Restriction map for
pFN21AE2389
(Circular) (Six-base) MAPSORT of: pFN21AE2389.seq Check: 9462 from: 1 to: 6778
pFN21AE2389 6778 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4588 278
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3876 4121 1937
Size: 1939 245 4594
Fragments arranged by size:
4594 1939 245
Acc65I G'GTAC_C
Cuts at: 4099 4099
Size: 6778
AclI AA'CG_TT
Cuts at: 1563 4905 5278 1563
Size: 3342 373 3063
Fragments arranged by size:
3342 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 4841 6225 6493 1632
Size: 402 2807 1384 268 1917
Fragments arranged by size:
2807 1917 1384 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6581
AflIII A'CryG_T
Cuts at: 1203 2511 3667 3797 3830 5677 6654 1203
Size: 1308 1156 130 33 1847 977 1327
Fragments arranged by size:
1847 1327 1308 1156 977 130 33
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 5507 5507
Size: 6778
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6778
AlwNI CAG_nnn'CTG
Cuts at: 4521 6093 4521
Size: 1572 5206
ApaLI G'TGCA_C
Cuts at: 4835 5991 4835
Size: 1156 5622
ApoI r'AATT_y
Cuts at: 1493 2233 2404 2442 3200 3230 3602 3634 3756
Size: 740 171 38 758 30 372 32 122
Cuts at: 3756 4087 4313 4465 6574 1493
Size: 331 226 152 2109 1697
Fragments arranged by size:
2109 1697 758 740 372 331 226 171
152 122 38 32 30
AseI AT'TA_AT
Cuts at: 160 1032 2436 3932 5332 160
Size: 872 1404 1496 1400 1606
Fragments arranged by size:
1606 1496 1404 1400 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 4103 1870
Size: 73 2160 4545
Fragments arranged by size:
4545 2160 73
BamHI G'GATC_C
Cuts at: 2267 4108 4475 2267
Size: 1841 367 4570
Fragments arranged by size:
4570 1841 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3659 4099 5554 618
Size: 325 217 627 1872 440 1455 1842
Fragments arranged by size:
1872 1842 1455 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 3784 4097 1391
Size: 2393 313 4072
Fragments arranged by size:
4072 2393 313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3073 3882 928
Size: 971 1174 809 3824
Fragments arranged by size:
3824 1174 971 809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4969 5003 1903
Size: 34 3032 34 3678
Fragments arranged by size:
3678 3032 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4670 5880 4670
Size: 1210 5568
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6778
BfrBI ATG'CAT
Cuts at: 3977 3977
Size: 6778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5389 136
Size: 107 122 71 631 605 3717 1525
Fragments arranged by size:
3717 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2725 6773 2725
Size: 4048 2730
BlpI GC'TnA_GC
Cuts at: 4195 4195
Size: 6778
Bme1580I G_kGCm'C
Cuts at: 1790 4839 5995 1790
Size: 3049 1156 2573
Fragments arranged by size:
3049 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3728 4509 5467
Size: 416 53 83 399 24 2311 781 958
Cuts at: 5467 442
Size: 1753
Fragments arranged by size:
2311 1753 958 781 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 6778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3524 5438 1239
Size: 2285 1914 2579
Fragments arranged by size:
2579 2285 1914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4903 5768 6066 4903
Size: 865 298 5615
Fragments arranged by size:
5615 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3542 5441 882
Size: 617 2043 1899 2219
Fragments arranged by size:
2219 2043 1899 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3091 3833 4569 493
Size: 711 1503 384 742 736 2702
Fragments arranged by size:
2702 1503 742 736 711 384
BsaBI GATnn'nnATC
Cuts at: 6772 6772
Size: 6778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4585 4967 5602 275
Size: 53 83 186 936 3052 382 635 1451
Fragments arranged by size:
3052 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5211 5609 5883 6030 6441 5211
Size: 398 274 147 411 5548
Fragments arranged by size:
5548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6553
Fragments arranged by size:
6553 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6778
BseYI C'CCAG_C
Cuts at: 4052 5981 6477 4052
Size: 1929 496 4353
Fragments arranged by size:
4353 1929 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3633 1163
Size: 573 1897 4308
Fragments arranged by size:
4308 1897 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4228 4989 6017 1540
Size: 275 186 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3748 4839 4924 5995 3748
Size: 1091 85 1071 4531
Fragments arranged by size:
4531 1091 1071 85
BsmI GAATG_Cn'
Cuts at: 2647 3578 4289 4382 2647
Size: 931 711 93 5043
Fragments arranged by size:
5043 931 711 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3748 3784 4097 4839 4924 5995 1391
Size: 399 1958 36 313 742 85 1071 2174
Fragments arranged by size:
2174 1958 1071 742 399 313 85 36
BspEI T'CCGG_A
Cuts at: 6441 6441
Size: 6778
BspHI T'CATG_A
Cuts at: 4667 6397 4667
Size: 1730 5048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3455 4133 844
Size: 2611 678 3489
Fragments arranged by size:
3489 2611 678
BsrBI CCG'CTC
Cuts at: 1394 1823 4665 1394
Size: 429 2842 3507
Fragments arranged by size:
3507 2842 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 2658 5273 5447 65
Size: 1183 835 575 2615 174 1396
Fragments arranged by size:
2615 1396 1183 835 575 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5422 5609 1700
Size: 110 3612 187 2869
Fragments arranged by size:
3612 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3593 96
Size: 3497 3281
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6778
BstBI TT'CG_AA
Cuts at: 5582 5582
Size: 6778
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6778
BstYI r'GATC_y
Cuts at: 1972 2267 2684 2725 4108 4475 4860 4877 6329
Size: 295 417 41 1383 367 385 17 1452
Cuts at: 6329 6415 6427 6773 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1383 417 385 367 346 295
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5287
Fragments arranged by size:
5287 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 4294 5088 5108 4294
Size: 794 20 5964
Fragments arranged by size:
5964 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3805 3840 529
Size: 35 3241 35 3467
Fragments arranged by size:
3467 3241 35 35
DraI TTT'AAA
Cuts at: 2493 2992 4082 2493
Size: 499 1090 5189
Fragments arranged by size:
5189 1090 499
DraIII CAC_nnn'GTG
Cuts at: 2135 4076 2135
Size: 1941 4837
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4225 5114 6743
Size: 54 1425 50 275 18 2395 889 1629
Cuts at: 6743 8
Size: 43
Fragments arranged by size:
2395 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2197 4708 1353
Size: 844 2511 3423
Fragments arranged by size:
3423 2511 844
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5365 5739 5885 387
Size: 1064 138 3776 374 146 1280
Fragments arranged by size:
3776 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 3524 4841 5438 6225 6493 1239
Size: 393 402 1490 1317 597 787 268 1524
Fragments arranged by size:
1524 1490 1317 787 597 402 393 268
EcoRI G'AATT_C
Cuts at: 3756 4087 4465 3756
Size: 331 378 6069
Fragments arranged by size:
6069 378 331
EcoRV GAT'ATC
Cuts at: 2187 3970 2187
Size: 1783 4995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2895 2110
Size: 785 5993
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 5925 1678
Size: 4247 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3174 3206 5419 5451 5493
Size: 32 135 32 1602 32 2213 32 42
Cuts at: 5493 5525 1373
Size: 32 2626
Fragments arranged by size:
2626 2213 1602 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4122 4368 669
Size: 988 281 2184 246 3079
Fragments arranged by size:
3079 2184 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2218 2877 748
Size: 1470 659 4649
Fragments arranged by size:
4649 1470 659
HpaI GTT'AAC
Cuts at: 4368 4368
Size: 6778
KpnI G_GTAC'C
Cuts at: 4103 4103
Size: 6778
MfeI C'AATT_G
Cuts at: 3394 4377 3394
Size: 983 5795
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2749 3199 5892 6076 1305
Size: 1444 450 2693 184 2007
Fragments arranged by size:
2693 2007 1444 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4956
Fragments arranged by size:
4956 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2512 3360 3567 4736 5095 5254 518
Size: 648 1346 848 207 1169 359 159 2042
Fragments arranged by size:
2042 1346 1169 848 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 4194 4871 6019 6264 1606
Size: 2588 677 1148 245 2120
Fragments arranged by size:
2588 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5287
Fragments arranged by size:
5287 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NsiI A_TGCA'T
Cuts at: 3979 3979
Size: 6778
NspI r_CATG'y
Cuts at: 1132 1229 2515 2897 3671 4136 5681 1132
Size: 97 1286 382 774 465 1545 2229
Fragments arranged by size:
2229 1545 1286 774 465 382 97
PacI TTA_AT'TAA
Cuts at: 2436 2436
Size: 6778
PasI CC'CwG_GG
Cuts at: 1348 2680 1348
Size: 1332 5446
PciI A'CATG_T
Cuts at: 2511 3667 5677 2511
Size: 1156 2010 3612
Fragments arranged by size:
3612 2010 1156
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 3425 3733 1108
Size: 1561 756 308 4153
Fragments arranged by size:
4153 1561 756 308
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PmlI CAC'GTG
Cuts at: 3833 3833
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4847 4879 6360 6392 4847
Size: 32 1481 32 5233
Fragments arranged by size:
5233 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6778
PsiI TTA'TAA
Cuts at: 4348 4348
Size: 6778
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 6746
PstI C_TGCA'G
Cuts at: 830 3518 4130 830
Size: 2688 612 3478
Fragments arranged by size:
3478 2688 612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SbfI CC_TGCA'GG
Cuts at: 4130 4130
Size: 6778
ScaI AGT'ACT
Cuts at: 5026 6738 5026
Size: 1712 5066
SfcI C'TryA_G
Cuts at: 826 1045 3498 3514 4126 5261 5942 6133 826
Size: 219 2453 16 612 1135 681 191 1471
Fragments arranged by size:
2453 1471 1135 681 612 219 191 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 4105 1872
Size: 2233 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4882 5783 6045 820
Size: 197 926 2939 901 262 1553
Fragments arranged by size:
2939 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3091 4569 493
Size: 2598 1478 2702
Fragments arranged by size:
2702 2598 1478
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6778
SphI G_CATG'C
Cuts at: 1132 4136 1132
Size: 3004 3774
SspI AAT'ATT
Cuts at: 5 52 2956 4702 5
Size: 47 2904 1746 2081
Fragments arranged by size:
2904 2081 1746 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6778
StyI C'CwwG_G
Cuts at: 513 1065 2004 4217 513
Size: 552 939 2213 3074
Fragments arranged by size:
3074 2213 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3557 4805 4822 4975 5160 5605 325
Size: 1255 1977 1248 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 1248 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3593 5024 6736
Size: 274 80 33 51 1443 1616 1431 1712
Cuts at: 6736 96
Size: 138
Fragments arranged by size:
1712 1616 1443 1431 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3708 3740 6738 6770 3708
Size: 32 2998 32 3716
Fragments arranged by size:
3716 2998 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6778
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2572
Size: 6778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6778
XmaI C'CCGG_G
Cuts at: 1870 4103 1870
Size: 2233 4545
XmnI GAAnn'nnTTC
Cuts at: 2090 2878 4907 2090
Size: 788 2029 3961
Fragments arranged by size:
3961 2029 788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4586 276
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI