Restriction map for
pFN21AE2298
(Circular) (Six-base) MAPSORT of: pFN21AE2298.seq Check: 6779 from: 1 to: 5977
pFN21AE2298 5977 bp
With 182 enzymes: SgfI *
November 10, 2010 12:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 2487 3320 1937
Size: 214 336 833 4594
Fragments arranged by size:
4594 833 336 214
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4040 5424 5692 1632
Size: 2408 1384 268 1917
Fragments arranged by size:
2408 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2192 820
Size: 197 1175 4605
Fragments arranged by size:
4605 1175 197
AflIII A'CryG_T
Cuts at: 1203 2256 4876 5853 1203
Size: 1053 2620 977 1327
Fragments arranged by size:
2620 1327 1053 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2453 1166
Size: 1287 4690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 3720 5292 3720
Size: 1572 4405
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2276 3142 3286 3512 3664 5773 1493
Size: 783 866 144 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 783 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2373 3192 3296 1391
Size: 621 361 819 104 4072
Fragments arranged by size:
4072 819 621 361 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BbvCI CC'TCA_GC
Cuts at: 2697 2697
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2447 2481 4168 4202 1903
Size: 34 510 34 1687 34 3678
Fragments arranged by size:
3678 1687 510 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3035 3869 5079 3035
Size: 834 1210 3933
Fragments arranged by size:
3933 1210 834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2338 5972 2338
Size: 3634 2343
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2740 3177 4038 5194 1790
Size: 950 437 861 1156 2573
Fragments arranged by size:
2573 1156 950 861 437
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2537 2684 4637 1239
Size: 914 384 147 1953 2579
Fragments arranged by size:
2579 1953 914 384 147
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3122 4102 4967 5265 3122
Size: 980 865 298 3834
Fragments arranged by size:
3834 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3219 3784 4166 4801
Size: 53 83 186 936 1686 565 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1686 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2490 4410 4808 5082 5229 5640 2490
Size: 1920 398 274 147 411 2827
Fragments arranged by size:
2827 1920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 1178
Size: 30 165 30 1593 30 4129
Fragments arranged by size:
4129 1593 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2378 1186
Size: 899 293 4785
Fragments arranged by size:
4785 899 293
BseYI C'CCAG_C
Cuts at: 2349 5180 5676 2349
Size: 2831 496 2650
Fragments arranged by size:
2831 2650 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3062 3488 3581 3062
Size: 426 93 5458
Fragments arranged by size:
5458 426 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3262 3262
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2373 2740 3177 3192 3296 4038
Size: 399 222 361 367 437 15 104 742
Cuts at: 4038 4123 5194 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 367 361 222
104 85 15
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2076 3866 5596 2076
Size: 1790 1730 2457
Fragments arranged by size:
2457 1790 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2986 3864 1394
Size: 429 1163 878 3507
Fragments arranged by size:
3507 1163 878 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2353 4621 4808 1700
Size: 110 543 2268 187 2869
Fragments arranged by size:
2869 2268 543 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2338 3307 3674 4059 4076 5528 5614 5626
Size: 366 969 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 969 385 367 366 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3061 513
Size: 552 939 1057 3429
Fragments arranged by size:
3429 1057 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3013 487
Size: 683 56 1787 3451
Fragments arranged by size:
3451 1787 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2716 2794 2934 3493 4287 4307 2162
Size: 554 78 140 559 794 20 3832
Fragments arranged by size:
3832 794 559 554 140 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3238 3281 3238
Size: 43 5934
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3218 4984 809
Size: 507 1902 1766 1802
Fragments arranged by size:
1902 1802 1766 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3252 3424 4313
Size: 54 1425 50 275 18 1422 172 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1422 889 275 172 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 2412 2886 3907 1353
Size: 1043 16 474 1021 3423
Fragments arranged by size:
3423 1043 1021 474 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3024 4564 4938 5084 387
Size: 1064 138 1435 1540 374 146 1280
Fragments arranged by size:
1540 1435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2537 2684 4040 4637 5424 5692
Size: 393 521 384 147 1356 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 1356 787 597 521 393 384 268
147
EcoO109I rG'GnC_Cy
Cuts at: 2979 2979
Size: 5977
EcoRI G'AATT_C
Cuts at: 3142 3286 3664 3142
Size: 144 378 5455
Fragments arranged by size:
5455 378 144
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2279 2311 2904
Size: 32 135 32 545 32 130 32 593
Cuts at: 2904 2936 3016 3048 4618 4650 4692 4724 1373
Size: 32 80 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1570 593 545 135 130 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 3321 3567 669
Size: 988 281 214 1169 246 3079
Fragments arranged by size:
3079 1169 988 281 246 214
HindIII A'AGCT_T
Cuts at: 748 3153 748
Size: 2405 3572
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 5091 5275 1305
Size: 936 2850 184 2007
Fragments arranged by size:
2850 2007 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3935 4294 4453 518
Size: 648 1287 1482 359 159 2042
Fragments arranged by size:
2042 1482 1287 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2662 3393 4070 5218 5463 1606
Size: 1056 731 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 731 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2260 2554 3113 3335 4880 1132
Size: 97 1031 294 559 222 1545 2229
Fragments arranged by size:
2229 1545 1031 559 294 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2256 4876 2256
Size: 2620 3357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 3127 1108
Size: 1554 465 3958
Fragments arranged by size:
3958 1554 465
PfoI T'CCnGG_A
Cuts at: 3020 3020
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2979 2979
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 3329 830
Size: 2499 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2662 2662
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 2150 3319 1936
Size: 214 1169 4594
Fragments arranged by size:
4594 1169 214
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 3271 3325 4460 5141 5332 826
Size: 219 2226 54 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3137 4081 4982 5244 820
Size: 197 926 249 945 944 901 262 1553
Fragments arranged by size:
1553 945 944 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3113 3335 1132
Size: 1981 222 3774
Fragments arranged by size:
3774 1981 222
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 3125 1517
Size: 1608 4369
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 3126 3416 513
Size: 552 939 231 891 290 3074
Fragments arranged by size:
3074 939 891 552 290 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 3000 3115 4004 4021 4174 4359
Size: 1255 1089 331 115 889 17 153 185
Cuts at: 4359 4804 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1089 889 445 331 185 153
115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 4223 5935
Size: 274 80 33 51 1443 276 1970 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1970 1712 1443 276 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 5937 5969 2996
Size: 32 2909 32 3004
Fragments arranged by size:
3004 2909 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2147 1316
Size: 831 5146
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2659 2011
Size: 648 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI