(Circular) (Six-base) MAPSORT of: pFN21AE2395.seq Check: 6858 from: 1 to: 8092 pFN21AE2395 8092 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5902 278 Size: 53 83 186 936 4366 2468 Fragments arranged by size: 4366 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 5435 1937 Size: 936 2562 4594 Fragments arranged by size: 4594 2562 936 Acc65I G'GTAC_C Cuts at: 3911 5413 3911 Size: 1502 6590 AclI AA'CG_TT Cuts at: 1563 6219 6592 1563 Size: 4656 373 3063 Fragments arranged by size: 4656 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 3087 3927 5202 5259 6155 7539 7807 Size: 639 816 840 1275 57 896 1384 268 Cuts at: 7807 1632 Size: 1917 Fragments arranged by size: 1917 1384 1275 896 840 816 639 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7895 AflIII A'CryG_T Cuts at: 1203 2510 5257 6991 7968 1203 Size: 1307 2747 1734 977 1327 Fragments arranged by size: 2747 1734 1327 1307 977 AgeI A'CCGG_T Cuts at: 6923 6923 Size: 8092 AhdI GACnn_n'nnGTC Cuts at: 6821 6821 Size: 8092 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8092 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3411 4941 1184 Size: 2227 1530 4335 Fragments arranged by size: 4335 2227 1530 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4649 4681 4649 Size: 32 8060 AlwNI CAG_nnn'CTG Cuts at: 5835 7407 5835 Size: 1572 6520 ApaLI G'TGCA_C Cuts at: 6149 7305 6149 Size: 1156 6936 ApoI r'AATT_y Cuts at: 1493 2496 3840 4337 4384 4678 4786 4951 5317 Size: 1003 1344 497 47 294 108 165 366 Cuts at: 5317 5401 5627 5779 7888 1493 Size: 84 226 152 2109 1697 Fragments arranged by size: 2109 1697 1344 1003 497 366 294 226 165 152 108 84 47 AseI AT'TA_AT Cuts at: 160 1032 2157 2754 4421 6646 160 Size: 872 1125 597 1667 2225 1606 Fragments arranged by size: 2225 1667 1606 1125 872 597 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8092 AvaI C'yCGr_G Cuts at: 1870 1943 5417 1870 Size: 73 3474 4545 Fragments arranged by size: 4545 3474 73 BamHI G'GATC_C Cuts at: 5422 5789 5422 Size: 367 7725 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3911 5413 6868 618 Size: 325 217 627 2124 1502 1455 1842 Fragments arranged by size: 2124 1842 1502 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5411 1391 Size: 4020 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2133 3093 3179 928 Size: 971 234 960 86 5841 Fragments arranged by size: 5841 971 960 234 86 BbvCI CC'TCA_GC Cuts at: 4599 4599 Size: 8092 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6283 6317 1903 Size: 34 50 34 4262 34 3678 Fragments arranged by size: 4262 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5984 7194 4052 Size: 1932 1210 4950 Fragments arranged by size: 4950 1932 1210 BclI T'GATC_A Cuts at: 1548 4021 1548 Size: 2473 5619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6703 136 Size: 107 122 71 631 605 5031 1525 Fragments arranged by size: 5031 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3475 8087 3475 Size: 4612 3480 BlpI GC'TnA_GC Cuts at: 5509 5509 Size: 8092 Bme1580I G_kGCm'C Cuts at: 1790 3658 6153 7309 1790 Size: 1868 2495 1156 2573 Fragments arranged by size: 2573 2495 1868 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8092 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 4039 5823 Size: 416 53 83 399 24 1499 1123 1784 Cuts at: 5823 6781 442 Size: 958 1753 Fragments arranged by size: 1784 1753 1499 1123 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8092 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4349 6752 1239 Size: 3110 2403 2579 Fragments arranged by size: 3110 2579 2403 Bpu10I CC'TnA_GC Cuts at: 4599 4914 4599 Size: 315 7777 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6217 7082 7380 6217 Size: 865 298 6929 Fragments arranged by size: 6929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2724 6755 882 Size: 617 1225 4031 2219 Fragments arranged by size: 4031 2219 1225 617 BsaAI yAC'GTr Cuts at: 493 1204 3925 3975 5883 493 Size: 711 2721 50 1908 2702 Fragments arranged by size: 2721 2702 1908 711 50 BsaBI GATnn'nnATC Cuts at: 2142 3789 8086 2142 Size: 1647 4297 2148 Fragments arranged by size: 4297 2148 1647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5899 6281 6916 275 Size: 53 83 186 936 4366 382 635 1451 Fragments arranged by size: 4366 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6525 6923 7197 7344 7755 6525 Size: 398 274 147 411 6862 Fragments arranged by size: 6862 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2704 2734 3964 3994 1178 Size: 30 165 30 1301 30 1230 30 5276 Fragments arranged by size: 5276 1301 1230 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3212 3215 4196 5022 1186 Size: 2026 3 981 826 4256 Fragments arranged by size: 4256 2026 981 826 3 BseYI C'CCAG_C Cuts at: 3820 4056 5017 7295 7791 3820 Size: 236 961 2278 496 4121 Fragments arranged by size: 4121 2278 961 496 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3530 4728 4830 1163 Size: 573 1794 1198 102 4425 Fragments arranged by size: 4425 1794 1198 573 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5542 6303 7331 1540 Size: 275 186 3541 761 1028 2301 Fragments arranged by size: 3541 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4005 4262 6153 6238 7309 4005 Size: 257 1891 85 1071 4788 Fragments arranged by size: 4788 1891 1071 257 85 BsmI GAATG_Cn' Cuts at: 3225 3954 4071 4534 5603 5696 3225 Size: 729 117 463 1069 93 5621 Fragments arranged by size: 5621 1069 729 463 117 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3658 4005 4262 5411 6153 6238 7309 Size: 399 1868 347 257 1149 742 85 1071 Cuts at: 7309 1391 Size: 2174 Fragments arranged by size: 2174 1868 1149 1071 742 399 347 257 85 BspEI T'CCGG_A Cuts at: 7755 7755 Size: 8092 BspHI T'CATG_A Cuts at: 3093 5981 7711 3093 Size: 2888 1730 3474 Fragments arranged by size: 3474 2888 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 5447 844 Size: 1891 2712 3489 Fragments arranged by size: 3489 2712 1891 BsrBI CCG'CTC Cuts at: 1394 1823 5979 1394 Size: 429 4156 3507 Fragments arranged by size: 4156 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6587 6761 65 Size: 1183 5339 174 1396 Fragments arranged by size: 5339 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6736 6923 1700 Size: 110 4926 187 2869 Fragments arranged by size: 4926 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8092 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8092 BssSI C'ACGA_G Cuts at: 6152 7164 6152 Size: 1012 7080 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8092 BstBI TT'CG_AA Cuts at: 3946 6896 3946 Size: 2950 5142 BstEII G'GTnAC_C Cuts at: 1184 3851 1184 Size: 2667 5425 BstXI CCAn_nnnn'nTGG Cuts at: 3406 3406 Size: 8092 BstYI r'GATC_y Cuts at: 1972 2339 2477 3039 3475 5422 5789 6174 6191 Size: 367 138 562 436 1947 367 385 17 Cuts at: 6191 7643 7729 7741 8087 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1947 1452 562 436 385 367 367 346 138 86 17 12 BstZ17I GTA'TAC Cuts at: 2874 2874 Size: 8092 Bsu36I CC'TnA_GG Cuts at: 2442 2442 Size: 8092 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7540 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7353 Fragments arranged by size: 7353 683 56 BtsI GCAGTG_nn' Cuts at: 2208 2528 3538 4003 4842 5608 6402 6422 2208 Size: 320 1010 465 839 766 794 20 3878 Fragments arranged by size: 3878 1010 839 794 766 465 320 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8092 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2283 2318 2601 2636 4467 4502 529 Size: 35 1719 35 283 35 1831 35 4119 Fragments arranged by size: 4119 1831 1719 283 35 35 35 35 DraI TTT'AAA Cuts at: 5396 5396 Size: 8092 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3123 7099 809 Size: 507 1807 3976 1802 Fragments arranged by size: 3976 1807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2525 5539 6428 Size: 54 1425 50 275 18 695 3014 889 Cuts at: 6428 8057 8 Size: 1629 43 Fragments arranged by size: 3014 1629 1425 889 695 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5539 1537 Size: 275 3727 4090 Fragments arranged by size: 4090 3727 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2641 3864 4248 4485 6022 1353 Size: 833 455 1223 384 237 1537 3423 Fragments arranged by size: 3423 1537 1223 833 455 384 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6679 7053 7199 387 Size: 1064 138 5090 374 146 1280 Fragments arranged by size: 5090 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 3087 3927 4349 5202 5259 6155 Size: 393 639 816 840 422 853 57 896 Cuts at: 6155 6752 7539 7807 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 896 853 840 816 787 639 597 422 393 268 57 EcoNI CCTnn'n_nnAGG Cuts at: 4080 4080 Size: 8092 EcoRI G'AATT_C Cuts at: 4337 4786 5401 5779 4337 Size: 449 615 378 6650 Fragments arranged by size: 6650 615 449 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2670 5009 2467 Size: 203 2339 5550 Fragments arranged by size: 5550 2339 203 FspI TGC'GCA Cuts at: 6598 6598 Size: 8092 HaeII r_GCGC'y Cuts at: 1678 7239 1678 Size: 5561 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3134 3166 6733 6765 6807 Size: 32 135 32 1562 32 3567 32 42 Cuts at: 6807 6839 1373 Size: 32 2626 Fragments arranged by size: 3567 2626 1562 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5178 5436 5682 669 Size: 988 281 3240 258 246 3079 Fragments arranged by size: 3240 3079 988 281 258 246 HindIII A'AGCT_T Cuts at: 748 3533 4139 748 Size: 2785 606 4701 Fragments arranged by size: 4701 2785 606 HpaI GTT'AAC Cuts at: 5682 5682 Size: 8092 KpnI G_GTAC'C Cuts at: 3915 5417 3915 Size: 1502 6590 MfeI C'AATT_G Cuts at: 5691 5691 Size: 8092 MluI A'CGCG_T Cuts at: 7968 7968 Size: 8092 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2143 7206 7390 1305 Size: 838 5063 184 2007 Fragments arranged by size: 5063 2007 838 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2527 10 Size: 54 1425 343 695 5575 Fragments arranged by size: 5575 1425 695 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2179 3404 4781 6050 6409 6568 518 Size: 648 1013 1225 1377 1269 359 159 2042 Fragments arranged by size: 2042 1377 1269 1225 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3905 5508 6185 7333 7578 1606 Size: 2299 1603 677 1148 245 2120 Fragments arranged by size: 2299 2120 1603 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7540 NdeI CA'TA_TG Cuts at: 387 3617 387 Size: 3230 4862 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8092 NotI GC'GGCC_GC Cuts at: 5539 5539 Size: 8092 NspI r_CATG'y Cuts at: 1132 1229 2514 3507 5450 6995 1132 Size: 97 1285 993 1943 1545 2229 Fragments arranged by size: 2229 1943 1545 1285 993 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8092 PciI A'CATG_T Cuts at: 2510 6991 2510 Size: 4481 3611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2597 1108 Size: 1489 6603 PmeI GTTT'AAAC Cuts at: 5396 5396 Size: 8092 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6161 6193 7674 7706 6161 Size: 32 1481 32 6547 Fragments arranged by size: 6547 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8092 PsiI TTA'TAA Cuts at: 2973 3065 4886 5662 2973 Size: 92 1821 776 5403 Fragments arranged by size: 5403 1821 776 92 PstI C_TGCA'G Cuts at: 830 2215 2576 2746 4930 5444 830 Size: 1385 361 170 2184 514 3478 Fragments arranged by size: 3478 2184 1385 514 361 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8092 PvuII CAG'CTG Cuts at: 3905 3905 Size: 8092 SalI G'TCGA_C Cuts at: 1936 5434 1936 Size: 3498 4594 SbfI CC_TGCA'GG Cuts at: 5444 5444 Size: 8092 ScaI AGT'ACT Cuts at: 6340 8052 6340 Size: 1712 6380 SexAI A'CCwGG_T Cuts at: 2714 2714 Size: 8092 SfcI C'TryA_G Cuts at: 826 1045 2211 2572 2742 4452 4551 4926 5440 Size: 219 1166 361 170 1710 99 375 514 Cuts at: 5440 6575 7256 7447 826 Size: 1135 681 191 1471 Fragments arranged by size: 1710 1471 1166 1135 681 514 375 361 219 191 170 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8092 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8092 SmaI CCC'GGG Cuts at: 1872 5419 1872 Size: 3547 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6196 7097 7359 820 Size: 197 926 4253 901 262 1553 Fragments arranged by size: 4253 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5883 493 Size: 5390 2702 SpeI A'CTAG_T Cuts at: 152 3650 152 Size: 3498 4594 SphI G_CATG'C Cuts at: 1132 5450 1132 Size: 4318 3774 SspI AAT'ATT Cuts at: 5 52 2735 4807 5101 6016 5 Size: 47 2683 2072 294 915 2081 Fragments arranged by size: 2683 2081 2072 915 294 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8092 StyI C'CwwG_G Cuts at: 513 1065 3799 4568 4726 5531 513 Size: 552 2734 769 158 805 3074 Fragments arranged by size: 3074 2734 805 769 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3614 4021 6119 6136 6289 6474 6919 Size: 1255 2034 407 2098 17 153 185 445 Cuts at: 6919 325 Size: 1498 Fragments arranged by size: 2098 2034 1498 1255 445 407 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4673 6338 8050 Size: 274 80 33 51 1443 2696 1665 1712 Cuts at: 8050 96 Size: 138 Fragments arranged by size: 2696 1712 1665 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3708 3740 8052 8084 3708 Size: 32 4312 32 3716 Fragments arranged by size: 4312 3716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8092 XbaI T'CTAG_A Cuts at: 2902 5428 2902 Size: 2526 5566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 2794 Size: 8092 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8092 XmaI C'CCGG_G Cuts at: 1870 5417 1870 Size: 3547 4545 XmnI GAAnn'nnTTC Cuts at: 2256 6221 2256 Size: 3965 4127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5900 276 Size: 53 83 186 936 4366 2468 Fragments arranged by size: 4366 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI