Restriction map for
pFN21AE2299
(Circular) (Six-base) MAPSORT of: pFN21AE2299.seq Check: 5355 from: 1 to: 5992
pFN21AE2299 5992 bp
With 182 enzymes: SgfI *
November 10, 2010 12:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2879 2879
Size: 5992
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2306 2939 3335 1937
Size: 369 633 396 4594
Fragments arranged by size:
4594 633 396 369
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2323 4055 5439 5707 1632
Size: 557 134 1732 1384 268 1917
Fragments arranged by size:
1917 1732 1384 557 268 134
AfeI AGC'GCT
Cuts at: 2642 2642
Size: 5992
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2292 4891 5868 1203
Size: 1089 2599 977 1327
Fragments arranged by size:
2599 1327 1089 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2462 4721 2462
Size: 2259 3733
AleI CACnn'nnGTG
Cuts at: 1166 2790 3164 1166
Size: 1624 374 3994
Fragments arranged by size:
3994 1624 374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2982 3139 3735 5307 2982
Size: 157 596 1572 3667
Fragments arranged by size:
3667 1572 596 157
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
AvrII C'CTAG_G
Cuts at: 2423 2423
Size: 5992
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 3235 3313 4768 618
Size: 325 217 627 344 1104 78 1455 1842
Fragments arranged by size:
1842 1455 1104 627 344 325 217 78
BanII G_rGCy'C
Cuts at: 1391 2454 3203 3311 1391
Size: 1063 749 108 4072
Fragments arranged by size:
4072 1063 749 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2764 928
Size: 971 865 4156
Fragments arranged by size:
4156 971 865
BbvCI CC'TCA_GC
Cuts at: 2819 2819
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 4711
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 2448 3409 2448
Size: 961 5031
Bme1580I G_kGCm'C
Cuts at: 1790 2020 3189 3240 4053 5209 1790
Size: 230 1169 51 813 1156 2573
Fragments arranged by size:
2573 1169 1156 813 230 51
BmgBI CAC'GTC
Cuts at: 1318 2295 1318
Size: 977 5015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 3723 4681
Size: 416 53 83 399 24 718 1588 958
Cuts at: 4681 442
Size: 1753
Fragments arranged by size:
1753 1588 958 718 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2468
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2201 2834 4652 1239
Size: 918 44 633 1818 2579
Fragments arranged by size:
2579 1818 918 633 44
Bpu10I CC'TnA_GC
Cuts at: 2151 2819 2151
Size: 668 5324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3134 3230 4117 4982 5280 2840
Size: 294 96 887 865 298 3552
Fragments arranged by size:
3552 887 865 298 294 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 2606 2798 4655 882
Size: 617 1059 48 192 1857 2219
Fragments arranged by size:
2219 1857 1059 617 192 48
BsaAI yAC'GTr
Cuts at: 493 1204 2482 2926 3783 493
Size: 711 1278 444 857 2702
Fragments arranged by size:
2702 1278 857 711 444
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3110 3799 4181 4816
Size: 53 83 186 936 1577 689 382 635
Cuts at: 4816 275
Size: 1451
Fragments arranged by size:
1577 1451 936 689 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2649 4425 4823 5097 5244 5655 2649
Size: 1776 398 274 147 411 2986
Fragments arranged by size:
2986 1776 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5767
Fragments arranged by size:
5767 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2921 1186
Size: 1735 4257
BseYI C'CCAG_C
Cuts at: 2085 2102 2607 2643 3024 5195 5691 2085
Size: 17 505 36 381 2171 496 2386
Fragments arranged by size:
2386 2171 505 496 381 36 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3152 3307 1163
Size: 573 1416 155 3848
Fragments arranged by size:
3848 1416 573 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2454 2856 4053 4138 5209 2157
Size: 297 402 1197 85 1071 2940
Fragments arranged by size:
2940 1197 1071 402 297 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2157 2454 2856 3189 3203 3240
Size: 399 230 137 297 402 333 14 37
Cuts at: 3240 3311 4053 4138 5209 1391
Size: 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 333 297 230
137 85 71 37 14
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2494 3881 5611 2494
Size: 1387 1730 2875
Fragments arranged by size:
2875 1730 1387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 2879 3347 844
Size: 1919 116 468 3489
Fragments arranged by size:
3489 1919 468 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3039 4487 4661 65
Size: 1183 1791 1448 174 1396
Fragments arranged by size:
1791 1448 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 4823 1700
Size: 110 2826 187 2869
Fragments arranged by size:
2869 2826 187 110
BsrGI T'GTAC_A
Cuts at: 96 2280 96
Size: 2184 3808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 2903 4052 5064 2903
Size: 1149 1012 3831
Fragments arranged by size:
3831 1149 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 3177 794
Size: 1607 776 3609
Fragments arranged by size:
3609 1607 776
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2792 3166 2792
Size: 374 5618
BstYI r'GATC_y
Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987
Size: 1350 367 385 17 1452 86 12 346
Cuts at: 5987 1972
Size: 1977
Fragments arranged by size:
1977 1452 1350 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2075 2797 3508 4302 4322 2075
Size: 722 711 794 20 3745
Fragments arranged by size:
3745 794 722 711 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2732 4999 809
Size: 507 1416 2267 1802
Fragments arranged by size:
2267 1802 1416 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3439 4328
Size: 54 1425 50 275 18 1090 519 889
Cuts at: 4328 5957 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1090 889 519 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 3922 1353
Size: 1058 1511 3423
Fragments arranged by size:
3423 1511 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2968 4579 4953 5099 387
Size: 1064 138 1379 1611 374 146 1280
Fragments arranged by size:
1611 1379 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2452 2452
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2189 2201 2323 2834 4055 4652
Size: 393 525 32 12 122 511 1221 597
Cuts at: 4652 5439 5707 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1221 787 597 525 511 393 268
122 32 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3218
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 2227 2227
Size: 5992
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 5992
FspI TGC'GCA
Cuts at: 2207 4498 2207
Size: 2291 3701
HaeII r_GCGC'y
Cuts at: 1678 2634 2644 2967 5139 1678
Size: 956 10 323 2172 2531
Fragments arranged by size:
2531 2172 956 323 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2448 2480 4633
Size: 32 135 32 770 32 74 32 2153
Cuts at: 4633 4665 4707 4739 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2153 770 135 74 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2307 2940 3336 3582 669
Size: 988 281 369 633 396 246 3079
Fragments arranged by size:
3079 988 633 396 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2479 2679 5106 5290 1305
Size: 1041 133 200 2427 184 2007
Fragments arranged by size:
2427 2007 1041 200 184 133
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2922 10
Size: 54 1425 343 1090 3080
Fragments arranged by size:
3080 1425 1090 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2790 3164 3233 3269 3950 4309 4468
Size: 648 1624 374 69 36 681 359 159
Cuts at: 4468 518
Size: 2042
Fragments arranged by size:
2042 1624 681 648 374 359 159 69
36
MspA1I CmG'CkG
Cuts at: 1606 3033 3408 4085 5233 5478 1606
Size: 1427 375 677 1148 245 2120
Fragments arranged by size:
2120 1427 1148 677 375 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5440
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 3350 4895 1132
Size: 97 2121 1545 2229
Fragments arranged by size:
2229 2121 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PmlI CAC'GTG
Cuts at: 2482 2926 2482
Size: 444 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2227
Size: 5992
PshAI GACnn'nnGTC
Cuts at: 1613 2095 1613
Size: 482 5510
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2082 3344 830
Size: 1252 1262 3478
Fragments arranged by size:
3478 1262 1252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 3033 3033
Size: 5992
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 5992
SacI G_AGCT'C
Cuts at: 2454 2454
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 2305 2938 3334 1936
Size: 369 633 396 4594
Fragments arranged by size:
4594 633 396 369
SanDI GG'GwC_CC
Cuts at: 2227 2227
Size: 5992
SapI GCTCTTCn'nnn_
Cuts at: 2411 2411
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 2063 2078 2559 3340 4475 5156 5347
Size: 219 1018 15 481 781 1135 681 191
Cuts at: 5347 826
Size: 1471
Fragments arranged by size:
1471 1135 1018 781 681 481 219 191
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2855 3149 3245 4096 4997 5259
Size: 197 926 912 294 96 851 901 262
Cuts at: 5259 820
Size: 1553
Fragments arranged by size:
1553 926 912 901 851 294 262 197
96
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 3176 1517
Size: 1659 4333
StyI C'CwwG_G
Cuts at: 513 1065 2423 2616 2799 2868 3048 3093 3431
Size: 552 1358 193 183 69 180 45 338
Cuts at: 3431 513
Size: 3074
Fragments arranged by size:
3074 1358 552 338 193 183 180 69
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2014 3061 3273 4019 4036 4189 4374
Size: 1255 434 1047 212 746 17 153 185
Cuts at: 4374 4819 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1047 746 445 434 212 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 2280 4238
Size: 274 80 33 51 1443 288 15 1958
Cuts at: 4238 5950 96
Size: 1712 138
Fragments arranged by size:
1958 1712 1443 288 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2082 2575 2082
Size: 493 5499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI SacII SexAI SfiI SfoI SrfI SwaI