Restriction map for
pFN21AE2332
(Circular) (Six-base) MAPSORT of: pFN21AE2332.seq Check: 1376 from: 1 to: 6358
pFN21AE2332 6358 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4168 278
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 3701 1937
Size: 837 927 4594
Fragments arranged by size:
4594 927 837
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2477 2637 4421 5805 6073 1632
Size: 633 212 160 1784 1384 268 1917
Fragments arranged by size:
1917 1784 1384 633 268 212 160
AflII C'TTAA_G
Cuts at: 820 1017 3248 820
Size: 197 2231 3930
Fragments arranged by size:
3930 2231 197
AflIII A'CryG_T
Cuts at: 1203 2558 2893 3653 5257 6234 1203
Size: 1355 335 760 1604 977 1327
Fragments arranged by size:
1604 1355 1327 977 760 335
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 2533 5087 2533
Size: 2554 3804
AleI CACnn'nnGTG
Cuts at: 1166 3654 1166
Size: 2488 3870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 4101 5673 4101
Size: 1572 4786
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 2156 2275 2650 3104 3667 3893 4045 6154
Size: 663 119 375 454 563 226 152 2109
Cuts at: 6154 1493
Size: 1697
Fragments arranged by size:
2109 1697 663 563 454 375 226 152
119
AseI AT'TA_AT
Cuts at: 160 1032 2819 3233 4912 160
Size: 872 1787 414 1679 1606
Fragments arranged by size:
1787 1679 1606 872 414
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 3683 1870
Size: 73 1740 4545
Fragments arranged by size:
4545 1740 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3679 5134 618
Size: 325 217 627 947 945 1455 1842
Fragments arranged by size:
1842 1455 947 945 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3374 3677 1391
Size: 1983 303 4072
Fragments arranged by size:
4072 1983 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4549 4583 1903
Size: 34 2612 34 3678
Fragments arranged by size:
3678 2612 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 2950 1548
Size: 1402 4956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4969 136
Size: 107 122 71 631 605 3297 1525
Fragments arranged by size:
3297 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2726 3244 6353 2726
Size: 518 3109 2731
Fragments arranged by size:
3109 2731 518
BlpI GC'TnA_GC
Cuts at: 3775 3775
Size: 6358
Bme1580I G_kGCm'C
Cuts at: 1790 4419 5575 1790
Size: 2629 1156 2573
Fragments arranged by size:
2629 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4089 5047 442
Size: 416 53 83 399 24 2672 958 1753
Fragments arranged by size:
2672 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5018 1239
Size: 3779 2579
Bpu10I CC'TnA_GC
Cuts at: 2827 2827
Size: 6358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 4483 5348 5646 3047
Size: 1436 865 298 3759
Fragments arranged by size:
3759 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 3002 5021 882
Size: 617 1203 300 2019 2219
Fragments arranged by size:
2219 2019 1203 617 300
BsaAI yAC'GTr
Cuts at: 493 1204 2559 4149 493
Size: 711 1355 1590 2702
Fragments arranged by size:
2702 1590 1355 711
BsaBI GATnn'nnATC
Cuts at: 3000 3042 6352 3000
Size: 42 3310 3006
Fragments arranged by size:
3310 3006 42
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4165 4547 5182 275
Size: 53 83 186 936 2632 382 635 1451
Fragments arranged by size:
2632 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4791 5189 5463 5610 6021 4791
Size: 398 274 147 411 5128
Fragments arranged by size:
5128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6133
Fragments arranged by size:
6133 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6358
BseYI C'CCAG_C
Cuts at: 5561 6057 5561
Size: 496 5862
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5785
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4419 4504 5575 4419
Size: 85 1071 5202
Fragments arranged by size:
5202 1071 85
BsmI GAATG_Cn'
Cuts at: 3869 3962 3869
Size: 93 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3374 3677 4419 4504 5575 1391
Size: 399 1584 303 742 85 1071 2174
Fragments arranged by size:
2174 1584 1071 742 399 303 85
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 3713 844
Size: 1171 1698 3489
Fragments arranged by size:
3489 1698 1171
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4853 5027 65
Size: 1183 3605 174 1396
Fragments arranged by size:
3605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5002 5189 1700
Size: 110 3192 187 2869
Fragments arranged by size:
3192 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 4418 5430 4418
Size: 1012 5346
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2198 794
Size: 1404 4954
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2726 3244 3688 4055 4440 4457 5909 5995
Size: 754 518 444 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 754 518 444 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2775 2775
Size: 6358
Bsu36I CC'TnA_GG
Cuts at: 3573 3573
Size: 6358
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 4576
Fragments arranged by size:
4576 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3462 3874 4668 4688 3040
Size: 422 412 794 20 4710
Fragments arranged by size:
4710 794 422 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3189 3224 529
Size: 35 2625 35 3663
Fragments arranged by size:
3663 2625 35 35
DraI TTT'AAA
Cuts at: 2806 3662 2806
Size: 856 5502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2472 2839 3805
Size: 54 1425 50 275 18 642 367 966
Cuts at: 3805 4694 6323 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 966 889 642 367 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2146 3142 3549 4288 1353
Size: 793 996 407 739 3423
Fragments arranged by size:
3423 996 793 739 407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2933 4945 5319 5465 387
Size: 1064 138 1344 2012 374 146 1280
Fragments arranged by size:
2012 1344 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2477 2637 4421 5018 5805 6073
Size: 393 633 212 160 1784 597 787 268
Cuts at: 6073 1239
Size: 1524
Fragments arranged by size:
1784 1524 787 633 597 393 268 212
160
EcoNI CCTnn'n_nnAGG
Cuts at: 2100 2100
Size: 6358
EcoO109I rG'GnC_Cy
Cuts at: 2105 3120 3606 2105
Size: 1015 486 4857
Fragments arranged by size:
4857 1015 486
EcoRI G'AATT_C
Cuts at: 2275 3104 3667 4045 2275
Size: 829 563 378 4588
Fragments arranged by size:
4588 829 563 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2595 2595
Size: 6358
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 5505 1678
Size: 3827 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2519 2551 4999 5031 5073
Size: 32 135 32 947 32 2448 32 42
Cuts at: 5073 5105 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 947 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 2468 3957 2468
Size: 1489 4869
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2841 10
Size: 54 1425 343 1009 3527
Fragments arranged by size:
3527 1425 1009 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 3098 3456 3654 4316 4675 4834
Size: 648 1807 125 358 198 662 359 159
Cuts at: 4834 518
Size: 2042
Fragments arranged by size:
2042 1807 662 648 359 358 198 159
125
MspA1I CmG'CkG
Cuts at: 1606 3774 4451 5599 5844 1606
Size: 2168 677 1148 245 2120
Fragments arranged by size:
2168 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 4576
Fragments arranged by size:
4576 939 552 291
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 3657 3716 5261 1132
Size: 97 2428 59 1545 2229
Fragments arranged by size:
2428 2229 1545 97 59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 3653 5257 3653
Size: 1604 4754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2105 3120 3606 2105
Size: 1015 486 4857
Fragments arranged by size:
4857 1015 486
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 2141 2403 3928 2141
Size: 262 1525 4571
Fragments arranged by size:
4571 1525 262
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2949 2981 2949
Size: 32 6326
PstI C_TGCA'G
Cuts at: 830 3710 830
Size: 2880 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 2076 4606 6318 2076
Size: 2530 1712 2116
Fragments arranged by size:
2530 2116 1712
SfcI C'TryA_G
Cuts at: 826 1045 3548 3706 4841 5522 5713 826
Size: 219 2503 158 1135 681 191 1471
Fragments arranged by size:
2503 1471 1135 681 219 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3026 3248 4462 5363 5625 820
Size: 197 926 1083 222 1214 901 262 1553
Fragments arranged by size:
1553 1214 1083 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 2962 4282 5
Size: 47 2910 1320 2081
Fragments arranged by size:
2910 2081 1320 47
StuI AGG'CCT
Cuts at: 1517 2313 1517
Size: 796 5562
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 3123 3797 513
Size: 552 939 291 828 674 3074
Fragments arranged by size:
3074 939 828 674 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2752 4385 4402 4555 4740 5185 325
Size: 1255 1172 1633 17 153 185 445 1498
Fragments arranged by size:
1633 1498 1255 1172 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 4604 6316
Size: 274 80 33 51 1443 97 2530 1712
Cuts at: 6316 96
Size: 138
Fragments arranged by size:
2530 1712 1443 274 138 97 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3393 3425 6318 6350 3393
Size: 32 2893 32 3401
Fragments arranged by size:
3401 2893 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2394 3447 4487 2394
Size: 1053 1040 4265
Fragments arranged by size:
4265 1053 1040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4166 276
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI