Restriction map for
pFN21AE2387
(Circular) (Six-base) MAPSORT of: pFN21AE2387.seq Check: 1945 from: 1 to: 9973
pFN21AE2387 9973 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3154 4144 6972 3154
Size: 990 2828 6155
Fragments arranged by size:
6155 2828 990
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6609 7783 278
Size: 53 83 186 936 5073 1174 2468
Fragments arranged by size:
5073 2468 1174 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7316 1937
Size: 5379 4594
Acc65I G'GTAC_C
Cuts at: 3066 7294 3066
Size: 4228 5745
AclI AA'CG_TT
Cuts at: 1563 8100 8473 1563
Size: 6537 373 3063
Fragments arranged by size:
6537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2354 3497 4325 4566 5151 5732 8036
Size: 370 352 1143 828 241 585 581 2304
Cuts at: 8036 9420 9688 1632
Size: 1384 268 1917
Fragments arranged by size:
2304 1917 1384 1143 828 585 581 370
352 268 241
AfeI AGC'GCT
Cuts at: 2666 3319 6291 2666
Size: 653 2972 6348
Fragments arranged by size:
6348 2972 653
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9776
AflIII A'CryG_T
Cuts at: 1203 7053 8872 9849 1203
Size: 5850 1819 977 1327
Fragments arranged by size:
5850 1819 1327 977
AgeI A'CCGG_T
Cuts at: 3063 8804 3063
Size: 5741 4232
AhdI GACnn_n'nnGTC
Cuts at: 7144 8702 7144
Size: 1558 8415
AleI CACnn'nnGTG
Cuts at: 1166 4505 1166
Size: 3339 6634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2130 3645 4956 1184
Size: 946 1515 1311 6201
Fragments arranged by size:
6201 1515 1311 946
AlwNI CAG_nnn'CTG
Cuts at: 2605 2638 2845 3007 3145 3250 3374 3526 3814
Size: 33 207 162 138 105 124 152 288
Cuts at: 3814 3929 3967 4573 4582 4861 4903 5221 5839
Size: 115 38 606 9 279 42 318 618
Cuts at: 5839 6283 6430 7246 7716 9288 2605
Size: 444 147 816 470 1572 3290
Fragments arranged by size:
3290 1572 816 618 606 470 444 318
288 279 207 162 152 147 138 124
115 105 42 38 33 9
ApaI G_GGCC'C
Cuts at: 2544 5020 2544
Size: 2476 7497
ApaLI G'TGCA_C
Cuts at: 2897 4115 8030 9186 2897
Size: 1218 3915 1156 3684
Fragments arranged by size:
3915 3684 1218 1156
ApoI r'AATT_y
Cuts at: 1493 2773 6647 7282 7508 7660 9769 1493
Size: 1280 3874 635 226 152 2109 1697
Fragments arranged by size:
3874 2109 1697 1280 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8527 160
Size: 872 7495 1606
Fragments arranged by size:
7495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9973
AvaI C'yCGr_G
Cuts at: 1870 1943 2861 4051 5082 6443 6896 7298 1870
Size: 73 918 1190 1031 1361 453 402 4545
Fragments arranged by size:
4545 1361 1190 1031 918 453 402 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6254 6287 6254
Size: 33 9940
BamHI G'GATC_C
Cuts at: 2401 3585 4831 7303 7670 2401
Size: 1184 1246 2472 367 4704
Fragments arranged by size:
4704 2472 1246 1184 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2760 3066 3169 3746 4825
Size: 325 217 627 973 306 103 577 1079
Cuts at: 4825 5078 5615 7294 8749 618
Size: 253 537 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 1079 973 627 577 537
325 306 253 217 103
BanII G_rGCy'C
Cuts at: 1391 2085 2544 3309 4266 5020 6033 6444 6929
Size: 694 459 765 957 754 1013 411 485
Cuts at: 6929 7041 7292 1391
Size: 112 251 4072
Fragments arranged by size:
4072 1013 957 765 754 694 485 459
411 251 112
BbeI G_GCGC'C
Cuts at: 2764 5082 2764
Size: 2318 7655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2991 3485 3698 5462 5910 928
Size: 971 1092 494 213 1764 448 4991
Fragments arranged by size:
4991 1764 1092 971 494 448 213
BbvCI CC'TCA_GC
Cuts at: 3708 3761 4283 6309 3708
Size: 53 522 2026 7372
Fragments arranged by size:
7372 2026 522 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2171 2205 6092 6126 8164 8198 1903
Size: 34 234 34 3887 34 2038 34 3678
Fragments arranged by size:
3887 3678 2038 234 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7865 9075 7865
Size: 1210 8763
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3638 5087 5126
Size: 107 122 71 631 605 1966 1449 39
Cuts at: 5126 8584 136
Size: 3458 1525
Fragments arranged by size:
3458 1966 1525 1449 631 605 122 107
71 39
BglII A'GATC_T
Cuts at: 2373 4362 5808 7095 9968 2373
Size: 1989 1446 1287 2873 2378
Fragments arranged by size:
2873 2378 1989 1446 1287
BlpI GC'TnA_GC
Cuts at: 5276 7390 5276
Size: 2114 7859
Bme1580I G_kGCm'C
Cuts at: 1790 2544 2901 3174 4119 4945 5020 8034 9190
Size: 754 357 273 945 826 75 3014 1156
Cuts at: 9190 1790
Size: 2573
Fragments arranged by size:
3014 2573 1156 945 826 754 357 273
75
BmgBI CAC'GTC
Cuts at: 1318 2212 2989 3133 1318
Size: 894 777 144 8158
Fragments arranged by size:
8158 894 777 144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2425 4248 7096
Size: 416 53 83 399 24 1008 1823 2848
Cuts at: 7096 7704 8662 442
Size: 608 958 1753
Fragments arranged by size:
2848 1823 1753 1008 958 608 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5445 1056
Size: 4389 5584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 6049 7114 4502
Size: 1547 1065 7361
Fragments arranged by size:
7361 1547 1065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2746 2813 3623 3875 4115 4604 4686 4751
Size: 1507 67 810 252 240 489 82 65
Cuts at: 4751 4862 4937 4967 5075 5222 5270 5603 5672
Size: 111 75 30 108 147 48 333 69
Cuts at: 5672 5999 6233 6314 6380 6452 6494 6593 6734
Size: 327 234 81 66 72 42 99 141
Cuts at: 6734 6755 6817 6869 8633 1239
Size: 21 62 52 1764 2579
Fragments arranged by size:
2579 1764 1507 810 489 333 327 252
240 234 147 141 111 108 99 82
81 75 72 69 67 66 65 62
52 48 42 30 21
Bpu10I CC'TnA_GC
Cuts at: 2199 3000 3708 3761 4283 5252 5756 6309 2199
Size: 801 708 53 522 969 504 553 5863
Fragments arranged by size:
5863 969 801 708 553 522 504 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3161 3185 3635 4792 4802 5282 5342 6221
Size: 225 24 450 1157 10 480 60 879
Cuts at: 6221 6545 6854 8098 8963 9261 2936
Size: 324 309 1244 865 298 3648
Fragments arranged by size:
3648 1244 1157 879 865 480 450 324
309 298 225 60 24 10
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 4437 4631 4949 6440 6731 6780
Size: 617 2790 148 194 318 1491 291 49
Cuts at: 6780 8636 882
Size: 1856 2219
Fragments arranged by size:
2790 2219 1856 1491 617 318 291 194
148 49
BsaAI yAC'GTr
Cuts at: 493 1204 4228 4468 4981 7764 493
Size: 711 3024 240 513 2783 2702
Fragments arranged by size:
3024 2783 2702 711 513 240
BsaBI GATnn'nnATC
Cuts at: 9967 9967
Size: 9973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2761 5079 5930 6606
Size: 53 83 186 936 1228 2318 851 676
Cuts at: 6606 7780 8162 8797 275
Size: 1174 382 635 1451
Fragments arranged by size:
2318 1451 1228 1174 936 851 676 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2784 3063 3414 3786 5631 6453 8406 8804 9078
Size: 279 351 372 1845 822 1953 398 274
Cuts at: 9078 9225 9636 2784
Size: 147 411 3121
Fragments arranged by size:
3121 1953 1845 822 411 398 372 351
279 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4664 4694 5195 5225 5591
Size: 30 165 30 3261 30 501 30 366
Cuts at: 5591 5621 5915 5945 6815 6845 1178
Size: 30 294 30 870 30 4306
Fragments arranged by size:
4306 3261 870 501 366 294 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2516 2819 2954 4592 4688 4721 4859
Size: 1261 69 303 135 1638 96 33 138
Cuts at: 4859 4895 4925 5063 5090 5282 5285 5504 5507
Size: 36 30 138 27 192 3 219 3
Cuts at: 5507 5636 5777 5936 5969 6038 6041 6095 6098
Size: 129 141 159 33 69 3 54 3
Cuts at: 6098 6230 6590 6593 7106 1186
Size: 132 360 3 513 4053
Fragments arranged by size:
4053 1638 1261 513 360 303 219 192
159 141 138 138 135 132 129 96
69 69 54 36 33 33 30 27
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2750 3300 3320 3437 3876 4123 4377 5196 9176
Size: 550 20 117 439 247 254 819 3980
Cuts at: 9176 9672 2750
Size: 496 3051
Fragments arranged by size:
3980 3051 819 550 496 439 254 247
117 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2653 2807 3158 3641 5534 6077
Size: 573 339 578 154 351 483 1893 543
Cuts at: 6077 6453 1163
Size: 376 4683
Fragments arranged by size:
4683 1893 578 573 543 483 376 351
339 154
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2853 3345 7423 8184 9212 1540
Size: 275 186 852 492 4078 761 1028 2301
Fragments arranged by size:
4078 2301 1028 852 761 492 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2205 2859 2901 3309 3704 3801 4052 4058
Size: 120 654 42 408 395 97 251 6
Cuts at: 4058 4083 4119 4206 4751 4818 5100 5358 6033
Size: 25 36 87 545 67 282 258 675
Cuts at: 6033 6444 6507 8034 8119 9190 2085
Size: 411 63 1527 85 1071 2868
Fragments arranged by size:
2868 1527 1071 675 654 545 411 408
395 282 258 251 120 97 87 85
67 63 42 36 25 6
BsmI GAATG_Cn'
Cuts at: 2119 7484 7577 2119
Size: 5365 93 4515
Fragments arranged by size:
5365 4515 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4956 5937 4956
Size: 981 8992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2205 2544 2859 2901 3174 3309
Size: 399 295 120 339 315 42 273 135
Cuts at: 3309 3704 3801 4052 4058 4083 4119 4206 4266
Size: 395 97 251 6 25 36 87 60
Cuts at: 4266 4751 4818 4945 5020 5100 5358 6033 6444
Size: 485 67 127 75 80 258 675 411
Cuts at: 6444 6507 6929 7041 7292 8034 8119 9190 1391
Size: 63 422 112 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 485 422 411 399
395 339 315 295 273 258 251 251
135 127 120 112 97 87 85 80
75 67 63 60 42 36 25 6
BspEI T'CCGG_A
Cuts at: 5631 6453 9636 5631
Size: 822 3183 5968
Fragments arranged by size:
5968 3183 822
BspHI T'CATG_A
Cuts at: 7862 9592 7862
Size: 1730 8243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2484 2934 3154 4144 4152 4329 6735
Size: 1517 123 450 220 990 8 177 2406
Cuts at: 6735 6798 6972 7328 844
Size: 63 174 356 3489
Fragments arranged by size:
3489 2406 1517 990 450 356 220 177
174 123 63 8
BsrBI CCG'CTC
Cuts at: 1394 1823 3199 3637 4717 5317 6205 6232 7860
Size: 429 1376 438 1080 600 888 27 1628
Cuts at: 7860 1394
Size: 3507
Fragments arranged by size:
3507 1628 1376 1080 888 600 438 429
27
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 8468 8642 65
Size: 1183 1026 6194 174 1396
Fragments arranged by size:
6194 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2757 3063 3561 6186 6417 6618 8617
Size: 110 947 306 498 2625 231 201 1999
Cuts at: 8617 8804 1700
Size: 187 2869
Fragments arranged by size:
2869 2625 1999 947 498 306 231 201
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9973
BssSI C'ACGA_G
Cuts at: 3896 6783 8033 9045 3896
Size: 2887 1250 1012 4824
Fragments arranged by size:
4824 2887 1250 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3145 3169 3805 4030 4529 6385 794
Size: 2351 24 636 225 499 1856 4382
Fragments arranged by size:
4382 2351 1856 636 499 225 24
BstBI TT'CG_AA
Cuts at: 8777 8777
Size: 9973
BstEII G'GTnAC_C
Cuts at: 1184 6325 1184
Size: 5141 4832
BstXI CCAn_nnnn'nTGG
Cuts at: 2465 3044 3214 3556 3853 5509 5875 2465
Size: 579 170 342 297 1656 366 6563
Fragments arranged by size:
6563 1656 579 366 342 297 170
BstYI r'GATC_y
Cuts at: 1972 2373 2401 3585 4362 4831 5808 5886 5974
Size: 401 28 1184 777 469 977 78 88
Cuts at: 5974 6549 7095 7303 7670 8055 8072 9524 9610
Size: 575 546 208 367 385 17 1452 86
Cuts at: 9610 9622 9968 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1184 977 777 575 546 469
401 385 367 346 208 88 86 78
28 17 12
Bsu36I CC'TnA_GG
Cuts at: 3375 4788 5660 3375
Size: 1413 872 7688
Fragments arranged by size:
7688 1413 872
BtgI C'CryG_G
Cuts at: 513 1065 3213 3975 4213 5322 6804 6930 513
Size: 552 2148 762 238 1109 1482 126 3556
Fragments arranged by size:
3556 2148 1482 1109 762 552 238 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9234
Fragments arranged by size:
9234 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4052 4081 4922 7489 8283 8303 3748
Size: 304 29 841 2567 794 20 5418
Fragments arranged by size:
5418 2567 841 794 304 29 20
ClaI AT'CG_AT
Cuts at: 1554 6129 1554
Size: 4575 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9938
DraI TTT'AAA
Cuts at: 7277 7277
Size: 9973
DraIII CAC_nnn'GTG
Cuts at: 2497 3227 2497
Size: 730 9243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7217 8980 809
Size: 507 5901 1763 1802
Fragments arranged by size:
5901 1802 1763 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2550 2850
Size: 54 1425 50 275 18 441 279 300
Cuts at: 2850 3342 3546 3593 3843 3888 3972 4038 4062
Size: 492 204 47 250 45 84 66 24
Cuts at: 4062 4216 5118 5325 5376 5540 6933 7420 8309
Size: 154 902 207 51 164 1393 487 889
Cuts at: 8309 9938 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1393 902 889 492 487 441
300 279 275 250 207 204 164 154
84 66 54 51 50 47 45 43
24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2850 3342 7420 1537
Size: 275 1038 492 4078 4090
Fragments arranged by size:
4090 4078 1038 492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2021 3854 4440 4509 5127 5132 5997
Size: 665 3 1833 586 69 618 5 865
Cuts at: 5997 6402 6687 7164 7903 1353
Size: 405 285 477 739 3423
Fragments arranged by size:
3423 1833 865 739 665 618 586 477
405 285 69 5 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 2315 5039 5567 5570 6497
Size: 1064 138 690 36 2724 528 3 927
Cuts at: 6497 6824 8560 8934 9080 387
Size: 327 1736 374 146 1280
Fragments arranged by size:
2724 1736 1280 1064 927 690 528 374
327 146 138 36 3
EcoICRI GAG'CTC
Cuts at: 2083 3307 6031 6442 2083
Size: 1224 2724 411 5614
Fragments arranged by size:
5614 2724 1224 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2354 2746 2813 3497 3623 3875
Size: 393 370 352 392 67 684 126 252
Cuts at: 3875 4115 4325 4566 4604 4686 4751 4862 4937
Size: 240 210 241 38 82 65 111 75
Cuts at: 4937 4967 5075 5151 5222 5270 5603 5672 5732
Size: 30 108 76 71 48 333 69 60
Cuts at: 5732 5999 6233 6314 6380 6452 6494 6593 6734
Size: 267 234 81 66 72 42 99 141
Cuts at: 6734 6755 6817 6869 8036 8633 9420 9688 1239
Size: 21 62 52 1167 597 787 268 1524
Fragments arranged by size:
1524 1167 787 684 597 393 392 370
352 333 268 267 252 241 240 234
210 141 126 111 108 99 82 81
76 75 72 71 69 67 66 65
62 60 52 48 42 38 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2222 2552 4013 4949 6575 6996 2222
Size: 330 1461 936 1626 421 5199
Fragments arranged by size:
5199 1626 1461 936 421 330
EcoO109I rG'GnC_Cy
Cuts at: 2541 3247 3379 3646 3730 3765 4784 4993 5016
Size: 706 132 267 84 35 1019 209 23
Cuts at: 5016 5656 5672 5836 6358 7109 7174 2541
Size: 640 16 164 522 751 65 5340
Fragments arranged by size:
5340 1019 751 706 640 522 267 209
164 132 84 65 35 23 16
EcoRI G'AATT_C
Cuts at: 6647 7282 7660 6647
Size: 635 378 8960
Fragments arranged by size:
8960 635 378
EcoRV GAT'ATC
Cuts at: 2323 2323
Size: 9973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4544 6650 4544
Size: 2106 7867
FspI TGC'GCA
Cuts at: 3014 5000 8479 3014
Size: 1986 3479 4508
Fragments arranged by size:
4508 3479 1986
HaeII r_GCGC'y
Cuts at: 1678 2668 2764 3321 4102 4188 5082 5242 6187
Size: 990 96 557 781 86 894 160 945
Cuts at: 6187 6293 6922 6973 9120 1678
Size: 106 629 51 2147 2531
Fragments arranged by size:
2531 2147 990 945 894 781 629 557
160 106 96 86 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3472 3504 5915 5947 7130
Size: 32 135 32 1900 32 2411 32 1183
Cuts at: 7130 7162 7186 7218 8614 8646 8688 8720 1373
Size: 32 24 32 1396 32 42 32 2626
Fragments arranged by size:
2626 2411 1900 1396 1183 135 42 32
32 32 32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2413 7222 7317 7563 669
Size: 988 281 475 4809 95 246 3079
Fragments arranged by size:
4809 3079 988 475 281 246 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9973
HpaI GTT'AAC
Cuts at: 7563 7563
Size: 9973
KasI G'GCGC_C
Cuts at: 2760 5078 2760
Size: 2318 7655
KpnI G_GTAC'C
Cuts at: 3070 7298 3070
Size: 4228 5745
MfeI C'AATT_G
Cuts at: 7572 7572
Size: 9973
MluI A'CGCG_T
Cuts at: 9849 9849
Size: 9973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 6415 9087 9271 1305
Size: 906 4204 2672 184 2007
Fragments arranged by size:
4204 2672 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 3548 3845 3890 4064
Size: 54 1425 343 720 996 297 45 174
Cuts at: 4064 10
Size: 5919
Fragments arranged by size:
5919 1425 996 720 343 297 174 54
45
MslI CAynn'nnrTG
Cuts at: 518 1166 3212 4505 6131 6803 7058 7931 8290
Size: 648 2046 1293 1626 672 255 873 359
Cuts at: 8290 8449 518
Size: 159 2042
Fragments arranged by size:
2046 2042 1626 1293 873 672 648 359
255 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2332 2482 2842 3437 3463 3523 3811
Size: 498 228 150 360 595 26 60 288
Cuts at: 3811 4215 4729 4861 4867 4900 4930 4936 5041
Size: 404 514 132 6 33 30 6 105
Cuts at: 5041 5200 5221 5752 5818 6211 6268 6280 6400
Size: 159 21 531 66 393 57 12 120
Cuts at: 6400 6712 6733 6952 7389 8066 9214 9459 1606
Size: 312 21 219 437 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 531 514 498 437
404 393 360 312 288 245 228 219
159 150 132 120 105 66 60 57
33 30 26 21 21 12 6 6
NaeI GCC'GGC
Cuts at: 2759 6188 6620 2759
Size: 3429 432 6112
Fragments arranged by size:
6112 3429 432
NarI GG'CG_CC
Cuts at: 2761 5079 2761
Size: 2318 7655
NcoI C'CATG_G
Cuts at: 513 1065 3975 5322 513
Size: 552 2910 1347 5164
Fragments arranged by size:
5164 2910 1347 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9973
NgoMIV G'CCGG_C
Cuts at: 2757 6186 6618 2757
Size: 3429 432 6112
Fragments arranged by size:
6112 3429 432
NheI G'CTAG_C
Cuts at: 1052 5441 1052
Size: 4389 5584
NotI GC'GGCC_GC
Cuts at: 7420 7420
Size: 9973
NspI r_CATG'y
Cuts at: 1132 1229 2662 2716 6383 7057 7331 8876 1132
Size: 97 1433 54 3667 674 274 1545 2229
Fragments arranged by size:
3667 2229 1545 1433 674 274 97 54
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9973
PciI A'CATG_T
Cuts at: 7053 8872 7053
Size: 1819 8154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2497 2845 3166 3743 4156 6840 7246 1108
Size: 1389 348 321 577 413 2684 406 3835
Fragments arranged by size:
3835 2684 1389 577 413 406 348 321
PfoI T'CCnGG_A
Cuts at: 3490 3945 4074 5286 5880 6429 7239 3490
Size: 455 129 1212 594 549 810 6224
Fragments arranged by size:
6224 1212 810 594 549 455 129
PmeI GTTT'AAAC
Cuts at: 7277 7277
Size: 9973
PmlI CAC'GTG
Cuts at: 4228 4468 4228
Size: 240 9733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8042 8074 9555 9587 8042
Size: 32 1481 32 8428
Fragments arranged by size:
8428 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3646 3765 4993 6358 7109 3646
Size: 119 1228 1365 751 6510
Fragments arranged by size:
6510 1365 1228 751 119
PshAI GACnn'nnGTC
Cuts at: 1613 6841 1613
Size: 5228 4745
PsiI TTA'TAA
Cuts at: 7543 7543
Size: 9973
PspOMI G'GGCC_C
Cuts at: 2540 5016 2540
Size: 2476 7497
PspXI vC'TCGA_Gb
Cuts at: 4051 4051
Size: 9973
PstI C_TGCA'G
Cuts at: 830 2109 2184 2223 2652 3150 3282 3459 3810
Size: 1279 75 39 429 498 132 177 351
Cuts at: 3810 3870 4236 4578 4866 4908 4929 4935 5109
Size: 60 366 342 288 42 21 6 174
Cuts at: 5109 5187 5226 6183 6354 6732 6915 7182 7325
Size: 78 39 957 171 378 183 267 143
Cuts at: 7325 830
Size: 3478
Fragments arranged by size:
3478 1279 957 498 429 378 366 351
342 288 267 183 177 174 171 143
132 78 75 60 42 39 39 21
6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9973
PvuII CAG'CTG
Cuts at: 2104 2332 2842 3437 3523 3811 4729 4861 4900
Size: 228 510 595 86 288 918 132 39
Cuts at: 4900 4930 4936 5041 5200 5221 5818 6211 6400
Size: 30 6 105 159 21 597 393 189
Cuts at: 6400 6712 6733 2104
Size: 312 21 5344
Fragments arranged by size:
5344 918 597 595 510 393 312 288
228 189 159 132 105 86 39 30
21 21 6
SacI G_AGCT'C
Cuts at: 2085 3309 6033 6444 2085
Size: 1224 2724 411 5614
Fragments arranged by size:
5614 2724 1224 411
SacII CC_GC'GG
Cuts at: 4216 4216
Size: 9973
SalI G'TCGA_C
Cuts at: 1936 7315 1936
Size: 5379 4594
SanDI GG'GwC_CC
Cuts at: 7109 7109
Size: 9973
SapI GCTCTTCn'nnn_
Cuts at: 2021 3854 5132 5997 6402 2021
Size: 1833 1278 865 405 5592
Fragments arranged by size:
5592 1833 1278 865 405
SbfI CC_TGCA'GG
Cuts at: 7325 7325
Size: 9973
ScaI AGT'ACT
Cuts at: 8221 9933 8221
Size: 1712 8261
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 9973
SfcI C'TryA_G
Cuts at: 826 1045 2105 2180 2219 2377 2648 3146 3265
Size: 219 1060 75 39 158 271 498 119
Cuts at: 3265 3278 3455 3509 3806 3866 4178 4232 4574
Size: 13 177 54 297 60 312 54 342
Cuts at: 4574 4862 4904 4925 4931 5105 5183 5222 6179
Size: 288 42 21 6 174 78 39 957
Cuts at: 6179 6350 6728 6911 7178 7321 8456 9137 9328
Size: 171 378 183 267 143 1135 681 191
Cuts at: 9328 826
Size: 1471
Fragments arranged by size:
1471 1135 1060 957 681 498 378 342
312 297 288 271 267 219 191 183
177 174 171 158 143 119 78 75
60 54 54 42 39 39 21 13
6
SfoI GGC'GCC
Cuts at: 2762 5080 2762
Size: 2318 7655
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9973
SgrAI Cr'CCGG_yG
Cuts at: 1700 2757 6186 6417 1700
Size: 1057 3429 231 5256
Fragments arranged by size:
5256 3429 1057 231
SmaI CCC'GGG
Cuts at: 1872 2863 5084 7300 1872
Size: 991 2221 2216 4545
Fragments arranged by size:
4545 2221 2216 991
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 3176 3200 3650 4051 4771
Size: 197 926 1008 225 24 450 401 720
Cuts at: 4771 4817 5297 5357 6200 6443 6560 6869 6896
Size: 46 480 60 843 243 117 309 27
Cuts at: 6896 8077 8978 9240 820
Size: 1181 901 262 1553
Fragments arranged by size:
1553 1181 1008 926 901 843 720 480
450 401 309 262 243 225 197 117
60 46 27 24
SnaBI TAC'GTA
Cuts at: 493 7764 493
Size: 7271 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9973
SphI G_CATG'C
Cuts at: 1132 2716 6383 7331 1132
Size: 1584 3667 948 3774
Fragments arranged by size:
3774 3667 1584 948
SspI AAT'ATT
Cuts at: 5 52 7897 5
Size: 47 7845 2081
Fragments arranged by size:
7845 2081 47
StuI AGG'CCT
Cuts at: 1517 6093 1517
Size: 4576 5397
StyI C'CwwG_G
Cuts at: 513 1065 2553 2703 3642 3975 4188 5322 5430
Size: 552 1488 150 939 333 213 1134 108
Cuts at: 5430 5484 5550 5916 6669 6882 7412 513
Size: 54 66 366 753 213 530 3074
Fragments arranged by size:
3074 1488 1134 939 753 552 530 366
333 213 213 150 108 66 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 3497 4001 4766 8000 8017 8170
Size: 1255 730 1187 504 765 3234 17 153
Cuts at: 8170 8355 8800 325
Size: 185 445 1498
Fragments arranged by size:
3234 1498 1255 1187 765 730 504 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 2955 4354
Size: 274 80 33 51 1443 705 273 1399
Cuts at: 4354 5715 8219 9931 96
Size: 1361 2504 1712 138
Fragments arranged by size:
2504 1712 1443 1399 1361 705 274 273
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4664 4696 9933 9965 4664
Size: 32 5237 32 4672
Fragments arranged by size:
5237 4672 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9973
XbaI T'CTAG_A
Cuts at: 7309 7309
Size: 9973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2412 5923 7243 2412
Size: 3511 1320 5142
Fragments arranged by size:
5142 3511 1320
XhoI C'TCGA_G
Cuts at: 1943 4051 6443 6896 1943
Size: 2108 2392 453 5020
Fragments arranged by size:
5020 2392 2108 453
XmaI C'CCGG_G
Cuts at: 1870 2861 5082 7298 1870
Size: 991 2221 2216 4545
Fragments arranged by size:
4545 2221 2216 991
XmnI GAAnn'nnTTC
Cuts at: 7055 8102 7055
Size: 1047 8926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6607 7781 276
Size: 53 83 186 936 5073 1174 2468
Fragments arranged by size:
5073 2468 1174 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI PsrI RsrII SfiI SrfI SwaI