Restriction map for
pFN21AE2307
(Circular) (Six-base) MAPSORT of: pFN21AE2307.seq Check: 888 from: 1 to: 5611
pFN21AE2307 5611 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2431 2954 1937
Size: 494 523 4594
Fragments arranged by size:
4594 523 494
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2762 3674 5058 5326 1632
Size: 1130 912 1384 268 1917
Fragments arranged by size:
1917 1384 1130 912 268
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 5611
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2614 4510 5487 1203
Size: 1411 1896 977 1327
Fragments arranged by size:
1896 1411 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2591 3354 4926 2591
Size: 763 1572 3276
Fragments arranged by size:
3276 1572 763
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2285 2920 3146 3298 5407 1493
Size: 792 635 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2936 1870
Size: 73 993 4545
Fragments arranged by size:
4545 993 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2367 2932 4387 618
Size: 325 217 627 580 565 1455 1842
Fragments arranged by size:
1842 1455 627 580 565 325 217
BanII G_rGCy'C
Cuts at: 1391 2692 2930 1391
Size: 1301 238 4072
Fragments arranged by size:
4072 1301 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BbvCI CC'TCA_GC
Cuts at: 2731 2731
Size: 5611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3802 3836 1903
Size: 34 50 34 1781 34 3678
Fragments arranged by size:
3678 1781 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2823 3503 4713 2823
Size: 680 1210 3721
Fragments arranged by size:
3721 1210 680
BclI T'GATC_A
Cuts at: 1548 2353 1548
Size: 805 4806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2447 5606 2447
Size: 3159 2452
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 3672 4828 1790
Size: 1882 1156 2573
Fragments arranged by size:
2573 1882 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3342 4300
Size: 416 53 83 399 24 874 1051 958
Cuts at: 4300 442
Size: 1753
Fragments arranged by size:
1753 1051 958 874 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4271 1239
Size: 3032 2579
Bpu10I CC'TnA_GC
Cuts at: 2731 2731
Size: 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2512 3736 4601 4899 2512
Size: 1224 865 298 3224
Fragments arranged by size:
3224 1224 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2494 2524 1178
Size: 30 165 30 1091 30 4265
Fragments arranged by size:
4265 1091 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2515 1186
Size: 1329 4282
BseYI C'CCAG_C
Cuts at: 2685 4814 5310 2685
Size: 2129 496 2986
Fragments arranged by size:
2986 2129 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5038
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3672 3757 4828 3672
Size: 85 1071 4455
Fragments arranged by size:
4455 1071 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2692 2930 3672 3757 4828 1391
Size: 399 902 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 902 742 399 238 85
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 844
Size: 2122 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4255 4442 1700
Size: 110 2445 187 2869
Fragments arranged by size:
2869 2445 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 2046 3671 4683 2046
Size: 1625 1012 2974
Fragments arranged by size:
2974 1625 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 2653
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2447 2941 3308 3693 3710 5162 5248 5260
Size: 475 494 367 385 17 1452 86 12
Cuts at: 5260 5606 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 494 475 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5059
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3921 3941 3127
Size: 794 20 4797
Fragments arranged by size:
4797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2392 2427 529
Size: 35 1828 35 3713
Fragments arranged by size:
3713 1828 35 35
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2655 3058
Size: 54 1425 50 275 18 345 480 403
Cuts at: 3058 3947 5576 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 480 403 345 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2604 3541 1353
Size: 1051 200 937 3423
Fragments arranged by size:
3423 1051 937 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 3674 4271 5058 5326 1239
Size: 393 1130 912 597 787 268 1524
Fragments arranged by size:
1524 1130 912 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2741 2741
Size: 5611
EcoRI G'AATT_C
Cuts at: 2285 2920 3298 2285
Size: 635 378 4598
Fragments arranged by size:
4598 635 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 5611
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2833 4758 1678
Size: 1155 1925 2531
Fragments arranged by size:
2531 1925 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373
Size: 32 135 32 2680 32 42 32 2626
Fragments arranged by size:
2680 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 2553 2709 3210 2553
Size: 156 501 4954
Fragments arranged by size:
4954 501 156
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2427 4725 4909 1305
Size: 1122 2298 184 2007
Fragments arranged by size:
2298 2007 1122 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 2657 10
Size: 54 1425 343 345 480 2964
Fragments arranged by size:
2964 1425 480 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3569 3928 4087 518
Size: 648 2403 359 159 2042
Fragments arranged by size:
2403 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3027 3704 4852 5097 1606
Size: 1421 677 1148 245 2120
Fragments arranged by size:
2120 1421 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5059
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2618 2969 4514 1132
Size: 97 1389 351 1545 2229
Fragments arranged by size:
2229 1545 1389 351 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 2614 4510 2614
Size: 1896 3715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PfoI T'CCnGG_A
Cuts at: 2380 2380
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2741 2741
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 2393 1613
Size: 780 4831
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2963 830
Size: 2133 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2604 2604
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2592 2959 4094 4775 4966 826
Size: 219 1547 367 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2527 3715 4616 4878 820
Size: 197 926 584 1188 901 262 1553
Fragments arranged by size:
1553 1188 926 901 584 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 2329 2856 3535 5
Size: 47 2277 527 679 2081
Fragments arranged by size:
2277 2081 679 527 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 3050 513
Size: 552 1985 3074
Fragments arranged by size:
3074 1985 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2886 3638 3655 3808 3993 4438 325
Size: 1255 1306 752 17 153 185 445 1498
Fragments arranged by size:
1498 1306 1255 752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 2378
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI