(Circular) (Six-base) MAPSORT of: pFN21AE2318.seq Check: 3157 from: 1 to: 10645 pFN21AE2318 10645 bp With 182 enzymes: SgfI * June 13, 2011 13:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8455 278 Size: 53 83 186 936 6919 2468 Fragments arranged by size: 6919 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7988 1937 Size: 6051 4594 Acc65I G'GTAC_C Cuts at: 5290 7966 5290 Size: 2676 7969 AclI AA'CG_TT Cuts at: 1563 8772 9145 1563 Size: 7209 373 3063 Fragments arranged by size: 7209 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 2735 3348 3537 3685 4430 4927 5382 Size: 667 436 613 189 148 745 497 455 Cuts at: 5382 5421 7294 8708 10092 10360 1632 Size: 39 1873 1414 1384 268 1917 Fragments arranged by size: 1917 1873 1414 1384 745 667 613 497 455 436 268 189 148 39 AflII C'TTAA_G Cuts at: 820 1017 3320 5914 820 Size: 197 2303 2594 5551 Fragments arranged by size: 5551 2594 2303 197 AflIII A'CryG_T Cuts at: 1203 2493 4351 5253 6321 7056 7281 9544 10521 Size: 1290 1858 902 1068 735 225 2263 977 Cuts at: 10521 1203 Size: 1327 Fragments arranged by size: 2263 1858 1327 1290 1068 977 902 735 225 AgeI A'CCGG_T Cuts at: 5150 9476 5150 Size: 4326 6319 AhdI GACnn_n'nnGTC Cuts at: 5254 9374 5254 Size: 4120 6525 AleI CACnn'nnGTG Cuts at: 1166 5151 1166 Size: 3985 6660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10645 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 7604 7636 2480 Size: 32 5092 32 5489 Fragments arranged by size: 5489 5092 32 32 AlwNI CAG_nnn'CTG Cuts at: 2036 2951 3374 3643 8388 9960 2036 Size: 915 423 269 4745 1572 2721 Fragments arranged by size: 4745 2721 1572 915 423 269 ApaI G_GGCC'C Cuts at: 3826 3826 Size: 10645 ApaLI G'TGCA_C Cuts at: 2422 5048 8702 9858 2422 Size: 2626 3654 1156 3209 Fragments arranged by size: 3654 3209 2626 1156 ApoI r'AATT_y Cuts at: 1493 2385 2935 3296 3620 4178 4386 4612 7954 Size: 892 550 361 324 558 208 226 3342 Cuts at: 7954 8180 8332 10441 1493 Size: 226 152 2109 1697 Fragments arranged by size: 3342 2109 1697 892 558 550 361 324 226 226 208 152 AseI AT'TA_AT Cuts at: 160 1032 9199 160 Size: 872 8167 1606 Fragments arranged by size: 8167 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10645 AvaI C'yCGr_G Cuts at: 1870 1943 3539 3733 7970 1870 Size: 73 1596 194 4237 4545 Fragments arranged by size: 4545 4237 1596 194 73 AvrII C'CTAG_G Cuts at: 2641 2641 Size: 10645 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3004 3037 3004 Size: 33 10612 BamHI G'GATC_C Cuts at: 3535 4426 5356 5470 5559 7975 8342 3535 Size: 891 930 114 89 2416 367 5838 Fragments arranged by size: 5838 2416 930 891 367 114 89 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2729 2891 4622 5290 5642 Size: 325 217 627 942 162 1731 668 352 Cuts at: 5642 5873 5969 6902 7966 9421 618 Size: 231 96 933 1064 1455 1842 Fragments arranged by size: 1842 1731 1455 1064 942 933 668 627 352 325 231 217 162 96 BanII G_rGCy'C Cuts at: 1391 3422 3434 3740 3826 4940 6494 7491 7964 Size: 2031 12 306 86 1114 1554 997 473 Cuts at: 7964 1391 Size: 4072 Fragments arranged by size: 4072 2031 1554 1114 997 473 306 86 12 BbeI G_GCGC'C Cuts at: 5646 5973 6906 5646 Size: 327 933 9385 Fragments arranged by size: 9385 933 327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 4549 4654 5710 928 Size: 971 824 1826 105 1056 5863 Fragments arranged by size: 5863 1826 1056 971 824 105 BbvCI CC'TCA_GC Cuts at: 7114 7114 Size: 10645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4785 4819 7379 7413 8836 8870 1903 Size: 34 2848 34 2560 34 1423 34 3678 Fragments arranged by size: 3678 2848 2560 1423 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3005 6203 8537 9747 3005 Size: 3198 2334 1210 3903 Fragments arranged by size: 3903 3198 2334 1210 BclI T'GATC_A Cuts at: 1548 2412 6894 1548 Size: 864 4482 5299 Fragments arranged by size: 5299 4482 864 BfrBI ATG'CAT Cuts at: 4762 4762 Size: 10645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3086 9256 136 Size: 107 122 71 631 605 1414 6170 1525 Fragments arranged by size: 6170 1525 1414 631 605 122 107 71 BglII A'GATC_T Cuts at: 4998 7374 10640 4998 Size: 2376 3266 5003 Fragments arranged by size: 5003 3266 2376 BlpI GC'TnA_GC Cuts at: 3647 5633 8062 3647 Size: 1986 2429 6230 Fragments arranged by size: 6230 2429 1986 Bme1580I G_kGCm'C Cuts at: 1790 2426 2734 3826 4163 4627 5052 7470 8706 Size: 636 308 1092 337 464 425 2418 1236 Cuts at: 8706 9862 1790 Size: 1156 2573 Fragments arranged by size: 2573 2418 1236 1156 1092 636 464 425 337 308 BmgBI CAC'GTC Cuts at: 1318 4513 1318 Size: 3195 7450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7399 8376 9334 Size: 416 53 83 399 24 5982 977 958 Cuts at: 9334 442 Size: 1753 Fragments arranged by size: 5982 1753 977 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3290 4979 5759 7322 7799 9305 1239 Size: 1028 1023 1689 780 1563 477 1506 2579 Fragments arranged by size: 2579 1689 1563 1506 1028 1023 780 477 Bpu10I CC'TnA_GC Cuts at: 4934 7114 4934 Size: 2180 8465 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 5735 8770 9635 9933 2090 Size: 3645 3035 865 298 2802 Fragments arranged by size: 3645 3035 2802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3723 4814 5600 5902 7355 9308 882 Size: 617 2224 1091 786 302 1453 1953 2219 Fragments arranged by size: 2224 2219 1953 1453 1091 786 617 302 BsaAI yAC'GTr Cuts at: 493 1204 8436 493 Size: 711 7232 2702 Fragments arranged by size: 7232 2702 711 BsaBI GATnn'nnATC Cuts at: 5012 6254 6596 10639 5012 Size: 1242 342 4043 5018 Fragments arranged by size: 5018 4043 1242 342 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 5643 5970 6903 Size: 53 83 186 936 2455 1655 327 933 Cuts at: 6903 8452 8834 9469 275 Size: 1549 382 635 1451 Fragments arranged by size: 2455 1655 1549 1451 936 933 635 382 327 186 83 53 BsaWI w'CCGG_w Cuts at: 5150 5166 9078 9476 9750 9897 10308 5150 Size: 16 3912 398 274 147 411 5487 Fragments arranged by size: 5487 3912 411 398 274 147 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2480 2510 3722 3752 5378 Size: 30 165 30 1077 30 1212 30 1626 Cuts at: 5378 5408 1178 Size: 30 6415 Fragments arranged by size: 6415 1626 1212 1077 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3287 3539 3794 3871 5386 7703 1186 Size: 2101 252 255 77 1515 2317 4128 Fragments arranged by size: 4128 2317 2101 1515 255 252 77 BseYI C'CCAG_C Cuts at: 2993 3075 3896 6487 9848 10344 2993 Size: 82 821 2591 3361 496 3294 Fragments arranged by size: 3361 3294 2591 821 496 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4968 5175 7353 1163 Size: 573 3232 207 2178 4455 Fragments arranged by size: 4455 3232 2178 573 207 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8095 8856 9884 1540 Size: 275 186 6094 761 1028 2301 Fragments arranged by size: 6094 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2426 3434 3768 4326 4940 5052 5595 6717 7491 Size: 1008 334 558 614 112 543 1122 774 Cuts at: 7491 7817 8706 8791 9862 2426 Size: 326 889 85 1071 3209 Fragments arranged by size: 3209 1122 1071 1008 889 774 614 558 543 334 326 112 85 BsmI GAATG_Cn' Cuts at: 5962 8156 8249 5962 Size: 2194 93 8358 Fragments arranged by size: 8358 2194 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3995 3995 Size: 10645 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2426 2734 3422 3434 3740 3768 3826 Size: 399 636 308 688 12 306 28 58 Cuts at: 3826 4163 4326 4627 4940 5052 5595 6494 6717 Size: 337 163 301 313 112 543 899 223 Cuts at: 6717 7470 7491 7817 7964 8706 8791 9862 1391 Size: 753 21 326 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 753 742 688 636 543 399 337 326 313 308 306 301 223 163 147 112 85 58 28 21 12 BspEI T'CCGG_A Cuts at: 10308 10308 Size: 10645 BspHI T'CATG_A Cuts at: 2448 2610 3630 5622 5985 6444 6588 8534 10264 Size: 162 1020 1992 363 459 144 1946 1730 Cuts at: 10264 2448 Size: 2829 Fragments arranged by size: 2829 1992 1946 1730 1020 459 363 162 144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5582 8000 844 Size: 4738 2418 3489 Fragments arranged by size: 4738 3489 2418 BsrBI CCG'CTC Cuts at: 1394 1823 3879 7518 7618 8532 1394 Size: 429 2056 3639 100 914 3507 Fragments arranged by size: 3639 3507 2056 914 429 100 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 2535 2653 3005 3312 4323 5200 Size: 1183 840 447 118 352 307 1011 877 Cuts at: 5200 5295 7287 9140 9314 65 Size: 95 1992 1853 174 1396 Fragments arranged by size: 1992 1853 1396 1183 1011 877 840 447 352 307 174 118 95 BsrFI r'CCGG_y Cuts at: 1700 1810 5150 9289 9476 1700 Size: 110 3340 4139 187 2869 Fragments arranged by size: 4139 3340 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2340 6324 7284 96 Size: 2244 3984 960 3457 Fragments arranged by size: 3984 3457 2244 960 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10645 BssSI C'ACGA_G Cuts at: 7037 8705 9717 7037 Size: 1668 1012 7965 Fragments arranged by size: 7965 1668 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3347 7092 7436 7533 794 Size: 2553 3745 344 97 3906 Fragments arranged by size: 3906 3745 2553 344 97 BstBI TT'CG_AA Cuts at: 2836 9449 2836 Size: 6613 4032 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10645 BstXI CCAn_nnnn'nTGG Cuts at: 2589 5171 5189 5302 7300 2589 Size: 2582 18 113 1998 5934 Fragments arranged by size: 5934 2582 1998 113 18 BstYI r'GATC_y Cuts at: 1972 3535 4040 4426 4998 5356 5470 5559 7374 Size: 1563 505 386 572 358 114 89 1815 Cuts at: 7374 7501 7975 8342 8727 8744 10196 10282 10294 Size: 127 474 367 385 17 1452 86 12 Cuts at: 10294 10640 1972 Size: 346 1977 Fragments arranged by size: 1977 1815 1563 1452 572 505 474 386 385 367 358 346 127 114 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5457 5888 5457 Size: 431 10214 BtgI C'CryG_G Cuts at: 513 1065 2004 2582 3237 4056 4422 4674 513 Size: 552 939 578 655 819 366 252 6484 Fragments arranged by size: 6484 939 819 655 578 552 366 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3368 7477 487 Size: 683 56 2142 4109 3655 Fragments arranged by size: 4109 3655 2142 683 56 BtsI GCAGTG_nn' Cuts at: 3641 5084 5644 7531 7670 7707 8161 8955 8975 Size: 1443 560 1887 139 37 454 794 20 Cuts at: 8975 3641 Size: 5311 Fragments arranged by size: 5311 1887 1443 794 560 454 139 37 20 ClaI AT'CG_AT Cuts at: 1554 7050 1554 Size: 5496 5149 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3996 4031 6606 6641 529 Size: 35 3432 35 2575 35 4533 Fragments arranged by size: 4533 3432 2575 35 35 35 DraI TTT'AAA Cuts at: 3301 7949 3301 Size: 4648 5997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9652 809 Size: 507 8336 1802 Fragments arranged by size: 8336 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3234 3357 4566 Size: 54 1425 50 275 18 1404 123 1209 Cuts at: 4566 4650 8092 8981 10610 8 Size: 84 3442 889 1629 43 Fragments arranged by size: 3442 1629 1425 1404 1209 889 275 123 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8092 1537 Size: 275 6280 4090 Fragments arranged by size: 6280 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2318 2907 3330 3439 3488 3812 4137 Size: 833 132 589 423 109 49 324 325 Cuts at: 4137 5430 6983 7608 8575 1353 Size: 1293 1553 625 967 3423 Fragments arranged by size: 3423 1553 1293 967 833 625 589 423 325 324 132 109 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 9232 9606 9752 387 Size: 1064 138 2507 5136 374 146 1280 Fragments arranged by size: 5136 2507 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3432 4938 7489 3432 Size: 1506 2551 6588 Fragments arranged by size: 6588 2551 1506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2299 2735 3290 3348 3537 3685 Size: 393 635 32 436 555 58 189 148 Cuts at: 3685 4430 4927 4979 5382 5421 5759 7294 7322 Size: 745 497 52 403 39 338 1535 28 Cuts at: 7322 7799 8708 9305 10092 10360 1239 Size: 477 909 597 787 268 1524 Fragments arranged by size: 1535 1524 909 787 745 635 597 555 497 477 436 403 393 338 268 189 148 58 52 39 32 28 EcoNI CCTnn'n_nnAGG Cuts at: 3998 7175 3998 Size: 3177 7468 EcoO109I rG'GnC_Cy Cuts at: 2033 2578 3713 4034 5440 5892 7567 2033 Size: 545 1135 321 1406 452 1675 5111 Fragments arranged by size: 5111 1675 1406 1135 545 452 321 EcoRI G'AATT_C Cuts at: 7954 8332 7954 Size: 378 10267 EcoRV GAT'ATC Cuts at: 5419 5419 Size: 10645 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6205 6205 Size: 10645 FspI TGC'GCA Cuts at: 9151 9151 Size: 10645 HaeII r_GCGC'y Cuts at: 1678 3835 5646 5973 6906 9792 1678 Size: 2157 1811 327 933 2886 2531 Fragments arranged by size: 2886 2531 2157 1811 933 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3722 3754 4461 4493 5240 Size: 32 135 32 2150 32 707 32 747 Cuts at: 5240 5272 5378 5410 6622 6654 7507 7539 7679 Size: 32 106 32 1212 32 853 32 140 Cuts at: 7679 7711 9286 9318 9360 9392 1373 Size: 32 1575 32 42 32 2626 Fragments arranged by size: 2626 2150 1575 1212 853 747 707 140 135 106 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5527 6043 6115 7989 8235 669 Size: 988 281 3589 516 72 1874 246 3079 Fragments arranged by size: 3589 3079 1874 988 516 281 246 72 HindIII A'AGCT_T Cuts at: 748 6137 748 Size: 5389 5256 HpaI GTT'AAC Cuts at: 6043 8235 6043 Size: 2192 8453 KasI G'GCGC_C Cuts at: 5642 5969 6902 5642 Size: 327 933 9385 Fragments arranged by size: 9385 933 327 KpnI G_GTAC'C Cuts at: 5294 7970 5294 Size: 2676 7969 MfeI C'AATT_G Cuts at: 2721 3134 8244 2721 Size: 413 5110 5122 Fragments arranged by size: 5122 5110 413 MluI A'CGCG_T Cuts at: 10521 10521 Size: 10645 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3291 4433 5334 7569 9759 9943 1305 Size: 1986 1142 901 2235 2190 184 2007 Fragments arranged by size: 2235 2190 2007 1986 1142 901 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3236 3359 4568 4652 10 Size: 54 1425 343 1404 123 1209 84 6003 Fragments arranged by size: 6003 1425 1404 1209 343 123 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 3191 4421 4761 4841 5151 5169 Size: 648 1446 579 1230 340 80 310 18 Cuts at: 5169 5300 5621 5990 6443 7496 8603 8962 9121 Size: 131 321 369 453 1053 1107 359 159 Cuts at: 9121 518 Size: 2042 Fragments arranged by size: 2042 1446 1230 1107 1053 648 579 453 369 359 340 321 310 159 131 80 18 MspA1I CmG'CkG Cuts at: 1606 2818 3849 3858 4397 5208 5506 6269 7118 Size: 1212 1031 9 539 811 298 763 849 Cuts at: 7118 7882 8061 8738 9886 10131 1606 Size: 764 179 677 1148 245 2120 Fragments arranged by size: 2120 1212 1148 1031 849 811 764 763 677 539 298 245 179 9 NarI GG'CG_CC Cuts at: 5643 5970 6903 5643 Size: 327 933 9385 Fragments arranged by size: 9385 933 327 NcoI C'CATG_G Cuts at: 513 1065 2004 2582 3237 4056 4422 513 Size: 552 939 578 655 819 366 6736 Fragments arranged by size: 6736 939 819 655 578 552 366 NdeI CA'TA_TG Cuts at: 387 4758 7027 387 Size: 4371 2269 4005 Fragments arranged by size: 4371 4005 2269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10645 NotI GC'GGCC_GC Cuts at: 8092 8092 Size: 10645 NsiI A_TGCA'T Cuts at: 4764 4764 Size: 10645 NspI r_CATG'y Cuts at: 1132 1229 2959 4355 4594 5257 6325 7060 7285 Size: 97 1730 1396 239 663 1068 735 225 Cuts at: 7285 8003 9548 1132 Size: 718 1545 2229 Fragments arranged by size: 2229 1730 1545 1396 1068 735 718 663 239 225 97 PasI CC'CwG_GG Cuts at: 1348 4627 5854 7187 1348 Size: 3279 1227 1333 4806 Fragments arranged by size: 4806 3279 1333 1227 PciI A'CATG_T Cuts at: 4351 5253 6321 7056 7281 9544 4351 Size: 902 1068 735 225 2263 5452 Fragments arranged by size: 5452 2263 1068 902 735 225 PflMI CCAn_nnn'nTGG Cuts at: 1108 2230 4575 5793 6756 7193 7537 1108 Size: 1122 2345 1218 963 437 344 4216 Fragments arranged by size: 4216 2345 1218 1122 963 437 344 PfoI T'CCnGG_A Cuts at: 5697 5736 6278 7299 5697 Size: 39 542 1021 9043 Fragments arranged by size: 9043 1021 542 39 PmeI GTTT'AAAC Cuts at: 7949 7949 Size: 10645 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2512 2955 2987 3734 3766 8714 8746 10227 Size: 32 443 32 747 32 4948 32 1481 Cuts at: 10227 10259 2480 Size: 32 2866 Fragments arranged by size: 4948 2866 1481 747 443 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 3713 5440 5892 7567 2578 Size: 1135 1727 452 1675 5656 Fragments arranged by size: 5656 1727 1675 1135 452 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10645 PsiI TTA'TAA Cuts at: 8215 8215 Size: 10645 PspOMI G'GGCC_C Cuts at: 3822 3822 Size: 10645 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6650 6682 6650 Size: 32 10613 PstI C_TGCA'G Cuts at: 830 2817 7169 7702 7713 7997 830 Size: 1987 4352 533 11 284 3478 Fragments arranged by size: 4352 3478 1987 533 284 11 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10645 PvuII CAG'CTG Cuts at: 2818 6269 7118 2818 Size: 3451 849 6345 Fragments arranged by size: 6345 3451 849 SacI G_AGCT'C Cuts at: 3434 4940 7491 3434 Size: 1506 2551 6588 Fragments arranged by size: 6588 2551 1506 SalI G'TCGA_C Cuts at: 1936 7987 1936 Size: 6051 4594 SanDI GG'GwC_CC Cuts at: 2578 2578 Size: 10645 SapI GCTCTTCn'nnn_ Cuts at: 3439 6983 7608 3439 Size: 3544 625 6476 Fragments arranged by size: 6476 3544 625 SbfI CC_TGCA'GG Cuts at: 7997 7997 Size: 10645 ScaI AGT'ACT Cuts at: 6467 7418 8893 10605 6467 Size: 951 1475 1712 6507 Fragments arranged by size: 6507 1712 1475 951 SexAI A'CCwGG_T Cuts at: 6678 6678 Size: 10645 SfcI C'TryA_G Cuts at: 826 1045 2813 3844 3853 3862 4362 6270 7165 Size: 219 1768 1031 9 9 500 1908 895 Cuts at: 7165 7698 7709 7824 7993 9128 9809 10000 826 Size: 533 11 115 169 1135 681 191 1471 Fragments arranged by size: 1908 1768 1471 1135 1031 895 681 533 500 219 191 169 115 11 9 9 SfoI GGC'GCC Cuts at: 5644 5971 6904 5644 Size: 327 933 9385 Fragments arranged by size: 9385 933 327 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10645 SgrAI Cr'CCGG_yG Cuts at: 1700 5150 1700 Size: 3450 7195 SmaI CCC'GGG Cuts at: 1872 3735 7972 1872 Size: 1863 4237 4545 Fragments arranged by size: 4545 4237 1863 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 3320 5750 5914 8749 9650 Size: 197 926 162 1215 2430 164 2835 901 Cuts at: 9650 9912 820 Size: 262 1553 Fragments arranged by size: 2835 2430 1553 1215 926 901 262 197 164 162 SnaBI TAC'GTA Cuts at: 493 8436 493 Size: 7943 2702 SpeI A'CTAG_T Cuts at: 152 2524 4126 152 Size: 2372 1602 6671 Fragments arranged by size: 6671 2372 1602 SphI G_CATG'C Cuts at: 1132 2959 8003 1132 Size: 1827 5044 3774 Fragments arranged by size: 5044 3774 1827 SspI AAT'ATT Cuts at: 5 52 8569 5 Size: 47 8517 2081 Fragments arranged by size: 8517 2081 47 StuI AGG'CCT Cuts at: 1517 2229 2712 3587 1517 Size: 712 483 875 8575 Fragments arranged by size: 8575 875 712 483 StyI C'CwwG_G Cuts at: 513 1065 2004 2224 2582 2641 3237 4056 4422 Size: 552 939 220 358 59 596 819 366 Cuts at: 4422 5961 6538 7446 8084 513 Size: 1539 577 908 638 3074 Fragments arranged by size: 3074 1539 939 908 819 638 596 577 552 366 358 220 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 4610 6464 7200 7418 8672 8689 Size: 1255 1137 1893 1854 736 218 1254 17 Cuts at: 8689 8842 9027 9472 325 Size: 153 185 445 1498 Fragments arranged by size: 1893 1854 1498 1255 1254 1137 736 445 218 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 2490 5232 Size: 274 80 33 51 1443 363 150 2742 Cuts at: 5232 6324 6465 7284 7416 8891 10603 96 Size: 1092 141 819 132 1475 1712 138 Fragments arranged by size: 2742 1712 1475 1443 1092 819 363 274 150 141 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4602 4634 10605 10637 4602 Size: 32 5971 32 4610 Fragments arranged by size: 5971 4610 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5714 1316 Size: 4398 6247 XbaI T'CTAG_A Cuts at: 7981 7981 Size: 10645 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6484 7567 6484 Size: 1083 9562 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10645 XmaI C'CCGG_G Cuts at: 1870 3733 7970 1870 Size: 1863 4237 4545 Fragments arranged by size: 4545 4237 1863 XmnI GAAnn'nnTTC Cuts at: 3749 6377 6422 6764 8774 3749 Size: 2628 45 342 2010 5620 Fragments arranged by size: 5620 2628 2010 342 45 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8453 276 Size: 53 83 186 936 6919 2468 Fragments arranged by size: 6919 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BplI BsiWI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI RsrII SacII SfiI SrfI SwaI