Restriction map for
pFN21AE2356
(Circular) (Six-base) MAPSORT of: pFN21AE2356.seq Check: 6986 from: 1 to: 12880
pFN21AE2356 12880 bp
With 182 enzymes: SgfI *
July 22, 2011 10:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6427 7751 6427
Size: 1324 11556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6261 10690 278
Size: 53 83 186 936 4725 4429 2468
Fragments arranged by size:
4725 4429 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2940 10223 1937
Size: 1003 7283 4594
Fragments arranged by size:
7283 4594 1003
Acc65I G'GTAC_C
Cuts at: 7103 10201 7103
Size: 3098 9782
AclI AA'CG_TT
Cuts at: 1563 11007 11380 1563
Size: 9444 373 3063
Fragments arranged by size:
9444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 2976 4130 7260 7925 8003 10943 12327
Size: 773 571 1154 3130 665 78 2940 1384
Cuts at: 12327 12595 1632
Size: 268 1917
Fragments arranged by size:
3130 2940 1917 1384 1154 773 665 571
268 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12683
AflIII A'CryG_T
Cuts at: 1203 2301 7896 11779 12756 1203
Size: 1098 5595 3883 977 1327
Fragments arranged by size:
5595 3883 1327 1098 977
AgeI A'CCGG_T
Cuts at: 9563 11711 9563
Size: 2148 10732
AhdI GACnn_n'nnGTC
Cuts at: 11609 11609
Size: 12880
AleI CACnn'nnGTG
Cuts at: 1166 3803 4646 5912 6461 7895 1166
Size: 2637 843 1266 549 1434 6151
Fragments arranged by size:
6151 2637 1434 1266 843 549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2894 7808 1184
Size: 1710 4914 6256
Fragments arranged by size:
6256 4914 1710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 12848
AlwNI CAG_nnn'CTG
Cuts at: 2497 4448 5777 6530 8463 9015 10623 12195 2497
Size: 1951 1329 753 1933 552 1608 1572 3182
Fragments arranged by size:
3182 1951 1933 1608 1572 1329 753 552
ApaI G_GGCC'C
Cuts at: 3837 4439 4628 5109 5309 6382 6455 6540 6971
Size: 602 189 481 200 1073 73 85 431
Cuts at: 6971 7700 8859 9932 3837
Size: 729 1159 1073 6785
Fragments arranged by size:
6785 1159 1073 1073 729 602 481 431
200 189 85 73
ApaLI G'TGCA_C
Cuts at: 3923 9335 10019 10937 12093 3923
Size: 5412 684 918 1156 4710
Fragments arranged by size:
5412 4710 1156 918 684
ApoI r'AATT_y
Cuts at: 1493 5714 10189 10415 10567 12676 1493
Size: 4221 4475 226 152 2109 1697
Fragments arranged by size:
4475 4221 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 11434 160
Size: 872 10402 1606
Fragments arranged by size:
10402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12880
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2315 2324 2658 4295 5212 5467
Size: 73 282 90 9 334 1637 917 255
Cuts at: 5467 5890 6381 6746 7130 7420 7816 7957 9021
Size: 423 491 365 384 290 396 141 1064
Cuts at: 9021 9312 9330 9346 9548 9607 9924 10205 1870
Size: 291 18 16 202 59 317 281 4545
Fragments arranged by size:
4545 1637 1064 917 491 423 396 384
365 334 317 291 290 282 281 255
202 141 90 73 59 18 16 9
BamHI G'GATC_C
Cuts at: 2508 8699 10210 10577 2508
Size: 6191 1511 367 4811
Fragments arranged by size:
6191 4811 1511 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 2579 2695 2798 3371
Size: 325 217 627 734 58 116 103 573
Cuts at: 3371 3512 3625 3797 4175 4885 5143 5237 5633
Size: 141 113 172 378 710 258 94 396
Cuts at: 5633 5669 5933 6140 6427 6500 6574 6956 7103
Size: 36 264 207 287 73 74 382 147
Cuts at: 7103 7283 7321 7463 7739 7883 8321 8434 8942
Size: 180 38 142 276 144 438 113 508
Cuts at: 8942 9199 9308 9455 9995 10201 11656 618
Size: 257 109 147 540 206 1455 1842
Fragments arranged by size:
1842 1455 734 710 627 573 540 508
438 396 382 378 325 287 276 264
258 257 217 207 206 180 172 147
147 144 142 141 116 113 113 109
103 94 74 73 58 38 36
BanII G_rGCy'C
Cuts at: 1391 2208 3531 3704 3837 4262 4356 4439 4628
Size: 817 1323 173 133 425 94 83 189
Cuts at: 4628 4868 5109 5309 6020 6299 6382 6455 6540
Size: 240 241 200 711 279 83 73 85
Cuts at: 6540 6971 7074 7236 7398 7550 7700 7826 7974
Size: 431 103 162 162 152 150 126 148
Cuts at: 7974 8319 8859 9267 9549 9872 9932 10199 1391
Size: 345 540 408 282 323 60 267 4072
Fragments arranged by size:
4072 1323 817 711 540 431 425 408
345 323 282 279 267 241 240 200
189 173 162 162 152 150 148 133
126 103 94 85 83 83 73 60
BbeI G_GCGC'C
Cuts at: 2583 6431 7287 2583
Size: 3848 856 8176
Fragments arranged by size:
8176 3848 856
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4337 7433 928
Size: 971 2438 3096 6375
Fragments arranged by size:
6375 3096 2438 971
BbvCI CC'TCA_GC
Cuts at: 2195 4901 5412 7349 2195
Size: 2706 511 1937 7726
Fragments arranged by size:
7726 2706 1937 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2350 2384 5458 5492 6904 6938 11071
Size: 34 413 34 3074 34 1412 34 4133
Cuts at: 11071 11105 1903
Size: 34 3678
Fragments arranged by size:
4133 3678 3074 1412 413 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10772 11982 10772
Size: 1210 11670
BclI T'GATC_A
Cuts at: 1548 6999 1548
Size: 5451 7429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2663 3762 4700
Size: 107 122 71 631 605 991 1099 938
Cuts at: 4700 7999 8778 11491 136
Size: 3299 779 2713 1525
Fragments arranged by size:
3299 2713 1525 1099 991 938 779 631
605 122 107 71
BglII A'GATC_T
Cuts at: 6735 12875 6735
Size: 6140 6740
BlpI GC'TnA_GC
Cuts at: 2234 10297 2234
Size: 8063 4817
Bme1580I G_kGCm'C
Cuts at: 1790 2526 2700 2803 3030 3376 3560 3837 3927
Size: 736 174 103 227 346 184 277 90
Cuts at: 3927 4200 4254 4439 4628 5109 5309 5712 6012
Size: 273 54 185 189 481 200 403 300
Cuts at: 6012 6143 6382 6455 6540 6971 7700 7886 7954
Size: 131 239 73 85 431 729 186 68
Cuts at: 7954 8844 8859 8898 8947 9313 9339 9460 9932
Size: 890 15 39 49 366 26 121 472
Cuts at: 9932 10023 10941 12097 1790
Size: 91 918 1156 2573
Fragments arranged by size:
2573 1156 918 890 736 729 481 472
431 403 366 346 300 277 273 239
227 200 189 186 185 184 174 131
121 103 91 90 85 73 68 54
49 39 26 15
BmgBI CAC'GTC
Cuts at: 1318 3928 4399 7897 1318
Size: 2610 471 3498 6301
Fragments arranged by size:
6301 3498 2610 471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 2764 3824
Size: 416 53 83 399 24 1212 135 1060
Cuts at: 3824 6912 7309 10611 11569 442
Size: 3088 397 3302 958 1753
Fragments arranged by size:
3302 3088 1753 1212 1060 958 416 399
397 135 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3853 6190 7252 8758 3853
Size: 2337 1062 1506 7975
Fragments arranged by size:
7975 2337 1506 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 3308 4055 5069 5519 6104 6267 7686
Size: 1076 993 747 1014 450 585 163 1419
Cuts at: 7686 7794 8996 9236 9856 11540 1239
Size: 108 1202 240 620 1684 2579
Fragments arranged by size:
2579 1684 1419 1202 1076 1014 993 747
620 585 450 240 163 108
Bpu10I CC'TnA_GC
Cuts at: 2195 2976 4901 5412 6349 7349 7610 2195
Size: 781 1925 511 937 1000 261 7465
Fragments arranged by size:
7465 1925 1000 937 781 511 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3515 4325 5204 8462 9251 9844 11005 11870
Size: 1377 810 879 3258 789 593 1161 865
Cuts at: 11870 12168 2138
Size: 298 2850
Fragments arranged by size:
3258 2850 1377 1161 879 865 810 789
593 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 4098 5222 5773 6119 9157 11543
Size: 617 1113 1486 1124 551 346 3038 2386
Cuts at: 11543 882
Size: 2219
Fragments arranged by size:
3038 2386 2219 1486 1124 1113 617 551
346
BsaAI yAC'GTr
Cuts at: 493 1204 3654 4195 5866 6795 7198 8044 8359
Size: 711 2450 541 1671 929 403 846 315
Cuts at: 8359 8893 10054 10671 493
Size: 534 1161 617 2702
Fragments arranged by size:
2702 2450 1671 1161 929 846 711 617
541 534 403 315
BsaBI GATnn'nnATC
Cuts at: 7514 12874 7514
Size: 5360 7520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2483 2580 4128
Size: 53 83 186 936 498 452 97 1548
Cuts at: 4128 5760 6258 6428 7284 8124 9114 9426 10687
Size: 1632 498 170 856 840 990 312 1261
Cuts at: 10687 11069 11704 275
Size: 382 635 1451
Fragments arranged by size:
1632 1548 1451 1261 990 936 856 840
635 498 498 452 382 312 186 170
97 83 53
BsaWI w'CCGG_w
Cuts at: 9563 9797 11313 11711 11985 12132 12543 9563
Size: 234 1516 398 274 147 411 9900
Fragments arranged by size:
9900 1516 411 398 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2288 2318 2417 2447 5975
Size: 30 165 30 885 30 99 30 3528
Cuts at: 5975 6005 9679 9709 1178
Size: 30 3674 30 4349
Fragments arranged by size:
4349 3674 3528 885 165 99 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2984 3578 5114 5978 7241 7244 7260
Size: 1546 252 594 1536 864 1263 3 16
Cuts at: 7260 7325 7982 8642 9374 9847 9889 10058 1186
Size: 65 657 660 732 473 42 169 4008
Fragments arranged by size:
4008 1546 1536 1263 864 732 660 657
594 473 252 169 65 42 16 3
BseYI C'CCAG_C
Cuts at: 2526 3038 3587 6361 6448 6523 6607 8929 9035
Size: 512 549 2774 87 75 84 2322 106
Cuts at: 9035 9509 9744 12083 12579 2526
Size: 474 235 2339 496 2827
Fragments arranged by size:
2827 2774 2339 2322 549 512 496 474
235 106 87 84 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 2924 3301 4110 4736 5756 5966
Size: 573 669 519 377 809 626 1020 210
Cuts at: 5966 6202 7403 7487 7729 8742 9317 9668 1163
Size: 236 1201 84 242 1013 575 351 4375
Fragments arranged by size:
4375 1201 1020 1013 809 669 626 575
573 519 377 351 242 236 210 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5748 8868 10330 11091 12119 1540
Size: 275 186 3747 3120 1462 761 1028 2301
Fragments arranged by size:
3747 3120 2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2235 2337 3063 3531 3927 5883 6350 7074
Size: 84 102 726 468 396 1956 467 724
Cuts at: 7074 7176 7398 8043 9177 9339 10023 10941 11026
Size: 102 222 645 1134 162 684 918 85
Cuts at: 11026 12097 2151
Size: 1071 2934
Fragments arranged by size:
2934 1956 1134 1071 918 726 724 684
645 468 467 396 222 162 102 102
85 84
BsmI GAATG_Cn'
Cuts at: 2746 4958 9604 10391 10484 2746
Size: 2212 4646 787 93 5142
Fragments arranged by size:
5142 4646 2212 787 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6986 9121 9239 9251 9376 6986
Size: 2135 118 12 125 10490
Fragments arranged by size:
10490 2135 125 118 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2208 2235 2337 2526 2700 2803
Size: 399 361 57 27 102 189 174 103
Cuts at: 2803 3030 3063 3376 3531 3560 3704 3837 3927
Size: 227 33 313 155 29 144 133 90
Cuts at: 3927 4200 4254 4262 4356 4439 4628 4868 5109
Size: 273 54 8 94 83 189 240 241
Cuts at: 5109 5309 5712 5883 6012 6020 6143 6299 6350
Size: 200 403 171 129 8 123 156 51
Cuts at: 6350 6382 6455 6540 6971 7074 7176 7236 7398
Size: 32 73 85 431 103 102 60 162
Cuts at: 7398 7550 7700 7826 7886 7954 7974 8043 8319
Size: 152 150 126 60 68 20 69 276
Cuts at: 8319 8844 8859 8898 8947 9177 9267 9313 9339
Size: 525 15 39 49 230 90 46 26
Cuts at: 9339 9460 9549 9872 9932 10023 10199 10941 11026
Size: 121 89 323 60 91 176 742 85
Cuts at: 11026 12097 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 525 431 403 399 361
323 313 276 273 241 240 230 227
200 189 189 176 174 171 162 156
155 152 150 144 133 129 126 123
121 103 103 102 102 94 91 90
90 89 85 85 83 73 69 68
60 60 60 57 54 51 49 46
39 33 32 29 27 26 20 15
8 8
BspEI T'CCGG_A
Cuts at: 9797 12543 9797
Size: 2746 10134
BspHI T'CATG_A
Cuts at: 6613 10769 12499 6613
Size: 4156 1730 6994
Fragments arranged by size:
6994 4156 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 3337 5376 6427 6819 7751 8201 10235
Size: 1346 1147 2039 1051 392 932 450 2034
Cuts at: 10235 844
Size: 3489
Fragments arranged by size:
3489 2039 2034 1346 1147 1051 932 450
392
BsrBI CCG'CTC
Cuts at: 1394 1823 4300 5066 6137 10767 1394
Size: 429 2477 766 1071 4630 3507
Fragments arranged by size:
4630 3507 2477 1071 766 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2706 3178 3966 8212 9048 9720
Size: 1183 901 557 472 788 4246 836 672
Cuts at: 9720 11375 11549 65
Size: 1655 174 1396
Fragments arranged by size:
4246 1655 1396 1183 901 836 788 672
557 472 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 3218 3261 3515 4043 4694 5028
Size: 110 223 1185 43 254 528 651 334
Cuts at: 5028 6828 7022 7998 8390 8990 9563 9602 11524
Size: 1800 194 976 392 600 573 39 1922
Cuts at: 11524 11711 1700
Size: 187 2869
Fragments arranged by size:
2869 1922 1800 1185 976 651 600 573
528 392 334 254 223 194 187 110
43 39
BsrGI T'GTAC_A
Cuts at: 96 10077 96
Size: 9981 2899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12880
BssSI C'ACGA_G
Cuts at: 2345 4086 4974 5874 7223 9416 10940 11952 2345
Size: 1741 888 900 1349 2193 1524 1012 3273
Fragments arranged by size:
3273 2193 1741 1524 1349 1012 900 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 4416 5707 794
Size: 1703 1919 1291 7967
Fragments arranged by size:
7967 1919 1703 1291
BstBI TT'CG_AA
Cuts at: 11684 11684
Size: 12880
BstEII G'GTnAC_C
Cuts at: 1184 3052 3643 5135 5809 8386 8764 1184
Size: 1868 591 1492 674 2577 378 5300
Fragments arranged by size:
5300 2577 1868 1492 674 591 378
BstXI CCAn_nnnn'nTGG
Cuts at: 5339 5426 6463 7541 8493 9055 9422 9467 9832
Size: 87 1037 1078 952 562 367 45 365
Cuts at: 9832 9916 5339
Size: 84 8303
Fragments arranged by size:
8303 1078 1037 952 562 367 365 87
84 45
BstYI r'GATC_y
Cuts at: 1972 2112 2508 4557 5577 5837 6735 6864 8010
Size: 140 396 2049 1020 260 898 129 1146
Cuts at: 8010 8094 8481 8699 9483 10210 10577 10962 10979
Size: 84 387 218 784 727 367 385 17
Cuts at: 10979 12431 12517 12529 12875 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2049 1977 1452 1146 1020 898 784 727
396 387 385 367 346 260 218 140
129 86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2715 9672 2715
Size: 6957 5923
BtgI C'CryG_G
Cuts at: 513 1065 2073 2102 2307 2646 3145 3333 3900
Size: 552 1008 29 205 339 499 188 567
Cuts at: 3900 5373 6222 6946 7615 7689 7890 8881 9699
Size: 1473 849 724 669 74 201 991 818
Cuts at: 9699 9825 9975 513
Size: 126 150 3418
Fragments arranged by size:
3418 1473 1008 991 849 818 724 669
567 552 499 339 205 201 188 150
126 74 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2064 2200 2824 3243 3897 8428
Size: 683 56 838 136 624 419 654 4531
Cuts at: 8428 487
Size: 4939
Fragments arranged by size:
4939 4531 838 683 654 624 419 136
56
BtsI GCAGTG_nn'
Cuts at: 3207 4833 10396 11190 11210 3207
Size: 1626 5563 794 20 4877
Fragments arranged by size:
5563 4877 1626 794 20
ClaI AT'CG_AT
Cuts at: 1554 9438 1554
Size: 7884 4996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3178 3213 8235 8270 8863 8898 9718
Size: 35 2614 35 5022 35 593 35 820
Cuts at: 9718 9753 529
Size: 35 3656
Fragments arranged by size:
5022 3656 2614 820 593 35 35 35
35 35
DraI TTT'AAA
Cuts at: 10184 10184
Size: 12880
DraIII CAC_nnn'GTG
Cuts at: 3052 5143 5795 8047 8953 9754 3052
Size: 2091 652 2252 906 801 6178
Fragments arranged by size:
6178 2252 2091 906 801 652
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2764 6257 9149 10145 11887 809
Size: 507 1448 3493 2892 996 1742 1802
Fragments arranged by size:
3493 2892 1802 1742 1448 996 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 3177 3754
Size: 54 1425 50 275 18 1240 107 577
Cuts at: 3754 6421 6465 6826 7686 8884 8988 10327 11216
Size: 2667 44 361 860 1198 104 1339 889
Cuts at: 11216 12845 8
Size: 1629 43
Fragments arranged by size:
2667 1629 1425 1339 1240 1198 889 860
577 361 275 107 104 54 50 44
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10327 1537
Size: 275 8515 4090
Fragments arranged by size:
8515 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 10810 1353
Size: 9457 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 2270 5576 8440 11467 11841
Size: 1064 138 516 165 3306 2864 3027 374
Cuts at: 11841 11987 387
Size: 146 1280
Fragments arranged by size:
3306 3027 2864 1280 1064 516 374 165
146 138
EcoICRI GAG'CTC
Cuts at: 3529 7072 7396 3529
Size: 3543 324 9013
Fragments arranged by size:
9013 3543 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2405 2976 3308 4055 4130 5069
Size: 393 683 90 571 332 747 75 939
Cuts at: 5069 5519 6104 6267 7260 7686 7794 7925 8003
Size: 450 585 163 993 426 108 131 78
Cuts at: 8003 8996 9236 9856 10943 11540 12327 12595 1239
Size: 993 240 620 1087 597 787 268 1524
Fragments arranged by size:
1524 1087 993 993 939 787 747 683
620 597 585 571 450 426 393 332
268 240 163 131 108 90 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 5804 2090
Size: 3714 9166
EcoO109I rG'GnC_Cy
Cuts at: 2083 2632 2950 3737 3833 3834 4436 4624 4625
Size: 549 318 787 96 1 602 188 1
Cuts at: 4625 4849 4921 5105 5106 5305 5306 5774 5986
Size: 224 72 184 1 199 1 468 212
Cuts at: 5986 6148 6170 6378 6452 6537 6601 6968 7353
Size: 162 22 208 74 85 64 367 385
Cuts at: 7353 7696 7863 8650 8855 8856 9040 9062 9514
Size: 343 167 787 205 1 184 22 452
Cuts at: 9514 9612 9928 9929 10174 2083
Size: 98 316 1 245 4789
Fragments arranged by size:
4789 787 787 602 549 468 452 385
367 343 318 316 245 224 212 208
205 199 188 184 184 167 162 98
96 85 74 72 64 22 22 1
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 10189 10567 10189
Size: 378 12502
EcoRV GAT'ATC
Cuts at: 5122 5122
Size: 12880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4279 4885 6037 9985 4279
Size: 606 1152 3948 7174
Fragments arranged by size:
7174 3948 1152 606
FspI TGC'GCA
Cuts at: 8057 11386 8057
Size: 3329 9551
HaeII r_GCGC'y
Cuts at: 1678 2173 2583 6431 7130 7287 7990 8524 8695
Size: 495 410 3848 699 157 703 534 171
Cuts at: 8695 8823 9036 12027 1678
Size: 128 213 2991 2531
Fragments arranged by size:
3848 2991 2531 703 699 534 495 410
213 171 157 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2288 2320 5567
Size: 32 135 32 482 32 202 32 3247
Cuts at: 5567 5599 5822 5854 7685 7717 9623 9655 11521
Size: 32 223 32 1831 32 1906 32 1866
Cuts at: 11521 11553 11595 11627 1373
Size: 32 42 32 2626
Fragments arranged by size:
3247 2626 1906 1866 1831 482 223 202
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6100 6262 7627 10224 10470 669
Size: 988 281 4162 162 1365 2597 246 3079
Fragments arranged by size:
4162 3079 2597 1365 988 281 246 162
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12880
HpaI GTT'AAC
Cuts at: 10470 10470
Size: 12880
KasI G'GCGC_C
Cuts at: 2579 6427 7283 2579
Size: 3848 856 8176
Fragments arranged by size:
8176 3848 856
KpnI G_GTAC'C
Cuts at: 7107 10205 7107
Size: 3098 9782
MfeI C'AATT_G
Cuts at: 10479 10479
Size: 12880
MluI A'CGCG_T
Cuts at: 12756 12756
Size: 12880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3390 5565 11994 12178 1305
Size: 2085 2175 6429 184 2007
Fragments arranged by size:
6429 2175 2085 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3072 3179 3756 8886 10
Size: 54 1425 343 1240 107 577 5130 4004
Fragments arranged by size:
5130 4004 1425 1240 577 343 107 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2441 3803 4154 4532 4535 4583
Size: 648 1269 6 1362 351 378 3 48
Cuts at: 4583 4646 4826 5912 6461 7472 7590 7790 7895
Size: 63 180 1086 549 1011 118 200 105
Cuts at: 7895 9053 9420 10001 10007 10838 11197 11356 518
Size: 1158 367 581 6 831 359 159 2042
Fragments arranged by size:
2042 1362 1269 1158 1086 1011 831 648
581 549 378 367 359 351 200 180
159 118 105 63 48 6 6 3
MspA1I CmG'CkG
Cuts at: 1606 2019 2494 2530 2821 3315 3522 4067 4094
Size: 413 475 36 291 494 207 545 27
Cuts at: 4094 4185 4805 4894 6639 7252 7873 7933 8929
Size: 91 620 89 1745 613 621 60 996
Cuts at: 8929 9301 9652 10296 10973 12121 12366 1606
Size: 372 351 644 677 1148 245 2120
Fragments arranged by size:
2120 1745 1148 996 677 644 621 620
613 545 494 475 413 372 351 291
245 207 91 89 60 36 27
NaeI GCC'GGC
Cuts at: 2035 3220 3263 3517 4696 5030 8992 9604 2035
Size: 1185 43 254 1179 334 3962 612 5311
Fragments arranged by size:
5311 3962 1185 1179 612 334 254 43
NarI GG'CG_CC
Cuts at: 2580 6428 7284 2580
Size: 3848 856 8176
Fragments arranged by size:
8176 3848 856
NcoI C'CATG_G
Cuts at: 513 1065 3333 6946 7615 9699 513
Size: 552 2268 3613 669 2084 3694
Fragments arranged by size:
3694 3613 2268 2084 669 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12880
NgoMIV G'CCGG_C
Cuts at: 2033 3218 3261 3515 4694 5028 8990 9602 2033
Size: 1185 43 254 1179 334 3962 612 5311
Fragments arranged by size:
5311 3962 1185 1179 612 334 254 43
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12880
NotI GC'GGCC_GC
Cuts at: 10327 10327
Size: 12880
NspI r_CATG'y
Cuts at: 1132 1229 2251 3359 5092 7044 8075 8292 10238
Size: 97 1022 1108 1733 1952 1031 217 1946
Cuts at: 10238 11783 1132
Size: 1545 2229
Fragments arranged by size:
2229 1952 1946 1733 1545 1108 1031 1022
217 97
PasI CC'CwG_GG
Cuts at: 1348 3118 4628 6290 6332 7541 9932 1348
Size: 1770 1510 1662 42 1209 2391 4296
Fragments arranged by size:
4296 2391 1770 1662 1510 1209 42
PciI A'CATG_T
Cuts at: 11779 11779
Size: 12880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 3654 5060 6739 6952 7201 7299 7621
Size: 1539 1007 1406 1679 213 249 98 322
Cuts at: 7621 8851 1108
Size: 1230 5137
Fragments arranged by size:
5137 1679 1539 1406 1230 1007 322 249
213 98
PfoI T'CCnGG_A
Cuts at: 6244 6774 9129 9252 6244
Size: 530 2355 123 9872
Fragments arranged by size:
9872 2355 530 123
PmeI GTTT'AAAC
Cuts at: 10184 10184
Size: 12880
PmlI CAC'GTG
Cuts at: 3654 5866 7198 8044 8359 8893 3654
Size: 2212 1332 846 315 534 7641
Fragments arranged by size:
7641 2212 1332 846 534 315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10949 10981 12462 12494 10949
Size: 32 1481 32 11335
Fragments arranged by size:
11335 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2632 2950 3737 4849 5986 6170 6601 7863
Size: 549 318 787 1112 1137 184 431 1262
Cuts at: 7863 8650 9062 9514 10174 2083
Size: 787 412 452 660 4789
Fragments arranged by size:
4789 1262 1137 1112 787 787 660 549
452 431 412 318 184
PshAI GACnn'nnGTC
Cuts at: 1613 4137 6411 1613
Size: 2524 2274 8082
Fragments arranged by size:
8082 2524 2274
PsiI TTA'TAA
Cuts at: 10450 10450
Size: 12880
PspOMI G'GGCC_C
Cuts at: 3833 4435 4624 5105 5305 6378 6451 6536 6967
Size: 602 189 481 200 1073 73 85 431
Cuts at: 6967 7696 8855 9928 3833
Size: 729 1159 1073 6785
Fragments arranged by size:
6785 1159 1073 1073 729 602 481 431
200 189 85 73
PspXI vC'TCGA_Gb
Cuts at: 2225 2315 2324 9548 2225
Size: 90 9 7224 5557
Fragments arranged by size:
7224 5557 90 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2243 2275 10061 10093 2243
Size: 32 7786 32 5030
Fragments arranged by size:
7786 5030 32 32
PstI C_TGCA'G
Cuts at: 830 2187 2886 3459 4379 4444 6413 6438 9986
Size: 1357 699 573 920 65 1969 25 3548
Cuts at: 9986 10232 830
Size: 246 3478
Fragments arranged by size:
3548 3478 1969 1357 920 699 573 246
65 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12880
PvuII CAG'CTG
Cuts at: 2530 2821 4094 7252 7873 7933 9301 9652 2530
Size: 291 1273 3158 621 60 1368 351 5758
Fragments arranged by size:
5758 3158 1368 1273 621 351 291 60
SacI G_AGCT'C
Cuts at: 3531 7074 7398 3531
Size: 3543 324 9013
Fragments arranged by size:
9013 3543 324
SalI G'TCGA_C
Cuts at: 1936 10222 1936
Size: 8286 4594
SanDI GG'GwC_CC
Cuts at: 2632 4849 6170 8650 9514 2632
Size: 2217 1321 2480 864 5998
Fragments arranged by size:
5998 2480 2217 1321 864
SbfI CC_TGCA'GG
Cuts at: 6438 10232 6438
Size: 3794 9086
ScaI AGT'ACT
Cuts at: 3614 11128 12840 3614
Size: 7514 1712 3654
Fragments arranged by size:
7514 3654 1712
SexAI A'CCwGG_T
Cuts at: 2794 7362 9136 2794
Size: 4568 1774 6538
Fragments arranged by size:
6538 4568 1774
SfcI C'TryA_G
Cuts at: 826 1045 2183 2882 3455 4375 4440 5650 6409
Size: 219 1138 699 573 920 65 1210 759
Cuts at: 6409 6434 7780 8824 9877 9982 10228 11363 12044
Size: 25 1346 1044 1053 105 246 1135 681
Cuts at: 12044 12235 826
Size: 191 1471
Fragments arranged by size:
1471 1346 1210 1138 1135 1053 1044 920
759 699 681 573 246 219 191 105
65 25
SfoI GGC'GCC
Cuts at: 2581 6429 7285 2581
Size: 3848 856 8176
Fragments arranged by size:
8176 3848 856
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12880
SmaI CCC'GGG
Cuts at: 1872 2660 5214 6383 7818 9023 9314 9332 10207
Size: 788 2554 1169 1435 1205 291 18 875
Cuts at: 10207 1872
Size: 4545
Fragments arranged by size:
4545 2554 1435 1205 1169 875 788 291
18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2225 2315 2324 3530 4340
Size: 197 926 210 72 90 9 1206 810
Cuts at: 4340 5219 8477 9266 9548 9859 10984 11885 12147
Size: 879 3258 789 282 311 1125 901 262
Cuts at: 12147 820
Size: 1553
Fragments arranged by size:
3258 1553 1206 1125 926 901 879 810
789 311 282 262 210 197 90 72
9
SnaBI TAC'GTA
Cuts at: 493 10671 493
Size: 10178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12880
SphI G_CATG'C
Cuts at: 1132 10238 1132
Size: 9106 3774
SspI AAT'ATT
Cuts at: 5 52 10804 5
Size: 47 10752 2081
Fragments arranged by size:
10752 2081 47
StuI AGG'CCT
Cuts at: 1517 2627 3453 4019 4338 6081 6648 7500 7608
Size: 1110 826 566 319 1743 567 852 108
Cuts at: 7608 9947 1517
Size: 2339 4450
Fragments arranged by size:
4450 2339 1743 1110 852 826 567 566
319 108
StyI C'CwwG_G
Cuts at: 513 1065 2778 3030 3333 3462 3507 4686 4734
Size: 552 1713 252 303 129 45 1179 48
Cuts at: 4734 5193 5265 6151 6946 7014 7615 7704 8025
Size: 459 72 886 795 68 601 89 321
Cuts at: 8025 9642 9699 9774 10319 513
Size: 1617 57 75 545 3074
Fragments arranged by size:
3074 1713 1617 1179 886 795 601 552
545 459 321 303 252 129 89 75
72 68 57 48 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 4682 6053 6518 6944 7501 8651
Size: 1255 612 2490 1371 465 426 557 1150
Cuts at: 8651 8921 10907 10924 11077 11262 11707 325
Size: 270 1986 17 153 185 445 1498
Fragments arranged by size:
2490 1986 1498 1371 1255 1150 612 557
465 445 426 270 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3612 4500 4797
Size: 274 80 33 51 1443 1635 888 297
Cuts at: 4797 5388 5949 6228 10077 11126 12838 96
Size: 591 561 279 3849 1049 1712 138
Fragments arranged by size:
3849 1712 1635 1443 1049 888 591 561
297 279 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 3373 3405 5781 5813 9679 9711 9806
Size: 32 1254 32 2376 32 3866 32 95
Cuts at: 9806 9838 12840 12872 2087
Size: 32 3002 32 2095
Fragments arranged by size:
3866 3002 2376 2095 1254 95 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4853 9850 1316
Size: 3537 4997 4346
Fragments arranged by size:
4997 4346 3537
XbaI T'CTAG_A
Cuts at: 10216 10216
Size: 12880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2644 5533 6679 9649 9832 10006 2644
Size: 2889 1146 2970 183 174 5518
Fragments arranged by size:
5518 2970 2889 1146 183 174
XhoI C'TCGA_G
Cuts at: 1943 2225 2315 2324 9548 1943
Size: 282 90 9 7224 5275
Fragments arranged by size:
7224 5275 282 90 9
XmaI C'CCGG_G
Cuts at: 1870 2658 5212 6381 7816 9021 9312 9330 10205
Size: 788 2554 1169 1435 1205 291 18 875
Cuts at: 10205 1870
Size: 4545
Fragments arranged by size:
4545 2554 1435 1205 1169 875 788 291
18
XmnI GAAnn'nnTTC
Cuts at: 2126 9968 11009 2126
Size: 7842 1041 3997
Fragments arranged by size:
7842 3997 1041
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6259 10688 276
Size: 53 83 186 936 4725 4429 2468
Fragments arranged by size:
4725 4429 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI
FspAI NruI NsiI PacI RsrII SacII SapI SfiI
SrfI SwaI