Restriction map for
pFN21AE2376
(Circular) (Six-base) MAPSORT of: pFN21AE2376.seq Check: 6852 from: 1 to: 6256
pFN21AE2376 6256 bp
With 182 enzymes: SgfI *
September 20, 2011 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2700 3599 1937
Size: 763 899 4594
Fragments arranged by size:
4594 899 763
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3046 3384 4319 5703 5971 1632
Size: 1414 338 935 1384 268 1917
Fragments arranged by size:
1917 1414 1384 935 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 2860 5155 6132 1203
Size: 1657 2295 977 1327
Fragments arranged by size:
2295 1657 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3999 5571 3999
Size: 1572 4684
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2111 2264 3581 1870
Size: 73 134 34 153 1317 4545
Fragments arranged by size:
4545 1317 153 134 73 34
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 3577 5032 618
Size: 325 217 627 645 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 645 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2060 2109 2264 2429 2487 3575 1391
Size: 655 14 49 155 165 58 1088 4072
Fragments arranged by size:
4072 1088 655 165 155 58 49 14
BbeI G_GCGC'C
Cuts at: 2436 2436
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 3372 928
Size: 971 402 1071 3812
Fragments arranged by size:
3812 1071 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3219 3253 4447 4481 1903
Size: 34 1282 34 1194 34 3678
Fragments arranged by size:
3678 1282 1194 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2279 2382 2511
Size: 107 122 71 631 605 607 103 129
Cuts at: 2511 2616 4867 136
Size: 105 2251 1525
Fragments arranged by size:
2251 1525 631 607 605 129 122 107
105 103 71
BglII A'GATC_T
Cuts at: 2833 6251 2833
Size: 3418 2838
BlpI GC'TnA_GC
Cuts at: 2103 3673 2103
Size: 1570 4686
Bme1580I G_kGCm'C
Cuts at: 1790 4317 5473 1790
Size: 2527 1156 2573
Fragments arranged by size:
2573 2527 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3987 4945
Size: 416 53 83 399 24 1169 1401 958
Cuts at: 4945 442
Size: 1753
Fragments arranged by size:
1753 1401 1169 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3041 1056
Size: 1985 4271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2434 2062
Size: 372 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2498 2533 2715 4916 1239
Size: 818 441 35 182 2201 2579
Fragments arranged by size:
2579 2201 818 441 182 35
Bpu10I CC'TnA_GC
Cuts at: 2054 2651 2054
Size: 597 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 4919 882
Size: 617 906 2514 2219
Fragments arranged by size:
2514 2219 906 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 4063 4445 5080
Size: 53 83 186 936 900 1630 382 635
Cuts at: 5080 275
Size: 1451
Fragments arranged by size:
1630 1451 936 900 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2618 2648 1178
Size: 30 165 30 1215 30 4786
Fragments arranged by size:
4786 1215 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2129 2132 2135 2144 2147 2150 2159
Size: 868 75 3 3 9 3 3 9
Cuts at: 2159 2235 2315 2318 2330 2333 2369 2372 2495
Size: 76 80 3 12 3 36 3 123
Cuts at: 2495 2892 3067 1186
Size: 397 175 4375
Fragments arranged by size:
4375 868 397 175 123 80 76 75
36 12 9 9 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5683
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 4317 4402 5473 2046
Size: 2271 85 1071 2829
Fragments arranged by size:
2829 2271 1071 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2237
Size: 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2060 2109 2264 2429 2487 3575
Size: 399 256 14 49 155 165 58 1088
Cuts at: 3575 4317 4402 5473 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1088 1071 742 399 256 165 155
85 58 49 14
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3611 844
Size: 2767 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2418 4143 1394
Size: 429 595 1725 3507
Fragments arranged by size:
3507 1725 595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 3016 4751 4925 65
Size: 1183 1494 274 1735 174 1396
Fragments arranged by size:
1735 1494 1396 1183 274 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 4900 5087 1700
Size: 110 800 2290 187 2869
Fragments arranged by size:
2869 2290 800 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2833 3586 3953 4338 4355 5807 5893 5905
Size: 861 753 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 861 753 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2495 3179 513
Size: 552 939 491 684 3590
Fragments arranged by size:
3590 939 684 552 491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3772 4566 4586 3772
Size: 794 20 5442
Fragments arranged by size:
5442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DraIII CAC_nnn'GTG
Cuts at: 2972 2972
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2028 3182
Size: 54 1425 50 275 18 177 21 1154
Cuts at: 3182 3703 4592 6221 8
Size: 521 889 1629 43
Fragments arranged by size:
1629 1425 1154 889 521 275 177 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2136 3494 3503 4186 1353
Size: 768 15 1358 9 683 3423
Fragments arranged by size:
3423 1358 768 683 15 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 2220 2232 2601 4843 5217
Size: 1064 138 576 55 12 369 2242 374
Cuts at: 5217 5363 387
Size: 146 1280
Fragments arranged by size:
2242 1280 1064 576 374 369 146 138
55 12
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2498 2533 2715 3046 3384 4319
Size: 393 425 441 35 182 331 338 935
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 935 787 597 441 425 393 338
331 268 182 35
EcoO109I rG'GnC_Cy
Cuts at: 2473 2473
Size: 6256
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2778 2778
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2174 2274 2370 2436 5403 1678
Size: 496 100 96 66 2967 2531
Fragments arranged by size:
2967 2531 496 100 96 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2618 2650 3211
Size: 32 135 32 662 32 352 32 561
Cuts at: 3211 3243 4897 4929 4971 5003 1373
Size: 32 1654 32 42 32 2626
Fragments arranged by size:
2626 1654 662 561 352 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2432 2432
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 2616 5370 5554 1305
Size: 927 384 2754 184 2007
Fragments arranged by size:
2754 2007 927 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2865 4214 4573 4732 518
Size: 648 858 841 1349 359 159 2042
Fragments arranged by size:
2042 1349 858 841 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2193 2343 2391 2451 2497 2553 3672 4349
Size: 587 150 48 60 46 56 1119 677
Cuts at: 4349 5497 5742 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 587 245 150 60
56 48 46
NaeI GCC'GGC
Cuts at: 2612 2612
Size: 6256
NarI GG'CG_CC
Cuts at: 2433 2433
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 2958 3236 387
Size: 2571 278 3407
Fragments arranged by size:
3407 2571 278
NgoMIV G'CCGG_C
Cuts at: 2610 2610
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 3037 1052
Size: 1985 4271
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2864 3614 5159 1132
Size: 97 1635 750 1545 2229
Fragments arranged by size:
2229 1635 1545 750 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 2860 5155 2860
Size: 2295 3961
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 3608 830
Size: 2778 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 6256
SacII CC_GC'GG
Cuts at: 2498 2498
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 2686 3301 4504 6216 2686
Size: 615 1203 1712 2726
Fragments arranged by size:
2726 1712 1203 615
SfcI C'TryA_G
Cuts at: 826 1045 3604 4739 5420 5611 826
Size: 219 2559 1135 681 191 1471
Fragments arranged by size:
2559 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2434 2434
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2079 3583 1872
Size: 207 1504 4545
Fragments arranged by size:
4545 1504 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4360 5261 5523 820
Size: 197 926 2417 901 262 1553
Fragments arranged by size:
2417 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 2779 4180 5
Size: 47 2727 1401 2081
Fragments arranged by size:
2727 2081 1401 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 3695 513
Size: 552 939 483 1208 3074
Fragments arranged by size:
3074 1208 939 552 483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3376 4283 4300 4453 4638 5083 325
Size: 1255 1796 907 17 153 185 445 1498
Fragments arranged by size:
1796 1498 1255 907 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2684 3299 4502
Size: 274 80 33 51 1443 707 615 1203
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1203 707 615 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2837 2869 6216 6248 2837
Size: 32 3347 32 2845
Fragments arranged by size:
3347 2845 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2248 1316
Size: 932 5324
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2077 3581 1870
Size: 207 1504 4545
Fragments arranged by size:
4545 1504 207
XmnI GAAnn'nnTTC
Cuts at: 3369 4385 3369
Size: 1016 5240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SrfI SwaI XcmI