Restriction map for
pFN21AE2381
(Circular) (Six-base) MAPSORT of: pFN21AE2381.seq Check: 8988 from: 1 to: 9175
pFN21AE2381 9175 bp
With 182 enzymes: SgfI *
October 25, 2011 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5958 5958
Size: 9175
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5152 6985 278
Size: 53 83 186 936 3616 1833 2468
Fragments arranged by size:
3616 2468 1833 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4948 6518 1937
Size: 3011 1570 4594
Fragments arranged by size:
4594 3011 1570
Acc65I G'GTAC_C
Cuts at: 4704 6496 4704
Size: 1792 7383
AclI AA'CG_TT
Cuts at: 1563 3881 4738 7302 7675 1563
Size: 2318 857 2564 373 3063
Fragments arranged by size:
3063 2564 2318 857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 3414 4289 4683 5559 5949 6287 7238
Size: 962 820 875 394 876 390 338 951
Cuts at: 7238 8622 8890 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 962 951 876 875 820 394
390 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8978
AflIII A'CryG_T
Cuts at: 1203 3989 8074 9051 1203
Size: 2786 4085 977 1327
Fragments arranged by size:
4085 2786 1327 977
AgeI A'CCGG_T
Cuts at: 8006 8006
Size: 9175
AhdI GACnn_n'nnGTC
Cuts at: 7904 7904
Size: 9175
AleI CACnn'nnGTG
Cuts at: 1166 4356 1166
Size: 3190 5985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9175
AlwNI CAG_nnn'CTG
Cuts at: 3230 3383 5452 6112 6918 8490 3230
Size: 153 2069 660 806 1572 3915
Fragments arranged by size:
3915 2069 1572 806 660 153
ApaI G_GGCC'C
Cuts at: 5727 6389 5727
Size: 662 8513
ApaLI G'TGCA_C
Cuts at: 4490 5029 7232 8388 4490
Size: 539 2203 1156 5277
Fragments arranged by size:
5277 2203 1156 539
ApoI r'AATT_y
Cuts at: 1493 3424 3802 4113 4564 4958 6484 6710 6862
Size: 1931 378 311 451 394 1526 226 152
Cuts at: 6862 8971 1493
Size: 2109 1697
Fragments arranged by size:
2109 1931 1697 1526 451 394 378 311
226 152
AseI AT'TA_AT
Cuts at: 160 1032 7729 160
Size: 872 6697 1606
Fragments arranged by size:
6697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9175
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 2716 2770 2812 6500 1870
Size: 73 418 355 54 42 3688 4545
Fragments arranged by size:
4545 3688 418 355 73 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3825 3858 3825
Size: 33 9142
BamHI G'GATC_C
Cuts at: 6505 6872 6505
Size: 367 8808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4424 4704 6496 7951 618
Size: 325 217 627 2637 280 1792 1455 1842
Fragments arranged by size:
2637 1842 1792 1455 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2672 2885 4029 5727 5811 6389 6494 1391
Size: 1281 213 1144 1698 84 578 105 4072
Fragments arranged by size:
4072 1698 1281 1144 578 213 105 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2558 3839 4147 6390 928
Size: 971 659 1281 308 2243 3713
Fragments arranged by size:
3713 2243 1281 971 659 308
BbvCI CC'TCA_GC
Cuts at: 4086 5265 6273 4086
Size: 1179 1008 6988
Fragments arranged by size:
6988 1179 1008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2318 2352 7366 7400 1903
Size: 34 50 34 297 34 5014 34 3678
Fragments arranged by size:
5014 3678 297 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7067 8277 7067
Size: 1210 7965
BclI T'GATC_A
Cuts at: 1548 4845 1548
Size: 3297 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 7786 136
Size: 107 122 71 631 605 4050 2064 1525
Fragments arranged by size:
4050 2064 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2903 4039 9170 2903
Size: 1136 5131 2908
Fragments arranged by size:
5131 2908 1136
BlpI GC'TnA_GC
Cuts at: 4833 6592 4833
Size: 1759 7416
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2586 4494 5033 5482 5727 6179 6389
Size: 408 388 1908 539 449 245 452 210
Cuts at: 6389 7236 8392 1790
Size: 847 1156 2573
Fragments arranged by size:
2573 1908 1156 847 539 452 449 408
388 245 210
BmgBI CAC'GTC
Cuts at: 1318 3992 1318
Size: 2674 6501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3042 4168
Size: 416 53 83 399 24 1553 72 1126
Cuts at: 4168 5555 6357 6388 6906 7864 442
Size: 1387 802 31 518 958 1753
Fragments arranged by size:
1753 1553 1387 1126 958 802 518 416
399 83 72 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 5415 3215
Size: 2200 6975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2398 3936 4005 4044 4698 5079 5705 7835
Size: 1159 1538 69 39 654 381 626 2130
Cuts at: 7835 1239
Size: 2579
Fragments arranged by size:
2579 2130 1538 1159 654 626 381 69
39
Bpu10I CC'TnA_GC
Cuts at: 3014 3315 4086 5265 5390 5805 6232 6273 6361
Size: 301 771 1179 125 415 427 41 88
Cuts at: 6361 3014
Size: 5828
Fragments arranged by size:
5828 1179 771 427 415 301 125 88
41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3822 5665 5992 7300 8165 8463 3822
Size: 1843 327 1308 865 298 4534
Fragments arranged by size:
4534 1843 1308 865 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3030 3569 4099 5510 5968 6299 7838
Size: 617 1531 539 530 1411 458 331 1539
Cuts at: 7838 882
Size: 2219
Fragments arranged by size:
2219 1539 1531 1411 617 539 530 458
331
BsaAI yAC'GTr
Cuts at: 493 1204 6966 493
Size: 711 5762 2702
Fragments arranged by size:
5762 2702 711
BsaBI GATnn'nnATC
Cuts at: 9169 9169
Size: 9175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4945 5149 6982 7364
Size: 53 83 186 936 3412 204 1833 382
Cuts at: 7364 7999 275
Size: 635 1451
Fragments arranged by size:
3412 1833 1451 936 635 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 2439 2592 7608 8006 8280 8427 8838 2439
Size: 153 5016 398 274 147 411 2776
Fragments arranged by size:
5016 2776 411 398 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2561 2587 2591
Size: 30 165 30 834 30 294 26 4
Cuts at: 2591 2617 3619 3649 3871 3901 4245 4275 5411
Size: 26 1002 30 222 30 344 30 1136
Cuts at: 5411 5441 1178
Size: 30 4912
Fragments arranged by size:
4912 1136 1002 834 344 294 222 165
30 30 30 30 30 30 30 26
26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2064 2486 3548 5082 5546 5763 5768 6477
Size: 878 422 1062 1534 464 217 5 709
Cuts at: 6477 1186
Size: 3884
Fragments arranged by size:
3884 1534 1062 878 709 464 422 217
5
BseYI C'CCAG_C
Cuts at: 5254 5297 5499 5887 5922 6331 8378 8874 5254
Size: 43 202 388 35 409 2047 496 5555
Fragments arranged by size:
5555 2047 496 409 388 202 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 6209 1163
Size: 573 576 3897 4129
Fragments arranged by size:
4129 3897 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2323 2933 6625 7386 8414 1540
Size: 275 186 322 610 3692 761 1028 2301
Fragments arranged by size:
3692 2301 1028 761 610 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 4029 4494 5033 5964 7236 7321 8392 4029
Size: 465 539 931 1272 85 1071 4812
Fragments arranged by size:
4812 1272 1071 931 539 465 85
BsmI GAATG_Cn'
Cuts at: 6686 6779 6686
Size: 93 9082
BsmBI CGTCTCn'nnnn_
Cuts at: 2920 2920
Size: 9175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2586 2672 2885 4029 4494 5033
Size: 399 408 388 86 213 1144 465 539
Cuts at: 5033 5482 5727 5811 5964 6179 6389 6494 7236
Size: 449 245 84 153 215 210 105 742
Cuts at: 7236 7321 8392 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 539 465 449 408
399 388 245 215 213 210 153 105
86 85 84
BspEI T'CCGG_A
Cuts at: 8838 8838
Size: 9175
BspHI T'CATG_A
Cuts at: 4521 7064 8794 4521
Size: 2543 1730 4902
Fragments arranged by size:
4902 2543 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 5376 5958 6530 844
Size: 1644 2888 582 572 3489
Fragments arranged by size:
3489 2888 1644 582 572
BsrBI CCG'CTC
Cuts at: 1394 1823 7062 1394
Size: 429 5239 3507
Fragments arranged by size:
5239 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7670 7844 65
Size: 1183 6422 174 1396
Fragments arranged by size:
6422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7819 8006 1700
Size: 110 6009 187 2869
Fragments arranged by size:
6009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3817 96
Size: 3721 5454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9175
BssSI C'ACGA_G
Cuts at: 7235 8247 7235
Size: 1012 8163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3230 5555 794
Size: 2436 2325 4414
Fragments arranged by size:
4414 2436 2325
BstBI TT'CG_AA
Cuts at: 2465 7979 2465
Size: 5514 3661
BstEII G'GTnAC_C
Cuts at: 1184 2170 2874 1184
Size: 986 704 7485
Fragments arranged by size:
7485 986 704
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 4881 2161
Size: 2720 6455
BstYI r'GATC_y
Cuts at: 1972 2660 2903 3125 4039 5014 6505 6872 7257
Size: 688 243 222 914 975 1491 367 385
Cuts at: 7257 7274 8726 8812 8824 9170 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1491 1452 975 914 688 385 367
346 243 222 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5218 5218
Size: 9175
BtgI C'CryG_G
Cuts at: 513 1065 5520 513
Size: 552 4455 4168
Fragments arranged by size:
4455 4168 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8436
Fragments arranged by size:
8436 683 56
BtsI GCAGTG_nn'
Cuts at: 5361 6691 7485 7505 5361
Size: 1330 794 20 7031
Fragments arranged by size:
7031 1330 794 20
ClaI AT'CG_AT
Cuts at: 1554 2429 1554
Size: 875 8300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5692 5727 529
Size: 35 5128 35 3977
Fragments arranged by size:
5128 3977 35 35
DraI TTT'AAA
Cuts at: 6479 6479
Size: 9175
DraIII CAC_nnn'GTG
Cuts at: 6247 6247
Size: 9175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8182 809
Size: 507 6866 1802
Fragments arranged by size:
6866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 4219 5944
Size: 54 1425 50 275 18 901 1488 1725
Cuts at: 5944 6622 7511 9140 8
Size: 678 889 1629 43
Fragments arranged by size:
1725 1629 1488 1425 901 889 678 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6622 1537
Size: 275 4810 4090
Fragments arranged by size:
4810 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3116 3594 3687 3954 4659 5172 5789 6448
Size: 1763 478 93 267 705 513 617 659
Cuts at: 6448 7105 1353
Size: 657 3423
Fragments arranged by size:
3423 1763 705 659 657 617 513 478
267 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7762 8136 8282 387
Size: 1064 138 6173 374 146 1280
Fragments arranged by size:
6173 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4027 4027
Size: 9175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2398 2594 3414 3936 4005 4044 4289
Size: 393 766 196 820 522 69 39 245
Cuts at: 4289 4683 4698 5079 5559 5705 5949 6287 7238
Size: 394 15 381 480 146 244 338 951
Cuts at: 7238 7835 8622 8890 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 951 820 787 766 597 522 480
394 393 381 338 268 245 244 196
146 69 39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2815 2890 2920 6039 2815
Size: 75 30 3119 5951
Fragments arranged by size:
5951 3119 75 30
EcoO109I rG'GnC_Cy
Cuts at: 5723 5724 5799 6126 6208 6226 6385 6425 5723
Size: 1 75 327 82 18 159 40 8473
Fragments arranged by size:
8473 327 159 82 75 40 18 1
EcoRI G'AATT_C
Cuts at: 4958 6484 6862 4958
Size: 1526 378 7271
Fragments arranged by size:
7271 1526 378
EcoRV GAT'ATC
Cuts at: 2230 2445 2230
Size: 215 8960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2973 3965 5876 2973
Size: 992 1911 6272
Fragments arranged by size:
6272 1911 992
FspI TGC'GCA
Cuts at: 7681 7681
Size: 9175
HaeII r_GCGC'y
Cuts at: 1678 5871 8322 1678
Size: 4193 2451 2531
Fragments arranged by size:
4193 2531 2451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2237 2251 2269 2429
Size: 32 135 32 647 18 14 18 160
Cuts at: 2429 2461 2561 2587 2593 2619 2699 2731 3022
Size: 32 100 26 6 26 80 32 291
Cuts at: 3022 3054 3446 3478 3529 3561 3976 4008 4999
Size: 32 392 32 51 32 415 32 991
Cuts at: 4999 5031 7816 7848 7890 7922 1373
Size: 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 991 647 415 392 291 160
135 100 80 51 42 32 32 32
32 32 32 32 32 32 32 32
26 26 18 18 14 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4366 6519 6765 669
Size: 988 281 2428 2153 246 3079
Fragments arranged by size:
3079 2428 2153 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5598 748
Size: 4850 4325
HpaI GTT'AAC
Cuts at: 4366 6765 4366
Size: 2399 6776
KpnI G_GTAC'C
Cuts at: 4708 6500 4708
Size: 1792 7383
MfeI C'AATT_G
Cuts at: 3597 6774 3597
Size: 3177 5998
MluI A'CGCG_T
Cuts at: 9051 9051
Size: 9175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 3014 3432 5454 8289 8473 1305
Size: 963 746 418 2022 2835 184 2007
Fragments arranged by size:
2835 2022 2007 963 746 418 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2733 4221 5946 10
Size: 54 1425 343 901 1488 1725 3239
Fragments arranged by size:
3239 1725 1488 1425 901 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4356 7133 7492 7651 518
Size: 648 3190 2777 359 159 2042
Fragments arranged by size:
3190 2777 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2964 3227 3854 4877 5036 5335 5413 5452
Size: 1358 263 627 1023 159 299 78 39
Cuts at: 5452 5615 5653 6591 7268 8416 8661 1606
Size: 163 38 938 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 1023 938 677 627 299
263 245 163 159 78 39 38
NcoI C'CATG_G
Cuts at: 513 1065 5520 513
Size: 552 4455 4168
Fragments arranged by size:
4455 4168 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9175
NotI GC'GGCC_GC
Cuts at: 6622 6622
Size: 9175
NspI r_CATG'y
Cuts at: 1132 1229 5005 5623 5769 6302 6533 8078 1132
Size: 97 3776 618 146 533 231 1545 2229
Fragments arranged by size:
3776 2229 1545 618 533 231 146 97
PasI CC'CwG_GG
Cuts at: 1348 2191 1348
Size: 843 8332
PciI A'CATG_T
Cuts at: 8074 8074
Size: 9175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6112 1108
Size: 5004 4171
PfoI T'CCnGG_A
Cuts at: 2663 2800 2663
Size: 137 9038
PmeI GTTT'AAAC
Cuts at: 6479 6479
Size: 9175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3147 7244 7276 8757 8789 3115
Size: 32 4097 32 1481 32 3501
Fragments arranged by size:
4097 3501 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6126 6208 6226 6126
Size: 82 18 9075
Fragments arranged by size:
9075 82 18
PshAI GACnn'nnGTC
Cuts at: 1613 2298 2748 2932 1613
Size: 685 450 184 7856
Fragments arranged by size:
7856 685 450 184
PsiI TTA'TAA
Cuts at: 6745 6745
Size: 9175
PspOMI G'GGCC_C
Cuts at: 5723 6385 5723
Size: 662 8513
PspXI vC'TCGA_Gb
Cuts at: 2770 2770
Size: 9175
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5031 5063 5031
Size: 32 9143
PstI C_TGCA'G
Cuts at: 830 3241 4981 5376 6527 830
Size: 2411 1740 395 1151 3478
Fragments arranged by size:
3478 2411 1740 1151 395
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9175
PvuII CAG'CTG
Cuts at: 3227 3854 4877 5036 5335 5413 5452 5615 5653
Size: 627 1023 159 299 78 39 163 38
Cuts at: 5653 3227
Size: 6749
Fragments arranged by size:
6749 1023 627 299 163 159 78 39
38
SacI G_AGCT'C
Cuts at: 4029 4029
Size: 9175
SalI G'TCGA_C
Cuts at: 1936 6517 1936
Size: 4581 4594
SanDI GG'GwC_CC
Cuts at: 6226 6226
Size: 9175
SapI GCTCTTCn'nnn_
Cuts at: 3687 5172 3687
Size: 1485 7690
SbfI CC_TGCA'GG
Cuts at: 6527 6527
Size: 9175
ScaI AGT'ACT
Cuts at: 3745 5230 5987 7423 9135 3745
Size: 1485 757 1436 1712 3785
Fragments arranged by size:
3785 1712 1485 1436 757
SfcI C'TryA_G
Cuts at: 826 1045 2887 2917 3237 4977 5048 5372 6523
Size: 219 1842 30 320 1740 71 324 1151
Cuts at: 6523 7658 8339 8530 826
Size: 1135 681 191 1471
Fragments arranged by size:
1842 1740 1471 1151 1135 681 324 320
219 191 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 5722 5722
Size: 9175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9175
SmaI CCC'GGG
Cuts at: 1872 2363 6502 1872
Size: 491 4139 4545
Fragments arranged by size:
4545 4139 491
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2770 2812 3837 5644 5971 7279
Size: 197 926 827 42 1025 1807 327 1308
Cuts at: 7279 8180 8442 820
Size: 901 262 1553
Fragments arranged by size:
1807 1553 1308 1025 926 901 827 327
262 197 42
SnaBI TAC'GTA
Cuts at: 493 6966 493
Size: 6473 2702
SpeI A'CTAG_T
Cuts at: 152 4258 152
Size: 4106 5069
SphI G_CATG'C
Cuts at: 1132 6533 1132
Size: 5401 3774
SspI AAT'ATT
Cuts at: 5 52 7099 5
Size: 47 7047 2081
Fragments arranged by size:
7047 2081 47
StuI AGG'CCT
Cuts at: 1517 5313 5969 6372 1517
Size: 3796 656 403 4320
Fragments arranged by size:
4320 3796 656 403
StyI C'CwwG_G
Cuts at: 513 1065 3264 4236 5520 6099 6151 6614 513
Size: 552 2199 972 1284 579 52 463 3074
Fragments arranged by size:
3074 2199 1284 972 579 552 463 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2295 2402 2638 2815 2989 7202 7219
Size: 1255 715 107 236 177 174 4213 17
Cuts at: 7219 7372 7557 8002 325
Size: 153 185 445 1498
Fragments arranged by size:
4213 1498 1255 715 445 236 185 177
174 153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 3817 5228
Size: 274 80 33 51 1443 1766 74 1411
Cuts at: 5228 5985 7421 9133 96
Size: 757 1436 1712 138
Fragments arranged by size:
1766 1712 1443 1436 1411 757 274 138
80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 3994 4026 4156 4188 4245 4277 4571
Size: 32 1620 32 130 32 57 32 294
Cuts at: 4571 4603 9135 9167 2342
Size: 32 4532 32 2350
Fragments arranged by size:
4532 2350 1620 294 130 57 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6000 1316
Size: 4684 4491
XbaI T'CTAG_A
Cuts at: 6511 6511
Size: 9175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4825 4825
Size: 9175
XhoI C'TCGA_G
Cuts at: 1943 2770 2812 1943
Size: 827 42 8306
Fragments arranged by size:
8306 827 42
XmaI C'CCGG_G
Cuts at: 1870 2361 6500 1870
Size: 491 4139 4545
Fragments arranged by size:
4545 4139 491
XmnI GAAnn'nnTTC
Cuts at: 3948 7304 3948
Size: 3356 5819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5150 6983 276
Size: 53 83 186 936 3616 1833 2468
Fragments arranged by size:
3616 2468 1833 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI RsrII SacII SexAI SfoI SrfI SwaI