Restriction map for
pFN21AE2388
(Circular) (Six-base) MAPSORT of: pFN21AE2388.seq Check: 375 from: 1 to: 9157
pFN21AE2388 9157 bp
With 182 enzymes: SgfI *
February 7, 2012 16:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4669 4669
Size: 9157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6967 278
Size: 53 83 186 936 5431 2468
Fragments arranged by size:
5431 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 3563 4983 6500 1937
Size: 1218 408 1420 1517 4594
Fragments arranged by size:
4594 1517 1420 1218 408
Acc65I G'GTAC_C
Cuts at: 6478 6478
Size: 9157
AclI AA'CG_TT
Cuts at: 1563 5321 7284 7657 1563
Size: 3758 1963 373 3063
Fragments arranged by size:
3758 3063 1963 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 4956 7220 8604 8872 1632
Size: 548 2776 2264 1384 268 1917
Fragments arranged by size:
2776 2264 1917 1384 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8960
AflIII A'CryG_T
Cuts at: 1203 8056 9033 1203
Size: 6853 977 1327
Fragments arranged by size:
6853 1327 977
AgeI A'CCGG_T
Cuts at: 7988 7988
Size: 9157
AhdI GACnn_n'nnGTC
Cuts at: 2932 3162 4514 7886 2932
Size: 230 1352 3372 4203
Fragments arranged by size:
4203 3372 1352 230
AleI CACnn'nnGTG
Cuts at: 1166 2342 1166
Size: 1176 7981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5339 1184
Size: 4155 5002
AlwNI CAG_nnn'CTG
Cuts at: 2326 3995 4841 5861 6900 8472 2326
Size: 1669 846 1020 1039 1572 3011
Fragments arranged by size:
3011 1669 1572 1039 1020 846
ApaI G_GGCC'C
Cuts at: 4175 4427 5072 5495 4175
Size: 252 645 423 7837
Fragments arranged by size:
7837 645 423 252
ApaLI G'TGCA_C
Cuts at: 7214 8370 7214
Size: 1156 8001
ApoI r'AATT_y
Cuts at: 1493 2671 3497 3650 4323 5446 5948 6466 6692
Size: 1178 826 153 673 1123 502 518 226
Cuts at: 6692 6844 8953 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1178 1123 826 673 518 502
226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 7711 160
Size: 872 6679 1606
Fragments arranged by size:
6679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9157
AvaI C'yCGr_G
Cuts at: 1870 1943 4037 4497 6482 1870
Size: 73 2094 460 1985 4545
Fragments arranged by size:
4545 2094 1985 460 73
AvrII C'CTAG_G
Cuts at: 2912 2912
Size: 9157
BamHI G'GATC_C
Cuts at: 4184 6487 6854 4184
Size: 2303 367 6487
Fragments arranged by size:
6487 2303 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3928 4882 6415 6478 7933
Size: 325 217 627 2141 954 1533 63 1455
Cuts at: 7933 618
Size: 1842
Fragments arranged by size:
2141 1842 1533 1455 954 627 325 217
63
BanII G_rGCy'C
Cuts at: 1391 2046 2280 3237 4175 4288 4427 5045 5072
Size: 655 234 957 938 113 139 618 27
Cuts at: 5072 5079 5495 5996 6476 1391
Size: 7 416 501 480 4072
Fragments arranged by size:
4072 957 938 655 618 501 480 416
234 139 113 27 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 2457 2617 3521 928
Size: 971 487 71 160 904 6564
Fragments arranged by size:
6564 971 904 487 160 71
BbvCI CC'TCA_GC
Cuts at: 6117 6117
Size: 9157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2845 2879 3135 3169 4575 4609 7348
Size: 34 908 34 256 34 1406 34 2739
Cuts at: 7348 7382 1903
Size: 34 3678
Fragments arranged by size:
3678 2739 1406 908 256 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7049 8259 7049
Size: 1210 7947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5313 7768 136
Size: 107 122 71 631 605 3641 2455 1525
Fragments arranged by size:
3641 2455 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2850 9152 2850
Size: 6302 2855
BlpI GC'TnA_GC
Cuts at: 6574 6574
Size: 9157
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2467 3872 4175 4427 4885 5072 5495
Size: 222 455 1405 303 252 458 187 423
Cuts at: 5495 7218 8374 1790
Size: 1723 1156 2573
Fragments arranged by size:
2573 1723 1405 1156 458 455 423 303
252 222 187
BmgBI CAC'GTC
Cuts at: 1318 2161 3349 1318
Size: 843 1188 7126
Fragments arranged by size:
7126 1188 843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4635 6888 7846
Size: 416 53 83 399 24 3218 2253 958
Cuts at: 7846 442
Size: 1753
Fragments arranged by size:
3218 2253 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2812 2065
Size: 747 8410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3157 3225 3645 3718 4038 4065 4224 4272
Size: 1918 68 420 73 320 27 159 48
Cuts at: 4272 4611 5595 7817 1239
Size: 339 984 2222 2579
Fragments arranged by size:
2579 2222 1918 984 420 339 320 159
73 68 48 27
Bpu10I CC'TnA_GC
Cuts at: 3194 6117 3194
Size: 2923 6234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2560 3207 7282 8147 8445 2560
Size: 647 4075 865 298 3272
Fragments arranged by size:
4075 3272 865 647 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4501 5181 5698 7820 882
Size: 617 3002 680 517 2122 2219
Fragments arranged by size:
3002 2219 2122 680 617 517
BsaAI yAC'GTr
Cuts at: 493 1204 4486 6948 493
Size: 711 3282 2462 2702
Fragments arranged by size:
3282 2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 9151 9151
Size: 9157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6964 7346 7981 275
Size: 53 83 186 936 5431 382 635 1451
Fragments arranged by size:
5431 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4885 7590 7988 8262 8409 8820 4885
Size: 2705 398 274 147 411 5222
Fragments arranged by size:
5222 2705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2633 2663 5385 5415 6088
Size: 30 165 30 1230 30 2722 30 673
Cuts at: 6088 6118 1178
Size: 30 4217
Fragments arranged by size:
4217 2722 1230 673 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2144 2654 2816 2972 3017 3104 3199
Size: 844 114 510 162 156 45 87 95
Cuts at: 3199 4003 4142 4729 5080 6018 6425 1186
Size: 804 139 587 351 938 407 3918
Fragments arranged by size:
3918 938 844 804 587 510 407 351
162 156 139 114 95 87 45
BseYI C'CCAG_C
Cuts at: 2035 2271 3698 3920 4331 5164 5369 5515 5529
Size: 236 1427 222 411 833 205 146 14
Cuts at: 5529 5740 6198 8360 8856 2035
Size: 211 458 2162 496 2336
Fragments arranged by size:
2336 2162 1427 833 496 458 411 236
222 211 205 146 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 3056 3139 4070 4703 4770 5014
Size: 573 603 717 83 931 633 67 244
Cuts at: 5014 1163
Size: 5306
Fragments arranged by size:
5306 931 717 633 603 573 244 83
67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 4349 4589 6125 6607 7368
Size: 275 186 30 2318 240 1536 482 761
Cuts at: 7368 8396 1540
Size: 1028 2301
Fragments arranged by size:
2318 2301 1536 1028 761 482 275 240
186 30
BsiHKAI G_wGCw'C
Cuts at: 2046 2280 3237 4660 5045 5079 5996 7218 7303
Size: 234 957 1423 385 34 917 1222 85
Cuts at: 7303 8374 2046
Size: 1071 2829
Fragments arranged by size:
2829 1423 1222 1071 957 917 385 234
85 34
BsmI GAATG_Cn'
Cuts at: 5791 5827 6348 6668 6761 5791
Size: 36 521 320 93 8187
Fragments arranged by size:
8187 521 320 93 36
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2046 2280 2467 3237 3872 4175
Size: 399 222 34 234 187 770 635 303
Cuts at: 4175 4288 4427 4660 4885 5045 5072 5079 5495
Size: 113 139 233 225 160 27 7 416
Cuts at: 5495 5996 6476 7218 7303 8374 1391
Size: 501 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 635 501 480 416
399 303 234 233 225 222 187 160
139 113 85 34 27 7
BspEI T'CCGG_A
Cuts at: 8820 8820
Size: 9157
BspHI T'CATG_A
Cuts at: 4503 7046 8776 4503
Size: 2543 1730 4884
Fragments arranged by size:
4884 2543 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 4669 4807 6512 844
Size: 1490 2335 138 1705 3489
Fragments arranged by size:
3489 2335 1705 1490 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2049 2144 3732 7044 1394
Size: 429 226 95 1588 3312 3507
Fragments arranged by size:
3507 3312 1588 429 226 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 3612 5124 7652 7826 65
Size: 1183 2364 1512 2528 174 1396
Fragments arranged by size:
2528 2364 1512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 6124 7801 7988 1700
Size: 110 563 3751 1677 187 2869
Fragments arranged by size:
3751 2869 1677 563 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 6327 96
Size: 2400 3831 2926
Fragments arranged by size:
3831 2926 2400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9157
BssSI C'ACGA_G
Cuts at: 7217 8229 7217
Size: 1012 8145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5861 794
Size: 5067 4090
BstBI TT'CG_AA
Cuts at: 7961 7961
Size: 9157
BstEII G'GTnAC_C
Cuts at: 1184 2770 1184
Size: 1586 7571
BstYI r'GATC_y
Cuts at: 1972 2349 2850 3991 4184 6487 6854 7239 7256
Size: 377 501 1141 193 2303 367 385 17
Cuts at: 7256 8708 8794 8806 9152 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2303 1977 1452 1141 501 385 377 367
346 193 86 17 12
BstZ17I GTA'TAC
Cuts at: 3564 3564
Size: 9157
Bsu36I CC'TnA_GG
Cuts at: 4573 4573
Size: 9157
BtgI C'CryG_G
Cuts at: 513 1065 3438 3771 3857 4396 5196 5790 513
Size: 552 2373 333 86 539 800 594 3880
Fragments arranged by size:
3880 2373 800 594 552 539 333 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2056 4739 487
Size: 683 56 830 2683 4905
Fragments arranged by size:
4905 2683 830 683 56
BtsI GCAGTG_nn'
Cuts at: 3039 3111 3127 3324 5290 5862 6673 7467 7487
Size: 72 16 197 1966 572 811 794 20
Cuts at: 7487 3039
Size: 4709
Fragments arranged by size:
4709 1966 811 794 572 197 72 20
16
ClaI AT'CG_AT
Cuts at: 1554 4345 1554
Size: 2791 6366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9122
DraI TTT'AAA
Cuts at: 3939 6461 3939
Size: 2522 6635
DraIII CAC_nnn'GTG
Cuts at: 4961 6085 4961
Size: 1124 8033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8164 809
Size: 507 6848 1802
Fragments arranged by size:
6848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2067 3880
Size: 54 1425 50 275 18 198 39 1813
Cuts at: 3880 4295 5793 6122 6126 6604 7493 9122 8
Size: 415 1498 329 4 478 889 1629 43
Fragments arranged by size:
1813 1629 1498 1425 889 478 415 329
275 198 54 50 43 39 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2028 6122 6604 1537
Size: 275 216 4094 482 4090
Fragments arranged by size:
4094 4090 482 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2209 2337 2599 2748 3006 3063 3176
Size: 798 58 128 262 149 258 57 113
Cuts at: 3176 3823 4677 4941 5777 5898 6160 7087 1353
Size: 647 854 264 836 121 262 927 3423
Fragments arranged by size:
3423 927 854 836 798 647 264 262
262 258 149 128 121 113 58 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 6021 7744 8118 8264 387
Size: 1064 138 507 3925 1723 374 146 1280
Fragments arranged by size:
3925 1723 1280 1064 507 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2278 3235 5043 5077 5994 2044
Size: 234 957 1808 34 917 5207
Fragments arranged by size:
5207 1808 957 917 234 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 3157 3225 3645 3718 4038 4065
Size: 393 548 977 68 420 73 320 27
Cuts at: 4065 4224 4272 4611 4956 5595 7220 7817 8604
Size: 159 48 339 345 639 1625 597 787
Cuts at: 8604 8872 1239
Size: 268 1524
Fragments arranged by size:
1625 1524 977 787 639 597 548 420
393 345 339 320 268 159 73 68
48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2831 3981 4572 6215 2195
Size: 636 1150 591 1643 5137
Fragments arranged by size:
5137 1643 1150 636 591
EcoO109I rG'GnC_Cy
Cuts at: 2062 2323 2410 2506 2706 2793 3433 3739 4106
Size: 261 87 96 200 87 640 306 367
Cuts at: 4106 4415 4454 5272 5492 5766 6101 6224 6311
Size: 309 39 818 220 274 335 123 87
Cuts at: 6311 2062
Size: 4908
Fragments arranged by size:
4908 818 640 367 335 309 306 274
261 220 200 123 96 87 87 87
39
EcoRI G'AATT_C
Cuts at: 5446 6466 6844 5446
Size: 1020 378 7759
Fragments arranged by size:
7759 1020 378
EcoRV GAT'ATC
Cuts at: 2221 2221
Size: 9157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 6330 2162
Size: 4168 4989
FseI GG_CCGG'CC
Cuts at: 6128 6128
Size: 9157
FspI TGC'GCA
Cuts at: 7663 7663
Size: 9157
HaeII r_GCGC'y
Cuts at: 1678 3815 5217 8304 1678
Size: 2137 1402 3087 2531
Fragments arranged by size:
3087 2531 2137 1402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2918 2950 3148 3180 4500
Size: 32 135 32 1346 32 198 32 1320
Cuts at: 4500 4532 6088 6120 7798 7830 7872 7904 1373
Size: 32 1556 32 1678 32 42 32 2626
Fragments arranged by size:
2626 1678 1556 1346 1320 198 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 6501 6747 669
Size: 988 281 1218 3345 246 3079
Fragments arranged by size:
3345 3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9157
HpaI GTT'AAC
Cuts at: 6747 6747
Size: 9157
KpnI G_GTAC'C
Cuts at: 6482 6482
Size: 9157
MfeI C'AATT_G
Cuts at: 6756 6756
Size: 9157
MluI A'CGCG_T
Cuts at: 9033 9033
Size: 9157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3066 3291 4066 5864 6458 8271 8455 1305
Size: 1761 225 775 1798 594 1813 184 2007
Fragments arranged by size:
2007 1813 1798 1761 775 594 225 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5795 10
Size: 54 1425 343 3963 3372
Fragments arranged by size:
3963 3372 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 2342 3329 3909 7115 7474 7633
Size: 648 988 188 987 580 3206 359 159
Cuts at: 7633 518
Size: 2042
Fragments arranged by size:
3206 2042 988 987 648 580 359 188
159
MspA1I CmG'CkG
Cuts at: 1606 2271 3859 5419 5453 6121 6573 7250 8398
Size: 665 1588 1560 34 668 452 677 1148
Cuts at: 8398 8643 1606
Size: 245 2120
Fragments arranged by size:
2120 1588 1560 1148 677 668 665 452
245 34
NaeI GCC'GGC
Cuts at: 6126 6126
Size: 9157
NcoI C'CATG_G
Cuts at: 513 1065 3438 3771 4396 5790 513
Size: 552 2373 333 625 1394 3880
Fragments arranged by size:
3880 2373 1394 625 552 333
NdeI CA'TA_TG
Cuts at: 387 4338 387
Size: 3951 5206
NgoMIV G'CCGG_C
Cuts at: 6124 6124
Size: 9157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9157
NotI GC'GGCC_GC
Cuts at: 6604 6604
Size: 9157
NruI TCG'CGA
Cuts at: 2787 2787
Size: 9157
NspI r_CATG'y
Cuts at: 1132 1229 3150 4234 4585 6515 8060 1132
Size: 97 1921 1084 351 1930 1545 2229
Fragments arranged by size:
2229 1930 1921 1545 1084 351 97
PasI CC'CwG_GG
Cuts at: 1348 4864 4874 5719 6144 6188 1348
Size: 3516 10 845 425 44 4317
Fragments arranged by size:
4317 3516 845 425 44 10
PciI A'CATG_T
Cuts at: 8056 8056
Size: 9157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4181 4530 4964 5573 1108
Size: 3073 349 434 609 4692
Fragments arranged by size:
4692 3073 609 434 349
PfoI T'CCnGG_A
Cuts at: 2319 2584 3622 4023 6318 2319
Size: 265 1038 401 2295 5158
Fragments arranged by size:
5158 2295 1038 401 265
PmeI GTTT'AAAC
Cuts at: 6461 6461
Size: 9157
PmlI CAC'GTG
Cuts at: 4486 4486
Size: 9157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2799 2831 7226 7258 8739 8771 2799
Size: 32 4395 32 1481 32 3185
Fragments arranged by size:
4395 3185 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2323 2506 2706 2793 3739 4106 4415 4454
Size: 261 183 200 87 946 367 309 39
Cuts at: 4454 5766 6224 6311 2062
Size: 1312 458 87 4908
Fragments arranged by size:
4908 1312 946 458 367 309 261 200
183 87 87 39
PshAI GACnn'nnGTC
Cuts at: 1613 2704 1613
Size: 1091 8066
PsiI TTA'TAA
Cuts at: 6727 6727
Size: 9157
PspOMI G'GGCC_C
Cuts at: 4171 4423 5068 5491 4171
Size: 252 645 423 7837
Fragments arranged by size:
7837 645 423 252
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5272 5304 5272
Size: 32 9125
PstI C_TGCA'G
Cuts at: 830 2637 5022 5484 6096 6509 830
Size: 1807 2385 462 612 413 3478
Fragments arranged by size:
3478 2385 1807 612 462 413
PvuI CG_AT'CG
Cuts at: 2001 4349 2001
Size: 2348 6809
PvuII CAG'CTG
Cuts at: 5453 5453
Size: 9157
SacI G_AGCT'C
Cuts at: 2046 2280 3237 5045 5079 5996 2046
Size: 234 957 1808 34 917 5207
Fragments arranged by size:
5207 1808 957 917 234 34
SacII CC_GC'GG
Cuts at: 3860 3860
Size: 9157
SalI G'TCGA_C
Cuts at: 1936 3154 6499 1936
Size: 1218 3345 4594
Fragments arranged by size:
4594 3345 1218
SanDI GG'GwC_CC
Cuts at: 2706 3739 6224 2706
Size: 1033 2485 5639
Fragments arranged by size:
5639 2485 1033
SapI GCTCTTCn'nnn_
Cuts at: 2151 3006 4677 2151
Size: 855 1671 6631
Fragments arranged by size:
6631 1671 855
SbfI CC_TGCA'GG
Cuts at: 5484 6509 5484
Size: 1025 8132
ScaI AGT'ACT
Cuts at: 7405 9117 7405
Size: 1712 7445
SexAI A'CCwGG_T
Cuts at: 4957 4957
Size: 9157
SfcI C'TryA_G
Cuts at: 826 1045 2633 3168 5018 5480 6092 6505 7640
Size: 219 1588 535 1850 462 612 413 1135
Cuts at: 7640 8321 8512 826
Size: 681 191 1471
Fragments arranged by size:
1850 1588 1471 1135 681 612 535 462
413 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9157
SmaI CCC'GGG
Cuts at: 1872 4039 6484 1872
Size: 2167 2445 4545
Fragments arranged by size:
4545 2445 2167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2575 3222 7261 8162 8424 820
Size: 197 926 632 647 4039 901 262 1553
Fragments arranged by size:
4039 1553 926 901 647 632 262 197
SnaBI TAC'GTA
Cuts at: 493 6948 493
Size: 6455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9157
SphI G_CATG'C
Cuts at: 1132 6515 1132
Size: 5383 3774
SspI AAT'ATT
Cuts at: 5 52 7081 5
Size: 47 7029 2081
Fragments arranged by size:
7029 2081 47
StuI AGG'CCT
Cuts at: 1517 5478 6252 6371 1517
Size: 3961 774 119 4303
Fragments arranged by size:
4303 3961 774 119
StyI C'CwwG_G
Cuts at: 513 1065 2676 2710 2722 2912 3438 3489 3771
Size: 552 1611 34 12 190 526 51 282
Cuts at: 3771 4396 4411 4419 5173 5275 5543 5762 5790
Size: 625 15 8 754 102 268 219 28
Cuts at: 5790 6596 513
Size: 806 3074
Fragments arranged by size:
3074 1611 806 754 625 552 526 282
268 219 190 102 51 34 28 15
12 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 3934 4469 4654 4842 5580 5640
Size: 1255 855 1499 535 185 188 738 60
Cuts at: 5640 6201 6212 7184 7201 7354 7539 7984 325
Size: 561 11 972 17 153 185 445 1498
Fragments arranged by size:
1499 1498 1255 972 855 738 561 535
445 188 185 185 153 60 17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4369 6327
Size: 274 80 33 51 1443 519 1873 1958
Cuts at: 6327 7403 9115 96
Size: 1076 1712 138
Fragments arranged by size:
1958 1873 1712 1443 1076 519 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 3353 3385 4169 4201 4384 4416 5385
Size: 32 220 32 784 32 183 32 969
Cuts at: 5385 5417 9117 9149 3101
Size: 32 3700 32 3109
Fragments arranged by size:
3700 3109 969 784 220 183 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 3690 1316
Size: 1074 1300 6783
Fragments arranged by size:
6783 1300 1074
XbaI T'CTAG_A
Cuts at: 3343 6493 3343
Size: 3150 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4861 5570 4861
Size: 709 8448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9157
XmaI C'CCGG_G
Cuts at: 1870 4037 6482 1870
Size: 2167 2445 4545
Fragments arranged by size:
4545 2445 2167
XmnI GAAnn'nnTTC
Cuts at: 7286 7286
Size: 9157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6965 276
Size: 53 83 186 936 5431 2468
Fragments arranged by size:
5431 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstXI FspAI KasI NarI NsiI PacI PspXI RsrII
SfiI SfoI SrfI SwaI