Restriction map for
pFN21AE2373
(Circular) (Six-base) MAPSORT of: pFN21AE2373.seq Check: 9077 from: 1 to: 8134
pFN21AE2373 8134 bp
With 182 enzymes: SgfI *
September 6, 2011 15:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3955 4538 5279 3955
Size: 583 741 6810
Fragments arranged by size:
6810 741 583
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5944 278
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5477 1937
Size: 3540 4594
Acc65I G'GTAC_C
Cuts at: 5455 5455
Size: 8134
AclI AA'CG_TT
Cuts at: 1563 6261 6634 1563
Size: 4698 373 3063
Fragments arranged by size:
4698 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 2599 4424 6197 7581 7849 1632
Size: 917 50 1825 1773 1384 268 1917
Fragments arranged by size:
1917 1825 1773 1384 917 268 50
AfeI AGC'GCT
Cuts at: 2280 3224 2280
Size: 944 7190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7937
AflIII A'CryG_T
Cuts at: 1203 2108 2738 5031 7033 8010 1203
Size: 905 630 2293 2002 977 1327
Fragments arranged by size:
2293 2002 1327 977 905 630
AgeI A'CCGG_T
Cuts at: 6965 6965
Size: 8134
AhdI GACnn_n'nnGTC
Cuts at: 6863 6863
Size: 8134
AleI CACnn'nnGTG
Cuts at: 1166 2585 3023 5405 1166
Size: 1419 438 2382 3895
Fragments arranged by size:
3895 2382 1419 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2393 4235 4374 1184
Size: 1209 1842 139 4944
Fragments arranged by size:
4944 1842 1209 139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 8102
AlwNI CAG_nnn'CTG
Cuts at: 2365 2527 2599 2896 4378 4573 4587 4900 5135
Size: 162 72 297 1482 195 14 313 235
Cuts at: 5135 5187 5306 5877 7449 2365
Size: 52 119 571 1572 3050
Fragments arranged by size:
3050 1572 1482 571 313 297 235 195
162 119 72 52 14
ApaI G_GGCC'C
Cuts at: 3494 3703 4105 4165 5298 3494
Size: 209 402 60 1133 6330
Fragments arranged by size:
6330 1133 402 209 60
ApaLI G'TGCA_C
Cuts at: 2629 6191 7347 2629
Size: 3562 1156 3416
Fragments arranged by size:
3562 3416 1156
ApoI r'AATT_y
Cuts at: 1493 3561 5443 5669 5821 7930 1493
Size: 2068 1882 226 152 2109 1697
Fragments arranged by size:
2109 2068 1882 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6688 160
Size: 872 5656 1606
Fragments arranged by size:
5656 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
AvaI C'yCGr_G
Cuts at: 1870 1943 4550 5459 1870
Size: 73 2607 909 4545
Fragments arranged by size:
4545 2607 909 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4911 4944 4911
Size: 33 8101
BamHI G'GATC_C
Cuts at: 3357 5464 5831 3357
Size: 2107 367 5660
Fragments arranged by size:
5660 2107 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2587 2995 3289 4002 4056
Size: 325 217 627 800 408 294 713 54
Cuts at: 4056 4511 5455 6910 618
Size: 455 944 1455 1842
Fragments arranged by size:
1842 1455 944 800 713 627 455 408
325 294 217 54
BanII G_rGCy'C
Cuts at: 1391 2059 2357 2413 3494 3648 3703 3794 4105
Size: 668 298 56 1081 154 55 91 311
Cuts at: 4105 4165 5298 5453 1391
Size: 60 1133 155 4072
Fragments arranged by size:
4072 1133 1081 668 311 298 155 154
91 60 56 55
BbeI G_GCGC'C
Cuts at: 4060 4515 4060
Size: 455 7679
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3145 3940 4528 5342 928
Size: 971 1246 795 588 814 3720
Fragments arranged by size:
3720 1246 971 814 795 588
BbvCI CC'TCA_GC
Cuts at: 3158 4640 5307 3158
Size: 1482 667 5985
Fragments arranged by size:
5985 1482 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6325 6359 1903
Size: 34 50 34 4304 34 3678
Fragments arranged by size:
4304 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4912 6026 7236 4912
Size: 1114 1210 5810
Fragments arranged by size:
5810 1210 1114
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8134
BfrBI ATG'CAT
Cuts at: 3312 3312
Size: 8134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2908 3004 3480
Size: 107 122 71 631 605 1236 96 476
Cuts at: 3480 3489 4014 5085 6745 136
Size: 9 525 1071 1660 1525
Fragments arranged by size:
1660 1525 1236 1071 631 605 525 476
122 107 96 71 9
BglII A'GATC_T
Cuts at: 8129 8129
Size: 8134
BlpI GC'TnA_GC
Cuts at: 4366 5551 4366
Size: 1185 6949
Bme1580I G_kGCm'C
Cuts at: 1790 2592 2633 3494 3703 4105 4165 4849 4864
Size: 802 41 861 209 402 60 684 15
Cuts at: 4864 5298 6195 7351 1790
Size: 434 897 1156 2573
Fragments arranged by size:
2573 1156 897 861 802 684 434 402
209 60 41 15
BmgBI CAC'GTC
Cuts at: 1318 3057 1318
Size: 1739 6395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2375 2494 2587
Size: 416 53 83 399 24 958 119 93
Cuts at: 2587 2812 5865 6823 442
Size: 225 3053 958 1753
Fragments arranged by size:
3053 1753 958 958 416 399 225 119
93 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3746 4796 1056
Size: 2690 1050 4394
Fragments arranged by size:
4394 2690 1050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 2873 2947 3122 3209 3318 3929 4234
Size: 976 658 74 175 87 109 611 305
Cuts at: 4234 4595 4679 4691 4802 4922 5267 5315 6794
Size: 361 84 12 111 120 345 48 1479
Cuts at: 6794 1239
Size: 2579
Fragments arranged by size:
2579 1479 976 658 611 361 345 305
175 120 111 109 87 84 74 48
12
Bpu10I CC'TnA_GC
Cuts at: 3158 4379 4640 4952 5307 3158
Size: 1221 261 312 355 5985
Fragments arranged by size:
5985 1221 355 312 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 2903 6259 7124 7422 2207
Size: 696 3356 865 298 2919
Fragments arranged by size:
3356 2919 865 696 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2037 3329 4655 6797 882
Size: 617 538 1292 1326 2142 2219
Fragments arranged by size:
2219 2142 1326 1292 617 538
BsaAI yAC'GTr
Cuts at: 493 1204 5431 5925 493
Size: 711 4227 494 2702
Fragments arranged by size:
4227 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3782 8128 3782
Size: 4346 3788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2455 2883 4057 4452
Size: 53 83 186 936 922 428 1174 395
Cuts at: 4452 4512 5941 6323 6958 275
Size: 60 1429 382 635 1451
Fragments arranged by size:
1451 1429 1174 936 922 635 428 395
382 186 83 60 53
BsaWI w'CCGG_w
Cuts at: 3303 3377 5418 6567 6965 7239 7386 7797 3303
Size: 74 2041 1149 398 274 147 411 3640
Fragments arranged by size:
3640 2041 1149 411 398 274 147 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4232 4262 4620 4650 1178
Size: 30 165 30 2829 30 358 30 4662
Fragments arranged by size:
4662 2829 358 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 2255 3137 3146 4037 4205 4247 4628
Size: 815 254 882 9 891 168 42 381
Cuts at: 4628 4919 4922 4925 4928 1186
Size: 291 3 3 3 4392
Fragments arranged by size:
4392 891 882 815 381 291 254 168
42 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2060 2275 2634 2807 3219 3225 3747 4097 4273
Size: 215 359 173 412 6 522 350 176
Cuts at: 4273 4414 4851 4890 5056 7337 7833 2060
Size: 141 437 39 166 2281 496 2361
Fragments arranged by size:
2361 2281 522 496 437 412 359 350
215 176 173 166 141 39 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3227 3983 4151 4505 4544 1163
Size: 573 1491 756 168 354 39 4753
Fragments arranged by size:
4753 1491 756 573 354 168 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3905 5584 6345 7373 1540
Size: 275 186 1904 1679 761 1028 2301
Fragments arranged by size:
2301 1904 1679 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 2633 2738 3822 6195 6280 7351 2538
Size: 95 105 1084 2373 85 1071 3321
Fragments arranged by size:
3321 2373 1084 1071 105 95 85
BsmI GAATG_Cn'
Cuts at: 5645 5738 5645
Size: 93 8041
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 4596 2099
Size: 2497 5637
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2357 2413 2538 2592 2633 2738
Size: 399 269 298 56 125 54 41 105
Cuts at: 2738 3494 3648 3703 3794 3822 4105 4165 4849
Size: 756 154 55 91 28 283 60 684
Cuts at: 4849 4864 5298 5453 6195 6280 7351 1391
Size: 15 434 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 756 742 684 434 399 298
283 269 155 154 125 105 91 85
60 56 55 54 41 28 15
BspEI T'CCGG_A
Cuts at: 3303 3377 7797 3303
Size: 74 4420 3640
Fragments arranged by size:
4420 3640 74
BspHI T'CATG_A
Cuts at: 6023 7753 6023
Size: 1730 6404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 3955 4105 4538 5216 5279 5489 844
Size: 1763 1348 150 433 678 63 210 3489
Fragments arranged by size:
3489 1763 1348 678 433 210 150 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2479 2854 3136 6021 1394
Size: 429 656 375 282 2885 3507
Fragments arranged by size:
3507 2885 656 429 375 282
BsrDI GCAATG_nn'
Cuts at: 65 1248 2983 3327 6629 6803 65
Size: 1183 1735 344 3302 174 1396
Fragments arranged by size:
3302 1735 1396 1183 344 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2555 2564 4728 4869 6778 6965 1700
Size: 110 745 9 2164 141 1909 187 2869
Fragments arranged by size:
2869 2164 1909 745 187 141 110 9
BsrGI T'GTAC_A
Cuts at: 96 5178 96
Size: 5082 3052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8134
BssSI C'ACGA_G
Cuts at: 4187 6194 7206 4187
Size: 2007 1012 5115
Fragments arranged by size:
5115 2007 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3427 4002 794
Size: 2633 575 4926
Fragments arranged by size:
4926 2633 575
BstBI TT'CG_AA
Cuts at: 6938 6938
Size: 8134
BstEII G'GTnAC_C
Cuts at: 1184 3140 3923 1184
Size: 1956 783 5395
Fragments arranged by size:
5395 1956 783
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 4942 5075 2674
Size: 2268 133 5733
Fragments arranged by size:
5733 2268 133
BstYI r'GATC_y
Cuts at: 1972 2721 3357 5464 5831 6216 6233 7685 7771
Size: 749 636 2107 367 385 17 1452 86
Cuts at: 7771 7783 8129 1972
Size: 12 346 1977
Fragments arranged by size:
2107 1977 1452 749 636 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3092 3129 3092
Size: 37 8097
BtgI C'CryG_G
Cuts at: 513 1065 2382 3080 4084 513
Size: 552 1317 698 1004 4563
Fragments arranged by size:
4563 1317 1004 698 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2479 3811 5239 487
Size: 683 56 1253 1332 1428 3382
Fragments arranged by size:
3382 1428 1332 1253 683 56
BtsI GCAGTG_nn'
Cuts at: 3239 5362 5650 6444 6464 3239
Size: 2123 288 794 20 4909
Fragments arranged by size:
4909 2123 794 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2740 2775 3310 3345 529
Size: 35 2176 35 535 35 5318
Fragments arranged by size:
5318 2176 535 35 35 35
DraI TTT'AAA
Cuts at: 5438 5438
Size: 8134
DraIII CAC_nnn'GTG
Cuts at: 2542 2542
Size: 8134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2921 3149 7141 809
Size: 507 1605 228 3992 1802
Fragments arranged by size:
3992 1802 1605 507 228
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2434 2460
Size: 54 1425 50 275 18 453 151 26
Cuts at: 2460 2553 2664 2748 2937 3261 3902 4044 4440
Size: 93 111 84 189 324 641 142 396
Cuts at: 4440 4767 4944 5581 6470 8099 8
Size: 327 177 637 889 1629 43
Fragments arranged by size:
1629 1425 889 641 637 453 396 327
324 275 189 177 151 142 111 93
84 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3902 5581 1537
Size: 275 2090 1679 4090
Fragments arranged by size:
4090 2090 1679 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2136 2963 3596 3944 6064 1353
Size: 711 72 827 633 348 2120 3423
Fragments arranged by size:
3423 2120 827 711 633 348 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2393 2659 3059 3185 3497 6721
Size: 1064 138 804 266 400 126 312 3224
Cuts at: 6721 7095 7241 387
Size: 374 146 1280
Fragments arranged by size:
3224 1280 1064 804 400 374 312 266
146 138 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2549 2599 2873 2947 3122 3209
Size: 393 583 334 50 274 74 175 87
Cuts at: 3209 3318 3929 4234 4424 4595 4679 4691 4802
Size: 109 611 305 190 171 84 12 111
Cuts at: 4802 4922 5267 5315 6197 6794 7581 7849 1239
Size: 120 345 48 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 611 597 583 393 345
334 305 274 268 190 175 171 120
111 109 87 84 74 50 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4955 4955
Size: 8134
EcoO109I rG'GnC_Cy
Cuts at: 2827 3491 3619 3699 3700 3718 3735 4101 4162
Size: 664 128 80 1 18 17 366 61
Cuts at: 4162 4375 4423 5042 5281 5295 5355 2827
Size: 213 48 619 239 14 60 5606
Fragments arranged by size:
5606 664 619 366 239 213 128 80
61 60 48 18 17 14 1
EcoRI G'AATT_C
Cuts at: 5443 5821 5443
Size: 378 7756
FspI TGC'GCA
Cuts at: 6640 6640
Size: 8134
HaeII r_GCGC'y
Cuts at: 1678 2282 2896 3183 3226 3252 3870 4060 4150
Size: 604 614 287 43 26 618 190 90
Cuts at: 4150 4159 4515 7281 1678
Size: 9 356 2766 2531
Fragments arranged by size:
2766 2531 618 614 604 356 287 190
90 43 26 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2677 2709 3388 3420 4232
Size: 32 135 32 1105 32 679 32 812
Cuts at: 4232 4264 6775 6807 6849 6881 1373
Size: 32 2511 32 42 32 2626
Fragments arranged by size:
2626 2511 1105 812 679 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5478 5724 669
Size: 988 281 3540 246 3079
Fragments arranged by size:
3540 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4325 748
Size: 3577 4557
HpaI GTT'AAC
Cuts at: 5724 5724
Size: 8134
KasI G'GCGC_C
Cuts at: 4056 4511 4056
Size: 455 7679
KpnI G_GTAC'C
Cuts at: 5459 5459
Size: 8134
MfeI C'AATT_G
Cuts at: 5733 5733
Size: 8134
MluI A'CGCG_T
Cuts at: 2108 8010 2108
Size: 5902 2232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4056 4840 7248 7432 1305
Size: 2751 784 2408 184 2007
Fragments arranged by size:
2751 2408 2007 784 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 2462 2666 4046 4946
Size: 54 1425 343 604 26 204 1380 900
Cuts at: 4946 10
Size: 3198
Fragments arranged by size:
3198 1425 1380 900 604 343 204 54
26
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2930 3023 3611 5036 5405 6092
Size: 648 1419 345 93 588 1425 369 687
Cuts at: 6092 6451 6610 518
Size: 359 159 2042
Fragments arranged by size:
2042 1425 1419 687 648 588 369 359
345 159 93
MspA1I CmG'CkG
Cuts at: 1606 2384 2800 3082 3162 3466 3751 3907 4174
Size: 778 416 282 80 304 285 156 267
Cuts at: 4174 4348 4358 4393 4438 4851 4876 4900 5550
Size: 174 10 35 45 413 25 24 650
Cuts at: 5550 6227 7375 7620 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 778 677 650 416 413 304
285 282 267 245 174 156 80 45
35 25 24 10
NarI GG'CG_CC
Cuts at: 4057 4512 4057
Size: 455 7679
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7582
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8134
NheI G'CTAG_C
Cuts at: 1052 3742 4792 1052
Size: 2690 1050 4394
Fragments arranged by size:
4394 2690 1050
NotI GC'GGCC_GC
Cuts at: 3902 5581 3902
Size: 1679 6455
NsiI A_TGCA'T
Cuts at: 3314 3314
Size: 8134
NspI r_CATG'y
Cuts at: 1132 1229 5035 5231 5492 7037 1132
Size: 97 3806 196 261 1545 2229
Fragments arranged by size:
3806 2229 1545 261 196 97
PasI CC'CwG_GG
Cuts at: 1348 4283 5300 1348
Size: 2935 1017 4182
Fragments arranged by size:
4182 2935 1017
PciI A'CATG_T
Cuts at: 5031 7033 5031
Size: 2002 6132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 5291 1108
Size: 1437 2746 3951
Fragments arranged by size:
3951 2746 1437
PfoI T'CCnGG_A
Cuts at: 2231 3555 3687 4308 2231
Size: 1324 132 621 6057
Fragments arranged by size:
6057 1324 621 132
PmeI GTTT'AAAC
Cuts at: 5438 5438
Size: 8134
PmlI CAC'GTG
Cuts at: 5431 5431
Size: 8134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2757 6203 6235 7716 7748 2725
Size: 32 3446 32 1481 32 3111
Fragments arranged by size:
3446 3111 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 3619 3735 4375 5042 2827
Size: 792 116 640 667 5919
Fragments arranged by size:
5919 792 667 640 116
PshAI GACnn'nnGTC
Cuts at: 1613 3341 5342 1613
Size: 1728 2001 4405
Fragments arranged by size:
4405 2001 1728
PsiI TTA'TAA
Cuts at: 5704 5704
Size: 8134
PspOMI G'GGCC_C
Cuts at: 3490 3699 4101 4161 5294 3490
Size: 209 402 60 1133 6330
Fragments arranged by size:
6330 1133 402 209 60
PstI C_TGCA'G
Cuts at: 830 2208 2472 2523 3432 3597 4230 4353 4791
Size: 1378 264 51 909 165 633 123 438
Cuts at: 4791 5352 5486 830
Size: 561 134 3478
Fragments arranged by size:
3478 1378 909 633 561 438 264 165
134 123 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8134
PvuII CAG'CTG
Cuts at: 3751 4174 4348 4393 4438 4876 4900 3751
Size: 423 174 45 45 438 24 6985
Fragments arranged by size:
6985 438 423 174 45 45 24
SacII CC_GC'GG
Cuts at: 2385 3083 2385
Size: 698 7436
SalI G'TCGA_C
Cuts at: 1936 5476 1936
Size: 3540 4594
SanDI GG'GwC_CC
Cuts at: 3619 3735 5042 3619
Size: 116 1307 6711
Fragments arranged by size:
6711 1307 116
SapI GCTCTTCn'nnn_
Cuts at: 2963 3596 3944 2963
Size: 633 348 7153
Fragments arranged by size:
7153 633 348
SbfI CC_TGCA'GG
Cuts at: 5486 5486
Size: 8134
ScaI AGT'ACT
Cuts at: 6382 8094 6382
Size: 1712 6422
SexAI A'CCwGG_T
Cuts at: 2538 4677 2538
Size: 2139 5995
SfcI C'TryA_G
Cuts at: 826 1045 2204 2468 2519 3428 3593 4129 4226
Size: 219 1159 264 51 909 165 536 97
Cuts at: 4226 4349 4787 5348 5482 6617 7298 7489 826
Size: 123 438 561 134 1135 681 191 1471
Fragments arranged by size:
1471 1159 1135 909 681 561 536 438
264 219 191 165 134 123 97 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 3480 3489 3480
Size: 9 8125
SfoI GGC'GCC
Cuts at: 4058 4513 4058
Size: 455 7679
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8134
SmaI CCC'GGG
Cuts at: 1872 5461 1872
Size: 3589 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2918 6238 7139 7401 820
Size: 197 926 279 696 3320 901 262 1553
Fragments arranged by size:
3320 1553 926 901 696 279 262 197
SnaBI TAC'GTA
Cuts at: 493 5925 493
Size: 5432 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8134
SphI G_CATG'C
Cuts at: 1132 5231 5492 1132
Size: 4099 261 3774
Fragments arranged by size:
4099 3774 261
SspI AAT'ATT
Cuts at: 5 52 6058 5
Size: 47 6006 2081
Fragments arranged by size:
6006 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 5372 1517
Size: 558 3297 4279
Fragments arranged by size:
4279 3297 558
StyI C'CwwG_G
Cuts at: 513 1065 2076 4935 5573 513
Size: 552 1011 2859 638 3074
Fragments arranged by size:
3074 2859 1011 638 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 3217 3233 3742 3949 5413 6161
Size: 1255 1068 569 16 509 207 1464 748
Cuts at: 6161 6178 6331 6516 6961 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1464 1255 1068 748 569 509 445
207 185 153 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5178 6380 8092
Size: 274 80 33 51 1443 3201 1202 1712
Cuts at: 8092 96
Size: 138
Fragments arranged by size:
3201 1712 1443 1202 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3828 3860 8094 8126 3828
Size: 32 4234 32 3836
Fragments arranged by size:
4234 3836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3055 1316
Size: 1739 6395
XbaI T'CTAG_A
Cuts at: 5470 5470
Size: 8134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3802 4297 4819 3802
Size: 495 522 7117
Fragments arranged by size:
7117 522 495
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8134
XmaI C'CCGG_G
Cuts at: 1870 5459 1870
Size: 3589 4545
XmnI GAAnn'nnTTC
Cuts at: 5384 6263 5384
Size: 879 7255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5942 276
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BplI BsiWI BstZ17I EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI
RsrII SacI SrfI SwaI