Restriction map for
pFN21AE2301
(Circular) (Six-base) MAPSORT of: pFN21AE2301.seq Check: 5714 from: 1 to: 11236
pFN21AE2301 11236 bp
With 182 enzymes: SgfI *
December 2, 2010 09:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5631 7544 7983 5631
Size: 1913 439 8884
Fragments arranged by size:
8884 1913 439
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9046 278
Size: 53 83 186 936 7510 2468
Fragments arranged by size:
7510 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3845 6714 7033 8579 1937
Size: 1908 2869 319 1546 4594
Fragments arranged by size:
4594 2869 1908 1546 319
Acc65I G'GTAC_C
Cuts at: 7886 8557 7886
Size: 671 10565
AclI AA'CG_TT
Cuts at: 1563 5887 9363 9736 1563
Size: 4324 3476 373 3063
Fragments arranged by size:
4324 3476 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2206 3406 3972 4485 4503 4966 8414
Size: 434 140 1200 566 513 18 463 3448
Cuts at: 8414 9299 10683 10951 1632
Size: 885 1384 268 1917
Fragments arranged by size:
3448 1917 1384 1200 885 566 513 463
434 268 140 18
AfeI AGC'GCT
Cuts at: 4910 6269 4910
Size: 1359 9877
AflII C'TTAA_G
Cuts at: 820 1017 5291 5894 820
Size: 197 4274 603 6162
Fragments arranged by size:
6162 4274 603 197
AflIII A'CryG_T
Cuts at: 1203 2539 3840 4195 6328 6464 6795 6953 10135
Size: 1336 1301 355 2133 136 331 158 3182
Cuts at: 10135 11112 1203
Size: 977 1327
Fragments arranged by size:
3182 2133 1336 1327 1301 977 355 331
158 136
AgeI A'CCGG_T
Cuts at: 4884 10067 4884
Size: 5183 6053
AhdI GACnn_n'nnGTC
Cuts at: 5823 6721 9965 5823
Size: 898 3244 7094
Fragments arranged by size:
7094 3244 898
AleI CACnn'nnGTG
Cuts at: 1166 5187 6746 1166
Size: 4021 1559 5656
Fragments arranged by size:
5656 4021 1559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2193 3273 4821 5939 7728 1184
Size: 1009 1080 1548 1118 1789 4692
Fragments arranged by size:
4692 1789 1548 1118 1080 1009
AlwNI CAG_nnn'CTG
Cuts at: 2459 2630 3724 4373 4693 5862 6373 6919 7606
Size: 171 1094 649 320 1169 511 546 687
Cuts at: 7606 7880 8065 8266 8979 10551 2459
Size: 274 185 201 713 1572 3144
Fragments arranged by size:
3144 1572 1169 1094 713 687 649 546
511 320 274 201 185 171
ApaI G_GGCC'C
Cuts at: 2667 5512 7421 2667
Size: 2845 1909 6482
Fragments arranged by size:
6482 2845 1909
ApaLI G'TGCA_C
Cuts at: 6524 8000 9293 10449 6524
Size: 1476 1293 1156 7311
Fragments arranged by size:
7311 1476 1293 1156
ApoI r'AATT_y
Cuts at: 1493 2861 3779 4967 8545 8771 8923 11032 1493
Size: 1368 918 1188 3578 226 152 2109 1697
Fragments arranged by size:
3578 2109 1697 1368 1188 918 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9790 160
Size: 872 8758 1606
Fragments arranged by size:
8758 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11236
AvaI C'yCGr_G
Cuts at: 1870 1943 3864 6939 7149 7420 8561 1870
Size: 73 1921 3075 210 271 1141 4545
Fragments arranged by size:
4545 3075 1921 1141 271 210 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7878 7911 7878
Size: 33 11203
BamHI G'GATC_C
Cuts at: 8566 8933 8566
Size: 367 10869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 5056 5196 7717 7886
Size: 325 217 627 582 2687 140 2521 169
Cuts at: 7886 7894 8557 10012 618
Size: 8 663 1455 1842
Fragments arranged by size:
2687 2521 1842 1455 663 627 582 325
217 169 140 8
BanII G_rGCy'C
Cuts at: 1391 2667 3889 3990 4980 5512 6738 6971 7302
Size: 1276 1222 101 990 532 1226 233 331
Cuts at: 7302 7421 8555 1391
Size: 119 1134 4072
Fragments arranged by size:
4072 1276 1226 1222 1134 990 532 331
233 119 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6072 928
Size: 971 4173 6092
Fragments arranged by size:
6092 4173 971
BbvCI CC'TCA_GC
Cuts at: 3006 3063 3089 3006
Size: 57 26 11153
Fragments arranged by size:
11153 57 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6844 6878 9427 9461 1903
Size: 34 4907 34 2549 34 3678
Fragments arranged by size:
4907 3678 2549 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 3721 4925 9128 10338 3107
Size: 614 1204 4203 1210 4005
Fragments arranged by size:
4203 4005 1210 1204 614
BclI T'GATC_A
Cuts at: 1548 6102 1548
Size: 4554 6682
BfrBI ATG'CAT
Cuts at: 6592 6592
Size: 11236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 9847 136
Size: 107 122 71 631 605 337 7838 1525
Fragments arranged by size:
7838 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3489 5437 7056 11231 3489
Size: 1948 1619 4175 3494
Fragments arranged by size:
4175 3494 1948 1619
BlpI GC'TnA_GC
Cuts at: 3618 4719 6254 6761 8653 3618
Size: 1101 1535 507 1892 6201
Fragments arranged by size:
6201 1892 1535 1101 507
Bme1580I G_kGCm'C
Cuts at: 1790 2667 3062 5059 5199 5512 5950 6528 7421
Size: 877 395 1997 140 313 438 578 893
Cuts at: 7421 7722 7899 8004 8110 9297 10453 1790
Size: 301 177 105 106 1187 1156 2573
Fragments arranged by size:
2573 1997 1187 1156 893 877 578 438
395 313 301 177 140 106 105
BmgBI CAC'GTC
Cuts at: 1318 5185 1318
Size: 3867 7369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 5927 7329
Size: 416 53 83 399 24 2264 2246 1402
Cuts at: 7329 7983 8392 8404 8505 8967 9925 442
Size: 654 409 12 101 462 958 1753
Fragments arranged by size:
2264 2246 1753 1402 958 654 462 416
409 399 101 83 53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 6148 3511
Size: 2637 8599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2826 3611 4428 4583 6032 6710 7241 7278
Size: 1587 785 817 155 1449 678 531 37
Cuts at: 7278 7650 7733 7894 9896 1239
Size: 372 83 161 2002 2579
Fragments arranged by size:
2579 2002 1587 1449 817 785 678 531
372 161 155 83 37
Bpu10I CC'TnA_GC
Cuts at: 3006 3063 3089 4542 8151 3006
Size: 57 26 1453 3609 6091
Fragments arranged by size:
6091 3609 1453 57 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 2801 5660 9361 10226 10524 2044
Size: 757 2859 3701 865 298 2756
Fragments arranged by size:
3701 2859 2756 865 757 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 4404 4552 9899 882
Size: 617 1014 1891 148 5347 2219
Fragments arranged by size:
5347 2219 1891 1014 617 148
BsaAI yAC'GTr
Cuts at: 493 1204 4198 5848 6954 9027 493
Size: 711 2994 1650 1106 2073 2702
Fragments arranged by size:
2994 2702 2073 1650 1106 711
BsaBI GATnn'nnATC
Cuts at: 6698 11230 6698
Size: 4532 6704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4863 7656 8115 8127
Size: 53 83 186 936 3330 2793 459 12
Cuts at: 8127 9043 9425 10060 275
Size: 916 382 635 1451
Fragments arranged by size:
3330 2793 1451 936 916 635 459 382
186 83 53 12
BsaWI w'CCGG_w
Cuts at: 4884 7456 9669 10067 10341 10488 10899 4884
Size: 2572 2213 398 274 147 411 5221
Fragments arranged by size:
5221 2572 2213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2986 3016 3721 3751 5779
Size: 30 165 30 1583 30 705 30 2028
Cuts at: 5779 5809 6872 6902 7438 7468 8080 8110 1178
Size: 30 1063 30 536 30 612 30 4304
Fragments arranged by size:
4304 2028 1583 1063 705 612 536 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2994 3484 4247 4888 5299 5333 5596 5771
Size: 1808 490 763 641 411 34 263 175
Cuts at: 5771 5774 5781 6674 7167 7172 7265 7666 8370
Size: 3 7 893 493 5 93 401 704
Cuts at: 8370 1186
Size: 4052
Fragments arranged by size:
4052 1808 893 763 704 641 493 490
411 401 263 175 93 34 7 5
3
BseYI C'CCAG_C
Cuts at: 2059 5052 6021 6227 6344 6657 7799 7901 8391
Size: 2993 969 206 117 313 1142 102 490
Cuts at: 8391 8403 8439 8499 10439 10935 2059
Size: 12 36 60 1940 496 2360
Fragments arranged by size:
2993 2360 1940 1142 969 496 490 313
206 117 102 60 36 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2307 4220 4634 4643 5259 5292 6665
Size: 573 571 1913 414 9 616 33 1373
Cuts at: 6665 7451 1163
Size: 786 4948
Fragments arranged by size:
4948 1913 1373 786 616 573 571 414
33 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2054 5617 8686 9447 10475 1540
Size: 275 186 53 3563 3069 761 1028 2301
Fragments arranged by size:
3563 3069 2301 1028 761 275 186 53
BsiHKAI G_wGCw'C
Cuts at: 3694 3942 4980 6255 6528 6738 6771 7931 8004
Size: 248 1038 1275 273 210 33 1160 73
Cuts at: 8004 9297 9382 10453 3694
Size: 1293 85 1071 4477
Fragments arranged by size:
4477 1293 1275 1160 1071 1038 273 248
210 85 73 33
BsmI GAATG_Cn'
Cuts at: 2751 6042 8747 8840 2751
Size: 3291 2705 93 5147
Fragments arranged by size:
5147 3291 2705 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2667 3062 3694 3889 3942 3990 4980
Size: 399 877 395 632 195 53 48 990
Cuts at: 4980 5059 5199 5512 5950 6255 6528 6738 6771
Size: 79 140 313 438 305 273 210 33
Cuts at: 6771 6971 7302 7421 7722 7899 7931 8004 8110
Size: 200 331 119 301 177 32 73 106
Cuts at: 8110 8555 9297 9382 10453 1391
Size: 445 742 85 1071 2174
Fragments arranged by size:
2174 1071 990 877 742 632 445 438
399 395 331 313 305 301 273 210
200 195 177 140 119 106 85 79
73 53 48 33 32
BspEI T'CCGG_A
Cuts at: 7456 10899 7456
Size: 3443 7793
BspHI T'CATG_A
Cuts at: 5067 5377 6099 9125 10855 5067
Size: 310 722 3026 1730 5448
Fragments arranged by size:
5448 3026 1730 722 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 3222 3241 5528 5631 5931 7544 7558
Size: 2213 165 19 2287 103 300 1613 14
Cuts at: 7558 7720 7874 7983 8591 844
Size: 162 154 109 608 3489
Fragments arranged by size:
3489 2287 2213 1613 608 300 165 162
154 109 103 19 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3295 3772 5604 7431 9123 1394
Size: 429 1472 477 1832 1827 1692 3507
Fragments arranged by size:
3507 1832 1827 1692 1472 477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3518 5176 5356 5923 6297 9731 9905
Size: 1183 2270 1658 180 567 374 3434 174
Cuts at: 9905 65
Size: 1396
Fragments arranged by size:
3434 2270 1658 1396 1183 567 374 180
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2619 2889 3069 3173 4458 4884
Size: 110 227 582 270 180 104 1285 426
Cuts at: 4884 5249 7137 7698 9880 10067 1700
Size: 365 1888 561 2182 187 2869
Fragments arranged by size:
2869 2182 1888 1285 582 561 426 365
270 227 187 180 110 104
BsrGI T'GTAC_A
Cuts at: 96 2906 4938 7527 96
Size: 2810 2032 2589 3805
Fragments arranged by size:
3805 2810 2589 2032
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11236
BssSI C'ACGA_G
Cuts at: 4573 7563 9296 10308 4573
Size: 2990 1733 1012 5501
Fragments arranged by size:
5501 2990 1733 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4621 5425 5862 6589 7171 7606 8266 794
Size: 3827 804 437 727 582 435 660 3764
Fragments arranged by size:
3827 3764 804 727 660 582 437 435
BstBI TT'CG_AA
Cuts at: 10040 10040
Size: 11236
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11236
BstXI CCAn_nnnn'nTGG
Cuts at: 3589 5576 7107 3589
Size: 1987 1531 7718
Fragments arranged by size:
7718 1987 1531
BstYI r'GATC_y
Cuts at: 1972 2019 2796 3489 3979 4741 5437 7056 7069
Size: 47 777 693 490 762 696 1619 13
Cuts at: 7069 7960 8262 8566 8933 9318 9335 10787 10873
Size: 891 302 304 367 385 17 1452 86
Cuts at: 10873 10885 11231 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1619 1452 891 777 762 696 693
490 385 367 346 304 302 86 47
17 13 12
BstZ17I GTA'TAC
Cuts at: 3846 3846
Size: 11236
Bsu36I CC'TnA_GG
Cuts at: 2082 7193 2082
Size: 5111 6125
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 6666 7890 513
Size: 552 939 50 4612 1224 3859
Fragments arranged by size:
4612 3859 1224 939 552 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3646 5209 5395 6133 487
Size: 683 56 2420 1563 186 738 5590
Fragments arranged by size:
5590 2420 1563 738 683 186 56
BtsI GCAGTG_nn'
Cuts at: 2182 2705 4476 5426 5782 6923 7226 7232 8063
Size: 523 1771 950 356 1141 303 6 831
Cuts at: 8063 8752 9546 9566 2182
Size: 689 794 20 3852
Fragments arranged by size:
3852 1771 1141 950 831 794 689 523
356 303 20 6
ClaI AT'CG_AT
Cuts at: 1554 6978 1554
Size: 5424 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11201
DraI TTT'AAA
Cuts at: 4959 8540 4959
Size: 3581 7655
DraIII CAC_nnn'GTG
Cuts at: 5270 6672 5270
Size: 1402 9834
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10243 809
Size: 507 8927 1802
Fragments arranged by size:
8927 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2051 2621 2721
Size: 54 1425 50 275 18 221 570 100
Cuts at: 2721 5797 8683 9572 11201 8
Size: 3076 2886 889 1629 43
Fragments arranged by size:
3076 2886 1629 1425 889 570 275 221
100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2051 8683 1537
Size: 275 239 6632 4090
Fragments arranged by size:
6632 4090 275 239
EarI CTCTTCn'nnn_
Cuts at: 1353 2418 2528 3590 4985 6388 6410 6743 7810
Size: 1065 110 1062 1395 1403 22 333 1067
Cuts at: 7810 9166 1353
Size: 1356 3423
Fragments arranged by size:
3423 1403 1395 1356 1067 1065 1062 333
110 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2789 3620 9823 10197 10343 387
Size: 1064 138 1200 831 6203 374 146 1280
Fragments arranged by size:
6203 1280 1200 1064 831 374 146 138
EcoICRI GAG'CTC
Cuts at: 4978 6736 4978
Size: 1758 9478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2206 2826 3406 3611 3972 4428
Size: 393 434 140 620 580 205 361 456
Cuts at: 4428 4485 4503 4583 4966 6032 6710 7241 7278
Size: 57 18 80 383 1066 678 531 37
Cuts at: 7278 7650 7733 7894 8414 9299 9896 10683 10951
Size: 372 83 161 520 885 597 787 268
Cuts at: 10951 1239
Size: 1524
Fragments arranged by size:
1524 1066 885 787 678 620 597 580
531 520 456 434 393 383 372 361
268 205 161 140 83 80 57 37
18
EcoNI CCTnn'n_nnAGG
Cuts at: 4823 5592 4823
Size: 769 10467
EcoO109I rG'GnC_Cy
Cuts at: 2063 2086 2401 2627 2663 2664 3467 3871 4164
Size: 23 315 226 36 1 803 404 293
Cuts at: 4164 5508 7197 7877 8011 2063
Size: 1344 1689 680 134 5288
Fragments arranged by size:
5288 1689 1344 803 680 404 315 293
226 134 36 23 1
EcoRI G'AATT_C
Cuts at: 3779 8545 8923 3779
Size: 4766 378 6092
Fragments arranged by size:
6092 4766 378
EcoRV GAT'ATC
Cuts at: 4336 4489 4336
Size: 153 11083
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2323 4184 4354 2323
Size: 1861 170 9205
Fragments arranged by size:
9205 1861 170
FspI TGC'GCA
Cuts at: 5855 9742 5855
Size: 3887 7349
HaeII r_GCGC'y
Cuts at: 1678 4912 5962 6271 6886 7735 8422 10383 1678
Size: 3234 1050 309 615 849 687 1961 2531
Fragments arranged by size:
3234 2531 1961 1050 849 687 615 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3739 3771 3815 3847 4883
Size: 32 135 32 2167 32 44 32 1036
Cuts at: 4883 4915 4979 5011 5062 5094 5809 5841 6179
Size: 32 64 32 51 32 715 32 338
Cuts at: 6179 6211 6707 6739 6872 6904 6933 6965 7438
Size: 32 496 32 133 32 29 32 473
Cuts at: 7438 7470 8026 8058 9877 9909 9951 9983 1373
Size: 32 556 32 1819 32 42 32 2626
Fragments arranged by size:
2626 2167 1819 1036 715 556 496 473
338 135 133 64 51 44 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2545 2976 6715 8580 8826 669
Size: 988 281 607 431 3739 1865 246 3079
Fragments arranged by size:
3739 3079 1865 988 607 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11236
HpaI GTT'AAC
Cuts at: 8826 8826
Size: 11236
KpnI G_GTAC'C
Cuts at: 7890 8561 7890
Size: 671 10565
MfeI C'AATT_G
Cuts at: 8835 8835
Size: 11236
MluI A'CGCG_T
Cuts at: 11112 11112
Size: 11236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 3234 5765 6199 6732 6750 7406 10350
Size: 936 993 2531 434 533 18 656 2944
Cuts at: 10350 10534 1305
Size: 184 2007
Fragments arranged by size:
2944 2531 2007 993 936 656 533 434
184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 8523
Fragments arranged by size:
8523 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2486 2729 3523 4803 5187 5810
Size: 648 1009 311 243 794 1280 384 623
Cuts at: 5810 6746 7646 9194 9553 9712 518
Size: 936 900 1548 359 159 2042
Fragments arranged by size:
2042 1548 1280 1009 936 900 794 648
623 384 359 311 243 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2929 3093 3543 4261 4690 6025 6705
Size: 450 873 164 450 718 429 1335 680
Cuts at: 6705 6916 7168 7984 8652 9329 10477 10722 1606
Size: 211 252 816 668 677 1148 245 2120
Fragments arranged by size:
2120 1335 1148 873 816 718 680 677
668 450 450 429 252 245 211 164
NaeI GCC'GGC
Cuts at: 2891 2891
Size: 11236
NcoI C'CATG_G
Cuts at: 513 1065 2004 7890 513
Size: 552 939 5886 3859
Fragments arranged by size:
5886 3859 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11236
NgoMIV G'CCGG_C
Cuts at: 2889 2889
Size: 11236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11236
NotI GC'GGCC_GC
Cuts at: 2051 8683 2051
Size: 6632 4604
NsiI A_TGCA'T
Cuts at: 6594 6594
Size: 11236
NspI r_CATG'y
Cuts at: 1132 1229 2543 3844 4594 4622 6226 6332 6424
Size: 97 1314 1301 750 28 1604 106 92
Cuts at: 6424 6468 6634 6721 6760 6799 7768 8594 10139
Size: 44 166 87 39 39 969 826 1545
Cuts at: 10139 1132
Size: 2229
Fragments arranged by size:
2229 1604 1545 1314 1301 969 826 750
166 106 97 92 87 44 39 39
28
PasI CC'CwG_GG
Cuts at: 1348 7065 7828 8110 1348
Size: 5717 763 282 4474
Fragments arranged by size:
5717 4474 763 282
PciI A'CATG_T
Cuts at: 2539 3840 6328 6464 6795 10135 2539
Size: 1301 2488 136 331 3340 3640
Fragments arranged by size:
3640 3340 2488 1301 331 136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4264 5626 6055 6672 7481 1108
Size: 3156 1362 429 617 809 4863
Fragments arranged by size:
4863 3156 1362 809 617 429
PfoI T'CCnGG_A
Cuts at: 3588 3702 4405 4550 5430 6687 3588
Size: 114 703 145 880 1257 8137
Fragments arranged by size:
8137 1257 880 703 145 114
PmeI GTTT'AAAC
Cuts at: 8540 8540
Size: 11236
PmlI CAC'GTG
Cuts at: 4198 6954 4198
Size: 2756 8480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9305 9337 10818 10850 9305
Size: 32 1481 32 9691
Fragments arranged by size:
9691 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 2401 3467 3871 4164 2086
Size: 315 1066 404 293 9158
Fragments arranged by size:
9158 1066 404 315 293
PshAI GACnn'nnGTC
Cuts at: 1613 2271 7655 1613
Size: 658 5384 5194
Fragments arranged by size:
5384 5194 658
PsiI TTA'TAA
Cuts at: 7683 8806 7683
Size: 1123 10113
PspOMI G'GGCC_C
Cuts at: 2663 5508 7417 2663
Size: 2845 1909 6482
Fragments arranged by size:
6482 2845 1909
PspXI vC'TCGA_Gb
Cuts at: 7149 7149
Size: 11236
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5000 5032 5000
Size: 32 11204
PstI C_TGCA'G
Cuts at: 830 2635 2928 3352 3720 4906 5095 6096 6793
Size: 1805 293 424 368 1186 189 1001 697
Cuts at: 6793 7555 7885 8070 8588 830
Size: 762 330 185 518 3478
Fragments arranged by size:
3478 1805 1186 1001 762 697 518 424
368 330 293 189 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11236
PvuII CAG'CTG
Cuts at: 2929 3093 3543 4690 6916 2929
Size: 164 450 1147 2226 7249
Fragments arranged by size:
7249 2226 1147 450 164
RsrII CG'GwC_CG
Cuts at: 4887 4887
Size: 11236
SacI G_AGCT'C
Cuts at: 4980 6738 4980
Size: 1758 9478
SacII CC_GC'GG
Cuts at: 2057 2057
Size: 11236
SalI G'TCGA_C
Cuts at: 1936 6713 8578 1936
Size: 4777 1865 4594
Fragments arranged by size:
4777 4594 1865
SanDI GG'GwC_CC
Cuts at: 4164 4164
Size: 11236
SapI GCTCTTCn'nnn_
Cuts at: 4985 6410 6743 7810 4985
Size: 1425 333 1067 8411
Fragments arranged by size:
8411 1425 1067 333
SbfI CC_TGCA'GG
Cuts at: 2635 7555 7885 8588 2635
Size: 4920 330 703 5283
Fragments arranged by size:
5283 4920 703 330
ScaI AGT'ACT
Cuts at: 2582 3795 6211 6560 7339 9484 11196 2582
Size: 1213 2416 349 779 2145 1712 2622
Fragments arranged by size:
2622 2416 2145 1712 1213 779 349
SexAI A'CCwGG_T
Cuts at: 4530 6618 4530
Size: 2088 9148
SfcI C'TryA_G
Cuts at: 826 1045 2631 2924 3101 3348 3716 4902 4948
Size: 219 1586 293 177 247 368 1186 46
Cuts at: 4948 5091 5155 5589 6092 6514 6789 7034 7504
Size: 143 64 434 503 422 275 245 470
Cuts at: 7504 7551 7881 8066 8584 9719 10400 10591 826
Size: 47 330 185 518 1135 681 191 1471
Fragments arranged by size:
1586 1471 1186 1135 681 518 503 470
434 422 368 330 293 275 247 245
219 191 185 177 143 64 47 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11236
SmaI CCC'GGG
Cuts at: 1872 7422 8563 1872
Size: 5550 1141 4545
Fragments arranged by size:
5550 4545 1141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2023 2780 5291 5675 5894 7149
Size: 197 926 80 757 2511 384 219 1255
Cuts at: 7149 9340 10241 10503 820
Size: 2191 901 262 1553
Fragments arranged by size:
2511 2191 1553 1255 926 901 757 384
262 219 197 80
SnaBI TAC'GTA
Cuts at: 493 9027 493
Size: 8534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11236
SphI G_CATG'C
Cuts at: 1132 8594 1132
Size: 7462 3774
SspI AAT'ATT
Cuts at: 5 52 4649 9160 5
Size: 47 4597 4511 2081
Fragments arranged by size:
4597 4511 2081 47
StuI AGG'CCT
Cuts at: 1517 5297 7524 1517
Size: 3780 2227 5229
Fragments arranged by size:
5229 3780 2227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2523 3132 4146 4168 4329 4585
Size: 552 939 519 609 1014 22 161 256
Cuts at: 4585 6084 6153 6984 7365 7723 7890 8675 513
Size: 1499 69 831 381 358 167 785 3074
Fragments arranged by size:
3074 1499 1014 939 831 785 609 552
519 381 358 256 167 161 69 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2764 4159 4226 6671 7348 7488 7547
Size: 1255 1184 1395 67 2445 677 140 59
Cuts at: 7547 7946 8092 9263 9280 9433 9618 10063 325
Size: 399 146 1171 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1395 1255 1184 1171 677 445
399 185 153 146 140 67 59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 2906 3793
Size: 274 80 33 51 1443 603 326 887
Cuts at: 3793 4938 5689 6209 6558 7337 7527 9482 11194
Size: 1145 751 520 349 779 190 1955 1712
Cuts at: 11194 96
Size: 138
Fragments arranged by size:
1955 1712 1443 1145 887 779 751 603
520 349 326 274 190 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2986 3018 4201 4233 5779 5811 6362 6394 11196
Size: 32 1183 32 1546 32 551 32 4802
Cuts at: 11196 11228 2986
Size: 32 2994
Fragments arranged by size:
4802 2994 1546 1183 551 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6193 1316
Size: 4877 6359
XbaI T'CTAG_A
Cuts at: 5080 6262 8572 5080
Size: 1182 2310 7744
Fragments arranged by size:
7744 2310 1182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6685 7478 6685
Size: 793 10443
XhoI C'TCGA_G
Cuts at: 1943 7149 1943
Size: 5206 6030
XmaI C'CCGG_G
Cuts at: 1870 7420 8561 1870
Size: 5550 1141 4545
Fragments arranged by size:
5550 4545 1141
XmnI GAAnn'nnTTC
Cuts at: 2865 3813 5748 6906 9365 2865
Size: 948 1935 1158 2459 4736
Fragments arranged by size:
4736 2459 1935 1158 948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9044 276
Size: 53 83 186 936 7510 2468
Fragments arranged by size:
7510 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI BsmBI FseI FspAI
KasI NarI NruI PacI SfiI SfoI SrfI SwaI