Restriction map for
pFN21AE2367
(Circular) (Six-base) MAPSORT of: pFN21AE2367.seq Check: 6139 from: 1 to: 11140
pFN21AE2367 11140 bp
With 182 enzymes: SgfI *
August 4, 2011 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3196 3196
Size: 11140
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2076 5310 8950 278
Size: 53 83 186 936 540 3234 3640 2468
Fragments arranged by size:
3640 3234 2468 936 540 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 8483 1937
Size: 139 6407 4594
Fragments arranged by size:
6407 4594 139
Acc65I G'GTAC_C
Cuts at: 8461 8461
Size: 11140
AclI AA'CG_TT
Cuts at: 1563 6740 9267 9640 1563
Size: 5177 2527 373 3063
Fragments arranged by size:
5177 3063 2527 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2267 2538 4256 4837 5183 5933 6632
Size: 434 201 271 1718 581 346 750 699
Cuts at: 6632 6645 6801 7469 9203 10587 10855 1632
Size: 13 156 668 1734 1384 268 1917
Fragments arranged by size:
1917 1734 1718 1384 750 699 668 581
434 346 271 268 201 156 13
AflII C'TTAA_G
Cuts at: 820 1017 6428 820
Size: 197 5411 5532
Fragments arranged by size:
5532 5411 197
AflIII A'CryG_T
Cuts at: 1203 2721 3387 3855 5190 6896 10039 11016 1203
Size: 1518 666 468 1335 1706 3143 977 1327
Fragments arranged by size:
3143 1706 1518 1335 1327 977 666 468
AgeI A'CCGG_T
Cuts at: 9971 9971
Size: 11140
AhdI GACnn_n'nnGTC
Cuts at: 3372 4643 5754 8274 9869 3372
Size: 1271 1111 2520 1595 4643
Fragments arranged by size:
4643 2520 1595 1271 1111
AleI CACnn'nnGTG
Cuts at: 1166 5287 1166
Size: 4121 7019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4346 4378 4346
Size: 32 11108
AlwNI CAG_nnn'CTG
Cuts at: 3893 6668 7186 8023 8041 8883 10455 3893
Size: 2775 518 837 18 842 1572 4578
Fragments arranged by size:
4578 2775 1572 842 837 518 18
ApaI G_GGCC'C
Cuts at: 2998 6637 2998
Size: 3639 7501
ApaLI G'TGCA_C
Cuts at: 3066 9197 10353 3066
Size: 6131 1156 3853
Fragments arranged by size:
6131 3853 1156
ApoI r'AATT_y
Cuts at: 1493 2554 3788 4263 4838 4909 6574 6805 8449
Size: 1061 1234 475 575 71 1665 231 1644
Cuts at: 8449 8675 8827 10936 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1665 1644 1234 1061 575 475
231 226 152 71
AseI AT'TA_AT
Cuts at: 160 1032 4082 9694 160
Size: 872 3050 5612 1606
Fragments arranged by size:
5612 3050 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11140
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2983 3873 8465 1870
Size: 73 117 923 890 4592 4545
Fragments arranged by size:
4592 4545 923 890 117 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2971 3004 3844 3877 2971
Size: 33 840 33 10234
Fragments arranged by size:
10234 840 33 33
BamHI G'GATC_C
Cuts at: 2766 3692 4927 8143 8470 8837 2766
Size: 926 1235 3216 327 367 5069
Fragments arranged by size:
5069 3216 1235 926 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7592 8461 9916 618
Size: 325 217 627 5805 869 1455 1842
Fragments arranged by size:
5805 1842 1455 869 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2998 3032 5419 6637 7221 8069 8459 1391
Size: 1607 34 2387 1218 584 848 390 4072
Fragments arranged by size:
4072 2387 1607 1218 848 584 390 34
BbeI G_GCGC'C
Cuts at: 7596 7596
Size: 11140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3829 928
Size: 971 1930 8239
Fragments arranged by size:
8239 1930 971
BbvCI CC'TCA_GC
Cuts at: 4963 7773 4963
Size: 2810 8330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7611 7645 9331 9365 1903
Size: 34 5674 34 1686 34 3678
Fragments arranged by size:
5674 3678 1686 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7797 9032 10242 7797
Size: 1235 1210 8695
Fragments arranged by size:
8695 1235 1210
BclI T'GATC_A
Cuts at: 1548 2481 4393 4699 6003 7104 1548
Size: 933 1912 306 1304 1101 5584
Fragments arranged by size:
5584 1912 1304 1101 933 306
BfrBI ATG'CAT
Cuts at: 4785 6764 4785
Size: 1979 9161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3015 7922 9751
Size: 107 122 71 631 605 1343 4907 1829
Cuts at: 9751 136
Size: 1525
Fragments arranged by size:
4907 1829 1525 1343 631 605 122 107
71
BglII A'GATC_T
Cuts at: 11135 11135
Size: 11140
BlpI GC'TnA_GC
Cuts at: 2176 2921 7260 8557 2176
Size: 745 4339 1297 4759
Fragments arranged by size:
4759 4339 1297 745
Bme1580I G_kGCm'C
Cuts at: 1790 2902 2998 3070 6637 8133 9201 10357 1790
Size: 1112 96 72 3567 1496 1068 1156 2573
Fragments arranged by size:
3567 2573 1496 1156 1112 1068 96 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6610 6640 8871
Size: 416 53 83 399 24 5193 30 2231
Cuts at: 8871 9829 442
Size: 958 1753
Fragments arranged by size:
5193 2231 1753 958 416 399 83 53
30 24
BmtI G_CTAG'C
Cuts at: 1056 2714 1056
Size: 1658 9482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4978 4978
Size: 11140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2617 3620 4011 4358 4373 4594 5321
Size: 942 436 1003 391 347 15 221 727
Cuts at: 5321 5978 6335 6455 6523 6977 7163 7212 7488
Size: 657 357 120 68 454 186 49 276
Cuts at: 7488 7498 7894 8177 8357 9800 1239
Size: 10 396 283 180 1443 2579
Fragments arranged by size:
2579 1443 1003 942 727 657 454 436
396 391 357 347 283 276 221 186
180 120 68 49 15 10
Bpu10I CC'TnA_GC
Cuts at: 4963 7588 7773 4963
Size: 2625 185 8330
Fragments arranged by size:
8330 2625 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5424 5978 7324 9265 10130 10428 5424
Size: 554 1346 1941 865 298 6136
Fragments arranged by size:
6136 1941 1346 865 554 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2032 4839 6995 7003 8146 8282 9803
Size: 617 533 2807 2156 8 1143 136 1521
Cuts at: 9803 882
Size: 2219
Fragments arranged by size:
2807 2219 2156 1521 1143 617 533 136
8
BsaAI yAC'GTr
Cuts at: 493 1204 5191 8931 493
Size: 711 3987 3740 2702
Fragments arranged by size:
3987 3740 2702 711
BsaBI GATnn'nnATC
Cuts at: 2805 4398 7353 11134 2805
Size: 1593 2955 3781 2811
Fragments arranged by size:
3781 2955 2811 1593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2905 5307 5364
Size: 53 83 186 936 540 832 2402 57
Cuts at: 5364 7593 8947 9329 9964 275
Size: 2229 1354 382 635 1451
Fragments arranged by size:
2402 2229 1451 1354 936 832 635 540
382 186 83 57 53
BsaWI w'CCGG_w
Cuts at: 9573 9971 10245 10392 10803 9573
Size: 398 274 147 411 9910
Fragments arranged by size:
9910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 4346 4362 4376
Size: 30 165 30 1212 30 1701 16 14
Cuts at: 4376 4392 6975 7005 8108 8138 1178
Size: 16 2583 30 1103 30 4180
Fragments arranged by size:
4180 2583 1701 1212 1103 165 30 30
30 30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3891 5098 6622 7130 7217 7393 8180 1186
Size: 2705 1207 1524 508 87 176 787 4146
Fragments arranged by size:
4146 2705 1524 1207 787 508 176 87
BseYI C'CCAG_C
Cuts at: 2999 4990 5152 6302 6718 7051 7464 7898 8208
Size: 1991 162 1150 416 333 413 434 310
Cuts at: 8208 8217 8223 8235 8241 8295 8334 10343 10839
Size: 9 6 12 6 54 39 2009 496
Cuts at: 10839 2999
Size: 3300
Fragments arranged by size:
3300 2009 1991 1150 496 434 416 413
333 310 162 54 39 12 9 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3056 3333 5113 5333 6623 8091 1163
Size: 573 1320 277 1780 220 1290 1468 4212
Fragments arranged by size:
4212 1780 1468 1320 1290 573 277 220
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3908 7926 8355 8590 9351 10379
Size: 275 186 1907 4018 429 235 761 1028
Cuts at: 10379 1540
Size: 2301
Fragments arranged by size:
4018 2301 1907 1028 761 429 275 235
186
BsiHKAI G_wGCw'C
Cuts at: 3070 4283 5419 6614 9201 9286 10357 3070
Size: 1213 1136 1195 2587 85 1071 3853
Fragments arranged by size:
3853 2587 1213 1195 1136 1071 85
BsmI GAATG_Cn'
Cuts at: 4012 5650 8651 8744 4012
Size: 1638 3001 93 6408
Fragments arranged by size:
6408 3001 1638 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2902 2998 3032 3070 4283 5419 6614
Size: 399 1112 96 34 38 1213 1136 1195
Cuts at: 6614 6637 7221 8069 8133 8459 9201 9286 10357
Size: 23 584 848 64 326 742 85 1071
Cuts at: 10357 1391
Size: 2174
Fragments arranged by size:
2174 1213 1195 1136 1112 1071 848 742
584 399 326 96 85 64 38 34
23
BspEI T'CCGG_A
Cuts at: 10803 10803
Size: 11140
BspHI T'CATG_A
Cuts at: 4165 4938 5049 9029 10759 4165
Size: 773 111 3980 1730 4546
Fragments arranged by size:
4546 3980 1730 773 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3196 3250 6431 8022 8495 844
Size: 2352 54 3181 1591 473 3489
Fragments arranged by size:
3489 3181 2352 1591 473 54
BsrBI CCG'CTC
Cuts at: 1394 1823 9027 1394
Size: 429 7204 3507
Fragments arranged by size:
7204 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2696 5265 5779 6557 8292 9635 9809
Size: 1183 1448 2569 514 778 1735 1343 174
Cuts at: 9809 65
Size: 1396
Fragments arranged by size:
2569 1735 1448 1396 1343 1183 778 514
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9784 9971 1700
Size: 110 7974 187 2869
Fragments arranged by size:
7974 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5807 96
Size: 5711 5429
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11140
BssSI C'ACGA_G
Cuts at: 6184 7451 9200 10212 6184
Size: 1267 1749 1012 7112
Fragments arranged by size:
7112 1749 1267 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6547 8323 794
Size: 5753 1776 3611
Fragments arranged by size:
5753 3611 1776
BstBI TT'CG_AA
Cuts at: 4913 9944 4913
Size: 5031 6109
BstEII G'GTnAC_C
Cuts at: 1184 3912 1184
Size: 2728 8412
BstXI CCAn_nnnn'nTGG
Cuts at: 3586 3586
Size: 11140
BstYI r'GATC_y
Cuts at: 1972 2766 3196 3692 4927 4998 6776 8143 8470
Size: 794 430 496 1235 71 1778 1367 327
Cuts at: 8470 8837 9222 9239 10691 10777 10789 11135 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1778 1452 1367 1235 794 496 430
385 367 346 327 86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 7200 8139 7200
Size: 939 10201
BtgI C'CryG_G
Cuts at: 513 1065 2004 3441 3579 3908 7022 513
Size: 552 939 1437 138 329 3114 4631
Fragments arranged by size:
4631 3114 1437 939 552 329 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2658 4936 5647 6241 7614 7848
Size: 683 56 1432 2278 711 594 1373 234
Cuts at: 7848 487
Size: 3779
Fragments arranged by size:
3779 2278 1432 1373 711 683 594 234
56
BtsI GCAGTG_nn'
Cuts at: 3321 3891 5969 7184 7888 8656 9450 9470 3321
Size: 570 2078 1215 704 768 794 20 4991
Fragments arranged by size:
4991 2078 1215 794 768 704 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11105
DraI TTT'AAA
Cuts at: 5601 8444 5601
Size: 2843 8297
DraIII CAC_nnn'GTG
Cuts at: 6618 7437 6618
Size: 819 10321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10147 809
Size: 507 8831 1802
Fragments arranged by size:
8831 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2601 3905
Size: 54 1425 50 275 18 177 594 1304
Cuts at: 3905 5016 6042 7923 8352 8587 9476 11105 8
Size: 1111 1026 1881 429 235 889 1629 43
Fragments arranged by size:
1881 1629 1425 1304 1111 1026 889 594
429 275 235 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3905 7923 8352 8587 1537
Size: 275 2093 4018 429 235 4090
Fragments arranged by size:
4090 4018 2093 429 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 3599 3815 4055 4271 4856 5340 5414 6149
Size: 2246 216 240 216 585 484 74 735
Cuts at: 6149 6280 6303 6777 7093 7203 7209 7620 9070
Size: 131 23 474 316 110 6 411 1450
Cuts at: 9070 1353
Size: 3423
Fragments arranged by size:
3423 2246 1450 735 585 484 474 411
316 240 216 216 131 110 74 23
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3815 6557 9727 10101 10247 387
Size: 1064 138 2226 2742 3170 374 146 1280
Fragments arranged by size:
3170 2742 2226 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5417 5417
Size: 11140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2181 2267 2538 2617 3620 4011
Size: 393 434 115 86 271 79 1003 391
Cuts at: 4011 4256 4358 4373 4594 4837 5183 5321 5933
Size: 245 102 15 221 243 346 138 612
Cuts at: 5933 5978 6335 6455 6523 6632 6645 6801 6977
Size: 45 357 120 68 109 13 156 176
Cuts at: 6977 7163 7212 7469 7488 7498 7894 8177 8357
Size: 186 49 257 19 10 396 283 180
Cuts at: 8357 9203 9800 10587 10855 1239
Size: 846 597 787 268 1524
Fragments arranged by size:
1524 1003 846 787 612 597 434 396
393 391 357 346 283 271 268 257
245 243 221 186 180 176 156 138
120 115 109 102 86 79 68 49
45 19 15 13 10
EcoNI CCTnn'n_nnAGG
Cuts at: 8138 8138
Size: 11140
EcoO109I rG'GnC_Cy
Cuts at: 2086 2994 2995 3745 6022 6634 2086
Size: 908 1 750 2277 612 6592
Fragments arranged by size:
6592 2277 908 750 612 1
EcoRI G'AATT_C
Cuts at: 8449 8827 8449
Size: 378 10762
EcoRV GAT'ATC
Cuts at: 2574 2585 4255 5656 2574
Size: 11 1670 1401 8058
Fragments arranged by size:
8058 1670 1401 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 5493 2152
Size: 3341 7799
FspI TGC'GCA
Cuts at: 7755 9646 7755
Size: 1891 9249
HaeII r_GCGC'y
Cuts at: 1678 2053 4672 7588 7596 10287 1678
Size: 375 2619 2916 8 2691 2531
Fragments arranged by size:
2916 2691 2619 2531 375 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3103 3135 3358 3390 3439
Size: 32 135 32 1531 32 223 32 49
Cuts at: 3439 3471 3650 3682 3824 3856 3964 3996 4629
Size: 32 179 32 142 32 108 32 633
Cuts at: 4629 4661 5740 5772 6014 6046 6830 6862 7208
Size: 32 1079 32 242 32 784 32 346
Cuts at: 7208 7240 7405 7437 8017 8049 8260 8292 9781
Size: 32 165 32 580 32 211 32 1489
Cuts at: 9781 9813 9855 9887 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 1489 1079 784 633 580 346
242 223 211 179 165 142 135 108
49 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5759 6395 6484 6673 8484 8730
Size: 988 281 3821 636 89 189 1811 246
Cuts at: 8730 669
Size: 3079
Fragments arranged by size:
3821 3079 1811 988 636 281 246 189
89
HindIII A'AGCT_T
Cuts at: 748 2249 4447 7655 748
Size: 1501 2198 3208 4233
Fragments arranged by size:
4233 3208 2198 1501
HpaI GTT'AAC
Cuts at: 6484 8730 6484
Size: 2246 8894
KasI G'GCGC_C
Cuts at: 7592 7592
Size: 11140
KpnI G_GTAC'C
Cuts at: 8465 8465
Size: 11140
MfeI C'AATT_G
Cuts at: 8739 8739
Size: 11140
MluI A'CGCG_T
Cuts at: 11016 11016
Size: 11140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3372 3738 4370 7738 7959 10254 10438 1305
Size: 2067 366 632 3368 221 2295 184 2007
Fragments arranged by size:
3368 2295 2067 2007 632 366 221 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 10
Size: 54 1425 343 771 8547
Fragments arranged by size:
8547 1425 771 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3584 3856 5073 5287 5519 9098 9457
Size: 648 2418 272 1217 214 232 3579 359
Cuts at: 9457 9616 518
Size: 159 2042
Fragments arranged by size:
3579 2418 2042 1217 648 359 272 232
214 159
MspA1I CmG'CkG
Cuts at: 1606 2044 3040 3910 4234 5501 6302 7259 7582
Size: 438 996 870 324 1267 801 957 323
Cuts at: 7582 8556 9233 10381 10626 1606
Size: 974 677 1148 245 2120
Fragments arranged by size:
2120 1267 1148 996 974 957 870 801
677 438 324 323 245
NarI GG'CG_CC
Cuts at: 7593 7593
Size: 11140
NcoI C'CATG_G
Cuts at: 513 1065 2004 3441 3579 513
Size: 552 939 1437 138 8074
Fragments arranged by size:
8074 1437 939 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11140
NheI G'CTAG_C
Cuts at: 1052 2710 1052
Size: 1658 9482
NotI GC'GGCC_GC
Cuts at: 8587 8587
Size: 11140
NsiI A_TGCA'T
Cuts at: 4787 6766 4787
Size: 1979 9161
NspI r_CATG'y
Cuts at: 1132 1229 2725 3859 4748 5321 5391 6679 6718
Size: 97 1496 1134 889 573 70 1288 39
Cuts at: 6718 6900 7576 8498 10043 1132
Size: 182 676 922 1545 2229
Fragments arranged by size:
2229 1545 1496 1288 1134 922 889 676
573 182 97 70 39
PasI CC'CwG_GG
Cuts at: 1348 6770 1348
Size: 5422 5718
PciI A'CATG_T
Cuts at: 2721 3855 6896 10039 2721
Size: 1134 3041 3143 3822
Fragments arranged by size:
3822 3143 3041 1134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 7437 1108
Size: 6329 4811
PfoI T'CCnGG_A
Cuts at: 2262 2727 2769 3597 2262
Size: 465 42 828 9805
Fragments arranged by size:
9805 828 465 42
PmeI GTTT'AAAC
Cuts at: 8444 8444
Size: 11140
PmlI CAC'GTG
Cuts at: 5191 5191
Size: 11140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 9209 9241 10722 10754 4346
Size: 32 4831 32 1481 32 4732
Fragments arranged by size:
4831 4732 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 6022 2086
Size: 3936 7204
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11140
PsiI TTA'TAA
Cuts at: 8710 8710
Size: 11140
PspOMI G'GGCC_C
Cuts at: 2994 6633 2994
Size: 3639 7501
PspXI vC'TCGA_Gb
Cuts at: 2983 2983
Size: 11140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4849 4881 4849
Size: 32 11108
PstI C_TGCA'G
Cuts at: 830 2841 3207 5871 6109 7531 7665 7800 8019
Size: 2011 366 2664 238 1422 134 135 219
Cuts at: 8019 8492 830
Size: 473 3478
Fragments arranged by size:
3478 2664 2011 1422 473 366 238 219
135 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11140
PvuII CAG'CTG
Cuts at: 3040 4234 3040
Size: 1194 9946
SacI G_AGCT'C
Cuts at: 5419 5419
Size: 11140
SacII CC_GC'GG
Cuts at: 3911 3911
Size: 11140
SalI G'TCGA_C
Cuts at: 1936 8482 1936
Size: 6546 4594
SapI GCTCTTCn'nnn_
Cuts at: 3815 4271 6303 7209 3815
Size: 456 2032 906 7746
Fragments arranged by size:
7746 2032 906 456
SbfI CC_TGCA'GG
Cuts at: 7531 8492 7531
Size: 961 10179
ScaI AGT'ACT
Cuts at: 9388 11100 9388
Size: 1712 9428
SexAI A'CCwGG_T
Cuts at: 3798 3798
Size: 11140
SfcI C'TryA_G
Cuts at: 826 1045 2837 3203 5026 5220 5867 6105 6624
Size: 219 1792 366 1823 194 647 238 519
Cuts at: 6624 6972 7527 7577 7661 7796 8015 8057 8488
Size: 348 555 50 84 135 219 42 431
Cuts at: 8488 9623 10304 10495 826
Size: 1135 681 191 1471
Fragments arranged by size:
1823 1792 1471 1135 681 647 555 519
431 366 348 238 219 219 194 191
135 84 50 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3015 3015
Size: 11140
SfoI GGC'GCC
Cuts at: 7594 7594
Size: 11140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11140
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11140
SmaI CCC'GGG
Cuts at: 1872 8467 1872
Size: 6595 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2983 3873 5439 5957 6428 7339
Size: 197 926 1040 890 1566 518 471 911
Cuts at: 7339 9244 10145 10407 820
Size: 1905 901 262 1553
Fragments arranged by size:
1905 1566 1553 1040 926 911 901 890
518 471 262 197
SnaBI TAC'GTA
Cuts at: 493 8931 493
Size: 8438 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11140
SphI G_CATG'C
Cuts at: 1132 8498 1132
Size: 7366 3774
SspI AAT'ATT
Cuts at: 5 52 2188 2450 2756 6070 9064 5
Size: 47 2136 262 306 3314 2994 2081
Fragments arranged by size:
3314 2994 2136 2081 306 262 47
StuI AGG'CCT
Cuts at: 1517 5426 6456 8003 1517
Size: 3909 1030 1547 4654
Fragments arranged by size:
4654 3909 1547 1030
StyI C'CwwG_G
Cuts at: 513 1065 2004 3138 3441 3579 4073 8272 8358
Size: 552 939 1134 303 138 494 4199 86
Cuts at: 8358 8579 513
Size: 221 3074
Fragments arranged by size:
4199 3074 1134 939 552 494 303 221
138 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3435 6629 6710 7110 9167 9184 9337
Size: 1255 1855 3194 81 400 2057 17 153
Cuts at: 9337 9522 9967 325
Size: 185 445 1498
Fragments arranged by size:
3194 2057 1855 1498 1255 445 400 185
153 81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5209 5740 5807
Size: 274 80 33 51 1443 3232 531 67
Cuts at: 5807 9386 11098 96
Size: 3579 1712 138
Fragments arranged by size:
3579 3232 1712 1443 531 274 138 80
67 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 3311 3343 11100 11132 2615
Size: 32 664 32 7757 32 2623
Fragments arranged by size:
7757 2623 664 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11140
XbaI T'CTAG_A
Cuts at: 8476 8476
Size: 11140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3439 4018 5075 3439
Size: 579 1057 9504
Fragments arranged by size:
9504 1057 579
XhoI C'TCGA_G
Cuts at: 1943 2983 3873 1943
Size: 1040 890 9210
Fragments arranged by size:
9210 1040 890
XmaI C'CCGG_G
Cuts at: 1870 8465 1870
Size: 6595 4545
XmnI GAAnn'nnTTC
Cuts at: 3427 9269 3427
Size: 5842 5298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2074 5308 8948 276
Size: 53 83 186 936 540 3234 3640 2468
Fragments arranged by size:
3640 3234 2468 936 540 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BsmBI BstZ17I FseI FspAI
NaeI NgoMIV NruI PacI RsrII SanDI SrfI SwaI