Restriction map for
pFN21AE2368
(Circular) (Six-base) MAPSORT of: pFN21AE2368.seq Check: 6275 from: 1 to: 10750
pFN21AE2368 10750 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6232 7755 6232
Size: 1523 9227
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5844 6597 8560 278
Size: 53 83 186 936 4308 753 1963 2468
Fragments arranged by size:
4308 2468 1963 936 753 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5838 8093 1937
Size: 3901 2255 4594
Fragments arranged by size:
4594 3901 2255
Acc65I G'GTAC_C
Cuts at: 6225 8071 6225
Size: 1846 8904
AclI AA'CG_TT
Cuts at: 1563 8877 9250 1563
Size: 7314 373 3063
Fragments arranged by size:
7314 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 3438 3642 4813 5529 6028 7309 7634
Size: 1100 706 204 1171 716 499 1281 325
Cuts at: 7634 8813 10197 10465 1632
Size: 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1281 1179 1171 1100 716 706
499 325 268 204
AfeI AGC'GCT
Cuts at: 2862 3302 4082 5664 2862
Size: 440 780 1582 7948
Fragments arranged by size:
7948 1582 780 440
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10553
AflIII A'CryG_T
Cuts at: 1203 2556 3005 6336 7071 7296 9649 10626 1203
Size: 1353 449 3331 735 225 2353 977 1327
Fragments arranged by size:
3331 2353 1353 1327 977 735 449 225
AgeI A'CCGG_T
Cuts at: 3021 9581 3021
Size: 6560 4190
AhdI GACnn_n'nnGTC
Cuts at: 8038 9479 8038
Size: 1441 9309
AleI CACnn'nnGTG
Cuts at: 1166 6131 1166
Size: 4965 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7763 1184
Size: 6579 4171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3733 3765 7619 7651 3733
Size: 32 3854 32 6832
Fragments arranged by size:
6832 3854 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3461 3931 4853 5283 5303 8493 10065 3461
Size: 470 922 430 20 3190 1572 4146
Fragments arranged by size:
4146 3190 1572 922 470 430 20
ApaI G_GGCC'C
Cuts at: 2683 2731 3924 3991 5894 2683
Size: 48 1193 67 1903 7539
Fragments arranged by size:
7539 1903 1193 67 48
ApaLI G'TGCA_C
Cuts at: 2419 5919 8807 9963 2419
Size: 3500 2888 1156 3206
Fragments arranged by size:
3500 3206 2888 1156
ApoI r'AATT_y
Cuts at: 1493 2627 4235 7400 8059 8285 8437 10546 1493
Size: 1134 1608 3165 659 226 152 2109 1697
Fragments arranged by size:
3165 2109 1697 1608 1134 659 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9304 160
Size: 872 8272 1606
Fragments arranged by size:
8272 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10750
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2144 2538 5120 7215 8075 1870
Size: 73 82 119 394 2582 2095 860 4545
Fragments arranged by size:
4545 2582 2095 860 394 119 82 73
BamHI G'GATC_C
Cuts at: 5125 6927 8080 8447 5125
Size: 1802 1153 367 7428
Fragments arranged by size:
7428 1802 1153 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2870 2960 3024 3691
Size: 325 217 627 243 840 90 64 667
Cuts at: 3691 5059 5114 5987 6225 7743 8071 9526 618
Size: 1368 55 873 238 1518 328 1455 1842
Fragments arranged by size:
1842 1518 1455 1368 873 840 667 627
328 325 243 238 217 90 64 55
BanII G_rGCy'C
Cuts at: 1391 2229 2241 2683 2731 3924 3991 5136 5451
Size: 838 12 442 48 1193 67 1145 315
Cuts at: 5451 5894 5941 6509 7235 7356 7674 8069 1391
Size: 443 47 568 726 121 318 395 4072
Fragments arranged by size:
4072 1193 1145 838 726 568 443 442
395 318 315 121 67 48 47 12
BbeI G_GCGC'C
Cuts at: 5991 5991
Size: 10750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 2770 3444 4261 4441 4750 5705
Size: 971 821 50 674 817 180 309 955
Cuts at: 5705 5740 928
Size: 35 5938
Fragments arranged by size:
5938 971 955 821 817 674 309 180
50 35
BbvCI CC'TCA_GC
Cuts at: 2786 3548 3992 4607 7151 2786
Size: 762 444 615 2544 6385
Fragments arranged by size:
6385 2544 762 615 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2529 2563 3059 3093 5309 5343 7802
Size: 34 592 34 496 34 2216 34 2459
Cuts at: 7802 7836 8941 8975 1903
Size: 34 1105 34 3678
Fragments arranged by size:
3678 2459 2216 1105 592 496 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3358 8642 9852 3358
Size: 5284 1210 4256
Fragments arranged by size:
5284 4256 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3919 4639 9361
Size: 107 122 71 631 605 2247 720 4722
Cuts at: 9361 136
Size: 1525
Fragments arranged by size:
4722 2247 1525 720 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2200 3165 7389 7659 7999 10745 2200
Size: 965 4224 270 340 2746 2205
Fragments arranged by size:
4224 2746 2205 965 340 270
BlpI GC'TnA_GC
Cuts at: 4820 5445 8167 4820
Size: 625 2722 7403
Fragments arranged by size:
7403 2722 625
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2423 2683 2731 3696 3721 3924 3991
Size: 243 390 260 48 965 25 203 67
Cuts at: 3991 4519 5117 5148 5210 5894 5923 6818 8811
Size: 528 598 31 62 684 29 895 1993
Cuts at: 8811 9967 1790
Size: 1156 2573
Fragments arranged by size:
2573 1993 1156 965 895 684 598 528
390 260 243 203 67 62 48 31
29 25
BmgBI CAC'GTC
Cuts at: 1318 7128 1318
Size: 5810 4940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3401 3726 3824
Size: 416 53 83 399 24 1984 325 98
Cuts at: 3824 4529 7179 8481 9439 442
Size: 705 2650 1302 958 1753
Fragments arranged by size:
2650 1984 1753 1302 958 705 416 399
325 98 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4544 1056
Size: 3488 7262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7856 7885 7856
Size: 29 10721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 4232 4865 5305 5474 5777 6277 7337
Size: 1025 1968 633 440 169 303 500 1060
Cuts at: 7337 7876 7949 9410 1239
Size: 539 73 1461 2579
Fragments arranged by size:
2579 1968 1461 1060 1025 633 539 500
440 303 169 73
Bpu10I CC'TnA_GC
Cuts at: 2786 3548 3992 4376 4607 7151 7827 2786
Size: 762 444 384 231 2544 676 5709
Fragments arranged by size:
5709 2544 762 676 444 384 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3311 5738 5753 8875 9740 10038 2936
Size: 375 2427 15 3122 865 298 3648
Fragments arranged by size:
3648 3122 2427 865 375 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3275 3603 3811 4700 4958 5384 9413
Size: 617 1776 328 208 889 258 426 4029
Cuts at: 9413 882
Size: 2219
Fragments arranged by size:
4029 2219 1776 889 617 426 328 258
208
BsaAI yAC'GTr
Cuts at: 493 1204 7852 8541 493
Size: 711 6648 689 2702
Fragments arranged by size:
6648 2702 711 689
BsaBI GATnn'nnATC
Cuts at: 4118 10744 4118
Size: 6626 4124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5841 5988 6594 7608
Size: 53 83 186 936 4308 147 606 1014
Cuts at: 7608 8557 8939 9574 275
Size: 949 382 635 1451
Fragments arranged by size:
4308 1451 1014 949 936 635 606 382
186 147 83 53
BsaWI w'CCGG_w
Cuts at: 2313 2364 3010 3021 5249 5269 6170 8023 9183
Size: 51 646 11 2228 20 901 1853 1160
Cuts at: 9183 9581 9855 10002 10413 2313
Size: 398 274 147 411 2650
Fragments arranged by size:
2650 2228 1853 1160 901 646 411 398
274 147 51 20 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3733 3763 5204 5234 5393
Size: 30 165 30 2330 30 1441 30 159
Cuts at: 5393 5423 6836 6866 7619 7649 1178
Size: 30 1413 30 753 30 4279
Fragments arranged by size:
4279 2330 1441 1413 753 165 159 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2165 3269 3302 3451 3479 3848 3923
Size: 976 3 1104 33 149 28 369 75
Cuts at: 3923 3980 4049 4256 5207 5228 5231 5299 5705
Size: 57 69 207 951 21 3 68 406
Cuts at: 5705 6005 6482 7139 7355 7643 7888 7897 1186
Size: 300 477 657 216 288 245 9 4039
Fragments arranged by size:
4039 1104 976 951 657 477 406 369
300 288 245 216 207 149 75 69
68 57 33 28 21 9 3 3
BseYI C'CCAG_C
Cuts at: 2166 2451 2999 3123 4077 4390 5009 5238 6502
Size: 285 548 124 954 313 619 229 1264
Cuts at: 6502 7137 7234 9953 10449 2166
Size: 635 97 2719 496 2467
Fragments arranged by size:
2719 2467 1264 954 635 619 548 496
313 285 229 124 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 4854 1163
Size: 573 801 2317 7059
Fragments arranged by size:
7059 2317 801 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7134 8200 8961 9989 1540
Size: 275 186 5133 1066 761 1028 2301
Fragments arranged by size:
5133 2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2423 3882 3979 4218 5451 5613 5923 5941
Size: 194 1459 97 239 1233 162 310 18
Cuts at: 5941 6825 7349 8811 8896 9967 2229
Size: 884 524 1462 85 1071 3012
Fragments arranged by size:
3012 1462 1459 1233 1071 884 524 310
239 194 162 97 85 18
BsmI GAATG_Cn'
Cuts at: 8261 8354 8261
Size: 93 10657
BsmBI CGTCTCn'nnnn_
Cuts at: 4076 5042 5848 4076
Size: 966 806 8978
Fragments arranged by size:
8978 966 806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2229 2241 2423 2683 2731 3696
Size: 399 243 196 12 182 260 48 965
Cuts at: 3696 3721 3882 3924 3979 3991 4218 4519 5117
Size: 25 161 42 55 12 227 301 598
Cuts at: 5117 5136 5148 5210 5451 5613 5894 5923 5941
Size: 19 12 62 241 162 281 29 18
Cuts at: 5941 6509 6818 6825 7235 7349 7356 7674 8069
Size: 568 309 7 410 114 7 318 395
Cuts at: 8069 8811 8896 9967 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 965 742 598 568 410 399
395 318 309 301 281 260 243 241
227 196 182 162 161 114 85 62
55 48 42 29 25 19 18 12
12 12 7 7
BspEI T'CCGG_A
Cuts at: 2364 3010 5269 8023 10413 2364
Size: 646 2259 2754 2390 2701
Fragments arranged by size:
2754 2701 2390 2259 646
BspHI T'CATG_A
Cuts at: 5640 6003 6459 6951 8639 10369 5640
Size: 363 456 492 1688 1730 6021
Fragments arranged by size:
6021 1730 1688 492 456 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2184 2955 4243 5600 6232 6702 7527
Size: 1193 147 771 1288 1357 632 470 825
Cuts at: 7527 7755 8105 844
Size: 228 350 3489
Fragments arranged by size:
3489 1357 1288 1193 825 771 632 470
350 228 147
BsrBI CCG'CTC
Cuts at: 1394 1823 2372 5695 8637 1394
Size: 429 549 3323 2942 3507
Fragments arranged by size:
3507 3323 2942 549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2650 3249 3525 3802 4308 5016 7213
Size: 1183 1402 599 276 277 506 708 2197
Cuts at: 7213 7302 7546 9245 9419 65
Size: 89 244 1699 174 1396
Fragments arranged by size:
2197 1699 1402 1396 1183 708 599 506
277 276 244 174 89
BsrFI r'CCGG_y
Cuts at: 1700 1810 3021 7133 9394 9581 1700
Size: 110 1211 4112 2261 187 2869
Fragments arranged by size:
4112 2869 2261 1211 187 110
BsrGI T'GTAC_A
Cuts at: 96 6339 7299 96
Size: 6243 960 3547
Fragments arranged by size:
6243 3547 960
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10750
BssSI C'ACGA_G
Cuts at: 3338 8810 9822 3338
Size: 5472 1012 4266
Fragments arranged by size:
5472 4266 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3632 4988 5592 7107 7548 7750 794
Size: 2838 1356 604 1515 441 202 3794
Fragments arranged by size:
3794 2838 1515 1356 604 441 202
BstBI TT'CG_AA
Cuts at: 9554 9554
Size: 10750
BstEII G'GTnAC_C
Cuts at: 1184 6628 1184
Size: 5444 5306
BstXI CCAn_nnnn'nTGG
Cuts at: 2586 4021 4062 5155 5872 6640 7315 2586
Size: 1435 41 1093 717 768 675 6021
Fragments arranged by size:
6021 1435 1093 768 717 675 41
BstYI r'GATC_y
Cuts at: 1972 2200 3165 5125 5460 6927 7389 7617 7659
Size: 228 965 1960 335 1467 462 228 42
Cuts at: 7659 7999 8080 8447 8832 8849 10301 10387 10399
Size: 340 81 367 385 17 1452 86 12
Cuts at: 10399 10745 1972
Size: 346 1977
Fragments arranged by size:
1977 1960 1467 1452 965 462 385 367
346 340 335 228 228 86 81 42
17 12
Bsu36I CC'TnA_GG
Cuts at: 2831 3149 2831
Size: 318 10432
BtgI C'CryG_G
Cuts at: 513 1065 2004 2104 2579 2883 3052 3234 3315
Size: 552 939 100 475 304 169 182 81
Cuts at: 3315 3541 3580 3766 6533 7239 7335 7524 7716
Size: 226 39 186 2767 706 96 189 192
Cuts at: 7716 513
Size: 3547
Fragments arranged by size:
3547 2767 939 706 552 475 304 226
192 189 186 182 169 100 96 81
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3259 5134 7828 487
Size: 683 56 2033 1875 2694 3409
Fragments arranged by size:
3409 2694 2033 1875 683 56
BtsI GCAGTG_nn'
Cuts at: 3683 5360 5662 5903 8266 9060 9080 3683
Size: 1677 302 241 2363 794 20 5353
Fragments arranged by size:
5353 2363 1677 794 302 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10715
DraI TTT'AAA
Cuts at: 6256 8054 6256
Size: 1798 8952
DraIII CAC_nnn'GTG
Cuts at: 4179 5605 4179
Size: 1426 9324
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2900 3151 3732 9757 809
Size: 507 1584 251 581 6025 1802
Fragments arranged by size:
6025 1802 1584 581 507 251
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3231 3754 4458
Size: 54 1425 50 275 18 1401 523 704
Cuts at: 4458 5157 5473 5862 6681 6705 7029 7131 7332
Size: 699 316 389 819 24 324 102 201
Cuts at: 7332 7713 8197 9086 10715 8
Size: 381 484 889 1629 43
Fragments arranged by size:
1629 1425 1401 889 819 704 699 523
484 389 381 324 316 275 201 102
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 7131 8197 1537
Size: 275 5319 1066 4090
Fragments arranged by size:
5319 4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2822 3104 4020 4205 4808 5178 6300
Size: 1052 417 282 916 185 603 370 1122
Cuts at: 6300 6869 6977 6998 7118 7256 7698 7787 7809
Size: 569 108 21 120 138 442 89 22
Cuts at: 7809 8680 1353
Size: 871 3423
Fragments arranged by size:
3423 1122 1052 916 871 603 569 442
417 370 282 185 138 120 108 89
22 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3092 3851 4424 6866 9337 9711
Size: 1064 138 1503 759 573 2442 2471 374
Cuts at: 9711 9857 387
Size: 146 1280
Fragments arranged by size:
2471 2442 1503 1280 1064 759 573 374
146 138
EcoICRI GAG'CTC
Cuts at: 2227 5449 5939 2227
Size: 3222 490 7038
Fragments arranged by size:
7038 3222 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2732 3438 3642 4232 4813 4865
Size: 393 632 468 706 204 590 581 52
Cuts at: 4865 5305 5474 5529 5777 6028 6277 7309 7337
Size: 440 169 55 248 251 249 1032 28
Cuts at: 7337 7634 7876 7949 8813 9410 10197 10465 1239
Size: 297 242 73 864 597 787 268 1524
Fragments arranged by size:
1524 1032 864 787 706 632 597 590
581 468 440 393 297 268 251 249
248 242 204 169 73 55 52 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3601 5474 6239 7361 7738 3601
Size: 1873 765 1122 377 6613
Fragments arranged by size:
6613 1873 1122 765 377
EcoO109I rG'GnC_Cy
Cuts at: 2248 2575 2680 2727 2728 3204 3343 3921 3988
Size: 327 105 47 1 476 139 578 67
Cuts at: 3988 4909 5263 5280 5374 5488 5557 5891 7582
Size: 921 354 17 94 114 69 334 1691
Cuts at: 7582 7837 2248
Size: 255 5161
Fragments arranged by size:
5161 1691 921 578 476 354 334 327
255 139 114 105 94 69 67 47
17 1
EcoRI G'AATT_C
Cuts at: 4235 8059 8437 4235
Size: 3824 378 6548
Fragments arranged by size:
6548 3824 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 5815 6604 6865 2326
Size: 3489 789 261 6211
Fragments arranged by size:
6211 3489 789 261
FspI TGC'GCA
Cuts at: 2849 5585 9256 2849
Size: 2736 3671 4343
Fragments arranged by size:
4343 3671 2736
HaeII r_GCGC'y
Cuts at: 1678 2864 3133 3304 4084 4211 5527 5666 5991
Size: 1186 269 171 780 127 1316 139 325
Cuts at: 5991 9897 1678
Size: 3906 2531
Fragments arranged by size:
3906 2531 1316 1186 780 325 269 171
139 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 3356 3388 3824
Size: 32 135 32 949 32 803 32 436
Cuts at: 3824 3856 4604 4636 4686 4718 5204 5236 5393
Size: 32 748 32 50 32 486 32 157
Cuts at: 5393 5425 7448 7480 8024 8056 9391 9423 9465
Size: 32 2023 32 544 32 1335 32 42
Cuts at: 9465 9497 1373
Size: 32 2626
Fragments arranged by size:
2626 2023 1335 949 803 748 544 486
436 157 135 50 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6202 7294 8094 8340 669
Size: 988 281 4264 1092 800 246 3079
Fragments arranged by size:
4264 3079 1092 988 800 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10750
HpaI GTT'AAC
Cuts at: 8340 8340
Size: 10750
KasI G'GCGC_C
Cuts at: 5987 5987
Size: 10750
KpnI G_GTAC'C
Cuts at: 6229 8075 6229
Size: 1846 8904
MfeI C'AATT_G
Cuts at: 8349 8349
Size: 10750
MluI A'CGCG_T
Cuts at: 2556 10626 2556
Size: 8070 2680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4237 4360 9864 10048 1305
Size: 2932 123 5504 184 2007
Fragments arranged by size:
5504 2932 2007 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3756 4460 5864 6683 6707
Size: 54 1425 343 1924 704 1404 819 24
Cuts at: 6707 7031 7334 10
Size: 324 303 3426
Fragments arranged by size:
3426 1924 1425 1404 819 704 343 324
303 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 4175 4550 4727 5153 5639 5963
Size: 648 2154 855 375 177 426 486 324
Cuts at: 5963 6056 6131 6458 6602 6638 7511 8708 9067
Size: 93 75 327 144 36 873 1197 359
Cuts at: 9067 9226 518
Size: 159 2042
Fragments arranged by size:
2154 2042 1197 873 855 648 486 426
375 359 327 324 177 159 144 93
75 36
MspA1I CmG'CkG
Cuts at: 1606 2106 2170 3582 3656 3985 4108 4885 4966
Size: 500 64 1412 74 329 123 777 81
Cuts at: 4966 5005 5013 5444 5616 6284 6771 7803 8166
Size: 39 8 431 172 668 487 1032 363
Cuts at: 8166 8843 9991 10236 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1412 1148 1032 777 677 668 500
487 431 363 329 245 172 123 81
74 64 39 8
NaeI GCC'GGC
Cuts at: 7135 7135
Size: 10750
NarI GG'CG_CC
Cuts at: 5988 5988
Size: 10750
NcoI C'CATG_G
Cuts at: 513 1065 2004 2579 3315 3766 7524 7716 513
Size: 552 939 575 736 451 3758 192 3547
Fragments arranged by size:
3758 3547 939 736 575 552 451 192
NdeI CA'TA_TG
Cuts at: 387 5743 387
Size: 5356 5394
NgoMIV G'CCGG_C
Cuts at: 7133 7133
Size: 10750
NheI G'CTAG_C
Cuts at: 1052 4540 1052
Size: 3488 7262
NotI GC'GGCC_GC
Cuts at: 8197 8197
Size: 10750
NspI r_CATG'y
Cuts at: 1132 1229 2410 3009 4579 6340 7075 7105 7300
Size: 97 1181 599 1570 1761 735 30 195
Cuts at: 7300 7772 8108 9653 1132
Size: 472 336 1545 2229
Fragments arranged by size:
2229 1761 1570 1545 1181 735 599 472
336 195 97 30
PasI CC'CwG_GG
Cuts at: 1348 3721 4996 5378 5561 5872 1348
Size: 2373 1275 382 183 311 6226
Fragments arranged by size:
6226 2373 1275 382 311 183
PciI A'CATG_T
Cuts at: 3005 6336 7071 7296 9649 3005
Size: 3331 735 225 2353 4106
Fragments arranged by size:
4106 3331 2353 735 225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4467 5283 5605 5811 6771 7552 1108
Size: 3359 816 322 206 960 781 4306
Fragments arranged by size:
4306 3359 960 816 781 322 206
PfoI T'CCnGG_A
Cuts at: 3306 3736 5276 5451 7314 3306
Size: 430 1540 175 1863 6742
Fragments arranged by size:
6742 1863 1540 430 175
PmeI GTTT'AAAC
Cuts at: 8054 8054
Size: 10750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 3733 3765 7619 7651 8819 8851 10332
Size: 32 753 32 3854 32 1168 32 1481
Cuts at: 10332 10364 2948
Size: 32 3334
Fragments arranged by size:
3854 3334 1481 1168 753 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2575 3204 5280 5488 7582 2248
Size: 327 629 2076 208 2094 5416
Fragments arranged by size:
5416 2094 2076 629 327 208
PshAI GACnn'nnGTC
Cuts at: 1613 6657 1613
Size: 5044 5706
PsiI TTA'TAA
Cuts at: 8320 8320
Size: 10750
PspOMI G'GGCC_C
Cuts at: 2679 2727 3920 3987 5890 2679
Size: 48 1193 67 1903 7539
Fragments arranged by size:
7539 1903 1193 67 48
PspXI vC'TCGA_Gb
Cuts at: 2538 2538
Size: 10750
PstI C_TGCA'G
Cuts at: 830 2181 2814 4254 6243 8102 830
Size: 1351 633 1440 1989 1859 3478
Fragments arranged by size:
3478 1989 1859 1440 1351 633
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10750
PvuII CAG'CTG
Cuts at: 2170 4885 5005 5013 5616 6284 6771 2170
Size: 2715 120 8 603 668 487 6149
Fragments arranged by size:
6149 2715 668 603 487 120 8
SacI G_AGCT'C
Cuts at: 2229 5451 5941 2229
Size: 3222 490 7038
Fragments arranged by size:
7038 3222 490
SacII CC_GC'GG
Cuts at: 2107 3583 2107
Size: 1476 9274
SalI G'TCGA_C
Cuts at: 1936 8092 1936
Size: 6156 4594
SanDI GG'GwC_CC
Cuts at: 2575 2575
Size: 10750
SapI GCTCTTCn'nnn_
Cuts at: 2405 2822 6300 6869 6998 2405
Size: 417 3478 569 129 6157
Fragments arranged by size:
6157 3478 569 417 129
SbfI CC_TGCA'GG
Cuts at: 2181 8102 2181
Size: 5921 4829
ScaI AGT'ACT
Cuts at: 5798 6482 6845 8998 10710 5798
Size: 684 363 2153 1712 5838
Fragments arranged by size:
5838 2153 1712 684 363
SexAI A'CCwGG_T
Cuts at: 3213 4263 3213
Size: 1050 9700
SfcI C'TryA_G
Cuts at: 826 1045 2177 2263 2810 4250 6239 7984 8098
Size: 219 1132 86 547 1440 1989 1745 114
Cuts at: 8098 9233 9914 10105 826
Size: 1135 681 191 1471
Fragments arranged by size:
1989 1745 1471 1440 1135 1132 681 547
219 191 114 86
SfoI GGC'GCC
Cuts at: 5989 5989
Size: 10750
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10750
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10750
SmaI CCC'GGG
Cuts at: 1872 5122 8077 1872
Size: 3250 2955 4545
Fragments arranged by size:
4545 3250 2955
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2538 2951 3326 5717 5768 8854
Size: 197 926 595 413 375 2391 51 3086
Cuts at: 8854 9755 10017 820
Size: 901 262 1553
Fragments arranged by size:
3086 2391 1553 926 901 595 413 375
262 197 51
SnaBI TAC'GTA
Cuts at: 493 8541 493
Size: 8048 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10750
SphI G_CATG'C
Cuts at: 1132 4579 7105 7772 8108 1132
Size: 3447 2526 667 336 3774
Fragments arranged by size:
3774 3447 2526 667 336
SspI AAT'ATT
Cuts at: 5 52 8674 5
Size: 47 8622 2081
Fragments arranged by size:
8622 2081 47
StuI AGG'CCT
Cuts at: 1517 2183 4626 6587 1517
Size: 666 2443 1961 5680
Fragments arranged by size:
5680 2443 1961 666
StyI C'CwwG_G
Cuts at: 513 1065 2004 2579 3315 3384 3766 6189 6921
Size: 552 939 575 736 69 382 2423 732
Cuts at: 6921 7524 7716 7879 8189 513
Size: 603 192 163 310 3074
Fragments arranged by size:
3074 2423 939 736 732 603 575 552
382 310 192 163 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 3299 5764 7426 8777 8794 8947
Size: 1255 705 1014 2465 1662 1351 17 153
Cuts at: 8947 9132 9577 325
Size: 185 445 1498
Fragments arranged by size:
2465 1662 1498 1351 1255 1014 705 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 5796 6180
Size: 274 80 33 51 1443 510 3309 384
Cuts at: 6180 6339 6480 6843 7299 8996 10708 96
Size: 159 141 363 456 1697 1712 138
Fragments arranged by size:
3309 1712 1697 1443 510 456 384 363
274 159 141 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5902 5934 10710 10742 5902
Size: 32 4776 32 5910
Fragments arranged by size:
5910 4776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7937 1316
Size: 6621 4129
XbaI T'CTAG_A
Cuts at: 8086 8086
Size: 10750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 3358 3764 5568 6499 7024 7582 2881
Size: 477 406 1804 931 525 558 6049
Fragments arranged by size:
6049 1804 931 558 525 477 406
XhoI C'TCGA_G
Cuts at: 1943 2538 1943
Size: 595 10155
XmaI C'CCGG_G
Cuts at: 1870 5120 8075 1870
Size: 3250 2955 4545
Fragments arranged by size:
4545 3250 2955
XmnI GAAnn'nnTTC
Cuts at: 4352 4802 5798 6779 6845 8879 4352
Size: 450 996 981 66 2034 6223
Fragments arranged by size:
6223 2034 996 981 450 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5842 6595 8558 276
Size: 53 83 186 936 4308 753 1963 2468
Fragments arranged by size:
4308 2468 1963 936 753 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SfiI
SrfI SwaI