Restriction map for
pFN21AE2361
(Circular) (Six-base) MAPSORT of: pFN21AE2361.seq Check: 1575 from: 1 to: 5497
pFN21AE2361 5497 bp
With 182 enzymes: SgfI *
July 22, 2011 10:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2564 2622 3560 4944 5212 1632
Size: 842 90 58 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 842 268 90 58
AfeI AGC'GCT
Cuts at: 2226 2226
Size: 5497
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2160 2771 1184
Size: 976 611 3910
Fragments arranged by size:
3910 976 611
AlwNI CAG_nnn'CTG
Cuts at: 2626 2785 3240 4812 2626
Size: 159 455 1572 3311
Fragments arranged by size:
3311 1572 455 159
ApaI G_GGCC'C
Cuts at: 2594 2594
Size: 5497
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 1870
Size: 73 879 4545
Fragments arranged by size:
4545 879 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2126 2747 2818 4273
Size: 325 217 627 249 90 621 71 1455
Cuts at: 4273 618
Size: 1842
Fragments arranged by size:
1842 1455 627 621 325 249 217 90
71
BanII G_rGCy'C
Cuts at: 1391 2594 2816 1391
Size: 1203 222 4072
Fragments arranged by size:
4072 1203 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2098 2107 2292
Size: 107 122 71 631 605 426 9 185
Cuts at: 2292 2561 4108 136
Size: 269 1547 1525
Fragments arranged by size:
1547 1525 631 605 426 269 185 122
107 71 9
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2016 2914 2016
Size: 898 4599
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2594 2750 3558 4714 1790
Size: 287 517 156 808 1156 2573
Fragments arranged by size:
2573 1156 808 517 287 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4186 442
Size: 416 53 83 399 24 1811 958 1753
Fragments arranged by size:
1811 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 4157 1239
Size: 803 2115 2579
Fragments arranged by size:
2579 2115 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2205 3622 4487 4785 2205
Size: 1417 865 298 2917
Fragments arranged by size:
2917 1417 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 4160 882
Size: 617 580 2081 2219
Fragments arranged by size:
2219 2081 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5497
BseYI C'CCAG_C
Cuts at: 4700 5196 4700
Size: 496 5001
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2386 1163
Size: 573 650 4274
Fragments arranged by size:
4274 650 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2721 3558 3643 4714 2721
Size: 837 85 1071 3504
Fragments arranged by size:
3504 1071 837 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2594 2721 2750 2816 3558 3643
Size: 399 287 517 127 29 66 742 85
Cuts at: 3643 4714 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 287 127 85
66 29
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 2392 3386 5116 2392
Size: 994 1730 2773
Fragments arranged by size:
2773 1730 994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2428 2852 844
Size: 1584 424 3489
Fragments arranged by size:
3489 1584 424
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 2192 3384 1394
Size: 429 211 158 1192 3507
Fragments arranged by size:
3507 1192 429 211 158
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 3992 4166 65
Size: 1183 1362 1382 174 1396
Fragments arranged by size:
1396 1382 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 2506 2582 1184
Size: 1322 76 4099
Fragments arranged by size:
4099 1322 76
BstYI r'GATC_y
Cuts at: 1972 2622 2827 3194 3579 3596 5048 5134 5146
Size: 650 205 367 385 17 1452 86 12
Cuts at: 5146 5492 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 650 385 367 346 205 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 5497
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4006
Fragments arranged by size:
4006 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 2484 3013 3807 3827 2484
Size: 529 794 20 4154
Fragments arranged by size:
4154 794 529 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2210 2245 529
Size: 35 1646 35 3781
Fragments arranged by size:
3781 1646 35 35
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2365 2562 2647
Size: 54 1425 50 275 18 535 197 85
Cuts at: 2647 2944 3833 5462 8
Size: 297 889 1629 43
Fragments arranged by size:
1629 1425 889 535 297 275 197 85
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2598 3427 1353
Size: 1245 829 3423
Fragments arranged by size:
3423 1245 829
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2474 2564 2622 3560 4157 4944
Size: 393 410 432 90 58 938 597 787
Cuts at: 4944 5212 1239
Size: 268 1524
Fragments arranged by size:
1524 938 787 597 432 410 393 268
90 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2121 2121
Size: 5497
EcoO109I rG'GnC_Cy
Cuts at: 2449 2591 2449
Size: 142 5355
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2016 2228 2792 4644 1678
Size: 338 212 564 1852 2531
Fragments arranged by size:
2531 1852 564 338 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373
Size: 32 135 32 2566 32 42 32 2626
Fragments arranged by size:
2626 2566 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 2943
Fragments arranged by size:
2943 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3455 3814 3973 518
Size: 648 2289 359 159 2042
Fragments arranged by size:
2289 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2913 3590 4738 4983 1606
Size: 414 893 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4006
Fragments arranged by size:
4006 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PspOMI G'GGCC_C
Cuts at: 2590 2590
Size: 5497
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 5465
PstI C_TGCA'G
Cuts at: 830 2149 2849 830
Size: 1319 700 3478
Fragments arranged by size:
3478 1319 700
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
PvuII CAG'CTG
Cuts at: 2020 2020
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2145 2275 2308 2845 3980 4661 4852
Size: 219 1100 130 33 537 1135 681 191
Cuts at: 4852 826
Size: 1471
Fragments arranged by size:
1471 1135 1100 681 537 219 191 130
33
SfiI GGCCn_nnn'nGGCC
Cuts at: 2107 2561 2107
Size: 454 5043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2220 3601 4502 4764 820
Size: 197 926 277 1381 901 262 1553
Fragments arranged by size:
1553 1381 926 901 277 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 2936 513
Size: 552 939 495 437 3074
Fragments arranged by size:
3074 939 552 495 437
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2052 3524 3541 3694 3879 4324 325
Size: 1255 472 1472 17 153 185 445 1498
Fragments arranged by size:
1498 1472 1255 472 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2500 2532 5457 5489 2500
Size: 32 2925 32 2508
Fragments arranged by size:
2925 2508 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 2623
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI