Restriction map for
pFN21AE2313
(Circular) (Six-base) MAPSORT of: pFN21AE2313.seq Check: 3552 from: 1 to: 6580
pFN21AE2313 6580 bp
With 182 enzymes: SgfI *
January 12, 2011 17:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3633 3923 1937
Size: 1696 290 4594
Fragments arranged by size:
4594 1696 290
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 4643 6027 6295 1632
Size: 747 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 747 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2917 5479 6456 1203
Size: 1714 2562 977 1327
Fragments arranged by size:
2562 1714 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 4323 5895 4323
Size: 1572 5008
ApaLI G'TGCA_C
Cuts at: 4637 5793 4637
Size: 1156 5424
ApoI r'AATT_y
Cuts at: 1493 2229 2502 2867 3375 3889 4115 4267 6376
Size: 736 273 365 508 514 226 152 2109
Cuts at: 6376 1493
Size: 1697
Fragments arranged by size:
2109 1697 736 514 508 365 273 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2271 2633 3438 5134 160
Size: 872 1239 362 805 1696 1606
Fragments arranged by size:
1696 1606 1239 872 805 362
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 3905 1870
Size: 73 1962 4545
Fragments arranged by size:
4545 1962 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2303 2336 2303
Size: 33 6547
BamHI G'GATC_C
Cuts at: 3703 3910 4277 3703
Size: 207 367 6006
Fragments arranged by size:
6006 367 207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3901 5356 618
Size: 325 217 627 2114 1455 1842
Fragments arranged by size:
2114 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3899 1391
Size: 2508 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3165 928
Size: 971 1266 4343
Fragments arranged by size:
4343 1266 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3754 6575 3754
Size: 2821 3759
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 4641 5797 1790
Size: 2851 1156 2573
Fragments arranged by size:
2851 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4311 5269 442
Size: 416 53 83 399 24 2894 958 1753
Fragments arranged by size:
2894 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 2972 5240 1239
Size: 1236 497 2268 2579
Fragments arranged by size:
2579 2268 1236 497
Bpu10I CC'TnA_GC
Cuts at: 3232 3232
Size: 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 3553 3872 4705 5570 5868 2152
Size: 1401 319 833 865 298 2864
Fragments arranged by size:
2864 1401 865 833 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 5243 882
Size: 617 1684 2060 2219
Fragments arranged by size:
2219 2060 1684 617
BsaAI yAC'GTr
Cuts at: 493 1204 4371 493
Size: 711 3167 2702
Fragments arranged by size:
3167 2702 711
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4387 4769 5404 275
Size: 53 83 186 936 2854 382 635 1451
Fragments arranged by size:
2854 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5013 5411 5685 5832 6243 5013
Size: 398 274 147 411 5350
Fragments arranged by size:
5350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2568 2598 1178
Size: 30 165 30 1165 30 5160
Fragments arranged by size:
5160 1165 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 1186
Size: 1783 4797
BseYI C'CCAG_C
Cuts at: 5783 6279 5783
Size: 496 6084
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6007
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 4030 4791 5819 1540
Size: 275 186 1042 987 761 1028 2301
Fragments arranged by size:
2301 1042 1028 987 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4641 4726 5797 4641
Size: 85 1071 5424
Fragments arranged by size:
5424 1071 85
BsmI GAATG_Cn'
Cuts at: 4091 4184 4091
Size: 93 6487
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3899 4641 4726 5797 1391
Size: 399 2109 742 85 1071 2174
Fragments arranged by size:
2174 2109 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3935 844
Size: 3091 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4467 1394
Size: 429 2644 3507
Fragments arranged by size:
3507 2644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3819 5075 5249 65
Size: 1183 2571 1256 174 1396
Fragments arranged by size:
2571 1396 1256 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5224 5411 1700
Size: 110 3414 187 2869
Fragments arranged by size:
3414 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3355 96
Size: 3259 3321
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6580
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 2382 2382
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 2713 3703 3754 3910 4277 4662 4679 6131
Size: 741 990 51 156 367 385 17 1452
Cuts at: 6131 6217 6229 6575 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 990 741 385 367 346 156
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3453 513
Size: 552 2388 3640
Fragments arranged by size:
3640 2388 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 2174 4096 4890 4910 2174
Size: 1922 794 20 3844
Fragments arranged by size:
3844 1922 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3498 4027 4916
Size: 54 1425 50 275 18 1668 529 889
Cuts at: 4916 6545 8
Size: 1629 43
Fragments arranged by size:
1668 1629 1425 889 529 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3714 4510 1353
Size: 2361 796 3423
Fragments arranged by size:
3423 2361 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2475 2972 4643 5240 6027 6295
Size: 393 747 96 497 1671 597 787 268
Cuts at: 6295 1239
Size: 1524
Fragments arranged by size:
1671 1524 787 747 597 497 393 268
96
EcoNI CCTnn'n_nnAGG
Cuts at: 2721 2721
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 2726 2726
Size: 6580
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 5727 1678
Size: 4049 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2780 2812 2885 2917 3330
Size: 32 135 32 1208 32 73 32 413
Cuts at: 3330 3362 5221 5253 5295 5327 1373
Size: 32 1859 32 42 32 2626
Fragments arranged by size:
2626 1859 1208 413 135 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2128 3349 3924 4170 669
Size: 988 281 190 1221 575 246 3079
Fragments arranged by size:
3079 1221 988 575 281 246 190
HindIII A'AGCT_T
Cuts at: 748 2088 748
Size: 1340 5240
HpaI GTT'AAC
Cuts at: 2128 4170 2128
Size: 2042 4538
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5694 5878 1305
Size: 4389 184 2007
Fragments arranged by size:
4389 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3500 10
Size: 54 1425 343 1668 3090
Fragments arranged by size:
3090 1668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3222 4538 4897 5056 518
Size: 648 843 1213 1316 359 159 2042
Fragments arranged by size:
2042 1316 1213 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2357 3996 4673 5821 6066 1606
Size: 751 1639 677 1148 245 2120
Fragments arranged by size:
2120 1639 1148 751 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3453 513
Size: 552 2388 3640
Fragments arranged by size:
3640 2388 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 2063 2921 3938 5483 1132
Size: 97 834 858 1017 1545 2229
Fragments arranged by size:
2229 1545 1017 858 834 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 2917 5479 2917
Size: 2562 4018
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6580
PfoI T'CCnGG_A
Cuts at: 3726 3726
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2726 2726
Size: 6580
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PstI C_TGCA'G
Cuts at: 830 2268 2418 3932 830
Size: 1438 150 1514 3478
Fragments arranged by size:
3478 1514 1438 150
PvuI CG_AT'CG
Cuts at: 2001 3043 2001
Size: 1042 5538
PvuII CAG'CTG
Cuts at: 2357 2357
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 2622 4828 6540 2622
Size: 2206 1712 2662
Fragments arranged by size:
2662 2206 1712
SfcI C'TryA_G
Cuts at: 826 1045 2264 2414 3928 5063 5744 5935 826
Size: 219 1219 150 1514 1135 681 191 1471
Fragments arranged by size:
1514 1471 1219 1135 681 219 191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2131 3568 3851 4684 5585 5847
Size: 197 926 188 1437 283 833 901 262
Cuts at: 5847 820
Size: 1553
Fragments arranged by size:
1553 1437 926 901 833 283 262 197
188
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 2015 152
Size: 1863 4717
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 3330 4504 5
Size: 47 3278 1174 2081
Fragments arranged by size:
3278 2081 1174 47
StuI AGG'CCT
Cuts at: 1517 2960 1517
Size: 1443 5137
StyI C'CwwG_G
Cuts at: 513 1065 2277 2381 3453 4019 513
Size: 552 1212 104 1072 566 3074
Fragments arranged by size:
3074 1212 1072 566 552 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4607 4624 4777 4962 5407 325
Size: 1255 3027 17 153 185 445 1498
Fragments arranged by size:
3027 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 3355 4826
Size: 274 80 33 51 1443 643 735 1471
Cuts at: 4826 6538 96
Size: 1712 138
Fragments arranged by size:
1712 1471 1443 735 643 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6540 6572 6540
Size: 32 6548
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 2710 3625 3916 2710
Size: 915 291 5374
Fragments arranged by size:
5374 915 291
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 2688 4709 2688
Size: 2021 4559
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI