Restriction map for
pFN21AE2369
(Circular) (Six-base) MAPSORT of: pFN21AE2369.seq Check: 8396 from: 1 to: 6445
pFN21AE2369 6445 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 3009 3100 3766 3009
Size: 91 666 5688
Fragments arranged by size:
5688 666 91
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4508 5892 6160 1632
Size: 2876 1384 268 1917
Fragments arranged by size:
2876 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2797 820
Size: 197 1780 4468
Fragments arranged by size:
4468 1780 197
AflIII A'CryG_T
Cuts at: 1203 2900 3220 5344 6321 1203
Size: 1697 320 2124 977 1327
Fragments arranged by size:
2124 1697 1327 977 320
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 2494 5174 2494
Size: 2680 3765
AleI CACnn'nnGTG
Cuts at: 1166 3443 1166
Size: 2277 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AlwNI CAG_nnn'CTG
Cuts at: 2104 2162 2942 4188 5760 2104
Size: 58 780 1246 1572 2789
Fragments arranged by size:
2789 1572 1246 780 58
ApaLI G'TGCA_C
Cuts at: 3224 4502 5658 3224
Size: 1278 1156 4011
Fragments arranged by size:
4011 1278 1156
ApoI r'AATT_y
Cuts at: 1493 3368 3754 3980 4132 6241 1493
Size: 1875 386 226 152 2109 1697
Fragments arranged by size:
2109 1875 1697 386 226 152
AscI GG'CGCG_CC
Cuts at: 2380 2380
Size: 6445
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2198 2219 3770 1870
Size: 73 255 21 1551 4545
Fragments arranged by size:
4545 1551 255 73 21
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2480 3009 3100 3766
Size: 325 217 627 537 156 529 91 666
Cuts at: 3766 5221 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 666 627 537 529 325 217
156 91
BanII G_rGCy'C
Cuts at: 1391 3588 3764 1391
Size: 2197 176 4072
Fragments arranged by size:
4072 2197 176
BbeI G_GCGC'C
Cuts at: 2484 2484
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2921 3516 928
Size: 971 1022 595 3857
Fragments arranged by size:
3857 1022 971 595
BbvCI CC'TCA_GC
Cuts at: 2191 2191
Size: 6445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 4636 4670 1903
Size: 34 593 34 2072 34 3678
Fragments arranged by size:
3678 2072 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3235 4337 5547 3235
Size: 1102 1210 4133
Fragments arranged by size:
4133 1210 1102
BclI T'GATC_A
Cuts at: 1548 2559 3016 1548
Size: 1011 457 4977
Fragments arranged by size:
4977 1011 457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 2045 2408 3672 3862 2045
Size: 363 1264 190 4628
Fragments arranged by size:
4628 1264 363 190
Bme1580I G_kGCm'C
Cuts at: 1790 2310 3228 4506 5662 1790
Size: 520 918 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 918 520
BmgBI CAC'GTC
Cuts at: 1318 3658 1318
Size: 2340 4105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4176 5134 442
Size: 416 53 83 399 24 2759 958 1753
Fragments arranged by size:
2759 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2641 5105 1239
Size: 1059 343 2464 2579
Fragments arranged by size:
2579 2464 1059 343
Bpu10I CC'TnA_GC
Cuts at: 2191 2509 2191
Size: 318 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 4570 5435 5733 2074
Size: 2496 865 298 2786
Fragments arranged by size:
2786 2496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 5108 882
Size: 617 957 2652 2219
Fragments arranged by size:
2652 2219 957 617
BsaAI yAC'GTr
Cuts at: 493 1204 3223 4236 493
Size: 711 2019 1013 2702
Fragments arranged by size:
2702 2019 1013 711
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2481 2495 4252
Size: 53 83 186 936 498 450 14 1757
Cuts at: 4252 4634 5269 275
Size: 382 635 1451
Fragments arranged by size:
1757 1451 936 635 498 450 382 186
83 53 14
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2246 1178
Size: 30 165 30 813 30 5377
Fragments arranged by size:
5377 813 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2295 2848 1186
Size: 956 153 553 4783
Fragments arranged by size:
4783 956 553 153
BseYI C'CCAG_C
Cuts at: 2368 5648 6144 2368
Size: 3280 496 2669
Fragments arranged by size:
3280 2669 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5872
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2042 3895 4656 5684 1540
Size: 275 186 41 1853 761 1028 2301
Fragments arranged by size:
2301 1853 1028 761 275 186 41
BsiHKAI G_wGCw'C
Cuts at: 2226 2878 3228 4506 4591 5662 2226
Size: 652 350 1278 85 1071 3009
Fragments arranged by size:
3009 1278 1071 652 350 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2310 2878 3228 3588 3764 4506
Size: 399 436 84 568 350 360 176 742
Cuts at: 4506 4591 5662 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 436 399 360 350
176 85 84
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 3234 4334 6064 3234
Size: 1100 1730 3615
Fragments arranged by size:
3615 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2007 3800 844
Size: 1163 1793 3489
Fragments arranged by size:
3489 1793 1163
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2304 3676 4332 1394
Size: 429 221 260 1372 656 3507
Fragments arranged by size:
3507 1372 656 429 260 221
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2258 2376 5089 5276 1700
Size: 110 448 118 2713 187 2869
Fragments arranged by size:
2869 2713 448 187 118 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 2380 1925
Size: 455 5990
BssSI C'ACGA_G
Cuts at: 2078 4505 5517 2078
Size: 2427 1012 3006
Fragments arranged by size:
3006 2427 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 2162 794
Size: 1310 58 5077
Fragments arranged by size:
5077 1310 58
BstBI TT'CG_AA
Cuts at: 2391 5249 2391
Size: 2858 3587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 3775 4142 4527 4544 5996 6082 6094 6440
Size: 1803 367 385 17 1452 86 12 346
Cuts at: 6440 1972
Size: 1977
Fragments arranged by size:
1977 1803 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5893
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3065 487
Size: 683 56 1839 3867
Fragments arranged by size:
3867 1839 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 3961 4755 4775 2326
Size: 1635 794 20 3996
Fragments arranged by size:
3996 1635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2470 3656 5452 809
Size: 507 1154 1186 1796 1802
Fragments arranged by size:
1802 1796 1186 1154 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 3892 4781
Size: 54 1425 50 275 18 209 1853 889
Cuts at: 4781 6410 8
Size: 1629 43
Fragments arranged by size:
1853 1629 1425 889 275 209 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2039 3892 1537
Size: 275 227 1853 4090
Fragments arranged by size:
4090 1853 275 227
EarI CTCTTCn'nnn_
Cuts at: 1353 3515 3715 4375 1353
Size: 2162 200 660 3423
Fragments arranged by size:
3423 2162 660 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2280 5032 5406 5552 387
Size: 1064 138 691 2752 374 146 1280
Fragments arranged by size:
2752 1280 1064 691 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2641 4508 5105 5892 6160 1239
Size: 393 666 343 1867 597 787 268 1524
Fragments arranged by size:
1867 1524 787 666 597 393 343 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3691 3691
Size: 6445
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
EcoRV GAT'ATC
Cuts at: 3063 3063
Size: 6445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 6445
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 2249 2484 5592 1678
Size: 571 235 3108 2531
Fragments arranged by size:
3108 2531 571 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2480 2512 2843 2875 3229
Size: 32 135 32 908 32 331 32 354
Cuts at: 3229 3261 5086 5118 5160 5192 1373
Size: 32 1825 32 42 32 2626
Fragments arranged by size:
2626 1825 908 354 331 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 2480 2480
Size: 6445
KpnI G_GTAC'C
Cuts at: 3013 3104 3770 3013
Size: 91 666 5688
Fragments arranged by size:
5688 666 91
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 2259 5559 5743 1305
Size: 799 155 3300 184 2007
Fragments arranged by size:
3300 2007 799 184 155
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4623
Fragments arranged by size:
4623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2713 3443 4403 4762 4921 518
Size: 648 1547 730 960 359 159 2042
Fragments arranged by size:
2042 1547 960 730 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2162 2180 2762 3250 3275 3861 4538
Size: 407 149 18 582 488 25 586 677
Cuts at: 4538 5686 5931 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 586 582 488 407 245
149 25 18
NarI GG'CG_CC
Cuts at: 2481 2481
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5893
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 2039 3892 2039
Size: 1853 4592
NspI r_CATG'y
Cuts at: 1132 1229 2437 3803 5348 1132
Size: 97 1208 1366 1545 2229
Fragments arranged by size:
2229 1545 1366 1208 97
PasI CC'CwG_GG
Cuts at: 1348 3581 1348
Size: 2233 4212
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 1108
Size: 975 5470
PfoI T'CCnGG_A
Cuts at: 3692 3692
Size: 6445
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PmlI CAC'GTG
Cuts at: 3223 3223
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2345 2377 2345
Size: 32 6413
PstI C_TGCA'G
Cuts at: 830 2018 2109 2158 3797 830
Size: 1188 91 49 1639 3478
Fragments arranged by size:
3478 1639 1188 91 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2013 3250 3275 2013
Size: 1237 25 5183
Fragments arranged by size:
5183 1237 25
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2014 2105 2154 3180 3793 4928 5609
Size: 219 969 91 49 1026 613 1135 681
Cuts at: 5609 5800 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1026 969 681 613 219 191
91 49
SfoI GGC'GCC
Cuts at: 2482 2482
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 2376 1700
Size: 676 5769
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2797 4549 5450 5712 820
Size: 197 926 110 744 1752 901 262 1553
Fragments arranged by size:
1752 1553 926 901 744 262 197 110
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6445
StyI C'CwwG_G
Cuts at: 513 1065 3043 3884 513
Size: 552 1978 841 3074
Fragments arranged by size:
3074 1978 841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2480 2543 3140 4472 4489 4642 4827
Size: 1255 900 63 597 1332 17 153 185
Cuts at: 4827 5272 325
Size: 445 1498
Fragments arranged by size:
1498 1332 1255 900 597 445 185 153
63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2944 3283 4691
Size: 274 80 33 51 1443 967 339 1408
Cuts at: 4691 6403 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1408 967 339 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 6405 6437 2216
Size: 32 4157 32 2224
Fragments arranged by size:
4157 2224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 2861 3781 2861
Size: 920 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 3578 2764
Size: 814 5631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 4574 4574
Size: 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI
NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI