Restriction map for
pFN21AE2379
(Circular) (Six-base) MAPSORT of: pFN21AE2379.seq Check: 6872 from: 1 to: 8182
pFN21AE2379 8182 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2207 2207
Size: 8182
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4386 5992 278
Size: 53 83 186 936 2850 1606 2468
Fragments arranged by size:
2850 2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5525 1937
Size: 3588 4594
Acc65I G'GTAC_C
Cuts at: 5503 5503
Size: 8182
AclI AA'CG_TT
Cuts at: 1563 6309 6682 1563
Size: 4746 373 3063
Fragments arranged by size:
4746 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 3323 3596 3844 4175 4630 6245 7629
Size: 887 804 273 248 331 455 1615 1384
Cuts at: 7629 7897 1632
Size: 268 1917
Fragments arranged by size:
1917 1615 1384 887 804 455 331 273
268 248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7985
AflIII A'CryG_T
Cuts at: 1203 3978 4533 7081 8058 1203
Size: 2775 555 2548 977 1327
Fragments arranged by size:
2775 2548 1327 977 555
AgeI A'CCGG_T
Cuts at: 7013 7013
Size: 8182
AhdI GACnn_n'nnGTC
Cuts at: 6911 6911
Size: 8182
AleI CACnn'nnGTG
Cuts at: 1166 3875 4310 1166
Size: 2709 435 5038
Fragments arranged by size:
5038 2709 435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2963 1184
Size: 1779 6403
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 8150
AlwNI CAG_nnn'CTG
Cuts at: 2260 3136 3358 4000 4036 5004 5452 5925 7497
Size: 876 222 642 36 968 448 473 1572
Cuts at: 7497 2260
Size: 2945
Fragments arranged by size:
2945 1572 968 876 642 473 448 222
36
ApaI G_GGCC'C
Cuts at: 2453 2685 4264 4477 4841 5377 5437 2453
Size: 232 1579 213 364 536 60 5198
Fragments arranged by size:
5198 1579 536 364 232 213 60
ApaLI G'TGCA_C
Cuts at: 3667 6239 7395 3667
Size: 2572 1156 4454
Fragments arranged by size:
4454 2572 1156
ApoI r'AATT_y
Cuts at: 1493 5491 5717 5869 7978 1493
Size: 3998 226 152 2109 1697
Fragments arranged by size:
3998 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6736 160
Size: 872 5704 1606
Fragments arranged by size:
5704 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2529 2575 2678 2757 2785 2820
Size: 73 163 423 46 103 79 28 35
Cuts at: 2820 4006 4094 4353 4609 5092 5106 5254 5368
Size: 1186 88 259 256 483 14 148 114
Cuts at: 5368 5473 5507 1870
Size: 105 34 4545
Fragments arranged by size:
4545 1186 483 423 259 256 163 148
114 105 103 88 79 73 46 35
34 28 14
BamHI G'GATC_C
Cuts at: 5512 5879 5512
Size: 367 7815
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2267 2341 2570 2614 3146
Size: 325 217 627 480 74 229 44 532
Cuts at: 3146 3178 4870 4970 5503 6958 618
Size: 32 1692 100 533 1455 1842
Fragments arranged by size:
1842 1692 1455 627 533 532 480 325
229 217 100 74 44 32
BanII G_rGCy'C
Cuts at: 1391 2453 2679 2685 2792 4264 4361 4477 4616
Size: 1062 226 6 107 1472 97 116 139
Cuts at: 4616 4796 4841 4920 5040 5377 5437 5501 1391
Size: 180 45 79 120 337 60 64 4072
Fragments arranged by size:
4072 1472 1062 337 226 180 139 120
116 107 97 79 64 60 45 6
BbeI G_GCGC'C
Cuts at: 2271 2574 3150 4974 2271
Size: 303 576 1824 5479
Fragments arranged by size:
5479 1824 576 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3265 3584 928
Size: 971 1366 319 5526
Fragments arranged by size:
5526 1366 971 319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2811 2845 6373 6407 1903
Size: 34 50 34 790 34 3528 34 3678
Fragments arranged by size:
3678 3528 790 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6074 7284 6074
Size: 1210 6972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2579 2855 3462
Size: 107 122 71 631 605 907 276 607
Cuts at: 3462 3813 4777 6793 136
Size: 351 964 2016 1525
Fragments arranged by size:
2016 1525 964 907 631 607 605 351
276 122 107 71
BglII A'GATC_T
Cuts at: 3918 8177 3918
Size: 4259 3923
BlpI GC'TnA_GC
Cuts at: 5599 5599
Size: 8182
Bme1580I G_kGCm'C
Cuts at: 1790 2346 2453 2685 3671 4013 4264 4477 4841
Size: 556 107 232 986 342 251 213 364
Cuts at: 4841 4875 5377 5437 6243 7399 1790
Size: 34 502 60 806 1156 2573
Fragments arranged by size:
2573 1156 986 806 556 502 364 342
251 232 213 107 60 34
BmgBI CAC'GTC
Cuts at: 1318 2026 2596 3154 5116 1318
Size: 708 570 558 1962 4384
Fragments arranged by size:
4384 1962 708 570 558
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5335 5913 6871
Size: 416 53 83 399 24 3918 578 958
Cuts at: 6871 442
Size: 1753
Fragments arranged by size:
3918 1753 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3698 3966 1056
Size: 2642 268 5272
Fragments arranged by size:
5272 2642 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2776 4541 4592 4795 4837 4864 5071 5123
Size: 1537 1765 51 203 42 27 207 52
Cuts at: 5123 5180 6842 1239
Size: 57 1662 2579
Fragments arranged by size:
2579 1765 1662 1537 207 203 57 52
51 42 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 6307 7172 7470 3017
Size: 3290 865 298 3729
Fragments arranged by size:
3729 3290 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 6845 882
Size: 617 3657 1689 2219
Fragments arranged by size:
3657 2219 1689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2668 5973 493
Size: 711 1464 3305 2702
Fragments arranged by size:
3305 2702 1464 711
BsaBI GATnn'nnATC
Cuts at: 8176 8176
Size: 8182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2571 2606 2781
Size: 53 83 186 936 735 303 35 175
Cuts at: 2781 3147 4383 4971 5407 5989 6371 7006 275
Size: 366 1236 588 436 582 382 635 1451
Fragments arranged by size:
1451 1236 936 735 635 588 582 436
382 366 303 186 175 83 53 35
BsaWI w'CCGG_w
Cuts at: 2301 6615 7013 7287 7434 7845 2301
Size: 4314 398 274 147 411 2638
Fragments arranged by size:
4314 2638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4334 4364 4781 4811 1178
Size: 30 165 30 2931 30 417 30 4549
Fragments arranged by size:
4549 2931 417 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2705 3458 3572 4112 5060 5372 1186
Size: 1102 417 753 114 540 948 312 3996
Fragments arranged by size:
3996 1102 948 753 540 417 312 114
BseYI C'CCAG_C
Cuts at: 2263 2618 4319 5063 5428 5445 7385 7881 2263
Size: 355 1701 744 365 17 1940 496 2564
Fragments arranged by size:
2564 1940 1701 744 496 365 355 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2179 2195 3746 4178 5196 5438 1163
Size: 573 443 16 1551 432 1018 242 3907
Fragments arranged by size:
3907 1551 1018 573 443 432 242 16
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2549 2859 3466 3648 4772 5632
Size: 275 186 548 310 607 182 1124 860
Cuts at: 5632 6393 7421 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1124 1028 860 761 607 548 310
275 186 182
BsiHKAI G_wGCw'C
Cuts at: 3671 5040 6243 6328 7399 3671
Size: 1369 1203 85 1071 4454
Fragments arranged by size:
4454 1369 1203 1071 85
BsmI GAATG_Cn'
Cuts at: 5693 5786 5693
Size: 93 8089
BsmBI CGTCTCn'nnnn_
Cuts at: 3302 3898 3302
Size: 596 7586
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2453 2679 2685 2792 3671 4013
Size: 399 556 107 226 6 107 879 342
Cuts at: 4013 4264 4361 4477 4616 4796 4841 4875 4920
Size: 251 97 116 139 180 45 34 45
Cuts at: 4920 5040 5377 5437 5501 6243 6328 7399 1391
Size: 120 337 60 64 742 85 1071 2174
Fragments arranged by size:
2174 1071 879 742 556 399 342 337
251 226 180 139 120 116 107 107
97 85 64 60 45 45 34 6
BspEI T'CCGG_A
Cuts at: 7845 7845
Size: 8182
BspHI T'CATG_A
Cuts at: 2431 6071 7801 2431
Size: 3640 1730 2812
Fragments arranged by size:
3640 2812 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 3540 4335 4524 4596 5537 844
Size: 1363 1333 795 189 72 941 3489
Fragments arranged by size:
3489 1363 1333 941 795 189 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2695 4206 6069 1394
Size: 429 872 1511 1863 3507
Fragments arranged by size:
3507 1863 1511 872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6677 6851 65
Size: 1183 5429 174 1396
Fragments arranged by size:
5429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 4767 6826 7013 1700
Size: 110 198 2759 2059 187 2869
Fragments arranged by size:
2869 2759 2059 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8182
BssHII G'CGCG_C
Cuts at: 1925 2733 2878 1925
Size: 808 145 7229
Fragments arranged by size:
7229 808 145
BssSI C'ACGA_G
Cuts at: 2975 6242 7254 2975
Size: 3267 1012 3903
Fragments arranged by size:
3903 3267 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 2935 794
Size: 1466 675 6041
Fragments arranged by size:
6041 1466 675
BstBI TT'CG_AA
Cuts at: 6986 6986
Size: 8182
BstEII G'GTnAC_C
Cuts at: 1184 3124 1184
Size: 1940 6242
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2467
Size: 8182
BstYI r'GATC_y
Cuts at: 1972 3918 4068 4368 4500 5512 5879 6264 6281
Size: 1946 150 300 132 1012 367 385 17
Cuts at: 6281 7733 7819 7831 8177 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1946 1452 1012 385 367 346 300
150 132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2583 2610 2655 2685 4781 513
Size: 552 1518 27 45 30 2096 3914
Fragments arranged by size:
3914 2096 1518 552 45 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 2736 3454 3667 487
Size: 683 56 854 656 718 213 5002
Fragments arranged by size:
5002 854 718 683 656 213 56
BtsI GCAGTG_nn'
Cuts at: 2258 2933 4208 4219 4831 5485 5698 6492 6512
Size: 675 1275 11 612 654 213 794 20
Cuts at: 6512 2258
Size: 3928
Fragments arranged by size:
3928 1275 794 675 654 612 213 20
11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8147
DraI TTT'AAA
Cuts at: 5486 5486
Size: 8182
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7189 809
Size: 507 5873 1802
Fragments arranged by size:
5873 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 2856 3463
Size: 54 1425 50 275 18 910 116 607
Cuts at: 3463 3513 3645 3984 4266 4392 4769 5184 5629
Size: 50 132 339 282 126 377 415 445
Cuts at: 5629 6518 8147 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 607 445 415 377
339 282 275 132 126 116 54 50
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2856 3463 3645 4769 5629 1537
Size: 275 1044 607 182 1124 860 4090
Fragments arranged by size:
4090 1124 1044 860 607 275 182
EarI CTCTTCn'nnn_
Cuts at: 1353 4934 6112 1353
Size: 3581 1178 3423
Fragments arranged by size:
3581 3423 1178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 2712 4853 6769 7143 7289
Size: 1064 138 894 229 2141 1916 374 146
Cuts at: 7289 387
Size: 1280
Fragments arranged by size:
2141 1916 1280 1064 894 374 229 146
138
EcoICRI GAG'CTC
Cuts at: 5038 5038
Size: 8182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2776 3323 3596 3844 4175 4541
Size: 393 887 257 547 273 248 331 366
Cuts at: 4541 4592 4630 4795 4837 4864 5071 5123 5180
Size: 51 38 165 42 27 207 52 57
Cuts at: 5180 6245 6842 7629 7897 1239
Size: 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 887 787 597 547 393 366
331 273 268 257 248 207 165 57
52 51 42 38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3302 4520 4678 4925 3302
Size: 1218 158 247 6559
Fragments arranged by size:
6559 1218 247 158
EcoO109I rG'GnC_Cy
Cuts at: 2421 2449 2450 2711 3133 4261 4324 4474 4837
Size: 28 1 261 422 1128 63 150 363
Cuts at: 4837 4890 5258 5373 5374 5396 5433 5434 2421
Size: 53 368 115 1 22 37 1 5169
Fragments arranged by size:
5169 1128 422 368 363 261 150 115
63 53 37 28 22 1 1 1
EcoRI G'AATT_C
Cuts at: 5491 5869 5491
Size: 378 7804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4690 4690
Size: 8182
FseI GG_CCGG'CC
Cuts at: 4771 4771
Size: 8182
FspI TGC'GCA
Cuts at: 2939 6688 2939
Size: 3749 4433
HaeII r_GCGC'y
Cuts at: 1678 2233 2271 2522 2574 3150 3848 4093 4974
Size: 555 38 251 52 576 698 245 881
Cuts at: 4974 5146 7329 1678
Size: 172 2183 2531
Fragments arranged by size:
2531 2183 881 698 576 555 251 245
172 52 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3614 3646 4060 4092 4781
Size: 32 135 32 2042 32 414 32 689
Cuts at: 4781 4813 6823 6855 6897 6929 1373
Size: 32 2010 32 42 32 2626
Fragments arranged by size:
2626 2042 2010 689 414 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5526 5772 669
Size: 988 281 3588 246 3079
Fragments arranged by size:
3588 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8182
HpaI GTT'AAC
Cuts at: 5772 5772
Size: 8182
KasI G'GCGC_C
Cuts at: 2267 2570 3146 4970 2267
Size: 303 576 1824 5479
Fragments arranged by size:
5479 1824 576 303
KpnI G_GTAC'C
Cuts at: 5507 5507
Size: 8182
MfeI C'AATT_G
Cuts at: 5781 5781
Size: 8182
MluI A'CGCG_T
Cuts at: 8058 8058
Size: 8182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 4575 5013 7296 7480 1305
Size: 1689 1581 438 2283 184 2007
Fragments arranged by size:
2283 2007 1689 1581 438 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4268 4394 5186 10
Size: 54 1425 343 2436 126 792 3006
Fragments arranged by size:
3006 2436 1425 792 343 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3506 3875 4031 4139 4310 6140
Size: 648 843 1497 369 156 108 171 1830
Cuts at: 6140 6499 6658 518
Size: 359 159 2042
Fragments arranged by size:
2042 1830 1497 843 648 369 359 171
159 156 108
MspA1I CmG'CkG
Cuts at: 1606 2463 2612 2687 2796 2943 3195 3358 3804
Size: 857 149 75 109 147 252 163 446
Cuts at: 3804 3997 5200 5284 5428 5598 6275 7423 7668
Size: 193 1203 84 144 170 677 1148 245
Cuts at: 7668 1606
Size: 2120
Fragments arranged by size:
2120 1203 1148 857 677 446 252 245
193 170 163 149 147 144 109 84
75
NaeI GCC'GGC
Cuts at: 4769 4769
Size: 8182
NarI GG'CG_CC
Cuts at: 2268 2571 3147 4971 2268
Size: 303 576 1824 5479
Fragments arranged by size:
5479 1824 576 303
NcoI C'CATG_G
Cuts at: 513 1065 2655 513
Size: 552 1590 6040
Fragments arranged by size:
6040 1590 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8182
NgoMIV G'CCGG_C
Cuts at: 4767 4767
Size: 8182
NheI G'CTAG_C
Cuts at: 1052 3694 3962 1052
Size: 2642 268 5272
Fragments arranged by size:
5272 2642 268
NotI GC'GGCC_GC
Cuts at: 5629 5629
Size: 8182
NspI r_CATG'y
Cuts at: 1132 1229 4292 4319 4537 5540 7085 1132
Size: 97 3063 27 218 1003 1545 2229
Fragments arranged by size:
3063 2229 1545 1003 218 97 27
PasI CC'CwG_GG
Cuts at: 1348 3142 1348
Size: 1794 6388
PciI A'CATG_T
Cuts at: 4533 7081 4533
Size: 2548 5634
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2463 3358 5347 1108
Size: 1355 895 1989 3943
Fragments arranged by size:
3943 1989 1355 895
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 8182
PmeI GTTT'AAAC
Cuts at: 5486 5486
Size: 8182
PmlI CAC'GTG
Cuts at: 2668 2668
Size: 8182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6251 6283 7764 7796 6251
Size: 32 1481 32 6637
Fragments arranged by size:
6637 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2711 3133 4324 2421
Size: 290 422 1191 6279
Fragments arranged by size:
6279 1191 422 290
PshAI GACnn'nnGTC
Cuts at: 1613 5222 1613
Size: 3609 4573
PsiI TTA'TAA
Cuts at: 5752 5752
Size: 8182
PspOMI G'GGCC_C
Cuts at: 2449 2681 4260 4473 4837 5373 5433 2449
Size: 232 1579 213 364 536 60 5198
Fragments arranged by size:
5198 1579 536 364 232 213 60
PspXI vC'TCGA_Gb
Cuts at: 4094 4094
Size: 8182
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4529 4561 4529
Size: 32 8150
PstI C_TGCA'G
Cuts at: 830 2801 3177 4179 4218 5199 5534 830
Size: 1971 376 1002 39 981 335 3478
Fragments arranged by size:
3478 1971 1002 981 376 335 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8182
PvuII CAG'CTG
Cuts at: 2463 2796 5200 5428 2463
Size: 333 2404 228 5217
Fragments arranged by size:
5217 2404 333 228
SacI G_AGCT'C
Cuts at: 5040 5040
Size: 8182
SacII CC_GC'GG
Cuts at: 2613 2688 2613
Size: 75 8107
SalI G'TCGA_C
Cuts at: 1936 5524 1936
Size: 3588 4594
SanDI GG'GwC_CC
Cuts at: 2421 2711 2421
Size: 290 7892
SapI GCTCTTCn'nnn_
Cuts at: 4934 4934
Size: 8182
SbfI CC_TGCA'GG
Cuts at: 4179 5534 4179
Size: 1355 6827
ScaI AGT'ACT
Cuts at: 4103 6430 8142 4103
Size: 2327 1712 4143
Fragments arranged by size:
4143 2327 1712
SfcI C'TryA_G
Cuts at: 826 1045 2797 3173 4175 4214 5195 5204 5236
Size: 219 1752 376 1002 39 981 9 32
Cuts at: 5236 5530 6665 7346 7537 826
Size: 294 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 1002 981 681 376 294
219 191 39 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3813 4777 3813
Size: 964 7218
SfoI GGC'GCC
Cuts at: 2269 2572 3148 4972 2269
Size: 303 576 1824 5479
Fragments arranged by size:
5479 1824 576 303
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8182
SmaI CCC'GGG
Cuts at: 1872 2531 2577 2759 4355 4611 5094 5108 5256
Size: 659 46 182 1596 256 483 14 148
Cuts at: 5256 5370 5475 5509 1872
Size: 114 105 34 4545
Fragments arranged by size:
4545 1596 659 483 256 182 148 114
105 46 34 14
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 4094 6286 7187 7449 820
Size: 197 926 1089 1062 2192 901 262 1553
Fragments arranged by size:
2192 1553 1089 1062 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5973 493
Size: 5480 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8182
SphI G_CATG'C
Cuts at: 1132 4319 5540 1132
Size: 3187 1221 3774
Fragments arranged by size:
3774 3187 1221
SspI AAT'ATT
Cuts at: 5 52 6106 5
Size: 47 6054 2081
Fragments arranged by size:
6054 2081 47
StuI AGG'CCT
Cuts at: 1517 4447 1517
Size: 2930 5252
StyI C'CwwG_G
Cuts at: 513 1065 2655 4245 4725 5316 5621 513
Size: 552 1590 1590 480 591 305 3074
Fragments arranged by size:
3074 1590 1590 591 552 480 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2632 2946 3143 6209 6226 6379 6564
Size: 1255 1052 314 197 3066 17 153 185
Cuts at: 6564 7009 325
Size: 445 1498
Fragments arranged by size:
3066 1498 1255 1052 445 314 197 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3165 4002 4101
Size: 274 80 33 51 1443 1188 837 99
Cuts at: 4101 4341 6428 8140 96
Size: 240 2087 1712 138
Fragments arranged by size:
2087 1712 1443 1188 837 274 240 138
99 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3171 3203 4103 4135 4547 4579 8142 8174 3171
Size: 32 900 32 412 32 3563 32 3179
Fragments arranged by size:
3563 3179 900 412 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4385 1316
Size: 3069 5113
XbaI T'CTAG_A
Cuts at: 5518 5518
Size: 8182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2464 2800 3445 3721 5314 2107
Size: 357 336 645 276 1593 4975
Fragments arranged by size:
4975 1593 645 357 336 276
XhoI C'TCGA_G
Cuts at: 1943 4094 1943
Size: 2151 6031
XmaI C'CCGG_G
Cuts at: 1870 2529 2575 2757 4353 4609 5092 5106 5254
Size: 659 46 182 1596 256 483 14 148
Cuts at: 5254 5368 5473 5507 1870
Size: 114 105 34 4545
Fragments arranged by size:
4545 1596 659 483 256 182 148 114
105 46 34 14
XmnI GAAnn'nnTTC
Cuts at: 2042 3920 4160 6311 2042
Size: 1878 240 2151 3913
Fragments arranged by size:
3913 2151 1878 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4384 5990 276
Size: 53 83 186 936 2850 1606 2468
Fragments arranged by size:
2850 2468 1606 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI
PacI RsrII SexAI SrfI SwaI