Restriction map for
pFN21AE2335
(Circular) (Six-base) MAPSORT of: pFN21AE2335.seq Check: 9303 from: 1 to: 9145
pFN21AE2335 9145 bp
With 182 enzymes: SgfI *
May 11, 2017 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4901 4901
Size: 9145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6955 278
Size: 53 83 186 936 5419 2468
Fragments arranged by size:
5419 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3690 4807 6488 1937
Size: 1753 1117 1681 4594
Fragments arranged by size:
4594 1753 1681 1117
Acc65I G'GTAC_C
Cuts at: 6466 6466
Size: 9145
AclI AA'CG_TT
Cuts at: 1563 7272 7645 1563
Size: 5709 373 3063
Fragments arranged by size:
5709 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3277 3420 4271 4783 5160 5814 7208 8592
Size: 1645 143 851 512 377 654 1394 1384
Cuts at: 8592 8860 1632
Size: 268 1917
Fragments arranged by size:
1917 1645 1394 1384 851 654 512 377
268 143
AflII C'TTAA_G
Cuts at: 820 1017 2866 820
Size: 197 1849 7099
Fragments arranged by size:
7099 1849 197
AflIII A'CryG_T
Cuts at: 1203 4899 5593 8044 9021 1203
Size: 3696 694 2451 977 1327
Fragments arranged by size:
3696 2451 1327 977 694
AgeI A'CCGG_T
Cuts at: 7976 7976
Size: 9145
AhdI GACnn_n'nnGTC
Cuts at: 7874 7874
Size: 9145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3056 1184
Size: 1872 7273
AlwNI CAG_nnn'CTG
Cuts at: 2098 4229 6292 6888 8460 2098
Size: 2131 2063 596 1572 2783
Fragments arranged by size:
2783 2131 2063 1572 596
ApaLI G'TGCA_C
Cuts at: 7202 8358 7202
Size: 1156 7989
ApoI r'AATT_y
Cuts at: 1493 2573 3465 3669 3979 4099 4133 4842 5251
Size: 1080 892 204 310 120 34 709 409
Cuts at: 5251 5587 6454 6680 6832 8941 1493
Size: 336 867 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 892 867 709 409 336
310 226 204 152 120 34
AseI AT'TA_AT
Cuts at: 160 1032 2214 3336 3854 5000 7699 160
Size: 872 1182 1122 518 1146 2699 1606
Fragments arranged by size:
2699 1606 1182 1146 1122 872 518
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9145
AvaI C'yCGr_G
Cuts at: 1870 1943 6470 1870
Size: 73 4527 4545
Fragments arranged by size:
4545 4527 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3306 3339 3306
Size: 33 9112
BamHI G'GATC_C
Cuts at: 3377 5917 6133 6475 6842 3377
Size: 2540 216 342 367 5680
Fragments arranged by size:
5680 2540 367 342 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3503 6466 7921 618
Size: 325 217 627 1716 2963 1455 1842
Fragments arranged by size:
2963 1842 1716 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 6464 1391
Size: 5073 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 4491 928
Size: 971 599 1993 5582
Fragments arranged by size:
5582 1993 971 599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6085 6119 7336 7370 1903
Size: 34 50 34 4064 34 1217 34 3678
Fragments arranged by size:
4064 3678 1217 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2078 3725 7037 8247 2078
Size: 1647 3312 1210 2976
Fragments arranged by size:
3312 2976 1647 1210
BclI T'GATC_A
Cuts at: 1548 4266 5938 1548
Size: 2718 1672 4755
Fragments arranged by size:
4755 2718 1672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4400 7756 136
Size: 107 122 71 631 605 2728 3356 1525
Fragments arranged by size:
3356 2728 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5644 5978 6077 6099 9140 5644
Size: 334 99 22 3041 5649
Fragments arranged by size:
5649 3041 334 99 22
BlpI GC'TnA_GC
Cuts at: 2926 6562 2926
Size: 3636 5509
Bme1580I G_kGCm'C
Cuts at: 1790 7206 8362 1790
Size: 5416 1156 2573
Fragments arranged by size:
5416 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 6114 6876
Size: 416 53 83 399 24 901 3796 762
Cuts at: 6876 7834 442
Size: 958 1753
Fragments arranged by size:
3796 1753 958 901 762 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2034 2371 3610 2034
Size: 337 1239 7569
Fragments arranged by size:
7569 1239 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2260 3405 3449 4095 5434 7805 1239
Size: 1021 1145 44 646 1339 2371 2579
Fragments arranged by size:
2579 2371 1339 1145 1021 646 44
Bpu10I CC'TnA_GC
Cuts at: 6041 6041
Size: 9145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4428 6352 7270 8135 8433 4428
Size: 1924 918 865 298 5140
Fragments arranged by size:
5140 1924 918 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 5978 7808 882
Size: 617 2490 1989 1830 2219
Fragments arranged by size:
2490 2219 1989 1830 617
BsaAI yAC'GTr
Cuts at: 493 1204 4762 5596 6936 493
Size: 711 3558 834 1340 2702
Fragments arranged by size:
3558 2702 1340 834 711
BsaBI GATnn'nnATC
Cuts at: 9139 9139
Size: 9145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2666 6952 7334 7969
Size: 53 83 186 936 1133 4286 382 635
Cuts at: 7969 275
Size: 1451
Fragments arranged by size:
4286 1451 1133 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3003 6360 7578 7976 8250 8397 8808 3003
Size: 3357 1218 398 274 147 411 3340
Fragments arranged by size:
3357 3340 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8920
Fragments arranged by size:
8920 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 3224 4656 6319 6425 1186
Size: 1438 600 1432 1663 106 3906
Fragments arranged by size:
3906 1663 1438 1432 600 106
BseYI C'CCAG_C
Cuts at: 2422 2717 2759 2832 8348 8844 2422
Size: 295 42 73 5516 496 2723
Fragments arranged by size:
5516 2723 496 295 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3987 1163
Size: 573 2251 6321
Fragments arranged by size:
6321 2251 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6595 7356 8384 1540
Size: 275 186 4594 761 1028 2301
Fragments arranged by size:
4594 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6127 7206 7291 8362 6127
Size: 1079 85 1071 6910
Fragments arranged by size:
6910 1079 1071 85
BsmI GAATG_Cn'
Cuts at: 2457 6236 6656 6749 2457
Size: 3779 420 93 4853
Fragments arranged by size:
4853 3779 420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 6127 6464 7206 7291 8362 1391
Size: 399 4337 337 742 85 1071 2174
Fragments arranged by size:
4337 2174 1071 742 399 337 85
BspEI T'CCGG_A
Cuts at: 3003 8808 3003
Size: 5805 3340
BspHI T'CATG_A
Cuts at: 5421 7034 8764 5421
Size: 1613 1730 5802
Fragments arranged by size:
5802 1730 1613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4901 6500 844
Size: 4057 1599 3489
Fragments arranged by size:
4057 3489 1599
BsrBI CCG'CTC
Cuts at: 1394 1823 7032 1394
Size: 429 5209 3507
Fragments arranged by size:
5209 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2850 5337 5429 6228 7640 7814 65
Size: 1183 1602 2487 92 799 1412 174 1396
Fragments arranged by size:
2487 1602 1412 1396 1183 799 174 92
BsrFI r'CCGG_y
Cuts at: 1700 1810 7789 7976 1700
Size: 110 5979 187 2869
Fragments arranged by size:
5979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3076 96
Size: 2980 6165
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9145
BssSI C'ACGA_G
Cuts at: 7205 8217 7205
Size: 1012 8133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9145
BstBI TT'CG_AA
Cuts at: 7949 7949
Size: 9145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9145
BstXI CCAn_nnnn'nTGG
Cuts at: 5813 5813
Size: 9145
BstYI r'GATC_y
Cuts at: 1972 3377 5644 5917 5978 6077 6099 6133 6475
Size: 1405 2267 273 61 99 22 34 342
Cuts at: 6475 6842 7227 7244 8696 8782 8794 9140 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2267 1977 1452 1405 385 367 346 342
273 99 86 61 34 22 17 12
BstZ17I GTA'TAC
Cuts at: 3691 3691
Size: 9145
Bsu36I CC'TnA_GG
Cuts at: 4398 5620 4398
Size: 1222 7923
BtgI C'CryG_G
Cuts at: 513 1065 6046 513
Size: 552 4981 3612
Fragments arranged by size:
4981 3612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2242 487
Size: 683 56 1016 7390
Fragments arranged by size:
7390 1016 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 4536 4722 5288 5441 6661 7455 7475 2408
Size: 2128 186 566 153 1220 794 20 4078
Fragments arranged by size:
4078 2128 1220 794 566 186 153 20
ClaI AT'CG_AT
Cuts at: 1554 5811 1554
Size: 4257 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9110
DraI TTT'AAA
Cuts at: 2499 3834 4066 6449 2499
Size: 1335 232 2383 5195
Fragments arranged by size:
5195 2383 1335 232
DraIII CAC_nnn'GTG
Cuts at: 3650 5201 3650
Size: 1551 7594
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8152 809
Size: 507 6836 1802
Fragments arranged by size:
6836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5557 5713 6049
Size: 54 1425 50 275 18 3727 156 336
Cuts at: 6049 6592 7481 9110 8
Size: 543 889 1629 43
Fragments arranged by size:
3727 1629 1425 889 543 336 275 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6592 1537
Size: 275 4780 4090
Fragments arranged by size:
4780 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3293 3579 4173 4674 5380 5747 6249 6317
Size: 1940 286 594 501 706 367 502 68
Cuts at: 6317 6417 7075 1353
Size: 100 658 3423
Fragments arranged by size:
3423 1940 706 658 594 502 501 367
286 100 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7732 8106 8252 387
Size: 1064 138 6143 374 146 1280
Fragments arranged by size:
6143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 3277 3405 3420 3449 4095 4271
Size: 393 628 1017 128 15 29 646 176
Cuts at: 4271 4783 5160 5434 5814 7208 7805 8592 8860
Size: 512 377 274 380 1394 597 787 268
Cuts at: 8860 1239
Size: 1524
Fragments arranged by size:
1524 1394 1017 787 646 628 597 512
393 380 377 274 268 176 128 29
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 2711
Size: 9145
EcoRI G'AATT_C
Cuts at: 4099 5587 6454 6832 4099
Size: 1488 867 378 6412
Fragments arranged by size:
6412 1488 867 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3690 4174 4516 6028 3690
Size: 484 342 1512 6807
Fragments arranged by size:
6807 1512 484 342
FspI TGC'GCA
Cuts at: 3044 7651 3044
Size: 4607 4538
HaeII r_GCGC'y
Cuts at: 1678 2098 8292 1678
Size: 420 6194 2531
Fragments arranged by size:
6194 2531 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 3640 3672 3911
Size: 32 135 32 563 32 1473 32 239
Cuts at: 3911 3943 4237 4269 5804 5836 6069 6101 6385
Size: 32 294 32 1535 32 233 32 284
Cuts at: 6385 6417 7786 7818 7860 7892 1373
Size: 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1535 1473 1369 563 294 284 239
233 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3659 4336 4890 5286 5538 6489
Size: 988 281 1721 677 554 396 252 951
Cuts at: 6489 6735 669
Size: 246 3079
Fragments arranged by size:
3079 1721 988 951 677 554 396 281
252 246
HindIII A'AGCT_T
Cuts at: 748 5176 6005 748
Size: 4428 829 3888
Fragments arranged by size:
4428 3888 829
HpaI GTT'AAC
Cuts at: 4336 6735 4336
Size: 2399 6746
KpnI G_GTAC'C
Cuts at: 6470 6470
Size: 9145
MfeI C'AATT_G
Cuts at: 6744 6744
Size: 9145
MluI A'CGCG_T
Cuts at: 9021 9021
Size: 9145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2583 6360 8259 8443 1305
Size: 1278 3777 1899 184 2007
Fragments arranged by size:
3777 2007 1899 1278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5559 5715 6051 10
Size: 54 1425 343 3727 156 336 3104
Fragments arranged by size:
3727 3104 1425 343 336 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 4262 4760 5434 5459 5507 7103
Size: 648 1284 1812 498 674 25 48 1596
Cuts at: 7103 7462 7621 518
Size: 359 159 2042
Fragments arranged by size:
2042 1812 1596 1284 674 648 498 359
159 48 25
MspA1I CmG'CkG
Cuts at: 1606 2058 6561 7238 8386 8631 1606
Size: 452 4503 677 1148 245 2120
Fragments arranged by size:
4503 2120 1148 677 452 245
NcoI C'CATG_G
Cuts at: 513 1065 6046 513
Size: 552 4981 3612
Fragments arranged by size:
4981 3612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9145
NotI GC'GGCC_GC
Cuts at: 6592 6592
Size: 9145
NruI TCG'CGA
Cuts at: 2363 2363
Size: 9145
NspI r_CATG'y
Cuts at: 1132 1229 3576 5512 6503 8048 1132
Size: 97 2347 1936 991 1545 2229
Fragments arranged by size:
2347 2229 1936 1545 991 97
PasI CC'CwG_GG
Cuts at: 1348 3069 1348
Size: 1721 7424
PciI A'CATG_T
Cuts at: 8044 8044
Size: 9145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5722 1108
Size: 4614 4531
PfoI T'CCnGG_A
Cuts at: 3488 4037 3488
Size: 549 8596
PmeI GTTT'AAAC
Cuts at: 6449 6449
Size: 9145
PmlI CAC'GTG
Cuts at: 5596 5596
Size: 9145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7214 7246 8727 8759 7214
Size: 32 1481 32 7600
Fragments arranged by size:
7600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9145
PsiI TTA'TAA
Cuts at: 2487 6715 2487
Size: 4228 4917
PstI C_TGCA'G
Cuts at: 830 6497 830
Size: 5667 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9145
PvuII CAG'CTG
Cuts at: 2058 2058
Size: 9145
SalI G'TCGA_C
Cuts at: 1936 6487 1936
Size: 4551 4594
SbfI CC_TGCA'GG
Cuts at: 6497 6497
Size: 9145
ScaI AGT'ACT
Cuts at: 4690 5758 7393 9105 4690
Size: 1068 1635 1712 4730
Fragments arranged by size:
4730 1712 1635 1068
SfcI C'TryA_G
Cuts at: 826 1045 3171 3911 5016 6493 7628 8309 8500
Size: 219 2126 740 1105 1477 1135 681 191
Cuts at: 8500 826
Size: 1471
Fragments arranged by size:
2126 1477 1471 1135 1105 740 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9145
SmaI CCC'GGG
Cuts at: 1872 6472 1872
Size: 4600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2866 4443 6367 7249 8150 8412
Size: 197 926 923 1577 1924 882 901 262
Cuts at: 8412 820
Size: 1553
Fragments arranged by size:
1924 1577 1553 926 923 901 882 262
197
SnaBI TAC'GTA
Cuts at: 493 6936 493
Size: 6443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9145
SphI G_CATG'C
Cuts at: 1132 3576 6503 1132
Size: 2444 2927 3774
Fragments arranged by size:
3774 2927 2444
SspI AAT'ATT
Cuts at: 5 52 3012 3762 3852 7069 5
Size: 47 2960 750 90 3217 2081
Fragments arranged by size:
3217 2960 2081 750 90 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9145
StyI C'CwwG_G
Cuts at: 513 1065 4339 4654 6046 6584 513
Size: 552 3274 315 1392 538 3074
Fragments arranged by size:
3274 3074 1392 552 538 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 7172 7189 7342 7527 7972 325
Size: 1255 856 4736 17 153 185 445 1498
Fragments arranged by size:
4736 1498 1255 856 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2293 2742 3076
Size: 274 80 33 51 1443 316 449 334
Cuts at: 3076 4688 5426 5756 7391 9103 96
Size: 1612 738 330 1635 1712 138
Fragments arranged by size:
1712 1635 1612 1443 738 449 334 330
316 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2755 2787 5185 5217 9105 9137 2755
Size: 32 2398 32 3888 32 2763
Fragments arranged by size:
3888 2763 2398 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6014 1316
Size: 4698 4447
XbaI T'CTAG_A
Cuts at: 2518 6481 2518
Size: 3963 5182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5458 6127 5458
Size: 669 8476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9145
XmaI C'CCGG_G
Cuts at: 1870 6470 1870
Size: 4600 4545
XmnI GAAnn'nnTTC
Cuts at: 3011 3265 3466 3979 4135 5994 7274 3011
Size: 254 201 513 156 1859 1280 4882
Fragments arranged by size:
4882 1859 1280 513 254 201 156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6953 276
Size: 53 83 186 936 5419 2468
Fragments arranged by size:
5419 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI EcoICRI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI