Restriction map for
pFN21AE2327
(Circular) (Six-base) MAPSORT of: pFN21AE2327.seq Check: 850 from: 1 to: 7642
pFN21AE2327 7642 bp
With 182 enzymes: SgfI *
February 11, 2011 18:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4818 4985 1937
Size: 2881 167 4594
Fragments arranged by size:
4594 2881 167
Acc65I G'GTAC_C
Cuts at: 4963 4963
Size: 7642
AclI AA'CG_TT
Cuts at: 1563 3328 5769 6142 1563
Size: 1765 2441 373 3063
Fragments arranged by size:
3063 2441 1765 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 2936 5705 7089 7357 1632
Size: 771 533 2769 1384 268 1917
Fragments arranged by size:
2769 1917 1384 771 533 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 6541 7518 1203
Size: 5338 977 1327
Fragments arranged by size:
5338 1327 977
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4156 1184
Size: 2972 4670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2902 2934 2902
Size: 32 7610
AlwNI CAG_nnn'CTG
Cuts at: 2299 4297 4845 5385 6957 2299
Size: 1998 548 540 1572 2984
Fragments arranged by size:
2984 1998 1572 548 540
ApaLI G'TGCA_C
Cuts at: 5699 6855 5699
Size: 1156 6486
ApoI r'AATT_y
Cuts at: 1493 2302 2656 2883 3421 3557 3894 4387 4452
Size: 809 354 227 538 136 337 493 65
Cuts at: 4452 4462 4951 5177 5329 7438 1493
Size: 10 489 226 152 2109 1697
Fragments arranged by size:
2109 1697 809 538 493 489 354 337
227 226 152 136 65 10
AseI AT'TA_AT
Cuts at: 160 1032 3977 6196 160
Size: 872 2945 2219 1606
Fragments arranged by size:
2945 2219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 4967 1870
Size: 73 222 2802 4545
Fragments arranged by size:
4545 2802 222 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2370 2403 2370
Size: 33 7609
BamHI G'GATC_C
Cuts at: 4972 5339 4972
Size: 367 7275
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 4963 6418 618
Size: 325 217 627 382 2794 1455 1842
Fragments arranged by size:
2794 1842 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 4961 1391
Size: 3570 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3860 4269 928
Size: 971 1961 409 4301
Fragments arranged by size:
4301 1961 971 409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5833 5867 1903
Size: 34 3896 34 3678
Fragments arranged by size:
3896 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2366 5534 6744 2366
Size: 3168 1210 3264
Fragments arranged by size:
3264 3168 1210
BclI T'GATC_A
Cuts at: 1548 4875 1548
Size: 3327 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7637 7637
Size: 7642
BlpI GC'TnA_GC
Cuts at: 4323 5059 4323
Size: 736 6906
Bme1580I G_kGCm'C
Cuts at: 1790 2172 5703 6859 1790
Size: 382 3531 1156 2573
Fragments arranged by size:
3531 2573 1156 382
BmgBI CAC'GTC
Cuts at: 1318 4232 1318
Size: 2914 4728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 4148 4217
Size: 416 53 83 399 24 621 2110 69
Cuts at: 4217 5373 6331 442
Size: 1156 958 1753
Fragments arranged by size:
2110 1753 1156 958 621 416 399 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2876 2945 4716 6302 1239
Size: 1637 69 1771 1586 2579
Fragments arranged by size:
2579 1771 1637 1586 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 5767 6632 6930 2703
Size: 3064 865 298 3415
Fragments arranged by size:
3415 3064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6305 882
Size: 617 4806 2219
Fragments arranged by size:
4806 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5433 493
Size: 711 4229 2702
Fragments arranged by size:
4229 2702 711
BsaBI GATnn'nnATC
Cuts at: 2670 3879 4686 7636 2670
Size: 1209 807 2950 2676
Fragments arranged by size:
2950 2676 1209 807
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5449 5831 6466 275
Size: 53 83 186 936 3916 382 635 1451
Fragments arranged by size:
3916 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2057 2111 3178 6075 6473 6747 6894 7305 2057
Size: 54 1067 2897 398 274 147 411 2394
Fragments arranged by size:
2897 2394 1067 411 398 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 1178
Size: 30 165 30 690 30 6697
Fragments arranged by size:
6697 690 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 1186
Size: 944 6698
BseYI C'CCAG_C
Cuts at: 3495 4212 4260 6845 7341 3495
Size: 717 48 2585 496 3796
Fragments arranged by size:
3796 2585 717 496 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4606 4869 1163
Size: 573 2870 263 3936
Fragments arranged by size:
3936 2870 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4135 5703 5788 6859 4135
Size: 1568 85 1071 4918
Fragments arranged by size:
4918 1568 1071 85
BsmI GAATG_Cn'
Cuts at: 2643 3118 5153 5246 2643
Size: 475 2035 93 5039
Fragments arranged by size:
5039 2035 475 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2894 3719 2894
Size: 825 6817
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 4135 4961 5703 5788 6859 1391
Size: 399 382 1963 826 742 85 1071 2174
Fragments arranged by size:
2174 1963 1071 826 742 399 382 85
BspEI T'CCGG_A
Cuts at: 2111 7305 2111
Size: 5194 2448
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4997 844
Size: 4153 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5529 1394
Size: 429 3706 3507
Fragments arranged by size:
3706 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3660 3832 6137 6311 65
Size: 1183 2412 172 2305 174 1396
Fragments arranged by size:
2412 2305 1396 1183 174 172
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 6286 6473 1700
Size: 110 347 4129 187 2869
Fragments arranged by size:
4129 2869 347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2290 2933 96
Size: 2194 643 4805
Fragments arranged by size:
4805 2194 643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4297 4742 794
Size: 3503 445 3694
Fragments arranged by size:
3694 3503 445
BstBI TT'CG_AA
Cuts at: 6446 6446
Size: 7642
BstEII G'GTnAC_C
Cuts at: 1184 2099 1184
Size: 915 6727
BstXI CCAn_nnnn'nTGG
Cuts at: 4212 4212
Size: 7642
BstYI r'GATC_y
Cuts at: 1972 2765 4189 4369 4972 5339 5724 5741 7193
Size: 793 1424 180 603 367 385 17 1452
Cuts at: 7193 7279 7291 7637 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1424 793 603 385 367 346
180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2361 2361
Size: 7642
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7090
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 4008 4286 5158 5952 5972 2866
Size: 1142 278 872 794 20 4536
Fragments arranged by size:
4536 1142 872 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7607
DraI TTT'AAA
Cuts at: 3547 3616 4946 3547
Size: 69 1330 6243
Fragments arranged by size:
6243 1330 69
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6649 809
Size: 507 5333 1802
Fragments arranged by size:
5333 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3332 5089 5978
Size: 54 1425 50 275 18 1502 1757 889
Cuts at: 5978 7607 8
Size: 1629 43
Fragments arranged by size:
1757 1629 1502 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3456 3811 4212 5572 1353
Size: 2103 355 401 1360 3423
Fragments arranged by size:
3423 2103 1360 401 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2145 4902 6229 6603 6749 387
Size: 1064 138 556 2757 1327 374 146 1280
Fragments arranged by size:
2757 1327 1280 1064 556 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2876 2936 2945 4716 5705 6302
Size: 393 771 473 60 9 1771 989 597
Cuts at: 6302 7089 7357 1239
Size: 787 268 1524
Fragments arranged by size:
1771 1524 989 787 771 597 473 393
268 60 9
EcoO109I rG'GnC_Cy
Cuts at: 2045 2045
Size: 7642
EcoRI G'AATT_C
Cuts at: 2302 4951 5329 2302
Size: 2649 378 4615
Fragments arranged by size:
4615 2649 378
EcoRV GAT'ATC
Cuts at: 2346 2879 2346
Size: 533 7109
FspI TGC'GCA
Cuts at: 6148 6148
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 6789 1678
Size: 5111 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 3790 3822 3974
Size: 32 135 32 743 32 1443 32 152
Cuts at: 3974 4006 4361 4393 4789 4820 4821 4852 6283
Size: 32 355 32 396 31 1 31 1431
Cuts at: 6283 6315 6357 6389 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 1431 743 396 355 152 135
42 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3598 4986 5232 669
Size: 988 281 1660 1388 246 3079
Fragments arranged by size:
3079 1660 1388 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 3598 5232 3598
Size: 1634 6008
KpnI G_GTAC'C
Cuts at: 4967 4967
Size: 7642
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6756 6940 1305
Size: 5451 184 2007
Fragments arranged by size:
5451 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3334 10
Size: 54 1425 343 1502 4318
Fragments arranged by size:
4318 1502 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2265 5600 5959 6118 518
Size: 648 1099 3335 359 159 2042
Fragments arranged by size:
3335 2042 1099 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3767 5058 5735 6883 7128 1606
Size: 2161 1291 677 1148 245 2120
Fragments arranged by size:
2161 2120 1291 1148 677 245
NaeI GCC'GGC
Cuts at: 2159 2159
Size: 7642
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7090
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NgoMIV G'CCGG_C
Cuts at: 2157 2157
Size: 7642
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 2197 3284 5000 6545 1132
Size: 97 968 1087 1716 1545 2229
Fragments arranged by size:
2229 1716 1545 1087 968 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7642
PciI A'CATG_T
Cuts at: 6541 6541
Size: 7642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4109 1108
Size: 3001 4641
PfoI T'CCnGG_A
Cuts at: 2430 4923 2430
Size: 2493 5149
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2045 2045
Size: 7642
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7642
PsiI TTA'TAA
Cuts at: 5212 5212
Size: 7642
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2274 2306 3909 3941 2274
Size: 32 1603 32 5975
Fragments arranged by size:
5975 1603 32 32
PstI C_TGCA'G
Cuts at: 830 2839 3030 4293 4994 830
Size: 2009 191 1263 701 3478
Fragments arranged by size:
3478 2009 1263 701 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SanDI GG'GwC_CC
Cuts at: 2045 2045
Size: 7642
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 2872 3376 4158 4854 5890 7602 2872
Size: 504 782 696 1036 1712 2912
Fragments arranged by size:
2912 1712 1036 782 696 504
SexAI A'CCwGG_T
Cuts at: 3163 3826 3163
Size: 663 6979
SfcI C'TryA_G
Cuts at: 826 1045 2253 2835 3026 3378 3591 4289 4704
Size: 219 1208 582 191 352 213 698 415
Cuts at: 4704 4815 4990 6125 6806 6997 826
Size: 111 175 1135 681 191 1471
Fragments arranged by size:
1471 1208 1135 698 681 582 415 352
219 213 191 191 175 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 2167 4969 1872
Size: 295 2802 4545
Fragments arranged by size:
4545 2802 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 5746 6647 6909 820
Size: 197 926 775 3028 901 262 1553
Fragments arranged by size:
3028 1553 926 901 775 262 197
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 5000 1132
Size: 3868 3774
SspI AAT'ATT
Cuts at: 5 52 3093 3134 4268 5566 5
Size: 47 3041 41 1134 1298 2081
Fragments arranged by size:
3041 2081 1298 1134 47 41
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7642
StyI C'CwwG_G
Cuts at: 513 1065 2906 5081 513
Size: 552 1841 2175 3074
Fragments arranged by size:
3074 2175 1841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2511 5669 5686 5839 6024 6469 325
Size: 1255 931 3158 17 153 185 445 1498
Fragments arranged by size:
3158 1498 1255 931 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 2290 2786
Size: 274 80 33 51 1443 280 33 496
Cuts at: 2786 2870 2933 3374 3755 4156 4852 5888 7600
Size: 84 63 441 381 401 696 1036 1712
Cuts at: 7600 96
Size: 138
Fragments arranged by size:
1712 1443 1036 696 496 441 401 381
280 274 138 84 80 63 51 33
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7602 7634 7602
Size: 32 7610
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7642
XbaI T'CTAG_A
Cuts at: 3871 4978 3871
Size: 1107 6535
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7642
XmaI C'CCGG_G
Cuts at: 1870 2165 4967 1870
Size: 295 2802 4545
Fragments arranged by size:
4545 2802 295
XmnI GAAnn'nnTTC
Cuts at: 3364 4269 5771 3364
Size: 905 1502 5235
Fragments arranged by size:
5235 1502 905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PvuII RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI