Restriction map for
pFN21AE2344
(Circular) (Six-base) MAPSORT of: pFN21AE2344.seq Check: 4265 from: 1 to: 8713
pFN21AE2344 8713 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3374 5880 3374
Size: 2506 6207
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2561 6523 278
Size: 53 83 186 936 1025 3962 2468
Fragments arranged by size:
3962 2468 1025 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5469 6056 1937
Size: 3532 587 4594
Fragments arranged by size:
4594 3532 587
Acc65I G'GTAC_C
Cuts at: 6034 6034
Size: 8713
AclI AA'CG_TT
Cuts at: 1563 6840 7213 1563
Size: 5277 373 3063
Fragments arranged by size:
5277 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 3490 3500 4148 6776 8160 8428 1632
Size: 872 986 10 648 2628 1384 268 1917
Fragments arranged by size:
2628 1917 1384 986 872 648 268 10
AfeI AGC'GCT
Cuts at: 2664 3449 3641 4236 2664
Size: 785 192 595 7141
Fragments arranged by size:
7141 785 595 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8516
AflIII A'CryG_T
Cuts at: 1203 7612 8589 1203
Size: 6409 977 1327
Fragments arranged by size:
6409 1327 977
AgeI A'CCGG_T
Cuts at: 7544 7544
Size: 8713
AhdI GACnn_n'nnGTC
Cuts at: 2323 2401 2557 7442 2323
Size: 78 156 4885 3594
Fragments arranged by size:
4885 3594 156 78
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2318 4397 1184
Size: 1134 2079 5500
Fragments arranged by size:
5500 2079 1134
AlwNI CAG_nnn'CTG
Cuts at: 3004 4435 4692 4747 6456 8028 3004
Size: 1431 257 55 1709 1572 3689
Fragments arranged by size:
3689 1709 1572 1431 257 55
ApaI G_GGCC'C
Cuts at: 2042 2127 2184 2216 2835 2866 3919 4641 4791
Size: 85 57 32 619 31 1053 722 150
Cuts at: 4791 5658 5677 5874 2042
Size: 867 19 197 4881
Fragments arranged by size:
4881 1053 867 722 619 197 150 85
57 32 31 19
ApaLI G'TGCA_C
Cuts at: 2339 2435 6770 7926 2339
Size: 96 4335 1156 3126
Fragments arranged by size:
4335 3126 1156 96
ApoI r'AATT_y
Cuts at: 1493 6022 6248 6400 8509 1493
Size: 4529 226 152 2109 1697
Fragments arranged by size:
4529 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 5488 5767 5488
Size: 279 8434
AseI AT'TA_AT
Cuts at: 160 1032 7267 160
Size: 872 6235 1606
Fragments arranged by size:
6235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2176 2242 2751 3737 3954 4784
Size: 73 91 142 66 509 986 217 830
Cuts at: 4784 5201 5327 5438 5477 5819 5999 6038 1870
Size: 417 126 111 39 342 180 39 4545
Fragments arranged by size:
4545 986 830 509 417 342 217 180
142 126 111 91 73 66 39 39
AvrII C'CTAG_G
Cuts at: 4921 4921
Size: 8713
BamHI G'GATC_C
Cuts at: 6043 6410 6043
Size: 367 8346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2098 2444 2894 2993
Size: 325 217 627 225 86 346 450 99
Cuts at: 2993 3362 3937 4617 4833 4951 4971 5069 5173
Size: 369 575 680 216 118 20 98 104
Cuts at: 5173 5626 5685 5760 5966 6034 7489 618
Size: 453 59 75 206 68 1455 1842
Fragments arranged by size:
1842 1455 680 627 575 453 450 369
346 325 225 217 216 206 118 104
99 98 86 75 68 59 20
BanII G_rGCy'C
Cuts at: 1391 2042 2127 2184 2216 2480 2501 2769 2835
Size: 651 85 57 32 264 21 268 66
Cuts at: 2835 2858 2866 2877 3036 3744 3919 4083 4394
Size: 23 8 11 159 708 175 164 311
Cuts at: 4394 4641 4791 5061 5168 5272 5397 5658 5677
Size: 247 150 270 107 104 125 261 19
Cuts at: 5677 5874 5981 6032 1391
Size: 197 107 51 4072
Fragments arranged by size:
4072 708 651 311 270 268 264 261
247 197 175 164 159 150 125 107
107 104 85 66 57 51 32 23
21 19 11 8
BbeI G_GCGC'C
Cuts at: 2016 2102 2448 2898 4621 4837 4975 5073 5177
Size: 86 346 450 1723 216 138 98 104
Cuts at: 5177 5630 2016
Size: 453 5099
Fragments arranged by size:
5099 1723 453 450 346 216 138 104
98 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2364 4345 4540 4582 928
Size: 971 445 20 1981 195 42 5059
Fragments arranged by size:
5059 1981 971 445 195 42 20
BbvCI CC'TCA_GC
Cuts at: 2784 5828 2784
Size: 3044 5669
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2910 2944 4336 4370 6904
Size: 34 50 34 889 34 1392 34 2534
Cuts at: 6904 6938 1903
Size: 34 3678
Fragments arranged by size:
3678 2534 1392 889 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4060 6605 7815 4060
Size: 2545 1210 4958
Fragments arranged by size:
4958 2545 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2037 2201
Size: 107 122 71 631 605 356 9 164
Cuts at: 2201 2225 2911 2984 4034 5114 5370 5613 7324
Size: 24 686 73 1050 1080 256 243 1711
Cuts at: 7324 136
Size: 1525
Fragments arranged by size:
1711 1525 1080 1050 686 631 605 356
256 243 164 122 107 73 71 24
9
BglII A'GATC_T
Cuts at: 8708 8708
Size: 8713
BlpI GC'TnA_GC
Cuts at: 4995 6130 4995
Size: 1135 7578
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2127 2184 2216 2343 2439 2587 2707
Size: 252 85 57 32 127 96 148 120
Cuts at: 2707 2835 2866 2998 3919 3942 4146 4641 4791
Size: 128 31 132 921 23 204 495 150
Cuts at: 4791 4806 5249 5315 5658 5677 5690 5840 5874
Size: 15 443 66 343 19 13 150 34
Cuts at: 5874 5969 6774 7930 1790
Size: 95 805 1156 2573
Fragments arranged by size:
2573 1156 921 805 495 443 343 252
204 150 150 148 132 128 127 120
96 95 85 66 57 34 32 31
23 19 15 13
BmgBI CAC'GTC
Cuts at: 1318 2254 3195 1318
Size: 936 941 6836
Fragments arranged by size:
6836 941 936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5920 6444 7402
Size: 416 53 83 399 24 4503 524 958
Cuts at: 7402 442
Size: 1753
Fragments arranged by size:
4503 1753 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 3658 4834 2494
Size: 1164 1176 6373
Fragments arranged by size:
6373 1176 1164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 2865 3335 4481 4657 5411 5582 7373
Size: 1322 304 470 1146 176 754 171 1791
Cuts at: 7373 1239
Size: 2579
Fragments arranged by size:
2579 1791 1322 1146 754 470 304 176
171
Bpu10I CC'TnA_GC
Cuts at: 2784 3030 5828 2784
Size: 246 2798 5669
Fragments arranged by size:
5669 2798 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3221 4232 6838 7703 8001 3221
Size: 1011 2606 865 298 3933
Fragments arranged by size:
3933 2606 1011 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3671 7376 882
Size: 617 2172 3705 2219
Fragments arranged by size:
3705 2219 2172 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 4063 6504 493
Size: 711 1904 955 2441 2702
Fragments arranged by size:
2702 2441 1904 955 711
BsaBI GATnn'nnATC
Cuts at: 8707 8707
Size: 8713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2099 2445 2558
Size: 53 83 186 936 480 86 346 113
Cuts at: 2558 2895 3561 4266 4545 4618 4834 4972 5070
Size: 337 666 705 279 73 216 138 98
Cuts at: 5070 5174 5627 5668 6520 6902 7537 275
Size: 104 453 41 852 382 635 1451
Fragments arranged by size:
1451 936 852 705 666 635 480 453
382 346 337 279 216 186 138 113
104 98 86 83 73 53 41
BsaWI w'CCGG_w
Cuts at: 3424 5940 7146 7544 7818 7965 8376 3424
Size: 2516 1206 398 274 147 411 3761
Fragments arranged by size:
3761 2516 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4376 4406 4454 4484 5384
Size: 30 165 30 2973 30 48 30 900
Cuts at: 5384 5414 1178
Size: 30 4477
Fragments arranged by size:
4477 2973 900 165 48 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2558 2771 2801 3650 4070 5280 1186
Size: 922 450 213 30 849 420 1210 4619
Fragments arranged by size:
4619 1210 922 849 450 420 213 30
BseYI C'CCAG_C
Cuts at: 2494 2646 2706 2835 3213 4751 5218 5380 5499
Size: 152 60 129 378 1538 467 162 119
Cuts at: 5499 5774 5781 7916 8412 2494
Size: 275 7 2135 496 2795
Fragments arranged by size:
2795 2135 1538 496 467 378 275 162
152 129 119 60 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4367 1163
Size: 573 2631 5509
Fragments arranged by size:
5509 2631 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2153 2196 2220 4778 4992 5277
Size: 275 186 152 43 24 2558 214 285
Cuts at: 5277 5455 5469 5532 5696 6163 6924 7952 1540
Size: 178 14 63 164 467 761 1028 2301
Fragments arranged by size:
2558 2301 1028 761 467 285 275 214
186 178 164 152 63 43 24 14
BsiHKAI G_wGCw'C
Cuts at: 2277 2343 2439 2460 2655 2700 3036 4293 4605
Size: 66 96 21 195 45 336 1257 312
Cuts at: 4605 6774 6859 7930 2277
Size: 2169 85 1071 3060
Fragments arranged by size:
3060 2169 1257 1071 336 312 195 96
85 66 45 21
BsiWI C'GTAC_G
Cuts at: 2187 5035 2187
Size: 2848 5865
BsmI GAATG_Cn'
Cuts at: 6224 6317 6224
Size: 93 8620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2127 2184 2216 2277 2343 2439
Size: 399 252 85 57 32 61 66 96
Cuts at: 2439 2460 2480 2501 2587 2655 2700 2707 2769
Size: 21 20 21 86 68 45 7 62
Cuts at: 2769 2835 2858 2866 2877 2998 3036 3744 3919
Size: 66 23 8 11 121 38 708 175
Cuts at: 3919 3942 4083 4146 4293 4394 4605 4641 4791
Size: 23 141 63 147 101 211 36 150
Cuts at: 4791 4806 5061 5168 5249 5272 5315 5397 5658
Size: 15 255 107 81 23 43 82 261
Cuts at: 5658 5677 5690 5840 5874 5969 5981 6032 6774
Size: 19 13 150 34 95 12 51 742
Cuts at: 6774 6859 7930 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 708 399 261 255 252
211 175 150 150 147 141 121 107
101 96 95 86 85 85 82 81
68 66 66 63 62 61 57 51
45 43 38 36 34 32 23 23
23 21 21 20 19 15 13 12
11 8 7
BspEI T'CCGG_A
Cuts at: 8376 8376
Size: 8713
BspHI T'CATG_A
Cuts at: 3096 4044 6602 8332 3096
Size: 948 2558 1730 3477
Fragments arranged by size:
3477 2558 1730 948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 3374 5517 5880 6068 844
Size: 1484 1046 2143 363 188 3489
Fragments arranged by size:
3489 2143 1484 1046 363 188
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 4465 4994 5279 6600 1394
Size: 429 332 2310 529 285 1321 3507
Fragments arranged by size:
3507 2310 1321 529 429 332 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 3715 7208 7382 65
Size: 1183 2467 3493 174 1396
Fragments arranged by size:
3493 2467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2117 3680 4777 4987 5066 5170
Size: 110 205 102 1563 1097 210 79 104
Cuts at: 5170 5763 7357 7544 1700
Size: 593 1594 187 2869
Fragments arranged by size:
2869 1594 1563 1097 593 210 205 187
110 104 102 79
BsrGI T'GTAC_A
Cuts at: 96 4683 96
Size: 4587 4126
BssHII G'CGCG_C
Cuts at: 1925 2368 2370 2724 3598 3701 4813 4943 4945
Size: 443 2 354 874 103 1112 130 2
Cuts at: 4945 5488 5767 5855 1925
Size: 543 279 88 4783
Fragments arranged by size:
4783 1112 874 543 443 354 279 130
103 88 2 2
BssSI C'ACGA_G
Cuts at: 2168 2300 2453 2580 5833 6773 7785 2168
Size: 132 153 127 3253 940 1012 3096
Fragments arranged by size:
3253 3096 1012 940 153 132 127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8713
BstBI TT'CG_AA
Cuts at: 2355 7517 2355
Size: 5162 3551
BstEII G'GTnAC_C
Cuts at: 1184 3526 1184
Size: 2342 6371
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 4417 2843
Size: 1574 7139
BstYI r'GATC_y
Cuts at: 1972 2730 2804 4482 6043 6410 6795 6812 8264
Size: 758 74 1678 1561 367 385 17 1452
Cuts at: 8264 8350 8362 8708 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1678 1561 1452 758 385 367 346
86 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2026 2220 2262 2656 3070 3855 3942
Size: 552 961 194 42 394 414 785 87
Cuts at: 3942 4155 4569 4772 4955 5112 5690 5711 513
Size: 213 414 203 183 157 578 21 3515
Fragments arranged by size:
3515 961 785 578 552 414 414 394
213 203 194 183 157 87 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4360 487
Size: 683 56 3134 4840
Fragments arranged by size:
4840 3134 683 56
BtsI GCAGTG_nn'
Cuts at: 2722 2928 4293 4627 5548 6229 7023 7043 2722
Size: 206 1365 334 921 681 794 20 4392
Fragments arranged by size:
4392 1365 921 794 681 334 206 20
ClaI AT'CG_AT
Cuts at: 1554 3555 1554
Size: 2001 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8678
DraI TTT'AAA
Cuts at: 6017 6017
Size: 8713
DraIII CAC_nnn'GTG
Cuts at: 2347 4609 2347
Size: 2262 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3662 7720 809
Size: 507 2346 4058 1802
Fragments arranged by size:
4058 2346 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2150 2193 2217
Size: 54 1425 50 275 18 320 43 24
Cuts at: 2217 2430 2590 2976 2985 3037 3306 3609 3852
Size: 213 160 386 9 52 269 303 243
Cuts at: 3852 4035 4566 4775 4875 4989 5115 5186 5274
Size: 183 531 209 100 114 126 71 88
Cuts at: 5274 5452 5529 6160 7049 8678 8
Size: 178 77 631 889 1629 43
Fragments arranged by size:
1629 1425 889 631 531 386 320 303
275 269 243 213 209 183 178 160
126 114 100 88 77 71 54 52
50 43 43 24 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2150 2193 2217 4775 4989 5274 5452
Size: 275 338 43 24 2558 214 285 178
Cuts at: 5452 5529 6160 1537
Size: 77 631 4090
Fragments arranged by size:
4090 2558 631 338 285 275 214 178
77 43 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2506 2747 2886 6643 1353
Size: 1153 241 139 3757 3423
Fragments arranged by size:
3757 3423 1153 241 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 3221 5271 5277 7300 7674
Size: 1064 138 1134 498 2050 6 2023 374
Cuts at: 7674 7820 387
Size: 146 1280
Fragments arranged by size:
2050 2023 1280 1134 1064 498 374 146
138 6
EcoICRI GAG'CTC
Cuts at: 3034 3034
Size: 8713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 2561 2865 3335 3490 3500 4148
Size: 393 872 57 304 470 155 10 648
Cuts at: 4148 4481 4657 5411 5582 6776 7373 8160 8428
Size: 333 176 754 171 1194 597 787 268
Cuts at: 8428 1239
Size: 1524
Fragments arranged by size:
1524 1194 872 787 754 648 597 470
393 333 304 268 176 171 155 57
10
EcoNI CCTnn'n_nnAGG
Cuts at: 4385 4672 4385
Size: 287 8426
EcoO109I rG'GnC_Cy
Cuts at: 2038 2039 2180 2831 2832 2862 2863 3001 3916
Size: 1 141 651 1 30 1 138 915
Cuts at: 3916 4104 4637 4918 5655 5785 5824 5871 5959
Size: 188 533 281 737 130 39 47 88
Cuts at: 5959 2038
Size: 4792
Fragments arranged by size:
4792 915 737 651 533 281 188 141
138 130 88 47 39 30 1 1
1
EcoRI G'AATT_C
Cuts at: 6022 6400 6022
Size: 378 8335
FspI TGC'GCA
Cuts at: 2237 7219 2237
Size: 4982 3731
HaeII r_GCGC'y
Cuts at: 1678 2016 2069 2102 2168 2270 2448 2666 2898
Size: 338 53 33 66 102 178 218 232
Cuts at: 2898 3451 3643 3868 4238 4621 4701 4837 4975
Size: 553 192 225 370 383 80 136 138
Cuts at: 4975 5073 5177 5437 5630 7860 1678
Size: 98 104 260 193 2230 2531
Fragments arranged by size:
2531 2230 553 383 370 338 260 232
225 218 193 192 178 138 136 104
102 98 80 66 53 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2309 2341 2387 2419 2543
Size: 32 135 32 737 32 46 32 124
Cuts at: 2543 2575 3366 3398 4058 4090 5816 5848 7354
Size: 32 791 32 660 32 1726 32 1506
Cuts at: 7354 7386 7428 7460 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1726 1506 791 737 660 135 124
46 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5470 6057 6303 669
Size: 988 281 3532 587 246 3079
Fragments arranged by size:
3532 3079 988 587 281 246
HindIII A'AGCT_T
Cuts at: 748 4325 748
Size: 3577 5136
HpaI GTT'AAC
Cuts at: 6303 6303
Size: 8713
KasI G'GCGC_C
Cuts at: 2012 2098 2444 2894 4617 4833 4971 5069 5173
Size: 86 346 450 1723 216 138 98 104
Cuts at: 5173 5626 2012
Size: 453 5099
Fragments arranged by size:
5099 1723 453 450 346 216 138 104
98 86
KpnI G_GTAC'C
Cuts at: 6038 6038
Size: 8713
MfeI C'AATT_G
Cuts at: 6312 6312
Size: 8713
MluI A'CGCG_T
Cuts at: 8589 8589
Size: 8713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 7827 8011 1305
Size: 4017 2505 184 2007
Fragments arranged by size:
4017 2505 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 2987 3308 3611 3854
Size: 54 1425 343 760 395 321 303 243
Cuts at: 3854 4568 10
Size: 714 4155
Fragments arranged by size:
4155 1425 760 714 395 343 321 303
243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 6671 7030 7189 518
Size: 648 1452 4053 359 159 2042
Fragments arranged by size:
4053 2042 1452 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2494 2658 2783 2839 2969 3072 3213
Size: 422 466 164 125 56 130 103 141
Cuts at: 3213 3247 3360 4398 4689 4774 4830 4957 4978
Size: 34 113 1038 291 85 56 127 21
Cuts at: 4978 5114 5523 5592 5679 5692 5713 5774 5923
Size: 136 409 69 87 13 21 61 149
Cuts at: 5923 6129 6806 7954 8199 1606
Size: 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 466 422 409 291
245 206 164 149 141 136 130 127
125 113 103 87 85 69 61 56
56 34 21 21 13
NaeI GCC'GGC
Cuts at: 4779 4989 5068 5172 4779
Size: 210 79 104 8320
Fragments arranged by size:
8320 210 104 79
NarI GG'CG_CC
Cuts at: 2013 2099 2445 2895 4618 4834 4972 5070 5174
Size: 86 346 450 1723 216 138 98 104
Cuts at: 5174 5627 2013
Size: 453 5099
Fragments arranged by size:
5099 1723 453 450 346 216 138 104
98 86
NcoI C'CATG_G
Cuts at: 513 1065 4569 513
Size: 552 3504 4657
Fragments arranged by size:
4657 3504 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8713
NgoMIV G'CCGG_C
Cuts at: 4777 4987 5066 5170 4777
Size: 210 79 104 8320
Fragments arranged by size:
8320 210 104 79
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8713
NotI GC'GGCC_GC
Cuts at: 2150 6160 2150
Size: 4010 4703
NspI r_CATG'y
Cuts at: 1132 1229 4192 4558 4684 6071 7616 1132
Size: 97 2963 366 126 1387 1545 2229
Fragments arranged by size:
2963 2229 1545 1387 366 126 97
PasI CC'CwG_GG
Cuts at: 1348 2678 5643 1348
Size: 1330 2965 4418
Fragments arranged by size:
4418 2965 1330
PciI A'CATG_T
Cuts at: 7612 7612
Size: 8713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4651 1108
Size: 3543 5170
PfoI T'CCnGG_A
Cuts at: 2088 4050 2088
Size: 1962 6751
PmeI GTTT'AAAC
Cuts at: 6017 6017
Size: 8713
PmlI CAC'GTG
Cuts at: 3108 3108
Size: 8713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 6782 6814 8295 8327 2756
Size: 32 3994 32 1481 32 3142
Fragments arranged by size:
3994 3142 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5785 5824 5959 5785
Size: 39 135 8539
Fragments arranged by size:
8539 135 39
PshAI GACnn'nnGTC
Cuts at: 1613 2634 1613
Size: 1021 7692
PsiI TTA'TAA
Cuts at: 6283 6283
Size: 8713
PspOMI G'GGCC_C
Cuts at: 2038 2123 2180 2212 2831 2862 3915 4637 4787
Size: 85 57 32 619 31 1053 722 150
Cuts at: 4787 5654 5673 5870 2038
Size: 867 19 197 4881
Fragments arranged by size:
4881 1053 867 722 619 197 150 85
57 32 31 19
PspXI vC'TCGA_Gb
Cuts at: 3737 5819 5999 3737
Size: 2082 180 6451
Fragments arranged by size:
6451 2082 180
PstI C_TGCA'G
Cuts at: 830 2310 2493 3276 3349 4288 4431 4697 6065
Size: 1480 183 783 73 939 143 266 1368
Cuts at: 6065 830
Size: 3478
Fragments arranged by size:
3478 1480 1368 939 783 266 183 143
73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8713
PvuII CAG'CTG
Cuts at: 2494 2783 2839 2969 3213 4689 5592 2494
Size: 289 56 130 244 1476 903 5615
Fragments arranged by size:
5615 1476 903 289 244 130 56
RsrII CG'GwC_CG
Cuts at: 5525 5525
Size: 8713
SacI G_AGCT'C
Cuts at: 3036 3036
Size: 8713
SacII CC_GC'GG
Cuts at: 2029 2659 3073 4775 4958 5115 5693 5714 2029
Size: 630 414 1702 183 157 578 21 5028
Fragments arranged by size:
5028 1702 630 578 414 183 157 21
SalI G'TCGA_C
Cuts at: 1936 5468 6055 1936
Size: 3532 587 4594
Fragments arranged by size:
4594 3532 587
SapI GCTCTTCn'nnn_
Cuts at: 2506 2747 2506
Size: 241 8472
SbfI CC_TGCA'GG
Cuts at: 6065 6065
Size: 8713
ScaI AGT'ACT
Cuts at: 6961 8673 6961
Size: 1712 7001
SexAI A'CCwGG_T
Cuts at: 3522 4605 3522
Size: 1083 7630
SfcI C'TryA_G
Cuts at: 826 1045 2306 2489 3272 3345 3745 4284 4427
Size: 219 1261 183 783 73 400 539 143
Cuts at: 4427 4693 6061 7196 7877 8068 826
Size: 266 1368 1135 681 191 1471
Fragments arranged by size:
1471 1368 1261 1135 783 681 539 400
266 219 191 183 143 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 2028 2037 2984 2028
Size: 9 947 7757
Fragments arranged by size:
7757 947 9
SfoI GGC'GCC
Cuts at: 2014 2100 2446 2896 4619 4835 4973 5071 5175
Size: 86 346 450 1723 216 138 98 104
Cuts at: 5175 5628 2014
Size: 453 5099
Fragments arranged by size:
5099 1723 453 450 346 216 138 104
98 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
SgrAI Cr'CCGG_yG
Cuts at: 1700 2015 5066 5170 5763 1700
Size: 315 3051 104 593 4650
Fragments arranged by size:
4650 3051 593 315 104
SmaI CCC'GGG
Cuts at: 1872 2244 3956 4786 6040 1872
Size: 372 1712 830 1254 4545
Fragments arranged by size:
4545 1712 1254 830 372
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 3737 4247 5327 5819 5999
Size: 197 926 1293 501 510 1080 492 180
Cuts at: 5999 6817 7718 7980 820
Size: 818 901 262 1553
Fragments arranged by size:
1553 1293 1080 926 901 818 510 501
492 262 197 180
SnaBI TAC'GTA
Cuts at: 493 6504 493
Size: 6011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8713
SphI G_CATG'C
Cuts at: 1132 6071 1132
Size: 4939 3774
SrfI GCCC'GGGC
Cuts at: 2244 2244
Size: 8713
SspI AAT'ATT
Cuts at: 5 52 6637 5
Size: 47 6585 2081
Fragments arranged by size:
6585 2081 47
StuI AGG'CCT
Cuts at: 1517 4926 1517
Size: 3409 5304
StyI C'CwwG_G
Cuts at: 513 1065 2466 4569 4842 4921 6152 513
Size: 552 1401 2103 273 79 1231 3074
Fragments arranged by size:
3074 2103 1401 1231 552 273 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3080 3751 4658 5455 6740 6757 6910
Size: 1255 1500 671 907 797 1285 17 153
Cuts at: 6910 7095 7540 325
Size: 185 445 1498
Fragments arranged by size:
1500 1498 1285 1255 907 797 671 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4611 4683 6959
Size: 274 80 33 51 1443 2634 72 2276
Cuts at: 6959 8671 96
Size: 1712 138
Fragments arranged by size:
2634 2276 1712 1443 274 138 80 72
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4376 4408 4454 4486 5384 5416 8673 8705 4376
Size: 32 46 32 898 32 3257 32 4384
Fragments arranged by size:
4384 3257 898 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3380 1316
Size: 2064 6649
XbaI T'CTAG_A
Cuts at: 6049 6049
Size: 8713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3973 4648 3973
Size: 675 8038
XhoI C'TCGA_G
Cuts at: 1943 3737 5327 5819 5999 1943
Size: 1794 1590 492 180 4657
Fragments arranged by size:
4657 1794 1590 492 180
XmaI C'CCGG_G
Cuts at: 1870 2242 3954 4784 6038 1870
Size: 372 1712 830 1254 4545
Fragments arranged by size:
4545 1712 1254 830 372
XmnI GAAnn'nnTTC
Cuts at: 6842 6842
Size: 8713
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2559 6521 276
Size: 53 83 186 936 1025 3962 2468
Fragments arranged by size:
3962 2468 1025 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI BaeI BfrBI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI NruI NsiI PacI PsrI SanDI SwaI