(Circular) (Six-base) MAPSORT of: pFN21AE2363.seq Check: 7393 from: 1 to: 6256 pFN21AE2363 6256 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 3443 2729 Size: 714 5542 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 3599 1937 Size: 418 1244 4594 Fragments arranged by size: 4594 1244 418 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 4319 5703 5971 1632 Size: 600 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 600 268 AflII C'TTAA_G Cuts at: 820 1017 3500 820 Size: 197 2483 3576 Fragments arranged by size: 3576 2483 197 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 2786 5087 2786 Size: 2301 3955 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 2798 1166 Size: 1632 4624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3274 3999 5571 3274 Size: 725 1572 3959 Fragments arranged by size: 3959 1572 725 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2253 2369 3011 3354 3581 1870 Size: 73 310 116 642 343 227 4545 Fragments arranged by size: 4545 642 343 310 227 116 73 AvrII C'CTAG_G Cuts at: 3115 3115 Size: 6256 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2283 3577 5032 618 Size: 325 217 627 233 263 1294 1455 1842 Fragments arranged by size: 1842 1455 1294 627 325 263 233 217 BanII G_rGCy'C Cuts at: 1391 2529 2876 3575 1391 Size: 1138 347 699 4072 Fragments arranged by size: 4072 1138 699 347 BbeI G_GCGC'C Cuts at: 2287 2287 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 928 Size: 971 266 5019 Fragments arranged by size: 5019 971 266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4447 4481 1903 Size: 34 50 34 2426 34 3678 Fragments arranged by size: 3678 2426 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 4867 136 Size: 107 122 71 631 605 371 2824 1525 Fragments arranged by size: 2824 1525 631 605 371 122 107 71 BglII A'GATC_T Cuts at: 3252 6251 3252 Size: 2999 3257 BlpI GC'TnA_GC Cuts at: 2033 3673 2033 Size: 1640 4616 Bme1580I G_kGCm'C Cuts at: 1790 2205 4317 5473 1790 Size: 415 2112 1156 2573 Fragments arranged by size: 2573 2112 1156 415 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3987 4945 442 Size: 416 53 83 399 24 2570 958 1753 Fragments arranged by size: 2570 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2876 3377 4916 1239 Size: 785 852 501 1539 2579 Fragments arranged by size: 2579 1539 852 785 501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 4381 5246 5544 2825 Size: 1556 865 298 3537 Fragments arranged by size: 3537 1556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2366 4919 882 Size: 617 867 2553 2219 Fragments arranged by size: 2553 2219 867 617 BsaAI yAC'GTr Cuts at: 493 1204 2206 4047 493 Size: 711 1002 1841 2702 Fragments arranged by size: 2702 1841 1002 711 BsaBI GATnn'nnATC Cuts at: 2232 6250 2232 Size: 4018 2238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 4063 4445 5080 Size: 53 83 186 936 751 1779 382 635 Cuts at: 5080 275 Size: 1451 Fragments arranged by size: 1779 1451 936 751 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2786 4689 5087 5361 5508 5919 2786 Size: 1903 398 274 147 411 3123 Fragments arranged by size: 3123 1903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2053 3146 3263 3311 1186 Size: 867 1093 117 48 4131 Fragments arranged by size: 4131 1093 867 117 48 BseYI C'CCAG_C Cuts at: 2457 2754 2860 2876 3072 3484 5459 5955 2457 Size: 297 106 16 196 412 1975 496 2758 Fragments arranged by size: 2758 1975 496 412 297 196 106 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3242 3287 1163 Size: 573 1506 45 4132 Fragments arranged by size: 4132 1506 573 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4317 4402 5473 4317 Size: 85 1071 5100 Fragments arranged by size: 5100 1071 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2529 2876 3575 4317 4402 5473 Size: 399 415 324 347 699 742 85 1071 Cuts at: 5473 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 699 415 399 347 324 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 3443 3611 844 Size: 1885 714 168 3489 Fragments arranged by size: 3489 1885 714 168 BsrBI CCG'CTC Cuts at: 1394 1823 2305 2344 4143 1394 Size: 429 482 39 1799 3507 Fragments arranged by size: 3507 1799 482 429 39 BsrDI GCAATG_nn' Cuts at: 65 1248 4751 4925 65 Size: 1183 3503 174 1396 Fragments arranged by size: 3503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2310 2786 4900 5087 1700 Size: 110 500 476 2114 187 2869 Fragments arranged by size: 2869 2114 500 476 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2216 3526 1184 Size: 1032 1310 3914 Fragments arranged by size: 3914 1310 1032 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2800 2491 Size: 309 5947 BstYI r'GATC_y Cuts at: 1972 3252 3358 3586 3953 4338 4355 5807 5893 Size: 1280 106 228 367 385 17 1452 86 Cuts at: 5893 5905 6251 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1280 385 367 346 228 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3084 3084 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2279 2490 2555 2913 3021 513 Size: 552 1214 211 65 358 108 3748 Fragments arranged by size: 3748 1214 552 358 211 108 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 487 Size: 683 56 1508 4009 Fragments arranged by size: 4009 1508 683 56 BtsI GCAGTG_nn' Cuts at: 2927 3772 4566 4586 2927 Size: 845 794 20 4597 Fragments arranged by size: 4597 845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2497 2542 2745 Size: 54 1425 50 275 18 667 45 203 Cuts at: 2745 2889 3133 3703 4592 6221 8 Size: 144 244 570 889 1629 43 Fragments arranged by size: 1629 1425 889 667 570 275 244 203 144 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4186 1353 Size: 2833 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 3180 4843 5217 5363 387 Size: 1064 138 666 925 1663 374 146 1280 Fragments arranged by size: 1663 1280 1064 925 666 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2232 2876 3377 4319 4916 5703 Size: 393 392 208 644 501 942 597 787 Cuts at: 5703 5971 1239 Size: 268 1524 Fragments arranged by size: 1524 942 787 644 597 501 393 392 268 208 EcoNI CCTnn'n_nnAGG Cuts at: 2678 2678 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2274 3080 2274 Size: 806 5450 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2287 5403 1678 Size: 609 3116 2531 Fragments arranged by size: 3116 2531 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373 Size: 32 135 32 3325 32 42 32 2626 Fragments arranged by size: 3325 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2770 3600 3846 669 Size: 988 281 238 594 830 246 3079 Fragments arranged by size: 3079 988 830 594 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2283 2283 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2564 2859 5370 5554 1305 Size: 1259 295 2511 184 2007 Fragments arranged by size: 2511 2007 1259 295 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2499 2544 3135 10 Size: 54 1425 343 667 45 591 3131 Fragments arranged by size: 3131 1425 667 591 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2435 2489 2763 2798 4214 4573 4732 Size: 648 1269 54 274 35 1416 359 159 Cuts at: 4732 518 Size: 2042 Fragments arranged by size: 2042 1416 1269 648 359 274 159 54 35 MspA1I CmG'CkG Cuts at: 1606 2119 2825 3672 4349 5497 5742 1606 Size: 513 706 847 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 706 677 513 245 NaeI GCC'GGC Cuts at: 2312 2312 Size: 6256 NarI GG'CG_CC Cuts at: 2284 2284 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 2279 2490 513 Size: 552 1214 211 4279 Fragments arranged by size: 4279 1214 552 211 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2310 2310 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2912 3614 5159 1132 Size: 97 1683 702 1545 2229 Fragments arranged by size: 2229 1683 1545 702 97 PasI CC'CwG_GG Cuts at: 1348 2699 1348 Size: 1351 4905 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2761 1108 Size: 1653 4603 PfoI T'CCnGG_A Cuts at: 2972 2972 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PmlI CAC'GTG Cuts at: 2206 2206 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2274 2274 Size: 6256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 3608 830 Size: 2778 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2119 2825 2119 Size: 706 5550 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 2274 2274 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SexAI A'CCwGG_T Cuts at: 3323 3323 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 3604 4739 5420 5611 826 Size: 219 2559 1135 681 191 1471 Fragments arranged by size: 2559 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2285 2285 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2255 3356 3583 1872 Size: 383 1101 227 4545 Fragments arranged by size: 4545 1101 383 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 2840 3011 3500 4360 5261 Size: 197 926 426 471 171 489 860 901 Cuts at: 5261 5523 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 860 489 471 426 262 197 171 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2912 3614 1132 Size: 1780 702 3774 Fragments arranged by size: 3774 1780 702 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 3506 1517 Size: 1989 4267 StyI C'CwwG_G Cuts at: 513 1065 2279 2376 2412 2490 2568 3115 3695 Size: 552 1214 97 36 78 78 547 580 Cuts at: 3695 513 Size: 3074 Fragments arranged by size: 3074 1214 580 552 547 97 78 78 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2852 3086 3398 3476 4283 4300 4453 Size: 1255 1272 234 312 78 807 17 153 Cuts at: 4453 4638 5083 325 Size: 185 445 1498 Fragments arranged by size: 1498 1272 1255 807 445 312 234 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3375 4502 6214 Size: 274 80 33 51 1443 1398 1127 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 1712 1443 1398 1127 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6216 6248 6216 Size: 32 6224 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2822 2884 3331 3446 2822 Size: 62 447 115 5632 Fragments arranged by size: 5632 447 115 62 XhoI C'TCGA_G Cuts at: 1943 2369 3011 1943 Size: 426 642 5188 Fragments arranged by size: 5188 642 426 XmaI C'CCGG_G Cuts at: 1870 2253 3354 3581 1870 Size: 383 1101 227 4545 Fragments arranged by size: 4545 1101 383 227 XmnI GAAnn'nnTTC Cuts at: 2162 2393 4385 2162 Size: 231 1992 4033 Fragments arranged by size: 4033 1992 231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI