Restriction map for
pFN21AE2310
(Circular) (Six-base) MAPSORT of: pFN21AE2310.seq Check: 3321 from: 1 to: 8629
pFN21AE2310 8629 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6439 278
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2805 4678 4894 5972 1937
Size: 514 354 1873 216 1078 4594
Fragments arranged by size:
4594 1873 1078 514 354 216
Acc65I G'GTAC_C
Cuts at: 4216 5950 4216
Size: 1734 6895
AclI AA'CG_TT
Cuts at: 1563 4816 6756 7129 1563
Size: 3253 1940 373 3063
Fragments arranged by size:
3253 3063 1940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3229 3374 3421 3519 3565 4178 4242 5690
Size: 1597 145 47 98 46 613 64 1448
Cuts at: 5690 5747 5894 6692 8076 8344 1632
Size: 57 147 798 1384 268 1917
Fragments arranged by size:
1917 1597 1448 1384 798 613 268 147
145 98 64 57 47 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8432
AflIII A'CryG_T
Cuts at: 1203 2262 7528 8505 1203
Size: 1059 5266 977 1327
Fragments arranged by size:
5266 1327 1059 977
AgeI A'CCGG_T
Cuts at: 7460 7460
Size: 8629
AhdI GACnn_n'nnGTC
Cuts at: 2027 2678 4275 7358 2027
Size: 651 1597 3083 3298
Fragments arranged by size:
3298 3083 1597 651
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5906 1184
Size: 4722 3907
AlwNI CAG_nnn'CTG
Cuts at: 2688 3352 5885 6372 7944 2688
Size: 664 2533 487 1572 3373
Fragments arranged by size:
3373 2533 1572 664 487
ApaLI G'TGCA_C
Cuts at: 6686 7842 6686
Size: 1156 7473
ApoI r'AATT_y
Cuts at: 1493 2305 2462 2755 3068 3097 3629 4691 4773
Size: 812 157 293 313 29 532 1062 82
Cuts at: 4773 4901 5938 6164 6316 8425 1493
Size: 128 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 1037 812 532 313 293
226 157 152 128 82 29
AseI AT'TA_AT
Cuts at: 160 1032 7183 160
Size: 872 6151 1606
Fragments arranged by size:
6151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
AvaI C'yCGr_G
Cuts at: 1870 1943 4938 5954 1870
Size: 73 2995 1016 4545
Fragments arranged by size:
4545 2995 1016 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2123 2156 2123
Size: 33 8596
BamHI G'GATC_C
Cuts at: 5823 5959 6326 5823
Size: 136 367 8126
Fragments arranged by size:
8126 367 136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4216 5330 5350 5950 7405
Size: 325 217 627 2429 1114 20 600 1455
Cuts at: 7405 618
Size: 1842
Fragments arranged by size:
2429 1842 1455 1114 627 600 325 217
20
BanII G_rGCy'C
Cuts at: 1391 2117 3987 4373 5254 5426 5948 1391
Size: 726 1870 386 881 172 522 4072
Fragments arranged by size:
4072 1870 881 726 522 386 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7658
BbvCI CC'TCA_GC
Cuts at: 4824 5061 4824
Size: 237 8392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6820 6854 1903
Size: 34 4883 34 3678
Fragments arranged by size:
4883 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6521 7731 6521
Size: 1210 7419
BclI T'GATC_A
Cuts at: 1548 4314 1548
Size: 2766 5863
BfrBI ATG'CAT
Cuts at: 5576 5576
Size: 8629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7240 136
Size: 107 122 71 631 605 5568 1525
Fragments arranged by size:
5568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8624 8624
Size: 8629
BlpI GC'TnA_GC
Cuts at: 6046 6046
Size: 8629
Bme1580I G_kGCm'C
Cuts at: 1790 5244 6690 7846 1790
Size: 3454 1446 1156 2573
Fragments arranged by size:
3454 2573 1446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3670 4098 4986
Size: 416 53 83 399 24 2253 428 888
Cuts at: 4986 6360 7318 442
Size: 1374 958 1753
Fragments arranged by size:
2253 1753 1374 958 888 428 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4598 1056
Size: 3542 5087
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 8629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2321 2730 2849 3990 4691 7289 1239
Size: 974 108 409 119 1141 701 2598 2579
Fragments arranged by size:
2598 2579 1141 974 701 409 119 108
Bpu10I CC'TnA_GC
Cuts at: 2719 4180 4488 4824 5061 2719
Size: 1461 308 336 237 6287
Fragments arranged by size:
6287 1461 336 308 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 4081 6754 7619 7917 3299
Size: 782 2673 865 298 4011
Fragments arranged by size:
4011 2673 865 782 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4935 5532 7292 882
Size: 617 3436 597 1760 2219
Fragments arranged by size:
3436 2219 1760 617 597
BsaAI yAC'GTr
Cuts at: 493 1204 2263 6420 493
Size: 711 1059 4157 2702
Fragments arranged by size:
4157 2702 1059 711
BsaBI GATnn'nnATC
Cuts at: 3834 8623 3834
Size: 4789 3840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6436 6818 7453 275
Size: 53 83 186 936 4903 382 635 1451
Fragments arranged by size:
4903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 5913 7062 7460 7734 7881 8292 4347
Size: 1566 1149 398 274 147 411 4684
Fragments arranged by size:
4684 1566 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 2763 2793 4218
Size: 30 165 30 1030 30 300 30 1425
Cuts at: 4218 4248 5223 5253 1178
Size: 30 975 30 4554
Fragments arranged by size:
4554 1425 1030 975 300 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3692 5078 5151 5445 5519 1186
Size: 2506 1386 73 294 74 4296
Fragments arranged by size:
4296 2506 1386 294 74 73
BseYI C'CCAG_C
Cuts at: 2069 2595 4354 7832 8328 2069
Size: 526 1759 3478 496 2370
Fragments arranged by size:
3478 2370 1759 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2664 3850 4822 1163
Size: 573 376 552 1186 972 4970
Fragments arranged by size:
4970 1186 972 573 552 376
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6079 6840 7868 1540
Size: 275 186 4078 761 1028 2301
Fragments arranged by size:
4078 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2214 2835 3746 4437 5759 6690 6775 7846
Size: 97 621 911 691 1322 931 85 1071
Cuts at: 7846 2117
Size: 2900
Fragments arranged by size:
2900 1322 1071 931 911 691 621 97
85
BsmI GAATG_Cn'
Cuts at: 2864 6140 6233 2864
Size: 3276 93 5260
Fragments arranged by size:
5260 3276 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2214 2835 3746 3987 4373 4437
Size: 399 327 97 621 911 241 386 64
Cuts at: 4437 5244 5254 5426 5759 5948 6690 6775 7846
Size: 807 10 172 333 189 742 85 1071
Cuts at: 7846 1391
Size: 2174
Fragments arranged by size:
2174 1071 911 807 742 621 399 386
333 327 241 189 172 97 85 64
10
BspEI T'CCGG_A
Cuts at: 8292 8292
Size: 8629
BspHI T'CATG_A
Cuts at: 3895 6518 8248 3895
Size: 2623 1730 4276
Fragments arranged by size:
4276 2623 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 5787 5984 844
Size: 1457 3486 197 3489
Fragments arranged by size:
3489 3486 1457 197
BsrBI CCG'CTC
Cuts at: 1394 1823 6516 1394
Size: 429 4693 3507
Fragments arranged by size:
4693 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 7124 7298 65
Size: 1183 1366 4510 174 1396
Fragments arranged by size:
4510 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 7273 7460 1700
Size: 110 201 5262 187 2869
Fragments arranged by size:
5262 2869 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 3734 96
Size: 3638 4991
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8629
BssSI C'ACGA_G
Cuts at: 2045 4430 5427 6689 7701 2045
Size: 2385 997 1262 1012 2973
Fragments arranged by size:
2973 2385 1262 1012 997
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5291 794
Size: 4497 4132
BstBI TT'CG_AA
Cuts at: 7433 7433
Size: 8629
BstEII G'GTnAC_C
Cuts at: 1184 3265 1184
Size: 2081 6548
BstXI CCAn_nnnn'nTGG
Cuts at: 3661 4384 4606 4659 5650 3661
Size: 723 222 53 991 6640
Fragments arranged by size:
6640 991 723 222 53
BstYI r'GATC_y
Cuts at: 1972 3594 5823 5959 6326 6711 6728 8180 8266
Size: 1622 2229 136 367 385 17 1452 86
Cuts at: 8266 8278 8624 1972
Size: 12 346 1977
Fragments arranged by size:
2229 1977 1622 1452 385 367 346 136
86 17 12
BstZ17I GTA'TAC
Cuts at: 4679 4679
Size: 8629
Bsu36I CC'TnA_GG
Cuts at: 2727 5079 2727
Size: 2352 6277
BtgI C'CryG_G
Cuts at: 513 1065 2513 3712 3914 4405 513
Size: 552 1448 1199 202 491 4737
Fragments arranged by size:
4737 1448 1199 552 491 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7890
Fragments arranged by size:
7890 683 56
BtsI GCAGTG_nn'
Cuts at: 6145 6939 6959 6145
Size: 794 20 7815
Fragments arranged by size:
7815 794 20
ClaI AT'CG_AT
Cuts at: 1554 2344 1554
Size: 790 7839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2749 2784 3753 3788 5389 5424 529
Size: 35 2185 35 969 35 1601 35 3734
Fragments arranged by size:
3734 2185 1601 969 35 35 35 35
DraI TTT'AAA
Cuts at: 2367 3754 5933 2367
Size: 1387 2179 5063
Fragments arranged by size:
5063 2179 1387
DraIII CAC_nnn'GTG
Cuts at: 5291 5291
Size: 8629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2536 7636 809
Size: 507 1220 5100 1802
Fragments arranged by size:
5100 1802 1220 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2587 3224 6076
Size: 54 1425 50 275 18 757 637 2852
Cuts at: 6076 6965 8594 8
Size: 889 1629 43
Fragments arranged by size:
2852 1629 1425 889 757 637 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6076 1537
Size: 275 4264 4090
Fragments arranged by size:
4264 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 3440 3992 4031 4154 5028 6559 1353
Size: 1921 166 552 39 123 874 1531 3423
Fragments arranged by size:
3423 1921 1531 874 552 166 123 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5836 7216 7590 7736 387
Size: 1064 138 4247 1380 374 146 1280
Fragments arranged by size:
4247 1380 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2115 2115
Size: 8629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2321 2730 2849 3229 3374 3421
Size: 393 581 108 409 119 380 145 47
Cuts at: 3421 3519 3565 3990 4178 4242 4691 5690 5747
Size: 98 46 425 188 64 449 999 57
Cuts at: 5747 5894 6692 7289 8076 8344 1239
Size: 147 798 597 787 268 1524
Fragments arranged by size:
1524 999 798 787 597 581 449 425
409 393 380 268 188 147 145 119
108 98 64 57 47 46
EcoO109I rG'GnC_Cy
Cuts at: 2221 2600 2723 3130 3545 3722 2221
Size: 379 123 407 415 177 7128
Fragments arranged by size:
7128 415 407 379 177 123
EcoRI G'AATT_C
Cuts at: 4773 5938 6316 4773
Size: 1165 378 7086
Fragments arranged by size:
7086 1165 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4148 4148
Size: 8629
FspI TGC'GCA
Cuts at: 2153 7135 2153
Size: 4982 3647
HaeII r_GCGC'y
Cuts at: 1678 7776 1678
Size: 6098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2066 2098 2664
Size: 32 135 32 441 32 21 32 566
Cuts at: 2664 2696 3022 3054 3579 3611 4261 4293 7270
Size: 32 326 32 525 32 650 32 2977
Cuts at: 7270 7302 7344 7376 1373
Size: 32 42 32 2626
Fragments arranged by size:
2977 2626 650 566 525 441 326 135
42 32 32 32 32 32 32 32
32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 5973 6219 669
Size: 988 281 603 3432 246 3079
Fragments arranged by size:
3432 3079 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 3000 4985 748
Size: 2252 1985 4392
Fragments arranged by size:
4392 2252 1985
HpaI GTT'AAC
Cuts at: 6219 6219
Size: 8629
KpnI G_GTAC'C
Cuts at: 4220 5954 4220
Size: 1734 6895
MfeI C'AATT_G
Cuts at: 3144 6228 3144
Size: 3084 5545
MluI A'CGCG_T
Cuts at: 8505 8505
Size: 8629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 2837 4398 4802 4853 7743 7927 1305
Size: 1242 290 1561 404 51 2890 184 2007
Fragments arranged by size:
2890 2007 1561 1242 404 290 184 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3226 10
Size: 54 1425 343 1394 5413
Fragments arranged by size:
5413 1425 1394 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3659 4704 5177 6587 6946 7105 518
Size: 648 2493 1045 473 1410 359 159 2042
Fragments arranged by size:
2493 2042 1410 1045 648 473 359 159
MspA1I CmG'CkG
Cuts at: 1606 3968 4384 6045 6722 7870 8115 1606
Size: 2362 416 1661 677 1148 245 2120
Fragments arranged by size:
2362 2120 1661 1148 677 416 245
NaeI GCC'GGC
Cuts at: 2013 2013
Size: 8629
NcoI C'CATG_G
Cuts at: 513 1065 2513 3712 3914 4405 513
Size: 552 1448 1199 202 491 4737
Fragments arranged by size:
4737 1448 1199 552 491 202
NdeI CA'TA_TG
Cuts at: 387 2857 387
Size: 2470 6159
NgoMIV G'CCGG_C
Cuts at: 2011 2011
Size: 8629
NheI G'CTAG_C
Cuts at: 1052 4594 1052
Size: 3542 5087
NotI GC'GGCC_GC
Cuts at: 6076 6076
Size: 8629
NsiI A_TGCA'T
Cuts at: 5578 5578
Size: 8629
NspI r_CATG'y
Cuts at: 1132 1229 2778 3711 3947 5987 7532 1132
Size: 97 1549 933 236 2040 1545 2229
Fragments arranged by size:
2229 2040 1549 1545 933 236 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8629
PciI A'CATG_T
Cuts at: 7528 7528
Size: 8629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 3423 1108
Size: 1382 933 6314
Fragments arranged by size:
6314 1382 933
PfoI T'CCnGG_A
Cuts at: 3465 3465
Size: 8629
PmeI GTTT'AAAC
Cuts at: 5933 5933
Size: 8629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6698 6730 8211 8243 6698
Size: 32 1481 32 7084
Fragments arranged by size:
7084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 2600 2723 3130 3545 3722 2221
Size: 379 123 407 415 177 7128
Fragments arranged by size:
7128 415 407 379 177 123
PshAI GACnn'nnGTC
Cuts at: 1613 3561 1613
Size: 1948 6681
PsiI TTA'TAA
Cuts at: 4203 5808 6199 4203
Size: 1605 391 6633
Fragments arranged by size:
6633 1605 391
PstI C_TGCA'G
Cuts at: 830 5784 5981 830
Size: 4954 197 3478
Fragments arranged by size:
4954 3478 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8629
PvuII CAG'CTG
Cuts at: 3968 4384 3968
Size: 416 8213
SacI G_AGCT'C
Cuts at: 2117 2117
Size: 8629
SalI G'TCGA_C
Cuts at: 1936 5971 1936
Size: 4035 4594
SanDI GG'GwC_CC
Cuts at: 2600 3130 2600
Size: 530 8099
SapI GCTCTTCn'nnn_
Cuts at: 3992 4031 3992
Size: 39 8590
SbfI CC_TGCA'GG
Cuts at: 5784 5981 5784
Size: 197 8432
ScaI AGT'ACT
Cuts at: 2159 3285 5069 6877 8589 2159
Size: 1126 1784 1808 1712 2199
Fragments arranged by size:
2199 1808 1784 1712 1126
SexAI A'CCwGG_T
Cuts at: 3261 3261
Size: 8629
SfcI C'TryA_G
Cuts at: 826 1045 2371 3289 3338 5245 5780 5977 7112
Size: 219 1326 918 49 1907 535 197 1135
Cuts at: 7112 7793 7984 826
Size: 681 191 1471
Fragments arranged by size:
1907 1471 1326 1135 918 681 535 219
197 191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8629
SmaI CCC'GGG
Cuts at: 1872 5956 1872
Size: 4084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3314 4060 4938 6733 7634 7896
Size: 197 926 1371 746 878 1795 901 262
Cuts at: 7896 820
Size: 1553
Fragments arranged by size:
1795 1553 1371 926 901 878 746 262
197
SnaBI TAC'GTA
Cuts at: 493 6420 493
Size: 5927 2702
SpeI A'CTAG_T
Cuts at: 152 3890 152
Size: 3738 4891
SphI G_CATG'C
Cuts at: 1132 5987 1132
Size: 4855 3774
SspI AAT'ATT
Cuts at: 5 52 2361 5801 6553 5
Size: 47 2309 3440 752 2081
Fragments arranged by size:
3440 2309 2081 752 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8629
StyI C'CwwG_G
Cuts at: 513 1065 2352 2513 3712 3871 3914 4174 4281
Size: 552 1287 161 1199 159 43 260 107
Cuts at: 4281 4405 5592 5649 6068 513
Size: 124 1187 57 419 3074
Fragments arranged by size:
3074 1287 1199 1187 552 419 260 161
159 124 107 57 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 3864 3884 4132 4346 4712 5393
Size: 1255 443 1841 20 248 214 366 681
Cuts at: 5393 5605 5784 6656 6673 6826 7011 7456 325
Size: 212 179 872 17 153 185 445 1498
Fragments arranged by size:
1841 1498 1255 872 681 445 443 366
248 214 212 185 179 153 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2582 2850
Size: 274 80 33 51 1443 180 425 268
Cuts at: 2850 3283 3491 3734 4626 5067 5787 6875 8587
Size: 433 208 243 892 441 720 1088 1712
Cuts at: 8587 96
Size: 138
Fragments arranged by size:
1712 1443 1088 892 720 441 433 425
274 268 243 208 180 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 4218 4250 4329 4361 5223 5255 5343
Size: 32 1560 32 79 32 862 32 88
Cuts at: 5343 5375 5561 5593 8589 8621 2626
Size: 32 186 32 2996 32 2634
Fragments arranged by size:
2996 2634 1560 862 186 88 79 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3720 1316
Size: 2404 6225
XbaI T'CTAG_A
Cuts at: 5965 5965
Size: 8629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2824 4288 2824
Size: 1464 7165
XhoI C'TCGA_G
Cuts at: 1943 4938 1943
Size: 2995 5634
XmaI C'CCGG_G
Cuts at: 1870 5954 1870
Size: 4084 4545
XmnI GAAnn'nnTTC
Cuts at: 2462 6758 2462
Size: 4296 4333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6437 276
Size: 53 83 186 936 4903 2468
Fragments arranged by size:
4903 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI