Restriction map for
pFN21AE2325
(Circular) (Six-base) MAPSORT of: pFN21AE2325.seq Check: 7103 from: 1 to: 10636
pFN21AE2325 10636 bp
With 182 enzymes: SgfI *
June 20, 2011 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2323 2323
Size: 10636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8446 278
Size: 53 83 186 936 6910 2468
Fragments arranged by size:
6910 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3593 6234 7979 1937
Size: 1656 2641 1745 4594
Fragments arranged by size:
4594 2641 1745 1656
Acc65I G'GTAC_C
Cuts at: 7957 7957
Size: 10636
AclI AA'CG_TT
Cuts at: 1563 8763 9136 1563
Size: 7200 373 3063
Fragments arranged by size:
7200 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2837 6624 7293 8699 10083 10351 1632
Size: 1085 120 3787 669 1406 1384 268 1917
Fragments arranged by size:
3787 1917 1406 1384 1085 669 268 120
AflII C'TTAA_G
Cuts at: 820 1017 7684 820
Size: 197 6667 3772
Fragments arranged by size:
6667 3772 197
AflIII A'CryG_T
Cuts at: 1203 6267 7002 7230 9535 10512 1203
Size: 5064 735 228 2305 977 1327
Fragments arranged by size:
5064 2305 1327 977 735 228
AgeI A'CCGG_T
Cuts at: 5331 9467 5331
Size: 4136 6500
AhdI GACnn_n'nnGTC
Cuts at: 9365 9365
Size: 10636
AleI CACnn'nnGTG
Cuts at: 1166 2993 3552 4108 4583 1166
Size: 1827 559 556 475 7219
Fragments arranged by size:
7219 1827 559 556 475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 1184
Size: 1011 9625
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4452 4484 7553 7585 4452
Size: 32 3069 32 7503
Fragments arranged by size:
7503 3069 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3115 3338 3937 5399 8379 9951 3115
Size: 223 599 1462 2980 1572 3800
Fragments arranged by size:
3800 2980 1572 1462 599 223
ApaLI G'TGCA_C
Cuts at: 2404 8693 9849 2404
Size: 6289 1156 3191
Fragments arranged by size:
6289 3191 1156
ApoI r'AATT_y
Cuts at: 1493 2543 2612 2836 3269 3710 4130 4166 4283
Size: 1050 69 224 433 441 420 36 117
Cuts at: 4283 4309 4994 5410 5992 7255 7945 8171 8323
Size: 26 685 416 582 1263 690 226 152
Cuts at: 8323 10432 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1263 1050 690 685 582 441
433 420 416 226 224 152 117 69
36 26
AseI AT'TA_AT
Cuts at: 160 1032 6996 9190 160
Size: 872 5964 2194 1606
Fragments arranged by size:
5964 2194 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10636
AvaI C'yCGr_G
Cuts at: 1870 1943 7961 1870
Size: 73 6018 4545
Fragments arranged by size:
6018 4545 73
AvrII C'CTAG_G
Cuts at: 2623 2623
Size: 10636
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2977 3010 2977
Size: 33 10603
BamHI G'GATC_C
Cuts at: 2927 4198 4366 6858 7399 7476 7966 8333 2927
Size: 1271 168 2492 541 77 490 367 5230
Fragments arranged by size:
5230 2492 1271 541 490 367 168 77
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5591 7957 9412 618
Size: 325 217 627 3804 2366 1455 1842
Fragments arranged by size:
3804 2366 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 3173 3413 3764 4440 4567 4880 7955
Size: 835 947 240 351 676 127 313 3075
Cuts at: 7955 1391
Size: 4072
Fragments arranged by size:
4072 3075 947 835 676 351 313 240
127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2705 2901 4489 4594 4920 5193 7908
Size: 971 806 196 1588 105 326 273 2715
Cuts at: 7908 928
Size: 3656
Fragments arranged by size:
3656 2715 1588 971 806 326 273 196
105
BbvCI CC'TCA_GC
Cuts at: 3315 5280 3315
Size: 1965 8671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6065 6099 8827 8861 1903
Size: 34 4128 34 2728 34 3678
Fragments arranged by size:
4128 3678 2728 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8528 9738 8528
Size: 1210 9426
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9247 136
Size: 107 122 71 631 605 7575 1525
Fragments arranged by size:
7575 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4210 5056 5664 5815 10631 4210
Size: 846 608 151 4816 4215
Fragments arranged by size:
4816 4215 846 608 151
BlpI GC'TnA_GC
Cuts at: 3907 5582 8053 3907
Size: 1675 2471 6490
Fragments arranged by size:
6490 2471 1675
Bme1580I G_kGCm'C
Cuts at: 1790 2408 3777 3950 5596 8697 9853 1790
Size: 618 1369 173 1646 3101 1156 2573
Fragments arranged by size:
3101 2573 1646 1369 1156 618 173
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3100 7348 8367
Size: 416 53 83 399 24 1683 4248 1019
Cuts at: 8367 9325 442
Size: 958 1753
Fragments arranged by size:
4248 1753 1683 1019 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 7085 1056
Size: 6029 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 3454 4919 5397 5669 6669 7271 9296
Size: 1010 1205 1465 478 272 1000 602 2025
Cuts at: 9296 1239
Size: 2579
Fragments arranged by size:
2579 2025 1465 1205 1010 1000 602 478
272
Bpu10I CC'TnA_GC
Cuts at: 3315 5280 3315
Size: 1965 8671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5114 7777 8761 9626 9924 5114
Size: 2663 984 865 298 5826
Fragments arranged by size:
5826 2663 984 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3892 4754 7010 9299 882
Size: 617 2393 862 2256 2289 2219
Fragments arranged by size:
2393 2289 2256 2219 862 617
BsaAI yAC'GTr
Cuts at: 493 1204 4991 8427 493
Size: 711 3787 3436 2702
Fragments arranged by size:
3787 3436 2702 711
BsaBI GATnn'nnATC
Cuts at: 4541 10630 4541
Size: 6089 4547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8443 8825 9460 275
Size: 53 83 186 936 6910 382 635 1451
Fragments arranged by size:
6910 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5331 7473 9069 9467 9741 9888 10299 5331
Size: 2142 1596 398 274 147 411 5668
Fragments arranged by size:
5668 2142 1596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6642 6672 7816 7846 1178
Size: 30 165 30 5239 30 1144 30 3968
Fragments arranged by size:
5239 3968 1144 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5332 7395 1186
Size: 4146 2063 4427
Fragments arranged by size:
4427 4146 2063
BseYI C'CCAG_C
Cuts at: 3048 5219 6433 7068 9839 10335 3048
Size: 2171 1214 635 2771 496 3349
Fragments arranged by size:
3349 2771 2171 1214 635 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5371 1163
Size: 573 3635 6428
Fragments arranged by size:
6428 3635 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8086 8847 9875 1540
Size: 275 186 6085 761 1028 2301
Fragments arranged by size:
6085 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2408 3413 3577 3729 4440 4880 8697 8782 9853
Size: 1005 164 152 711 440 3817 85 1071
Cuts at: 9853 2408
Size: 3191
Fragments arranged by size:
3817 3191 1071 1005 711 440 164 152
85
BsmI GAATG_Cn'
Cuts at: 3618 5281 5911 8147 8240 3618
Size: 1663 630 2236 93 6014
Fragments arranged by size:
6014 2236 1663 630 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2408 3173 3413 3577 3729 3764
Size: 399 436 182 765 240 164 152 35
Cuts at: 3764 3777 3950 4440 4567 4880 5596 7955 8697
Size: 13 173 490 127 313 716 2359 742
Cuts at: 8697 8782 9853 1391
Size: 85 1071 2174
Fragments arranged by size:
2359 2174 1071 765 742 716 490 436
399 313 240 182 173 164 152 127
85 35 13
BspEI T'CCGG_A
Cuts at: 10299 10299
Size: 10636
BspHI T'CATG_A
Cuts at: 2748 5571 5934 8525 10255 2748
Size: 2823 363 2591 1730 3129
Fragments arranged by size:
3129 2823 2591 1730 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2323 5531 7991 844
Size: 1479 3208 2460 3489
Fragments arranged by size:
3489 3208 2460 1479
BsrBI CCG'CTC
Cuts at: 1394 1823 8523 1394
Size: 429 6700 3507
Fragments arranged by size:
6700 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 2082 2635 4044 6429 7236 7440
Size: 1183 768 66 553 1409 2385 807 204
Cuts at: 7440 9131 9305 65
Size: 1691 174 1396
Fragments arranged by size:
2385 1691 1409 1396 1183 807 768 553
204 174 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 3841 5331 9280 9467 1700
Size: 110 2031 1490 3949 187 2869
Fragments arranged by size:
3949 2869 2031 1490 187 110
BsrGI T'GTAC_A
Cuts at: 96 7233 96
Size: 7137 3499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10636
BssSI C'ACGA_G
Cuts at: 3028 8696 9708 3028
Size: 5668 1012 3956
Fragments arranged by size:
5668 3956 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10636
BstBI TT'CG_AA
Cuts at: 2644 2833 9440 2644
Size: 189 6607 3840
Fragments arranged by size:
6607 3840 189
BstEII G'GTnAC_C
Cuts at: 1184 4571 6155 6559 7163 7469 1184
Size: 3387 1584 404 604 306 4351
Fragments arranged by size:
4351 3387 1584 604 404 306
BstXI CCAn_nnnn'nTGG
Cuts at: 4110 4110
Size: 10636
BstYI r'GATC_y
Cuts at: 1972 2927 3698 4198 4210 4366 5056 5664 5815
Size: 955 771 500 12 156 690 608 151
Cuts at: 5815 6858 7399 7450 7476 7966 8333 8718 8735
Size: 1043 541 51 26 490 367 385 17
Cuts at: 8735 10187 10273 10285 10631 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1043 955 771 690 608 541
500 490 385 367 346 156 151 86
51 26 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2564 3210 4362 4688 7446 7458
Size: 552 939 560 646 1152 326 2758 12
Cuts at: 7458 513
Size: 3691
Fragments arranged by size:
3691 2758 1152 939 646 560 552 326
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6127 487
Size: 683 56 4901 4996
Fragments arranged by size:
4996 4901 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 4950 5299 5593 6222 8152 8946 8966 2161
Size: 2789 349 294 629 1930 794 20 3831
Fragments arranged by size:
3831 2789 1930 794 629 349 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10636
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4050 4085 529
Size: 35 3486 35 7080
Fragments arranged by size:
7080 3486 35 35
DraI TTT'AAA
Cuts at: 4239 7940 4239
Size: 3701 6935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4098 9643 809
Size: 507 2782 5545 1802
Fragments arranged by size:
5545 2782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4517 4590 6823
Size: 54 1425 50 275 18 2687 73 2233
Cuts at: 6823 8083 8972 10601 8
Size: 1260 889 1629 43
Fragments arranged by size:
2687 2233 1629 1425 1260 889 275 73
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8083 1537
Size: 275 6271 4090
Fragments arranged by size:
6271 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 3682 4077 6929 7413 7551 8566 1353
Size: 1293 1036 395 2852 484 138 1015 3423
Fragments arranged by size:
3423 2852 1293 1036 1015 484 395 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9223 9597 9743 387
Size: 1064 138 7634 374 146 1280
Fragments arranged by size:
7634 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3411 4438 4878 3411
Size: 1027 440 9169
Fragments arranged by size:
9169 1027 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2717 2837 3454 4919 5397 5669
Size: 393 617 468 120 617 1465 478 272
Cuts at: 5669 6624 6669 7271 7293 8699 9296 10083 10351
Size: 955 45 602 22 1406 597 787 268
Cuts at: 10351 1239
Size: 1524
Fragments arranged by size:
1524 1465 1406 955 787 617 617 602
597 478 468 393 272 268 120 45
22
EcoNI CCTnn'n_nnAGG
Cuts at: 2022 3067 3998 5379 6468 2022
Size: 1045 931 1381 1089 6190
Fragments arranged by size:
6190 1381 1089 1045 931
EcoO109I rG'GnC_Cy
Cuts at: 2027 3825 2027
Size: 1798 8838
EcoRI G'AATT_C
Cuts at: 2543 4283 5410 7945 8323 2543
Size: 1740 1127 2535 378 4856
Fragments arranged by size:
4856 2535 1740 1127 378
FspI TGC'GCA
Cuts at: 9142 9142
Size: 10636
HaeII r_GCGC'y
Cuts at: 1678 9783 1678
Size: 8105 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3488 3520 3683 3715 3890
Size: 32 135 32 1916 32 163 32 175
Cuts at: 3890 3922 5101 5133 5186 5218 7468 7500 7528
Size: 32 1179 32 53 32 2250 32 28
Cuts at: 7528 7560 7628 7660 9277 9309 9351 9383 1373
Size: 32 68 32 1617 32 42 32 2626
Fragments arranged by size:
2626 2250 1916 1617 1179 175 163 135
68 53 42 32 32 32 32 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 5476 6593 7980 8226 669
Size: 988 281 3538 1117 1387 246 3079
Fragments arranged by size:
3538 3079 1387 1117 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2735 5557 6368 748
Size: 1987 2822 811 5016
Fragments arranged by size:
5016 2822 1987 811
HpaI GTT'AAC
Cuts at: 5476 8226 5476
Size: 2750 7886
KpnI G_GTAC'C
Cuts at: 7961 7961
Size: 10636
MfeI C'AATT_G
Cuts at: 2703 3321 4905 5241 8235 2703
Size: 618 1584 336 2994 5104
Fragments arranged by size:
5104 2994 1584 618 336
MluI A'CGCG_T
Cuts at: 10512 10512
Size: 10636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6127 6987 7156 9750 9934 1305
Size: 4822 860 169 2594 184 2007
Fragments arranged by size:
4822 2594 2007 860 184 169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4519 4592 10
Size: 54 1425 343 2687 73 6054
Fragments arranged by size:
6054 2687 1425 343 73 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2429 2993 3552 4108 4583 5939
Size: 648 843 420 564 559 556 475 1356
Cuts at: 5939 6389 7445 8594 8953 9112 518
Size: 450 1056 1149 359 159 2042
Fragments arranged by size:
2042 1356 1149 1056 843 648 564 559
556 475 450 420 359 159
MspA1I CmG'CkG
Cuts at: 1606 2800 3048 3329 3335 4337 7391 8052 8729
Size: 1194 248 281 6 1002 3054 661 677
Cuts at: 8729 9877 10122 1606
Size: 1148 245 2120
Fragments arranged by size:
3054 2120 1194 1148 1002 677 661 281
248 245 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 3210 4362 7446 7458 513
Size: 552 939 1206 1152 3084 12 3691
Fragments arranged by size:
3691 3084 1206 1152 939 552 12
NdeI CA'TA_TG
Cuts at: 387 3217 4372 5713 6597 7843 387
Size: 2830 1155 1341 884 1246 3180
Fragments arranged by size:
3180 2830 1341 1246 1155 884
NheI G'CTAG_C
Cuts at: 1052 7081 1052
Size: 6029 4607
NotI GC'GGCC_GC
Cuts at: 8083 8083
Size: 10636
NspI r_CATG'y
Cuts at: 1132 1229 2395 4534 4627 6271 7006 7234 7994
Size: 97 1166 2139 93 1644 735 228 760
Cuts at: 7994 9539 1132
Size: 1545 2229
Fragments arranged by size:
2229 2139 1644 1545 1166 760 735 228
97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10636
PciI A'CATG_T
Cuts at: 6267 7002 7230 9535 6267
Size: 735 228 2305 7368
Fragments arranged by size:
7368 2305 735 228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5742 7175 1108
Size: 4634 1433 4569
Fragments arranged by size:
4634 4569 1433
PfoI T'CCnGG_A
Cuts at: 5646 7135 5646
Size: 1489 9147
PmeI GTTT'AAAC
Cuts at: 7940 7940
Size: 10636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8705 8737 10218 10250 8705
Size: 32 1481 32 9091
Fragments arranged by size:
9091 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 3825 2027
Size: 1798 8838
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10636
PsiI TTA'TAA
Cuts at: 5721 6635 8206 5721
Size: 914 1571 8151
Fragments arranged by size:
8151 1571 914
PstI C_TGCA'G
Cuts at: 830 7988 830
Size: 7158 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10636
PvuII CAG'CTG
Cuts at: 2800 3048 4337 7391 2800
Size: 248 1289 3054 6045
Fragments arranged by size:
6045 3054 1289 248
SacI G_AGCT'C
Cuts at: 3413 4440 4880 3413
Size: 1027 440 9169
Fragments arranged by size:
9169 1027 440
SalI G'TCGA_C
Cuts at: 1936 7978 1936
Size: 6042 4594
SapI GCTCTTCn'nnn_
Cuts at: 2646 6929 2646
Size: 4283 6353
SbfI CC_TGCA'GG
Cuts at: 7988 7988
Size: 10636
ScaI AGT'ACT
Cuts at: 7282 8884 10596 7282
Size: 1602 1712 7322
Fragments arranged by size:
7322 1712 1602
SexAI A'CCwGG_T
Cuts at: 4839 4839
Size: 10636
SfcI C'TryA_G
Cuts at: 826 1045 2795 4302 6696 7984 9119 9800 9991
Size: 219 1750 1507 2394 1288 1135 681 191
Cuts at: 9991 826
Size: 1471
Fragments arranged by size:
2394 1750 1507 1471 1288 1135 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10636
SmaI CCC'GGG
Cuts at: 1872 7963 1872
Size: 6091 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5129 7684 7792 8740 9641 9903
Size: 197 926 3186 2555 108 948 901 262
Cuts at: 9903 820
Size: 1553
Fragments arranged by size:
3186 2555 1553 948 926 901 262 197
108
SnaBI TAC'GTA
Cuts at: 493 8427 493
Size: 7934 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10636
SphI G_CATG'C
Cuts at: 1132 2395 4627 7994 1132
Size: 1263 2232 3367 3774
Fragments arranged by size:
3774 3367 2232 1263
SspI AAT'ATT
Cuts at: 5 52 2909 4399 6709 7182 8560 5
Size: 47 2857 1490 2310 473 1378 2081
Fragments arranged by size:
2857 2310 2081 1490 1378 473 47
StuI AGG'CCT
Cuts at: 1517 2694 5429 1517
Size: 1177 2735 6724
Fragments arranged by size:
6724 2735 1177
StyI C'CwwG_G
Cuts at: 513 1065 2004 2623 3210 3550 4106 4362 6484
Size: 552 939 619 587 340 556 256 2122
Cuts at: 6484 7446 7458 8075 513
Size: 962 12 617 3074
Fragments arranged by size:
3074 2122 962 939 619 617 587 556
552 340 256 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4284 7511 8663 8680 8833 9018 9463
Size: 1255 2704 3227 1152 17 153 185 445
Cuts at: 9463 325
Size: 1498
Fragments arranged by size:
3227 2704 1498 1255 1152 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2374 2472 4294
Size: 274 80 33 51 1443 397 98 1822
Cuts at: 4294 7233 7280 8882 10594 96
Size: 2939 47 1602 1712 138
Fragments arranged by size:
2939 1822 1712 1602 1443 397 274 138
98 80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4827 4859 5151 5183 6642 6674 7816 7848 10596
Size: 32 292 32 1459 32 1142 32 2748
Cuts at: 10596 10628 4827
Size: 32 4835
Fragments arranged by size:
4835 2748 1459 1142 292 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10636
XbaI T'CTAG_A
Cuts at: 5876 7972 5876
Size: 2096 8540
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10636
XmaI C'CCGG_G
Cuts at: 1870 7961 1870
Size: 6091 4545
XmnI GAAnn'nnTTC
Cuts at: 4706 4998 5694 6323 8765 4706
Size: 292 696 629 2442 6577
Fragments arranged by size:
6577 2442 696 629 292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8444 276
Size: 53 83 186 936 6910 2468
Fragments arranged by size:
6910 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI