Restriction map for
pFN21AE2393
(Circular) (Six-base) MAPSORT of: pFN21AE2393.seq Check: 2910 from: 1 to: 6199
pFN21AE2393 6199 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 3542 1937
Size: 454 1151 4594
Fragments arranged by size:
4594 1151 454
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4262 5646 5914 1632
Size: 2630 1384 268 1917
Fragments arranged by size:
2630 1917 1384 268
AfeI AGC'GCT
Cuts at: 3008 3008
Size: 6199
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 5098 6075 1203
Size: 3895 977 1327
Fragments arranged by size:
3895 1327 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 2912 1166
Size: 1746 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 2828 1184
Size: 1170 474 4555
Fragments arranged by size:
4555 1170 474
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 6167
AlwNI CAG_nnn'CTG
Cuts at: 2946 3470 3942 5514 2946
Size: 524 472 1572 3631
Fragments arranged by size:
3631 1572 524 472
ApaI G_GGCC'C
Cuts at: 3480 3480
Size: 6199
ApaLI G'TGCA_C
Cuts at: 2960 4256 5412 2960
Size: 1296 1156 3747
Fragments arranged by size:
3747 1296 1156
ApoI r'AATT_y
Cuts at: 1493 3508 3734 3886 5995 1493
Size: 2015 226 152 2109 1697
Fragments arranged by size:
2109 2015 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 3524 1870
Size: 73 1170 411 4545
Fragments arranged by size:
4545 1170 411 73
BamHI G'GATC_C
Cuts at: 2316 3529 3896 2316
Size: 1213 367 4619
Fragments arranged by size:
4619 1213 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 3520 4975 618
Size: 325 217 627 1205 528 1455 1842
Fragments arranged by size:
1842 1455 1205 627 528 325 217
BanII G_rGCy'C
Cuts at: 1391 3435 3480 3518 1391
Size: 2044 45 38 4072
Fragments arranged by size:
4072 2044 45 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2755 3339 928
Size: 971 856 584 3788
Fragments arranged by size:
3788 971 856 584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4390 4424 1903
Size: 34 2453 34 3678
Fragments arranged by size:
3678 2453 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2964 3425 3480 4260 5416 1790
Size: 1174 461 55 780 1156 2573
Fragments arranged by size:
2573 1174 1156 780 461 55
BmgBI CAC'GTC
Cuts at: 1318 2668 1318
Size: 1350 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2589 3930 4888
Size: 416 53 83 399 24 1172 1341 958
Cuts at: 4888 442
Size: 1753
Fragments arranged by size:
1753 1341 1172 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3186 4859 1239
Size: 1947 1673 2579
Fragments arranged by size:
2579 1947 1673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 4324 5189 5487 2495
Size: 1829 865 298 3207
Fragments arranged by size:
3207 1829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4862 882
Size: 617 3363 2219
Fragments arranged by size:
3363 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 3364 3990 493
Size: 711 1458 702 626 2702
Fragments arranged by size:
2702 1458 711 702 626
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2564 4006 4388 5023
Size: 53 83 186 936 1031 1442 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1451 1442 1031 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2409 4632 5030 5304 5451 5862 2409
Size: 2223 398 274 147 411 2746
Fragments arranged by size:
2746 2223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 1186
Size: 957 5242
BseYI C'CCAG_C
Cuts at: 2140 2193 2654 2975 3089 3309 3489 5402 5898
Size: 53 461 321 114 220 180 1913 496
Cuts at: 5898 2140
Size: 2441
Fragments arranged by size:
2441 1913 496 461 321 220 180 114
53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2488 3002 3296 1163
Size: 573 752 514 294 4066
Fragments arranged by size:
4066 752 573 514 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 2955 2964 4260 4345 5416 2739
Size: 216 9 1296 85 1071 3522
Fragments arranged by size:
3522 1296 1071 216 85 9
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2739 2955 2964 3425 3435 3480 3518
Size: 399 949 216 9 461 10 45 38
Cuts at: 3518 4260 4345 5416 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 949 742 461 399 216 85
45 38 10 9
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 2226 2559 3042 4088 5818 2226
Size: 333 483 1046 1730 2607
Fragments arranged by size:
2607 1730 1046 483 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2778 3554 844
Size: 1934 776 3489
Fragments arranged by size:
3489 1934 776
BsrBI CCG'CTC
Cuts at: 1394 1823 2520 2970 4086 1394
Size: 429 697 450 1116 3507
Fragments arranged by size:
3507 1116 697 450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4694 4868 65
Size: 1183 3446 174 1396
Fragments arranged by size:
3446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3206 3381 4843 5030 1700
Size: 110 1396 175 1462 187 2869
Fragments arranged by size:
2869 1462 1396 187 175 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 2011 2013 1925
Size: 86 2 6111
Fragments arranged by size:
6111 86 2
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3025 3099 794
Size: 2231 74 3894
Fragments arranged by size:
3894 2231 74
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 2920 1184
Size: 1736 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 2749 2917 2662
Size: 87 168 5944
Fragments arranged by size:
5944 168 87
BstYI r'GATC_y
Cuts at: 1972 2316 3529 3896 4281 4298 5750 5836 5848
Size: 344 1213 367 385 17 1452 86 12
Cuts at: 5848 6194 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1213 385 367 346 344 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2312 2312
Size: 6199
BtgI C'CryG_G
Cuts at: 513 1065 2910 2988 513
Size: 552 1845 78 3724
Fragments arranged by size:
3724 1845 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3289 487
Size: 683 56 2063 3397
Fragments arranged by size:
3397 2063 683 56
BtsI GCAGTG_nn'
Cuts at: 3715 4509 4529 3715
Size: 794 20 5385
Fragments arranged by size:
5385 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3341 5206 809
Size: 507 2025 1865 1802
Fragments arranged by size:
2025 1865 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2751 3072
Size: 54 1425 50 275 18 243 678 321
Cuts at: 3072 3646 4535 6164 8
Size: 574 889 1629 43
Fragments arranged by size:
1629 1425 889 678 574 321 275 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3044 3440 3455 4129 1353
Size: 1691 396 15 674 3423
Fragments arranged by size:
3423 1691 674 396 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 4786 5160 5306 387
Size: 1064 138 537 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 537 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3186 4262 4859 5646 5914 1239
Size: 393 1554 1076 597 787 268 1524
Fragments arranged by size:
1554 1524 1076 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3256 3256
Size: 6199
EcoO109I rG'GnC_Cy
Cuts at: 2373 3476 3477 2373
Size: 1103 1 5095
Fragments arranged by size:
5095 1103 1
EcoRI G'AATT_C
Cuts at: 3508 3886 3508
Size: 378 5821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 6199
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 2460 3010 3052 5346 1678
Size: 782 550 42 2294 2531
Fragments arranged by size:
2531 2294 782 550 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 4840 4872 4914
Size: 32 135 32 636 32 2600 32 42
Cuts at: 4914 4946 1373
Size: 32 2626
Fragments arranged by size:
2626 2600 636 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 2802 3168 3289 5313 5497 1305
Size: 1134 363 366 121 2024 184 2007
Fragments arranged by size:
2024 2007 1134 366 363 184 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2753 10
Size: 54 1425 343 243 678 3456
Fragments arranged by size:
3456 1425 678 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 2912 3407 4157 4516 4675 518
Size: 648 1644 102 495 750 359 159 2042
Fragments arranged by size:
2042 1644 750 648 495 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 3615 4292 5440 5685 1606
Size: 2009 677 1148 245 2120
Fragments arranged by size:
2120 2009 1148 677 245
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 6199
NcoI C'CATG_G
Cuts at: 513 1065 2910 2988 513
Size: 552 1845 78 3724
Fragments arranged by size:
3724 1845 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 3557 5102 1132
Size: 97 2328 1545 2229
Fragments arranged by size:
2328 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2624 2997 3231 1348
Size: 1276 373 234 4316
Fragments arranged by size:
4316 1276 373 234
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2241 2989 1108
Size: 1133 748 4318
Fragments arranged by size:
4318 1133 748
PfoI T'CCnGG_A
Cuts at: 2522 2522
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 4268 4300 5781 5813 3167
Size: 32 1069 32 1481 32 3553
Fragments arranged by size:
3553 1481 1069 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2373 2373
Size: 6199
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 3476 3476
Size: 6199
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2888 2920 3149 3181 2888
Size: 32 229 32 5906
Fragments arranged by size:
5906 229 32 32
PstI C_TGCA'G
Cuts at: 830 2181 3190 3551 830
Size: 1351 1009 361 3478
Fragments arranged by size:
3478 1351 1009 361
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SapI GCTCTTCn'nnn_
Cuts at: 3440 3440
Size: 6199
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 2480 4447 6159 2480
Size: 1967 1712 2520
Fragments arranged by size:
2520 1967 1712
SfcI C'TryA_G
Cuts at: 826 1045 2177 3186 3547 4682 5363 5554 826
Size: 219 1132 1009 361 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 1009 681 361 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 4303 5204 5466 820
Size: 197 926 567 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 2910 2988 3638 513
Size: 552 1845 78 650 3074
Fragments arranged by size:
3074 1845 650 552 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 2996 3385 4226 4243 4396 4581
Size: 1255 1298 118 389 841 17 153 185
Cuts at: 4581 5026 325
Size: 445 1498
Fragments arranged by size:
1498 1298 1255 841 445 389 185 153
118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 3165 4445
Size: 274 80 33 51 1443 501 687 1280
Cuts at: 4445 6157 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1280 687 501 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 1316
Size: 1236 4963
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI