Restriction map for
pFN21AE2352
(Circular) (Six-base) MAPSORT of: pFN21AE2352.seq Check: 4850 from: 1 to: 5638
pFN21AE2352 5638 bp
With 182 enzymes: SgfI *
June 2, 2011 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2510 2510
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2221 2981 1937
Size: 284 760 4594
Fragments arranged by size:
4594 760 284
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 3701 5085 5353 1632
Size: 532 1537 1384 268 1917
Fragments arranged by size:
1917 1537 1384 532 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2417 3381 4953 2417
Size: 964 1572 3102
Fragments arranged by size:
3102 1572 964
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2247 2947 3173 3325 5434 1493
Size: 754 700 226 152 2109 1697
Fragments arranged by size:
2109 1697 754 700 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 2963 1870
Size: 73 624 396 4545
Fragments arranged by size:
4545 624 396 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 928
Size: 971 617 4050
Fragments arranged by size:
4050 971 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2696 3699 4855 1790
Size: 906 1003 1156 2573
Fragments arranged by size:
2573 1156 1003 906
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 2712 2845
Size: 416 53 83 399 24 1169 126 133
Cuts at: 2845 3369 4327 442
Size: 524 958 1753
Fragments arranged by size:
1753 1169 958 524 416 399 133 126
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2338 2047
Size: 291 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 2774 4298 1239
Size: 1295 240 1524 2579
Fragments arranged by size:
2579 1524 1295 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 3763 4628 4926 2003
Size: 1760 865 298 2715
Fragments arranged by size:
2715 1760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2095 2338 4301 882
Size: 617 596 243 1963 2219
Fragments arranged by size:
2219 1963 617 596 243
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 1186
Size: 1018 4620
BseYI C'CCAG_C
Cuts at: 2417 2765 4841 5337 2417
Size: 348 2076 496 2718
Fragments arranged by size:
2718 2076 496 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 1163
Size: 573 567 4498
Fragments arranged by size:
4498 573 567
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 3699 3784 4855 2451
Size: 1248 85 1071 3234
Fragments arranged by size:
3234 1248 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2696 2957 3699 3784 4855 1391
Size: 399 661 245 261 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 399 261 245 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2510 2993 844
Size: 1666 483 3489
Fragments arranged by size:
3489 1666 483
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 4133 4307 65
Size: 1183 943 1942 174 1396
Fragments arranged by size:
1942 1396 1183 943 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2222 2222
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2584 2853 513
Size: 552 1519 269 3298
Fragments arranged by size:
3298 1519 552 269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 2605 2760
Size: 54 1425 50 275 18 597 178 155
Cuts at: 2760 3085 3974 5603 8
Size: 325 889 1629 43
Fragments arranged by size:
1629 1425 889 597 325 275 178 155
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2534 2774 3701 4298 5085 5353
Size: 393 532 370 240 927 597 787 268
Cuts at: 5353 1239
Size: 1524
Fragments arranged by size:
1524 927 787 597 532 393 370 268
240
EcoNI CCTnn'n_nnAGG
Cuts at: 2167 2167
Size: 5638
EcoRI G'AATT_C
Cuts at: 2247 2947 3325 2247
Size: 700 378 4560
Fragments arranged by size:
4560 700 378
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 4785 1678
Size: 3107 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2694 2722 2726 2754 4279
Size: 32 135 32 1122 28 4 28 1525
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1525 1122 135 42 32 32 32
32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2341 748
Size: 1593 4045
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 2607 10
Size: 54 1425 343 597 178 3041
Fragments arranged by size:
3041 1425 597 343 178 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 3596 3955 4114 518
Size: 648 1303 1127 359 159 2042
Fragments arranged by size:
2042 1303 1127 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 2765 2777 3054 3731 4879 5124 1606
Size: 948 211 12 277 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 277 245 211 12
NcoI C'CATG_G
Cuts at: 513 1065 2584 513
Size: 552 1519 3567
Fragments arranged by size:
3567 1519 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2417 1108
Size: 1309 4329
PfoI T'CCnGG_A
Cuts at: 2072 2072
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2616 2990 830
Size: 1786 374 3478
Fragments arranged by size:
3478 1786 374
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2777 2777
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2612 2986 4121 4802 4993 826
Size: 219 1567 374 1135 681 191 1471
Fragments arranged by size:
1567 1471 1135 681 374 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 3742 4643 4905 820
Size: 197 926 75 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197 75
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 4582
StyI C'CwwG_G
Cuts at: 513 1065 2584 3077 513
Size: 552 1519 493 3074
Fragments arranged by size:
3074 1519 552 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2223 2647 3665 3682 3835 4020 4465
Size: 1255 643 424 1018 17 153 185 445
Cuts at: 4465 325
Size: 1498
Fragments arranged by size:
1498 1255 1018 643 445 424 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2659 2691 5598 5630 2659
Size: 32 2907 32 2667
Fragments arranged by size:
2907 2667 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2825 1316
Size: 1509 4129
XbaI T'CTAG_A
Cuts at: 2809 2974 2809
Size: 165 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2652 2652
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI