Restriction map for
pFN21AE2302
(Circular) (Six-base) MAPSORT of: pFN21AE2302.seq Check: 8520 from: 1 to: 11227
pFN21AE2302 11227 bp
With 182 enzymes: SgfI *
November 26, 2010 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6922 6922
Size: 11227
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9037 278
Size: 53 83 186 936 7501 2468
Fragments arranged by size:
7501 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 6722 7522 8570 1937
Size: 625 4160 800 1048 4594
Fragments arranged by size:
4594 4160 1048 800 625
Acc65I G'GTAC_C
Cuts at: 8548 8548
Size: 11227
AclI AA'CG_TT
Cuts at: 1563 5488 9354 9727 1563
Size: 3925 3866 373 3063
Fragments arranged by size:
3925 3866 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 3036 4845 5223 5365 5843 5855 6540
Size: 1143 261 1809 378 142 478 12 685
Cuts at: 6540 6561 7905 8165 8304 8367 9290 10674 10942
Size: 21 1344 260 139 63 923 1384 268
Cuts at: 10942 1632
Size: 1917
Fragments arranged by size:
1917 1809 1384 1344 1143 923 685 478
378 268 261 260 142 139 63 21
12
AflII C'TTAA_G
Cuts at: 820 1017 2186 8291 820
Size: 197 1169 6105 3756
Fragments arranged by size:
6105 3756 1169 197
AflIII A'CryG_T
Cuts at: 1203 5521 5668 5739 7686 10126 11103 1203
Size: 4318 147 71 1947 2440 977 1327
Fragments arranged by size:
4318 2440 1947 1327 977 147 71
AgeI A'CCGG_T
Cuts at: 10058 10058
Size: 11227
AhdI GACnn_n'nnGTC
Cuts at: 3748 9956 3748
Size: 6208 5019
AleI CACnn'nnGTG
Cuts at: 1166 3093 3438 3624 1166
Size: 1927 345 186 8769
Fragments arranged by size:
8769 1927 345 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6385 1184
Size: 5201 6026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4590 4622 7486 7518 4590
Size: 32 2864 32 8299
Fragments arranged by size:
8299 2864 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3059 7087 7790 8970 10542 3059
Size: 4028 703 1180 1572 3744
Fragments arranged by size:
4028 3744 1572 1180 703
ApaLI G'TGCA_C
Cuts at: 4974 5590 9284 10440 4974
Size: 616 3694 1156 5761
Fragments arranged by size:
5761 3694 1156 616
ApoI r'AATT_y
Cuts at: 1493 2407 3044 3589 3914 4121 4731 5001 5116
Size: 914 637 545 325 207 610 270 115
Cuts at: 5116 5251 5402 6436 7514 7942 8536 8762 8914
Size: 135 151 1034 1078 428 594 226 152
Cuts at: 8914 11023 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1078 1034 914 637 610 594
545 428 325 270 226 207 152 151
135 115
AseI AT'TA_AT
Cuts at: 160 1032 2699 8040 9781 160
Size: 872 1667 5341 1741 1606
Fragments arranged by size:
5341 1741 1667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11227
AvaI C'yCGr_G
Cuts at: 1870 1943 8552 1870
Size: 73 6609 4545
Fragments arranged by size:
6609 4545 73
AvrII C'CTAG_G
Cuts at: 6382 6382
Size: 11227
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3798 3831 3798
Size: 33 11194
BamHI G'GATC_C
Cuts at: 2894 6713 8557 8924 2894
Size: 3819 1844 367 5197
Fragments arranged by size:
5197 3819 1844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3331 3575 5315 8548 10003
Size: 325 217 627 1544 244 1740 3233 1455
Cuts at: 10003 618
Size: 1842
Fragments arranged by size:
3233 1842 1740 1544 1455 627 325 244
217
BanII G_rGCy'C
Cuts at: 1391 7489 8546 1391
Size: 6098 1057 4072
Fragments arranged by size:
6098 4072 1057
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 2271 2958 928
Size: 971 261 111 687 9197
Fragments arranged by size:
9197 971 687 261 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9418 9452 1903
Size: 34 7481 34 3678
Fragments arranged by size:
7481 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4515 4579 6918 7376 7858 9119 10329 4515
Size: 64 2339 458 482 1261 1210 5413
Fragments arranged by size:
5413 2339 1261 1210 482 458 64
BclI T'GATC_A
Cuts at: 1548 7399 1548
Size: 5851 5376
BfrBI ATG'CAT
Cuts at: 3571 4100 3571
Size: 529 10698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9838 136
Size: 107 122 71 631 605 8166 1525
Fragments arranged by size:
8166 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4489 6175 7429 11222 4489
Size: 1686 1254 3793 4494
Fragments arranged by size:
4494 3793 1686 1254
BlpI GC'TnA_GC
Cuts at: 8644 8644
Size: 11227
Bme1580I G_kGCm'C
Cuts at: 1790 4978 5594 9288 10444 1790
Size: 3188 616 3694 1156 2573
Fragments arranged by size:
3694 3188 2573 1156 616
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2265 3748 7159
Size: 416 53 83 399 24 848 1483 3411
Cuts at: 7159 8958 9916 442
Size: 1799 958 1753
Fragments arranged by size:
3411 1799 1753 1483 958 848 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3492 4617 6819 7290 8067 9887 1239
Size: 2253 1125 2202 471 777 1820 2579
Fragments arranged by size:
2579 2253 2202 1820 1125 777 471
Bpu10I CC'TnA_GC
Cuts at: 3366 6520 7546 3366
Size: 3154 1026 7047
Fragments arranged by size:
7047 3154 1026
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3284 5079 6335 8247 9352 10217 10515 3284
Size: 1795 1256 1912 1105 865 298 3996
Fragments arranged by size:
3996 1912 1795 1256 1105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9890 882
Size: 617 8391 2219
Fragments arranged by size:
8391 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2417 5669 6906 8314 9018 493
Size: 711 1213 3252 1237 1408 704 2702
Fragments arranged by size:
3252 2702 1408 1237 1213 711 704
BsaBI GATnn'nnATC
Cuts at: 11221 11221
Size: 11227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5722 9034 9416 10051
Size: 53 83 186 936 4189 3312 382 635
Cuts at: 10051 275
Size: 1451
Fragments arranged by size:
4189 3312 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 9660 10058 10332 10479 10890 9660
Size: 398 274 147 411 9997
Fragments arranged by size:
9997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4590 4620 5739 5769 7021
Size: 30 165 30 3187 30 1119 30 1252
Cuts at: 7021 7051 8053 8083 1178
Size: 30 1002 30 4322
Fragments arranged by size:
4322 3187 1252 1119 1002 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3552 3657 5046 5055 5840 7029 1186
Size: 2366 105 1389 9 785 1189 5384
Fragments arranged by size:
5384 2366 1389 1189 785 105 9
BseYI C'CCAG_C
Cuts at: 2839 6429 6788 7128 10430 10926 2839
Size: 3590 359 340 3302 496 3140
Fragments arranged by size:
3590 3302 3140 496 359 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4040 1163
Size: 573 2304 8350
Fragments arranged by size:
8350 2304 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8677 9438 10466 1540
Size: 275 186 6676 761 1028 2301
Fragments arranged by size:
6676 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3169 4978 5095 5594 5828 6971 9288 9373 10444
Size: 1809 117 499 234 1143 2317 85 1071
Cuts at: 10444 3169
Size: 3952
Fragments arranged by size:
3952 2317 1809 1143 1071 499 234 117
85
BsmI GAATG_Cn'
Cuts at: 2351 2666 3155 5850 7728 8738 8831 2351
Size: 315 489 2695 1878 1010 93 4747
Fragments arranged by size:
4747 2695 1878 1010 489 315 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2997 2997
Size: 11227
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3169 4978 5095 5594 5828 6971 7489
Size: 399 1379 1809 117 499 234 1143 518
Cuts at: 7489 8546 9288 9373 10444 1391
Size: 1057 742 85 1071 2174
Fragments arranged by size:
2174 1809 1379 1143 1071 1057 742 518
499 399 234 117 85
BspEI T'CCGG_A
Cuts at: 10890 10890
Size: 11227
BspHI T'CATG_A
Cuts at: 5391 6249 8158 9116 10846 5391
Size: 858 1909 958 1730 5772
Fragments arranged by size:
5772 1909 1730 958 858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5092 5188 6922 8216 8582 844
Size: 4248 96 1734 1294 366 3489
Fragments arranged by size:
4248 3489 1734 1294 366 96
BsrBI CCG'CTC
Cuts at: 1394 1823 9114 1394
Size: 429 7291 3507
Fragments arranged by size:
7291 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2527 3989 5698 7568 7758 9722 9896
Size: 1183 1279 1462 1709 1870 190 1964 174
Cuts at: 9896 65
Size: 1396
Fragments arranged by size:
1964 1870 1709 1462 1396 1279 1183 190
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9871 10058 1700
Size: 110 8061 187 2869
Fragments arranged by size:
8061 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 7063 7555 8074 96
Size: 6967 492 519 3249
Fragments arranged by size:
6967 3249 519 492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11227
BssSI C'ACGA_G
Cuts at: 5067 9287 10299 5067
Size: 4220 1012 5995
Fragments arranged by size:
5995 4220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4067 5697 794
Size: 3273 1630 6324
Fragments arranged by size:
6324 3273 1630
BstBI TT'CG_AA
Cuts at: 5842 10031 5842
Size: 4189 7038
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11227
BstXI CCAn_nnnn'nTGG
Cuts at: 3095 3095
Size: 11227
BstYI r'GATC_y
Cuts at: 1972 2894 2953 3703 4394 4489 5341 5629 6095
Size: 922 59 750 691 95 852 288 466
Cuts at: 6095 6175 6260 6713 7429 8087 8557 8924 9309
Size: 80 85 453 716 658 470 367 385
Cuts at: 9309 9326 10778 10864 10876 11222 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 922 852 750 716 691 658
470 466 453 385 367 346 288 95
86 85 80 59 17 12
BstZ17I GTA'TAC
Cuts at: 2563 2563
Size: 11227
Bsu36I CC'TnA_GG
Cuts at: 2997 8393 2997
Size: 5396 5831
BtgI C'CryG_G
Cuts at: 513 1065 3091 7233 7574 8101 513
Size: 552 2026 4142 341 527 3639
Fragments arranged by size:
4142 3639 2026 552 527 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10488
Fragments arranged by size:
10488 683 56
BtsI GCAGTG_nn'
Cuts at: 4482 4923 4978 5828 5993 8743 9537 9557 4482
Size: 441 55 850 165 2750 794 20 6152
Fragments arranged by size:
6152 2750 850 794 441 165 55 20
ClaI AT'CG_AT
Cuts at: 1554 7470 1554
Size: 5916 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3108 3143 4503 4538 529
Size: 35 2544 35 1360 35 7218
Fragments arranged by size:
7218 2544 1360 35 35 35
DraI TTT'AAA
Cuts at: 3999 5260 7137 8531 3999
Size: 1261 1877 1394 6695
Fragments arranged by size:
6695 1877 1394 1261
DraIII CAC_nnn'GTG
Cuts at: 2935 2935
Size: 11227
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4334 8466 10234 809
Size: 507 3018 4132 1768 1802
Fragments arranged by size:
4132 3018 1802 1768 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5956 8674 9563
Size: 54 1425 50 275 18 4126 2718 889
Cuts at: 9563 11192 8
Size: 1629 43
Fragments arranged by size:
4126 2718 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8674 1537
Size: 275 6862 4090
Fragments arranged by size:
6862 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2230 2571 4367 4803 5670 5862 6063
Size: 713 164 341 1796 436 867 192 201
Cuts at: 6063 6982 7038 9157 1353
Size: 919 56 2119 3423
Fragments arranged by size:
3423 2119 1796 919 867 713 436 341
201 192 164 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9814 10188 10334 387
Size: 1064 138 8225 374 146 1280
Fragments arranged by size:
8225 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2775 3036 3492 4617 4845 5223 5365
Size: 393 1143 261 456 1125 228 378 142
Cuts at: 5365 5843 5855 6540 6561 6819 7290 7905 8067
Size: 478 12 685 21 258 471 615 162
Cuts at: 8067 8165 8304 8367 9290 9887 10674 10942 1239
Size: 98 139 63 923 597 787 268 1524
Fragments arranged by size:
1524 1143 1125 923 787 685 615 597
478 471 456 393 378 268 261 258
228 162 142 139 98 63 21 12
EcoO109I rG'GnC_Cy
Cuts at: 4865 5921 6386 4865
Size: 1056 465 9706
Fragments arranged by size:
9706 1056 465
EcoRI G'AATT_C
Cuts at: 4121 5001 8536 8914 4121
Size: 880 3535 378 6434
Fragments arranged by size:
6434 3535 880 378
EcoRV GAT'ATC
Cuts at: 5446 8035 5446
Size: 2589 8638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6477 6477
Size: 11227
FspI TGC'GCA
Cuts at: 9733 9733
Size: 11227
HaeII r_GCGC'y
Cuts at: 1678 10374 1678
Size: 8696 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3008 3040 3483 3490 3515
Size: 32 135 32 1436 32 443 7 25
Cuts at: 3515 3522 3734 3766 4184 4216 4223 4255 4441
Size: 7 212 32 418 32 7 32 186
Cuts at: 4441 4454 4473 4486 5726 5758 6760 6792 7021
Size: 13 19 13 1240 32 1002 32 229
Cuts at: 7021 7053 7778 7810 8119 8151 8447 8479 9868
Size: 32 725 32 309 32 296 32 1389
Cuts at: 9868 9900 9942 9974 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1436 1389 1240 1002 725 443 418
309 296 229 212 186 135 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 25 19 13
13 7 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3677 6212 6237 7797 8571 8817
Size: 988 281 1739 2535 25 1560 774 246
Cuts at: 8817 669
Size: 3079
Fragments arranged by size:
3079 2535 1739 1560 988 774 281 246
25
HindIII A'AGCT_T
Cuts at: 748 6163 6353 748
Size: 5415 190 5622
Fragments arranged by size:
5622 5415 190
HpaI GTT'AAC
Cuts at: 6237 8817 6237
Size: 2580 8647
KpnI G_GTAC'C
Cuts at: 8552 8552
Size: 11227
MfeI C'AATT_G
Cuts at: 5659 6918 8826 5659
Size: 1259 1908 8060
Fragments arranged by size:
8060 1908 1259
MluI A'CGCG_T
Cuts at: 11103 11103
Size: 11227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3913 4811 4819 5057 5305 5369 6238 6660
Size: 2608 898 8 238 248 64 869 422
Cuts at: 6660 7151 7208 7472 8084 10341 10525 1305
Size: 491 57 264 612 2257 184 2007
Fragments arranged by size:
2608 2257 2007 898 869 612 491 422
264 248 238 184 64 57 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 9405
Fragments arranged by size:
9405 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3093 3438 3581 3624 5152 5526 6834
Size: 648 1927 345 143 43 1528 374 1308
Cuts at: 6834 7573 8106 9185 9544 9703 518
Size: 739 533 1079 359 159 2042
Fragments arranged by size:
2042 1927 1528 1308 1079 739 648 533
374 359 345 159 143 43
MspA1I CmG'CkG
Cuts at: 1606 3224 4434 4992 6320 6788 8643 9320 10468
Size: 1618 1210 558 1328 468 1855 677 1148
Cuts at: 10468 10713 1606
Size: 245 2120
Fragments arranged by size:
2120 1855 1618 1328 1210 1148 677 558
468 245
NcoI C'CATG_G
Cuts at: 513 1065 3091 7233 7574 8101 513
Size: 552 2026 4142 341 527 3639
Fragments arranged by size:
4142 3639 2026 552 527 341
NdeI CA'TA_TG
Cuts at: 387 4096 387
Size: 3709 7518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11227
NotI GC'GGCC_GC
Cuts at: 8674 8674
Size: 11227
NsiI A_TGCA'T
Cuts at: 3573 4102 3573
Size: 529 10698
NspI r_CATG'y
Cuts at: 1132 1229 2847 5157 5525 5743 6899 7690 8259
Size: 97 1618 2310 368 218 1156 791 569
Cuts at: 8259 8585 10130 1132
Size: 326 1545 2229
Fragments arranged by size:
2310 2229 1618 1545 1156 791 569 368
326 218 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11227
PciI A'CATG_T
Cuts at: 5521 5739 7686 10126 5521
Size: 218 1947 2440 6622
Fragments arranged by size:
6622 2440 1947 218
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6686 1108
Size: 5578 5649
PfoI T'CCnGG_A
Cuts at: 8083 8083
Size: 11227
PmeI GTTT'AAAC
Cuts at: 8531 8531
Size: 11227
PmlI CAC'GTG
Cuts at: 8314 8314
Size: 11227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4590 4622 9296 9328 10809 10841 4590
Size: 32 4674 32 1481 32 4976
Fragments arranged by size:
4976 4674 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5921 6386 5921
Size: 465 10762
PshAI GACnn'nnGTC
Cuts at: 1613 6384 1613
Size: 4771 6456
PsiI TTA'TAA
Cuts at: 8797 8797
Size: 11227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 11195
PstI C_TGCA'G
Cuts at: 830 2803 4489 6374 6532 6712 7717 8579 830
Size: 1973 1686 1885 158 180 1005 862 3478
Fragments arranged by size:
3478 1973 1885 1686 1005 862 180 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11227
PvuII CAG'CTG
Cuts at: 3224 4434 6320 6788 3224
Size: 1210 1886 468 7663
Fragments arranged by size:
7663 1886 1210 468
SalI G'TCGA_C
Cuts at: 1936 8569 1936
Size: 6633 4594
SapI GCTCTTCn'nnn_
Cuts at: 2571 5670 6063 2571
Size: 3099 393 7735
Fragments arranged by size:
7735 3099 393
SbfI CC_TGCA'GG
Cuts at: 8579 8579
Size: 11227
ScaI AGT'ACT
Cuts at: 3082 7054 8131 9475 11187 3082
Size: 3972 1077 1344 1712 3122
Fragments arranged by size:
3972 3122 1712 1344 1077
SfcI C'TryA_G
Cuts at: 826 1045 2799 3783 3792 4485 4669 6370 6528
Size: 219 1754 984 9 693 184 1701 158
Cuts at: 6528 6708 6779 7523 7713 8575 9710 10391 10582
Size: 180 71 744 190 862 1135 681 191
Cuts at: 10582 826
Size: 1471
Fragments arranged by size:
1754 1701 1471 1135 984 862 744 693
681 219 191 190 184 180 158 71
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11227
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11227
SmaI CCC'GGG
Cuts at: 1872 8554 1872
Size: 6682 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 3299 5094 6350 8262 8291
Size: 197 926 243 1113 1795 1256 1912 29
Cuts at: 8291 9331 10232 10494 820
Size: 1040 901 262 1553
Fragments arranged by size:
1912 1795 1553 1256 1113 1040 926 901
262 243 197 29
SnaBI TAC'GTA
Cuts at: 493 2417 9018 493
Size: 1924 6601 2702
Fragments arranged by size:
6601 2702 1924
SpeI A'CTAG_T
Cuts at: 152 4283 152
Size: 4131 7096
SphI G_CATG'C
Cuts at: 1132 2847 8585 1132
Size: 1715 5738 3774
Fragments arranged by size:
5738 3774 1715
SspI AAT'ATT
Cuts at: 5 52 8176 9151 5
Size: 47 8124 975 2081
Fragments arranged by size:
8124 2081 975 47
StuI AGG'CCT
Cuts at: 1517 8297 1517
Size: 6780 4447
StyI C'CwwG_G
Cuts at: 513 1065 3091 3708 5039 5048 5906 6382 7233
Size: 552 2026 617 1331 9 858 476 851
Cuts at: 7233 7574 8101 8197 8666 513
Size: 341 527 96 469 3074
Fragments arranged by size:
3074 2026 1331 858 851 617 552 527
476 469 341 96 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 8376 9254 9271 9424 9609 10054
Size: 1255 1332 5464 878 17 153 185 445
Cuts at: 10054 325
Size: 1498
Fragments arranged by size:
5464 1498 1332 1255 878 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2197 3080 6121
Size: 274 80 33 51 1443 220 883 3041
Cuts at: 6121 7052 7063 7555 7874 8074 8129 8412 9473
Size: 931 11 492 319 200 55 283 1061
Cuts at: 9473 11185 96
Size: 1712 138
Fragments arranged by size:
3041 1712 1443 1061 931 883 492 319
283 274 220 200 138 80 55 51
33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2938 2970 4189 4221 5053 5085 5780 5812 5985
Size: 32 1219 32 832 32 695 32 173
Cuts at: 5985 6017 11187 11219 2938
Size: 32 5170 32 2946
Fragments arranged by size:
5170 2946 1219 832 695 173 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11227
XbaI T'CTAG_A
Cuts at: 4609 7015 8563 4609
Size: 2406 1548 7273
Fragments arranged by size:
7273 2406 1548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6013 7942 8238 6013
Size: 1929 296 9002
Fragments arranged by size:
9002 1929 296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11227
XmaI C'CCGG_G
Cuts at: 1870 8552 1870
Size: 6682 4545
XmnI GAAnn'nnTTC
Cuts at: 2717 4621 5413 9356 2717
Size: 1904 792 3943 4588
Fragments arranged by size:
4588 3943 1904 792
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9035 276
Size: 53 83 186 936 7501 2468
Fragments arranged by size:
7501 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BplI BsiWI EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI