Restriction map for
pFN21AE2330
(Circular) (Six-base) MAPSORT of: pFN21AE2330.seq Check: 699 from: 1 to: 8863
pFN21AE2330 8863 bp
With 182 enzymes: SgfI *
April 25, 2011 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5674 5674
Size: 8863
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6673 278
Size: 53 83 186 936 5137 2468
Fragments arranged by size:
5137 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6206 1937
Size: 4269 4594
Acc65I G'GTAC_C
Cuts at: 6184 6184
Size: 8863
AclI AA'CG_TT
Cuts at: 1563 6990 7363 1563
Size: 5427 373 3063
Fragments arranged by size:
5427 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2188 2282 6926 8310 8578 1632
Size: 366 190 94 4644 1384 268 1917
Fragments arranged by size:
4644 1917 1384 366 268 190 94
AflII C'TTAA_G
Cuts at: 820 1017 3500 820
Size: 197 2483 6183
Fragments arranged by size:
6183 2483 197
AflIII A'CryG_T
Cuts at: 1203 3838 7762 8739 1203
Size: 2635 3924 977 1327
Fragments arranged by size:
3924 2635 1327 977
AgeI A'CCGG_T
Cuts at: 7694 7694
Size: 8863
AhdI GACnn_n'nnGTC
Cuts at: 7592 7592
Size: 8863
AleI CACnn'nnGTG
Cuts at: 1166 3944 6140 1166
Size: 2778 2196 3889
Fragments arranged by size:
3889 2778 2196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8863
AlwNI CAG_nnn'CTG
Cuts at: 2817 3434 4414 6606 8178 2817
Size: 617 980 2192 1572 3502
Fragments arranged by size:
3502 2192 1572 980 617
ApaI G_GGCC'C
Cuts at: 5572 5572
Size: 8863
ApaLI G'TGCA_C
Cuts at: 6920 8076 6920
Size: 1156 7707
ApoI r'AATT_y
Cuts at: 1493 2537 2789 2802 2902 2921 2945 3072 3405
Size: 1044 252 13 100 19 24 127 333
Cuts at: 3405 3732 5025 5342 5705 6172 6398 6550 8659
Size: 327 1293 317 363 467 226 152 2109
Cuts at: 8659 1493
Size: 1697
Fragments arranged by size:
2109 1697 1293 1044 467 363 333 327
317 252 226 152 127 100 24 19
13
AseI AT'TA_AT
Cuts at: 160 1032 4493 7417 160
Size: 872 3461 2924 1606
Fragments arranged by size:
3461 2924 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8863
AvaI C'yCGr_G
Cuts at: 1870 1943 6114 6188 1870
Size: 73 4171 74 4545
Fragments arranged by size:
4545 4171 74 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2182 2215 2825 2858 4845 4878 2182
Size: 33 610 33 1987 33 6167
Fragments arranged by size:
6167 1987 610 33 33 33
BamHI G'GATC_C
Cuts at: 2488 5248 5994 6119 6193 6560 2488
Size: 2760 746 125 74 367 4791
Fragments arranged by size:
4791 2760 746 367 125 74
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2217 6184 7639 618
Size: 325 217 627 430 3967 1455 1842
Fragments arranged by size:
3967 1842 1455 627 430 325 217
BanII G_rGCy'C
Cuts at: 1391 4247 5544 5572 6182 1391
Size: 2856 1297 28 610 4072
Fragments arranged by size:
4072 2856 1297 610 28
BbeI G_GCGC'C
Cuts at: 2221 2221
Size: 8863
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2052 4076 4335 5720 928
Size: 971 153 2024 259 1385 4071
Fragments arranged by size:
4071 2024 1385 971 259 153
BbvCI CC'TCA_GC
Cuts at: 3913 3913
Size: 8863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7054 7088 1903
Size: 34 5117 34 3678
Fragments arranged by size:
5117 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5679 6755 7965 3875
Size: 1804 1076 1210 4773
Fragments arranged by size:
4773 1804 1210 1076
BclI T'GATC_A
Cuts at: 1548 4186 6058 1548
Size: 2638 1872 4353
Fragments arranged by size:
4353 2638 1872
BfrBI ATG'CAT
Cuts at: 3050 3050
Size: 8863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4899 5276 7474
Size: 107 122 71 631 605 3227 377 2198
Cuts at: 7474 136
Size: 1525
Fragments arranged by size:
3227 2198 1525 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 2697 8858 2697
Size: 6161 2702
BlpI GC'TnA_GC
Cuts at: 2509 4119 6280 2509
Size: 1610 2161 5092
Fragments arranged by size:
5092 2161 1610
Bme1580I G_kGCm'C
Cuts at: 1790 2982 5572 6924 8080 1790
Size: 1192 2590 1352 1156 2573
Fragments arranged by size:
2590 2573 1352 1192 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2656 6594 7552
Size: 416 53 83 399 24 1239 3938 958
Cuts at: 7552 442
Size: 1753
Fragments arranged by size:
3938 1753 1239 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4125 5632 4125
Size: 1507 7356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2858 4337 4527 5039 5130 5745 6053
Size: 910 709 1479 190 512 91 615 308
Cuts at: 6053 7523 1239
Size: 1470 2579
Fragments arranged by size:
2579 1479 1470 910 709 615 512 308
190 91
Bpu10I CC'TnA_GC
Cuts at: 3913 3913
Size: 8863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 4851 6988 7853 8151 2753
Size: 2098 2137 865 298 3465
Fragments arranged by size:
3465 2137 2098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2346 7526 882
Size: 617 847 5180 2219
Fragments arranged by size:
5180 2219 847 617
BsaAI yAC'GTr
Cuts at: 493 1204 6654 493
Size: 711 5450 2702
Fragments arranged by size:
5450 2702 711
BsaBI GATnn'nnATC
Cuts at: 2696 3027 8857 2696
Size: 331 5830 2702
Fragments arranged by size:
5830 2702 331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2218 6670 7052 7687
Size: 53 83 186 936 685 4452 382 635
Cuts at: 7687 275
Size: 1451
Fragments arranged by size:
4452 1451 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7296 7694 7968 8115 8526 7296
Size: 398 274 147 411 7633
Fragments arranged by size:
7633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5571 5601 5925 5955 5985
Size: 30 165 30 4168 30 324 30 30
Cuts at: 5985 1178
Size: 4056
Fragments arranged by size:
4168 4056 324 165 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 2091 2420 3452 5592 5946 5976 1186
Size: 896 9 329 1032 2140 354 30 4073
Fragments arranged by size:
4073 2140 1032 896 354 329 30 9
BseYI C'CCAG_C
Cuts at: 8066 8562 8066
Size: 496 8367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3447 3919 5702 5969 1163
Size: 573 1711 472 1783 267 4057
Fragments arranged by size:
4057 1783 1711 573 472 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6313 7074 8102 1540
Size: 275 186 4312 761 1028 2301
Fragments arranged by size:
4312 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5047 6924 7009 8080 5047
Size: 1877 85 1071 5830
Fragments arranged by size:
5830 1877 1071 85
BsmI GAATG_Cn'
Cuts at: 5348 5742 6374 6467 5348
Size: 394 632 93 7744
Fragments arranged by size:
7744 632 394 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2982 4247 5047 5544 5572 6182 6924
Size: 399 1192 1265 800 497 28 610 742
Cuts at: 6924 7009 8080 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1265 1192 1071 800 742 610 497
399 85 28
BspEI T'CCGG_A
Cuts at: 8526 8526
Size: 8863
BspHI T'CATG_A
Cuts at: 4015 6752 8482 4015
Size: 2737 1730 4396
Fragments arranged by size:
4396 2737 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 5674 6218 844
Size: 1520 3310 544 3489
Fragments arranged by size:
3489 3310 1520 544
BsrBI CCG'CTC
Cuts at: 1394 1823 2443 3115 6750 1394
Size: 429 620 672 3635 3507
Fragments arranged by size:
3635 3507 672 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4746 5262 5655 7358 7532 65
Size: 1183 3498 516 393 1703 174 1396
Fragments arranged by size:
3498 1703 1396 1183 516 393 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7507 7694 1700
Size: 110 5697 187 2869
Fragments arranged by size:
5697 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2893 5788 96
Size: 2797 2895 3171
Fragments arranged by size:
3171 2895 2797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8863
BssSI C'ACGA_G
Cuts at: 3116 5855 6923 7935 3116
Size: 2739 1068 1012 4044
Fragments arranged by size:
4044 2739 1068 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8863
BstBI TT'CG_AA
Cuts at: 7667 7667
Size: 8863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8863
BstYI r'GATC_y
Cuts at: 1972 2381 2488 2697 5248 5994 6119 6193 6560
Size: 409 107 209 2551 746 125 74 367
Cuts at: 6560 6945 6962 8414 8500 8512 8858 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2551 1977 1452 746 409 385 367 346
209 125 107 86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2612 4334 2612
Size: 1722 7141
BtgI C'CryG_G
Cuts at: 513 1065 3876 3939 5193 513
Size: 552 2811 63 1254 4183
Fragments arranged by size:
4183 2811 1254 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8124
Fragments arranged by size:
8124 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 4386 6379 7173 7193 2565
Size: 1821 1993 794 20 4235
Fragments arranged by size:
4235 1993 1821 794 20
ClaI AT'CG_AT
Cuts at: 1554 4642 4770 4791 1554
Size: 3088 128 21 5626
Fragments arranged by size:
5626 3088 128 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2029 2064 4729 4764 529
Size: 35 1465 35 2665 35 4628
Fragments arranged by size:
4628 2665 1465 35 35 35
DraI TTT'AAA
Cuts at: 4965 6167 4965
Size: 1202 7661
DraIII CAC_nnn'GTG
Cuts at: 3688 3688
Size: 8863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7870 809
Size: 507 6554 1802
Fragments arranged by size:
6554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2450 5481 6310
Size: 54 1425 50 275 18 620 3031 829
Cuts at: 6310 7199 8828 8
Size: 889 1629 43
Fragments arranged by size:
3031 1629 1425 889 829 620 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6310 1537
Size: 275 4498 4090
Fragments arranged by size:
4498 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 2349 2748 3249 4677 5106 5458 5601
Size: 715 281 399 501 1428 429 352 143
Cuts at: 5601 6793 1353
Size: 1192 3423
Fragments arranged by size:
3423 1428 1192 715 501 429 399 352
281 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 7450 7824 7970 387
Size: 1064 138 2723 3138 374 146 1280
Fragments arranged by size:
3138 2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2149 2188 2282 2858 4337 4527
Size: 393 366 151 39 94 576 1479 190
Cuts at: 4527 5039 5130 5745 6053 6926 7523 8310 8578
Size: 512 91 615 308 873 597 787 268
Cuts at: 8578 1239
Size: 1524
Fragments arranged by size:
1524 1479 873 787 615 597 576 512
393 366 308 268 190 151 94 91
39
EcoNI CCTnn'n_nnAGG
Cuts at: 3248 4713 3248
Size: 1465 7398
EcoO109I rG'GnC_Cy
Cuts at: 5568 5568
Size: 8863
EcoRI G'AATT_C
Cuts at: 2802 2902 6172 6550 2802
Size: 100 3270 378 5115
Fragments arranged by size:
5115 3270 378 100
EcoRV GAT'ATC
Cuts at: 5643 5643
Size: 8863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2238 2312 2238
Size: 74 8789
FspI TGC'GCA
Cuts at: 7369 7369
Size: 8863
HaeII r_GCGC'y
Cuts at: 1678 2221 8010 1678
Size: 543 5789 2531
Fragments arranged by size:
5789 2531 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 3242 3274 3614
Size: 32 135 32 794 32 844 32 340
Cuts at: 3614 3646 3853 3885 4052 4066 4084 4098 4828
Size: 32 207 32 167 14 18 14 730
Cuts at: 4828 4860 5571 5603 5868 5900 6111 6143 7504
Size: 32 711 32 265 32 211 32 1361
Cuts at: 7504 7536 7578 7610 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1361 844 794 730 711 340 265
211 207 167 135 42 32 32 32
32 32 32 32 32 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 6207 6453 669
Size: 988 281 775 3494 246 3079
Fragments arranged by size:
3494 3079 988 775 281 246
HindIII A'AGCT_T
Cuts at: 748 2722 4283 748
Size: 1974 1561 5328
Fragments arranged by size:
5328 1974 1561
HpaI GTT'AAC
Cuts at: 6453 6453
Size: 8863
KasI G'GCGC_C
Cuts at: 2217 2217
Size: 8863
KpnI G_GTAC'C
Cuts at: 6188 6188
Size: 8863
MfeI C'AATT_G
Cuts at: 4209 6138 6462 4209
Size: 1929 324 6610
Fragments arranged by size:
6610 1929 324
MluI A'CGCG_T
Cuts at: 3838 8739 3838
Size: 4901 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2977 3240 5972 7977 8161 1305
Size: 1672 263 2732 2005 184 2007
Fragments arranged by size:
2732 2007 2005 1672 263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2452 5483 10
Size: 54 1425 343 620 3031 3390
Fragments arranged by size:
3390 3031 1425 620 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 3033 3944 6140 6821 7180 7339
Size: 648 943 924 911 2196 681 359 159
Cuts at: 7339 518
Size: 2042
Fragments arranged by size:
2196 2042 943 924 911 681 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2456 5008 5241 5753 5917 6279 6956 8104
Size: 850 2552 233 512 164 362 677 1148
Cuts at: 8104 8349 1606
Size: 245 2120
Fragments arranged by size:
2552 2120 1148 850 677 512 362 245
233 164
NarI GG'CG_CC
Cuts at: 2218 2218
Size: 8863
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8311
NdeI CA'TA_TG
Cuts at: 387 2310 387
Size: 1923 6940
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8863
NotI GC'GGCC_GC
Cuts at: 6310 6310
Size: 8863
NsiI A_TGCA'T
Cuts at: 3052 3052
Size: 8863
NspI r_CATG'y
Cuts at: 1132 1229 3551 6221 7766 1132
Size: 97 2322 2670 1545 2229
Fragments arranged by size:
2670 2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5304 1348
Size: 3956 4907
PciI A'CATG_T
Cuts at: 7762 7762
Size: 8863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6022 1108
Size: 4914 3949
PfoI T'CCnGG_A
Cuts at: 4353 6069 6122 4353
Size: 1716 53 7094
Fragments arranged by size:
7094 1716 53
PmeI GTTT'AAAC
Cuts at: 6167 6167
Size: 8863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 2489 6932 6964 8445 8477 2457
Size: 32 4443 32 1481 32 2843
Fragments arranged by size:
4443 2843 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8863
PsiI TTA'TAA
Cuts at: 4733 5433 6433 4733
Size: 700 1000 7163
Fragments arranged by size:
7163 1000 700
PspOMI G'GGCC_C
Cuts at: 5568 5568
Size: 8863
PspXI vC'TCGA_Gb
Cuts at: 6114 6114
Size: 8863
PstI C_TGCA'G
Cuts at: 830 4717 5444 5475 5897 6215 830
Size: 3887 727 31 422 318 3478
Fragments arranged by size:
3887 3478 727 422 318 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8863
PvuII CAG'CTG
Cuts at: 2456 5008 5753 2456
Size: 2552 745 5566
Fragments arranged by size:
5566 2552 745
SalI G'TCGA_C
Cuts at: 1936 6205 1936
Size: 4269 4594
SapI GCTCTTCn'nnn_
Cuts at: 2349 4677 2349
Size: 2328 6535
SbfI CC_TGCA'GG
Cuts at: 4717 6215 4717
Size: 1498 7365
ScaI AGT'ACT
Cuts at: 5722 7111 8823 5722
Size: 1389 1712 5762
Fragments arranged by size:
5762 1712 1389
SexAI A'CCwGG_T
Cuts at: 3684 4165 3684
Size: 481 8382
SfcI C'TryA_G
Cuts at: 826 1045 4604 4713 5440 5471 5893 6211 7346
Size: 219 3559 109 727 31 422 318 1135
Cuts at: 7346 8027 8218 826
Size: 681 191 1471
Fragments arranged by size:
3559 1471 1135 727 681 422 318 219
191 109 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 4899 4899
Size: 8863
SfoI GGC'GCC
Cuts at: 2219 2219
Size: 8863
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8863
SmaI CCC'GGG
Cuts at: 1872 6190 1872
Size: 4318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 3500 4866 6114 6967 7868
Size: 197 926 825 732 1366 1248 853 901
Cuts at: 7868 8130 820
Size: 262 1553
Fragments arranged by size:
1553 1366 1248 926 901 853 825 732
262 197
SnaBI TAC'GTA
Cuts at: 493 6654 493
Size: 6161 2702
SpeI A'CTAG_T
Cuts at: 152 5870 152
Size: 5718 3145
SphI G_CATG'C
Cuts at: 1132 6221 1132
Size: 5089 3774
SspI AAT'ATT
Cuts at: 5 52 6787 5
Size: 47 6735 2081
Fragments arranged by size:
6735 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 2394 3194 4502 1517
Size: 645 232 800 1308 5878
Fragments arranged by size:
5878 1308 800 645 232
StyI C'CwwG_G
Cuts at: 513 1065 5572 6302 513
Size: 552 4507 730 3074
Fragments arranged by size:
4507 3074 730 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4671 6890 6907 7060 7245 7690 325
Size: 1255 3091 2219 17 153 185 445 1498
Fragments arranged by size:
3091 2219 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2893 3544 5405
Size: 274 80 33 51 1443 916 651 1861
Cuts at: 5405 5720 5788 7109 8821 96
Size: 315 68 1321 1712 138
Fragments arranged by size:
1861 1712 1443 1321 916 651 315 274
138 80 68 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3718 3750 4994 5026 5925 5957 8823 8855 3718
Size: 32 1244 32 899 32 2866 32 3726
Fragments arranged by size:
3726 2866 1244 899 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8863
XbaI T'CTAG_A
Cuts at: 2063 4846 6199 2063
Size: 2783 1353 4727
Fragments arranged by size:
4727 2783 1353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4834 5579 4834
Size: 745 8118
XhoI C'TCGA_G
Cuts at: 1943 6114 1943
Size: 4171 4692
XmaI C'CCGG_G
Cuts at: 1870 6188 1870
Size: 4318 4545
XmnI GAAnn'nnTTC
Cuts at: 2802 3660 6992 2802
Size: 858 3332 4673
Fragments arranged by size:
4673 3332 858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6671 276
Size: 53 83 186 936 5137 2468
Fragments arranged by size:
5137 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BsiWI BsmBI BstXI BstZ17I
EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PpuMI PsrI RsrII SacI SacII SanDI SrfI SwaI