: 3110 2403 2579
Fragments arranged by size:
3110 2579 2403
Bpu10I CC'TnA_GC
Cuts at: 4599 4914 4599
Size: 315 7777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6217 7082 7380 6217
Size: 865 298 6929
Fragments arranged by size:
6929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2724 6755 882
Size: 617 1225 4031 2219
Fragments arranged by size:
4031 2219 1225 617
BsaAI yAC'GTr
Cuts at: 493 1204 3925 3975 5883 493
Size: 711 2721 50 1908 2702
Fragments arranged by size:
2721 2702 1908 711 50
BsaBI GATnn'nnATC
Cuts at: 2142 3789 8086 2142
Size: 1647 4297 2148
Fragments arranged by size:
4297 2148 1647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5899 6281 6916 275
Size: 53 83 186 936 4366 382 635 1451
Fragments arranged by size:
4366 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6525 6923 7197 7344 7755 6525
Size: 398 274 147 411 6862
Fragments arranged by size:
6862 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2704 2734 3964 3994 1178
Size: 30 165 30 1301 30 1230 30 5276
Fragments arranged by size:
5276 1301 1230 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3212 3215 4196 5022 1186
Size: 2026 3 981 826 4256
Fragments arranged by size:
4256 2026 981 826 3
BseYI C'CCAG_C
Cuts at: 3820 4056 5017 7295 7791 3820
Size: 236 961 2278 496 4121
Fragments arranged by size:
4121 2278 961 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3530 4728 4830 1163
Size: 573 1794 1198 102 4425
Fragments arranged by size:
4425 1794 1198 573 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5542 6303 7331 1540
Size: 275 186 3541 761 1028 2301
Fragments arranged by size:
3541 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4005 4262 6153 6238 7309 4005
Size: 257 1891 85 1071 4788
Fragments arranged by size:
4788 1891 1071 257 85
BsmI GAATG_Cn'
Cuts at: 3225 3954 4071 4534 5603 5696 3225
Size: 729 117 463 1069 93 5621
Fragments arranged by size:
5621 1069 729 463 117 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3658 4005 4262 5411 6153 6238 7309
Size: 399 1868 347 257 1149 742 85 1071
Cuts at: 7309 1391
Size: 2174
Fragments arranged by size:
2174 1868 1149 1071 742 399 347 257
85
BspEI T'CCGG_A
Cuts at: 7755 7755
Size: 8092
BspHI T'CATG_A
Cuts at: 3093 5981 7711 3093
Size: 2888 1730 3474
Fragments arranged by size:
3474 2888 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 5447 844
Size: 1891 2712 3489
Fragments arranged by size:
3489 2712 1891
BsrBI CCG'CTC
Cuts at: 1394 1823 5979 1394
Size: 429 4156 3507
Fragments arranged by size:
4156 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6587 6761 65
Size: 1183 5339 174 1396
Fragments arranged by size:
5339 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6736 6923 1700
Size: 110 4926 187 2869
Fragments arranged by size:
4926 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8092
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8092
BssSI C'ACGA_G
Cuts at: 6152 7164 6152
Size: 1012 7080
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8092
BstBI TT'CG_AA
Cuts at: 3946 6896 3946
Size: 2950 5142
BstEII G'GTnAC_C
Cuts at: 1184 3851 1184
Size: 2667 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 3406 3406
Size: 8092
BstYI r'GATC_y
Cuts at: 1972 2339 2477 3039 3475 5422 5789 6174 6191
Size: 367 138 562 436 1947 367 385 17
Cuts at: 6191 7643 7729 7741 8087 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1947 1452 562 436 385 367 367
346 138 86 17 12
BstZ17I GTA'TAC
Cuts at: 2874 2874
Size: 8092
Bsu36I CC'TnA_GG
Cuts at: 2442 2442
Size: 8092
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7353
Fragments arranged by size:
7353 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 2528 3538 4003 4842 5608 6402 6422 2208
Size: 320 1010 465 839 766 794 20 3878
Fragments arranged by size:
3878 1010 839 794 766 465 320 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2283 2318 2601 2636 4467 4502 529
Size: 35 1719 35 283 35 1831 35 4119
Fragments arranged by size:
4119 1831 1719 283 35 35 35 35
DraI TTT'AAA
Cuts at: 5396 5396
Size: 8092
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3123 7099 809
Size: 507 1807 3976 1802
Fragments arranged by size:
3976 1807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2525 5539 6428
Size: 54 1425 50 275 18 695 3014 889
Cuts at: 6428 8057 8
Size: 1629 43
Fragments arranged by size:
3014 1629 1425 889 695 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5539 1537
Size: 275 3727 4090
Fragments arranged by size:
4090 3727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2641 3864 4248 4485 6022 1353
Size: 833 455 1223 384 237 1537 3423
Fragments arranged by size:
3423 1537 1223 833 455 384 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6679 7053 7199 387
Size: 1064 138 5090 374 146 1280
Fragments arranged by size:
5090 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 3087 3927 4349 5202 5259 6155
Size: 393 639 816 840 422 853 57 896
Cuts at: 6155 6752 7539 7807 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 896 853 840 816 787 639 597
422 393 268 57
EcoNI CCTnn'n_nnAGG
Cuts at: 4080 4080
Size: 8092
EcoRI G'AATT_C
Cuts at: 4337 4786 5401 5779 4337
Size: 449 615 378 6650
Fragments arranged by size:
6650 615 449 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2670 5009 2467
Size: 203 2339 5550
Fragments arranged by size:
5550 2339 203
FspI TGC'GCA
Cuts at: 6598 6598
Size: 8092
HaeII r_GCGC'y
Cuts at: 1678 7239 1678
Size: 5561 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3134 3166 6733 6765 6807
Size: 32 135 32 1562 32 3567 32 42
Cuts at: 6807 6839 1373
Size: 32 2626
Fragments arranged by size:
3567 2626 1562 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5178 5436 5682 669
Size: 988 281 3240 258 246 3079
Fragments arranged by size:
3240 3079 988 281 258 246
HindIII A'AGCT_T
Cuts at: 748 3533 4139 748
Size: 2785 606 4701
Fragments arranged by size:
4701 2785 606
HpaI GTT'AAC
Cuts at: 5682 5682
Size: 8092
KpnI G_GTAC'C
Cuts at: 3915 5417 3915
Size: 1502 6590
MfeI C'AATT_G
Cuts at: 5691 5691
Size: 8092
MluI A'CGCG_T
Cuts at: 7968 7968
Size: 8092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2143 7206 7390 1305
Size: 838 5063 184 2007
Fragments arranged by size:
5063 2007 838 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2527 10
Size: 54 1425 343 695 5575
Fragments arranged by size:
5575 1425 695 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2179 3404 4781 6050 6409 6568 518
Size: 648 1013 1225 1377 1269 359 159 2042
Fragments arranged by size:
2042 1377 1269 1225 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3905 5508 6185 7333 7578 1606
Size: 2299 1603 677 1148 245 2120
Fragments arranged by size:
2299 2120 1603 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7540
NdeI CA'TA_TG
Cuts at: 387 3617 387
Size: 3230 4862
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8092
NotI GC'GGCC_GC
Cuts at: 5539 5539
Size: 8092
NspI r_CATG'y
Cuts at: 1132 1229 2514 3507 5450 6995 1132
Size: 97 1285 993 1943 1545 2229
Fragments arranged by size:
2229 1943 1545 1285 993 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8092
PciI A'CATG_T
Cuts at: 2510 6991 2510
Size: 4481 3611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2597 1108
Size: 1489 6603
PmeI GTTT'AAAC
Cuts at: 5396 5396
Size: 8092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6161 6193 7674 7706 6161
Size: 32 1481 32 6547
Fragments arranged by size:
6547 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8092
PsiI TTA'TAA
Cuts at: 2973 3065 4886 5662 2973
Size: 92 1821 776 5403
Fragments arranged by size:
5403 1821 776 92
PstI C_TGCA'G
Cuts at: 830 2215 2576 2746 4930 5444 830
Size: 1385 361 170 2184 514 3478
Fragments arranged by size:
3478 2184 1385 514 361 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8092
PvuII CAG'CTG
Cuts at: 3905 3905
Size: 8092
SalI G'TCGA_C
Cuts at: 1936 5434 1936
Size: 3498 4594
SbfI CC_TGCA'GG
Cuts at: 5444 5444
Size: 8092
ScaI AGT'ACT
Cuts at: 6340 8052 6340
Size: 1712 6380
SexAI A'CCwGG_T
Cuts at: 2714 2714
Size: 8092
SfcI C'TryA_G
Cuts at: 826 1045 2211 2572 2742 4452 4551 4926 5440
Size: 219 1166 361 170 1710 99 375 514
Cuts at: 5440 6575 7256 7447 826
Size: 1135 681 191 1471
Fragments arranged by size:
1710 1471 1166 1135 681 514 375 361
219 191 170 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8092
SmaI CCC'GGG
Cuts at: 1872 5419 1872
Size: 3547 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6196 7097 7359 820
Size: 197 926 4253 901 262 1553
Fragments arranged by size:
4253 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5883 493
Size: 5390 2702
SpeI A'CTAG_T
Cuts at: 152 3650 152
Size: 3498 4594
SphI G_CATG'C
Cuts at: 1132 5450 1132
Size: 4318 3774
SspI AAT'ATT
Cuts at: 5 52 2735 4807 5101 6016 5
Size: 47 2683 2072 294 915 2081
Fragments arranged by size:
2683 2081 2072 915 294 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8092
StyI C'CwwG_G
Cuts at: 513 1065 3799 4568 4726 5531 513
Size: 552 2734 769 158 805 3074
Fragments arranged by size:
3074 2734 805 769 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3614 4021 6119 6136 6289 6474 6919
Size: 1255 2034 407 2098 17 153 185 445
Cuts at: 6919 325
Size: 1498
Fragments arranged by size:
2098 2034 1498 1255 445 407 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4673 6338 8050
Size: 274 80 33 51 1443 2696 1665 1712
Cuts at: 8050 96
Size: 138
Fragments arranged by size:
2696 1712 1665 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3708 3740 8052 8084 3708
Size: 32 4312 32 3716
Fragments arranged by size:
4312 3716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8092
XbaI T'CTAG_A
Cuts at: 2902 5428 2902
Size: 2526 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 2794
Size: 8092
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8092
XmaI C'CCGG_G
Cuts at: 1870 5417 1870
Size: 3547 4545
XmnI GAAnn'nnTTC
Cuts at: 2256 6221 2256
Size: 3965 4127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5900 276
Size: 53 83 186 936 4366 2468
Fragments arranged by size:
4366 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI DraIII EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI