Restriction map for
pFN21AE2355
(Circular) (Six-base) MAPSORT of: pFN21AE2355.seq Check: 5918 from: 1 to: 7591
pFN21AE2355 7591 bp
With 182 enzymes: SgfI *
June 21, 2011 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3198 5401 278
Size: 53 83 186 936 1662 2203 2468
Fragments arranged by size:
2468 2203 1662 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4934 1937
Size: 2997 4594
Acc65I G'GTAC_C
Cuts at: 2531 4912 2531
Size: 2381 5210
AclI AA'CG_TT
Cuts at: 1563 5718 6091 1563
Size: 4155 373 3063
Fragments arranged by size:
4155 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2614 4583 4814 5654 7038 7306 1632
Size: 982 1969 231 840 1384 268 1917
Fragments arranged by size:
1969 1917 1384 982 840 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7394
AflIII A'CryG_T
Cuts at: 1203 6490 7467 1203
Size: 5287 977 1327
Fragments arranged by size:
5287 1327 977
AgeI A'CCGG_T
Cuts at: 6422 6422
Size: 7591
AhdI GACnn_n'nnGTC
Cuts at: 6320 6320
Size: 7591
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7591
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3291 3323 3291
Size: 32 7559
AlwNI CAG_nnn'CTG
Cuts at: 2624 4451 5334 6906 2624
Size: 1827 883 1572 3309
Fragments arranged by size:
3309 1827 1572 883
ApaI G_GGCC'C
Cuts at: 2275 2920 3086 3363 3530 4228 2275
Size: 645 166 277 167 698 5638
Fragments arranged by size:
5638 698 645 277 167 166
ApaLI G'TGCA_C
Cuts at: 2773 5648 6804 2773
Size: 2875 1156 3560
Fragments arranged by size:
3560 2875 1156
ApoI r'AATT_y
Cuts at: 1493 2090 4900 5126 5278 7387 1493
Size: 597 2810 226 152 2109 1697
Fragments arranged by size:
2810 2109 1697 597 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6145 160
Size: 872 5113 1606
Fragments arranged by size:
5113 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7591
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 3035 3245 3383 3407 3431 3469
Size: 73 1044 48 210 138 24 24 38
Cuts at: 3469 3863 3922 4027 4193 4262 4354 4595 4916
Size: 394 59 105 166 69 92 241 321
Cuts at: 4916 1870
Size: 4545
Fragments arranged by size:
4545 1044 394 321 241 210 166 138
105 92 73 69 59 48 38 24
24
BamHI G'GATC_C
Cuts at: 4921 5288 4921
Size: 367 7224
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 2614 2860 2893 3127
Size: 325 217 627 744 83 246 33 234
Cuts at: 3127 3274 3373 3460 3481 3643 3656 3670 3698
Size: 147 99 87 21 162 13 14 28
Cuts at: 3698 4097 4217 4591 4912 6367 618
Size: 399 120 374 321 1455 1842
Fragments arranged by size:
1842 1455 744 627 399 374 325 321
246 234 217 162 147 120 99 87
83 33 28 21 14 13
BanII G_rGCy'C
Cuts at: 1391 2275 2468 2495 2920 3027 3036 3066 3086
Size: 884 193 27 425 107 9 30 20
Cuts at: 3086 3206 3363 3384 3408 3432 3453 3476 3530
Size: 120 157 21 24 24 21 23 54
Cuts at: 3530 3579 3870 3965 4002 4228 4383 4910 1391
Size: 49 291 95 37 226 155 527 4072
Fragments arranged by size:
4072 884 527 425 291 226 193 157
155 120 107 95 54 49 37 30
27 24 24 23 21 21 20 9
BbeI G_GCGC'C
Cuts at: 2864 2897 3131 3278 3377 3464 3485 3647 3660
Size: 33 234 147 99 87 21 162 13
Cuts at: 3660 3674 4101 2864
Size: 14 427 6354
Fragments arranged by size:
6354 427 234 162 147 99 87 33
21 14 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3198 4545 928
Size: 971 1299 1347 3974
Fragments arranged by size:
3974 1347 1299 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5782 5816 1903
Size: 34 3845 34 3678
Fragments arranged by size:
3845 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5483 6693 5483
Size: 1210 6381
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7591
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3367 3665 3944
Size: 107 122 71 631 605 1695 298 279
Cuts at: 3944 6202 136
Size: 2258 1525
Fragments arranged by size:
2258 1695 1525 631 605 298 279 122
107 71
BglII A'GATC_T
Cuts at: 7586 7586
Size: 7591
BlpI GC'TnA_GC
Cuts at: 2676 4271 5008 2676
Size: 1595 737 5259
Fragments arranged by size:
5259 1595 737
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2275 2777 2920 3086 3151 3363 3530
Size: 468 17 502 143 166 65 212 167
Cuts at: 3530 4228 4555 4596 5652 6808 1790
Size: 698 327 41 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 698 502 468 327 212
167 166 143 65 41 17
BmgBI CAC'GTC
Cuts at: 1318 2521 2772 2857 1318
Size: 1203 251 85 6052
Fragments arranged by size:
6052 1203 251 85
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5322 6280 442
Size: 416 53 83 399 24 3905 958 1753
Fragments arranged by size:
3905 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3295 3016
Size: 279 7312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2406 2544 2672 3836 3844 4473 6251
Size: 1022 145 138 128 1164 8 629 1778
Cuts at: 6251 1239
Size: 2579
Fragments arranged by size:
2579 1778 1164 1022 629 145 138 128
8
Bpu10I CC'TnA_GC
Cuts at: 2759 2877 3378 2759
Size: 118 501 6972
Fragments arranged by size:
6972 501 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2986 4842 5716 6581 6879 2986
Size: 1856 874 865 298 3698
Fragments arranged by size:
3698 1856 874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2551 3863 6254 882
Size: 617 1052 1312 2391 2219
Fragments arranged by size:
2391 2219 1312 1052 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 5382 493
Size: 711 1365 2813 2702
Fragments arranged by size:
2813 2702 1365 711
BsaBI GATnn'nnATC
Cuts at: 7585 7585
Size: 7591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2861 2894 2982 3128
Size: 53 83 186 936 1328 33 88 146
Cuts at: 3128 3195 3275 3329 3374 3461 3482 3644 3657
Size: 67 80 54 45 87 21 162 13
Cuts at: 3657 3671 3762 4098 4347 5398 5780 6415 275
Size: 14 91 336 249 1051 382 635 1451
Fragments arranged by size:
1451 1328 1051 936 635 382 336 249
186 162 146 91 88 87 83 80
67 54 53 45 33 21 14 13
BsaWI w'CCGG_w
Cuts at: 2306 4062 4143 4680 6024 6422 6696 6843 7254
Size: 1756 81 537 1344 398 274 147 411
Cuts at: 7254 2306
Size: 2643
Fragments arranged by size:
2643 1756 1344 537 411 398 274 147
81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3053 3083 4169 4199 1178
Size: 30 165 30 1650 30 1086 30 4570
Fragments arranged by size:
4570 1650 1086 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2409 2641 2798 2801 2813 2938 2987
Size: 1072 151 232 157 3 12 125 49
Cuts at: 2987 3185 3365 3429 3823 3841 4193 1186
Size: 198 180 64 394 18 352 4584
Fragments arranged by size:
4584 1072 394 352 232 198 180 157
151 125 64 49 18 12 3
BseYI C'CCAG_C
Cuts at: 2260 2837 3870 4006 4229 6794 7290 2260
Size: 577 1033 136 223 2565 496 2561
Fragments arranged by size:
2565 2561 1033 577 496 223 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 1163
Size: 573 762 6256
Fragments arranged by size:
6256 762 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2714 3516 3793 3948 3978 5041
Size: 275 186 713 802 277 155 30 1063
Cuts at: 5041 5802 6830 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1063 1028 802 761 713 277 275
186 155 30
BsiHKAI G_wGCw'C
Cuts at: 2468 2777 3027 3187 3342 4170 5652 5737 6808
Size: 309 250 160 155 828 1482 85 1071
Cuts at: 6808 2468
Size: 3251
Fragments arranged by size:
3251 1482 1071 828 309 250 160 155
85
BsmI GAATG_Cn'
Cuts at: 5102 5195 5102
Size: 93 7498
BsmBI CGTCTCn'nnnn_
Cuts at: 2590 3392 3769 2590
Size: 802 377 6412
Fragments arranged by size:
6412 802 377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2275 2468 2495 2777 2920 3027
Size: 399 468 17 193 27 282 143 107
Cuts at: 3027 3036 3066 3086 3151 3187 3206 3342 3363
Size: 9 30 20 65 36 19 136 21
Cuts at: 3363 3384 3408 3432 3453 3476 3530 3579 3870
Size: 21 24 24 21 23 54 49 291
Cuts at: 3870 3965 4002 4170 4228 4383 4555 4596 4910
Size: 95 37 168 58 155 172 41 314
Cuts at: 4910 5652 5737 6808 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 314 291 282
193 172 168 155 143 136 107 95
85 65 58 54 49 41 37 36
30 27 24 24 23 21 21 21
20 19 17 9
BspEI T'CCGG_A
Cuts at: 2306 7254 2306
Size: 4948 2643
BspHI T'CATG_A
Cuts at: 5480 7210 5480
Size: 1730 5861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4946 844
Size: 4102 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3419 3679 3813 4270 4363 5478 1394
Size: 429 1596 260 134 457 93 1115 3507
Fragments arranged by size:
3507 1596 1115 457 429 260 134 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 2193 6086 6260 65
Size: 1183 945 3893 174 1396
Fragments arranged by size:
3893 1396 1183 945 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2456 2890 3370 3478 3504 3649 4021
Size: 110 646 434 480 108 26 145 372
Cuts at: 4021 4349 6235 6422 1700
Size: 328 1886 187 2869
Fragments arranged by size:
2869 1886 646 480 434 372 328 187
145 110 108 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7591
BssHII G'CGCG_C
Cuts at: 1925 2740 1925
Size: 815 6776
BssSI C'ACGA_G
Cuts at: 3736 3991 5651 6663 3736
Size: 255 1660 1012 4664
Fragments arranged by size:
4664 1660 1012 255
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7591
BstBI TT'CG_AA
Cuts at: 6395 6395
Size: 7591
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7591
BstYI r'GATC_y
Cuts at: 1972 2595 4921 5288 5673 5690 7142 7228 7240
Size: 623 2326 367 385 17 1452 86 12
Cuts at: 7240 7586 1972
Size: 346 1977
Fragments arranged by size:
2326 1977 1452 623 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3225 3915 3966 3225
Size: 690 51 6850
Fragments arranged by size:
6850 690 51
BtgI C'CryG_G
Cuts at: 513 1065 3156 3456 3510 513
Size: 552 2091 300 54 4594
Fragments arranged by size:
4594 2091 552 300 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2721 487
Size: 683 56 1495 5357
Fragments arranged by size:
5357 1495 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4770 5107 5901 5921 3310
Size: 1460 337 794 20 4980
Fragments arranged by size:
4980 1460 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7591
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7556
DraI TTT'AAA
Cuts at: 4895 4895
Size: 7591
DraIII CAC_nnn'GTG
Cuts at: 2521 2521
Size: 7591
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 4564 6598 809
Size: 507 1266 1982 2034 1802
Fragments arranged by size:
2034 1982 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2961 3012 3153
Size: 54 1425 50 275 18 1131 51 141
Cuts at: 3153 3513 3790 3936 3945 3975 5038 5927 7556
Size: 360 277 146 9 30 1063 889 1629
Cuts at: 7556 8
Size: 43
Fragments arranged by size:
1629 1425 1131 1063 889 360 277 275
146 141 54 51 50 43 30 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 3513 3790 3945 3975 5038 1537
Size: 275 1701 277 155 30 1063 4090
Fragments arranged by size:
4090 1701 1063 277 275 155 30
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2823 4118 4790 5521 1353
Size: 767 703 1295 672 731 3423
Fragments arranged by size:
3423 1295 767 731 703 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3123 3182 3701 3917 4406 6178
Size: 1064 138 1534 59 519 216 489 1772
Cuts at: 6178 6552 6698 387
Size: 374 146 1280
Fragments arranged by size:
1772 1534 1280 1064 519 489 374 216
146 138 59
EcoICRI GAG'CTC
Cuts at: 2466 3025 2466
Size: 559 7032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2406 2544 2614 2672 3836 3844
Size: 393 629 145 138 70 58 1164 8
Cuts at: 3844 4473 4583 4814 5654 6251 7038 7306 1239
Size: 629 110 231 840 597 787 268 1524
Fragments arranged by size:
1524 1164 840 787 629 629 597 393
268 231 145 138 110 70 58 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2421 2060
Size: 361 7230
EcoO109I rG'GnC_Cy
Cuts at: 2272 2525 2873 2916 2995 3082 3083 3359 3360
Size: 253 348 43 79 87 1 276 1
Cuts at: 3360 3526 3527 4224 4225 4448 4512 2272
Size: 166 1 697 1 223 64 5351
Fragments arranged by size:
5351 697 348 276 253 223 166 87
79 64 43 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4900 5278 4900
Size: 378 7213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4720 4720
Size: 7591
FspI TGC'GCA
Cuts at: 4409 6097 4409
Size: 1688 5903
HaeII r_GCGC'y
Cuts at: 1678 2836 2864 2897 3131 3278 3377 3464 3485
Size: 1158 28 33 234 147 99 87 21
Cuts at: 3485 3622 3647 3660 3674 4101 6738 1678
Size: 137 25 13 14 427 2637 2531
Fragments arranged by size:
2637 2531 1158 427 234 147 137 99
87 33 28 25 21 14 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 3023 3053 3055
Size: 32 135 32 784 32 635 30 2
Cuts at: 3055 3085 4085 4117 4811 4843 6232 6264 6306
Size: 30 1000 32 694 32 1389 32 42
Cuts at: 6306 6338 1373
Size: 32 2626
Fragments arranged by size:
2626 1389 1000 784 694 635 135 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4935 5181 669
Size: 988 281 2997 246 3079
Fragments arranged by size:
3079 2997 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2033 4860 748
Size: 1285 2827 3479
Fragments arranged by size:
3479 2827 1285
HpaI GTT'AAC
Cuts at: 5181 5181
Size: 7591
KasI G'GCGC_C
Cuts at: 2860 2893 3127 3274 3373 3460 3481 3643 3656
Size: 33 234 147 99 87 21 162 13
Cuts at: 3656 3670 4097 2860
Size: 14 427 6354
Fragments arranged by size:
6354 427 234 162 147 99 87 33
21 14 13
KpnI G_GTAC'C
Cuts at: 2535 4916 2535
Size: 2381 5210
MfeI C'AATT_G
Cuts at: 5190 5190
Size: 7591
MluI A'CGCG_T
Cuts at: 7467 7467
Size: 7591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6705 6889 1305
Size: 5400 184 2007
Fragments arranged by size:
5400 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5769
Fragments arranged by size:
5769 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 5549 5908 6067 518
Size: 648 852 3531 359 159 2042
Fragments arranged by size:
3531 2042 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2710 3512 3554 3775 3789 4405 4673 4792
Size: 1104 802 42 221 14 616 268 119
Cuts at: 4792 5007 5684 6832 7077 1606
Size: 215 677 1148 245 2120
Fragments arranged by size:
2120 1148 1104 802 677 616 268 245
221 215 119 42 14
NaeI GCC'GGC
Cuts at: 2458 3372 3480 4023 4351 2458
Size: 914 108 543 328 5698
Fragments arranged by size:
5698 914 543 328 108
NarI GG'CG_CC
Cuts at: 2861 2894 3128 3275 3374 3461 3482 3644 3657
Size: 33 234 147 99 87 21 162 13
Cuts at: 3657 3671 4098 2861
Size: 14 427 6354
Fragments arranged by size:
6354 427 234 162 147 99 87 33
21 14 13
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7039
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7591
NgoMIV G'CCGG_C
Cuts at: 2456 3370 3478 4021 4349 2456
Size: 914 108 543 328 5698
Fragments arranged by size:
5698 914 543 328 108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7591
NotI GC'GGCC_GC
Cuts at: 3513 3790 3975 5038 3513
Size: 277 185 1063 6066
Fragments arranged by size:
6066 1063 277 185
NruI TCG'CGA
Cuts at: 3200 3288 3806 3200
Size: 88 518 6985
Fragments arranged by size:
6985 518 88
NspI r_CATG'y
Cuts at: 1132 1229 4876 4949 6494 1132
Size: 97 3647 73 1545 2229
Fragments arranged by size:
3647 2229 1545 97 73
PasI CC'CwG_GG
Cuts at: 1348 3365 1348
Size: 2017 5574
PciI A'CATG_T
Cuts at: 6490 6490
Size: 7591
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7591
PfoI T'CCnGG_A
Cuts at: 3342 4829 3342
Size: 1487 6104
PmeI GTTT'AAAC
Cuts at: 4895 4895
Size: 7591
PmlI CAC'GTG
Cuts at: 2569 2569
Size: 7591
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5660 5692 7173 7205 5660
Size: 32 1481 32 6046
Fragments arranged by size:
6046 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2525 2995 4512 2525
Size: 470 1517 5604
Fragments arranged by size:
5604 1517 470
PshAI GACnn'nnGTC
Cuts at: 1613 2523 1613
Size: 910 6681
PsiI TTA'TAA
Cuts at: 4886 5161 4886
Size: 275 7316
PspOMI G'GGCC_C
Cuts at: 2271 2916 3082 3359 3526 4224 2271
Size: 645 166 277 167 698 5638
Fragments arranged by size:
5638 698 645 277 167 166
PstI C_TGCA'G
Cuts at: 830 2050 2379 2644 2674 2943 4311 4441 4765
Size: 1220 329 265 30 269 1368 130 324
Cuts at: 4765 4943 830
Size: 178 3478
Fragments arranged by size:
3478 1368 1220 329 324 269 265 178
130 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7591
PvuII CAG'CTG
Cuts at: 4792 4792
Size: 7591
SacI G_AGCT'C
Cuts at: 2468 3027 2468
Size: 559 7032
SacII CC_GC'GG
Cuts at: 3513 3513
Size: 7591
SalI G'TCGA_C
Cuts at: 1936 4933 1936
Size: 2997 4594
SapI GCTCTTCn'nnn_
Cuts at: 2823 2823
Size: 7591
SbfI CC_TGCA'GG
Cuts at: 4943 4943
Size: 7591
ScaI AGT'ACT
Cuts at: 5839 7551 5839
Size: 1712 5879
SfcI C'TryA_G
Cuts at: 826 1045 2046 2375 2640 2670 2939 4307 4437
Size: 219 1001 329 265 30 269 1368 130
Cuts at: 4437 4761 4939 6074 6755 6946 826
Size: 324 178 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 1001 681 329 324 269
265 219 191 178 130 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3367 3944 3367
Size: 577 7014
SfoI GGC'GCC
Cuts at: 2862 2895 3129 3276 3375 3462 3483 3645 3658
Size: 33 234 147 99 87 21 162 13
Cuts at: 3658 3672 4099 2862
Size: 14 427 6354
Fragments arranged by size:
6354 427 234 162 147 99 87 33
21 14 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7591
SgrAI Cr'CCGG_yG
Cuts at: 1700 2890 3478 3649 1700
Size: 1190 588 171 5642
Fragments arranged by size:
5642 1190 588 171
SmaI CCC'GGG
Cuts at: 1872 2989 4356 4918 1872
Size: 1117 1367 562 4545
Fragments arranged by size:
4545 1367 1117 562
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3001 4857 5695 6596 6858 820
Size: 197 926 1058 1856 838 901 262 1553
Fragments arranged by size:
1856 1553 1058 926 901 838 262 197
SnaBI TAC'GTA
Cuts at: 493 5382 493
Size: 4889 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7591
SphI G_CATG'C
Cuts at: 1132 4949 1132
Size: 3817 3774
SspI AAT'ATT
Cuts at: 5 52 5515 5
Size: 47 5463 2081
Fragments arranged by size:
5463 2081 47
StuI AGG'CCT
Cuts at: 1517 2055 1517
Size: 538 7053
StyI C'CwwG_G
Cuts at: 513 1065 2056 3624 4237 4515 4587 5030 513
Size: 552 991 1568 613 278 72 443 3074
Fragments arranged by size:
3074 1568 991 613 552 443 278 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3461 4543 5618 5635 5788 5973
Size: 1255 1860 21 1082 1075 17 153 185
Cuts at: 5973 6418 325
Size: 445 1498
Fragments arranged by size:
1860 1498 1255 1082 1075 445 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4814 5837 7549
Size: 274 80 33 51 1443 2837 1023 1712
Cuts at: 7549 96
Size: 138
Fragments arranged by size:
2837 1712 1443 1023 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4169 4201 7551 7583 4169
Size: 32 3350 32 4177
Fragments arranged by size:
4177 3350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7591
XbaI T'CTAG_A
Cuts at: 4927 4927
Size: 7591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 3055 2020
Size: 1035 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7591
XmaI C'CCGG_G
Cuts at: 1870 2987 4354 4916 1870
Size: 1117 1367 562 4545
Fragments arranged by size:
4545 1367 1117 562
XmnI GAAnn'nnTTC
Cuts at: 3722 5720 3722
Size: 1998 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3196 5399 276
Size: 53 83 186 936 1662 2203 2468
Fragments arranged by size:
2468 2203 1662 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I EcoRV FseI FspAI NsiI PacI PspXI
PsrI RsrII SanDI SexAI SrfI SwaI