Restriction map for
pFN21AE2297
(Circular) (Six-base) MAPSORT of: pFN21AE2297.seq Check: 9148 from: 1 to: 9685
pFN21AE2297 9685 bp
With 182 enzymes: SgfI *
November 4, 2010 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3920 4057 4088 3920
Size: 137 31 9517
Fragments arranged by size:
9517 137 31
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7495 278
Size: 53 83 186 936 5959 2468
Fragments arranged by size:
5959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2127 5802 7028 1937
Size: 190 3675 1226 4594
Fragments arranged by size:
4594 3675 1226 190
Acc65I G'GTAC_C
Cuts at: 7006 7006
Size: 9685
AclI AA'CG_TT
Cuts at: 1563 7812 8185 1563
Size: 6249 373 3063
Fragments arranged by size:
6249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2853 2922 3104 3237 4790 5234 5260 5261
Size: 1221 69 182 133 1553 444 26 1
Cuts at: 5261 6400 6407 7748 9132 9400 1632
Size: 1139 7 1341 1384 268 1917
Fragments arranged by size:
1917 1553 1384 1341 1221 1139 444 268
182 133 69 26 7 1
AflII C'TTAA_G
Cuts at: 820 1017 4007 6632 820
Size: 197 2990 2625 3873
Fragments arranged by size:
3873 2990 2625 197
AflIII A'CryG_T
Cuts at: 1203 3818 8584 9561 1203
Size: 2615 4766 977 1327
Fragments arranged by size:
4766 2615 1327 977
AgeI A'CCGG_T
Cuts at: 8516 8516
Size: 9685
AhdI GACnn_n'nnGTC
Cuts at: 2112 3479 8414 2112
Size: 1367 4935 3383
Fragments arranged by size:
4935 3383 1367
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9685
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6519 6551 6519
Size: 32 9653
AlwNI CAG_nnn'CTG
Cuts at: 2327 3833 4669 5500 6333 6904 6978 7428 9000
Size: 1506 836 831 833 571 74 450 1572
Cuts at: 9000 2327
Size: 3012
Fragments arranged by size:
3012 1572 1506 836 833 831 571 450
74
ApaI G_GGCC'C
Cuts at: 2245 6183 2245
Size: 3938 5747
ApaLI G'TGCA_C
Cuts at: 3227 4858 7742 8898 3227
Size: 1631 2884 1156 4014
Fragments arranged by size:
4014 2884 1631 1156
ApoI r'AATT_y
Cuts at: 1493 3749 6994 7220 7372 9481 1493
Size: 2256 3245 226 152 2109 1697
Fragments arranged by size:
3245 2256 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8239 160
Size: 872 7207 1606
Fragments arranged by size:
7207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9685
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 3441 3803 4913 5057 7010 1870
Size: 73 504 994 362 1110 144 1953 4545
Fragments arranged by size:
4545 1953 1110 994 504 362 144 73
BamHI G'GATC_C
Cuts at: 6359 7015 7382 6359
Size: 656 367 8662
Fragments arranged by size:
8662 656 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2581 3079 4326 4529
Size: 325 217 627 272 522 498 1247 203
Cuts at: 4529 5510 7006 8461 618
Size: 981 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 1247 981 627 522 498
325 272 217 203
BanII G_rGCy'C
Cuts at: 1391 2085 2245 2809 2945 3153 3560 3729 3842
Size: 694 160 564 136 208 407 169 113
Cuts at: 3842 4063 4253 4428 5588 5765 6143 6183 6385
Size: 221 190 175 1160 177 378 40 202
Cuts at: 6385 6447 6861 7004 1391
Size: 62 414 143 4072
Fragments arranged by size:
4072 1160 694 564 414 407 378 221
208 202 190 177 175 169 160 143
136 113 62 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2358 3873 4893 5476 6667 928
Size: 971 459 1515 1020 583 1191 3946
Fragments arranged by size:
3946 1515 1191 1020 971 583 459
BbvCI CC'TCA_GC
Cuts at: 2570 6438 2570
Size: 3868 5817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7876 7910 1903
Size: 34 5939 34 3678
Fragments arranged by size:
5939 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7577 8787 7577
Size: 1210 8475
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9685
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2681 4712
Size: 107 122 71 631 605 538 471 2031
Cuts at: 4712 5102 6311 8296 136
Size: 390 1209 1985 1525
Fragments arranged by size:
2031 1985 1525 1209 631 605 538 471
390 122 107 71
BglII A'GATC_T
Cuts at: 2589 2783 9680 2589
Size: 194 6897 2594
Fragments arranged by size:
6897 2594 194
BlpI GC'TnA_GC
Cuts at: 2045 5141 5820 6855 7102 2045
Size: 3096 679 1035 247 4628
Fragments arranged by size:
4628 3096 1035 679 247
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2586 2629 2915 3231 3570 3792 4822
Size: 455 341 43 286 316 339 222 1030
Cuts at: 4822 4862 5523 5832 6183 6604 6760 6766 6845
Size: 40 661 309 351 421 156 6 79
Cuts at: 6845 6915 7746 8902 1790
Size: 70 831 1156 2573
Fragments arranged by size:
2573 1156 1030 831 661 455 421 351
341 339 316 309 286 222 156 79
70 43 40 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2088 2118 2152
Size: 416 53 83 399 24 671 30 34
Cuts at: 2152 2785 3396 5886 7416 8374 442
Size: 633 611 2490 1530 958 1753
Fragments arranged by size:
2490 1753 1530 958 671 633 611 416
399 83 53 34 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 3100 3147 4430 5335 6137 2353
Size: 747 47 1283 905 802 5901
Fragments arranged by size:
5901 1283 905 802 747 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2859 2910 3008 3378 3598 4319 4576
Size: 1238 382 51 98 370 220 721 257
Cuts at: 4576 5195 6132 6443 8345 1239
Size: 619 937 311 1902 2579
Fragments arranged by size:
2579 1902 1238 937 721 619 382 370
311 257 220 98 51
Bpu10I CC'TnA_GC
Cuts at: 2246 2570 2630 3494 5582 6438 2246
Size: 324 60 864 2088 856 5493
Fragments arranged by size:
5493 2088 864 856 324 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5237 7810 8675 8973 5237
Size: 2573 865 298 5949
Fragments arranged by size:
5949 2573 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8348 882
Size: 617 6849 2219
Fragments arranged by size:
6849 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7476 493
Size: 711 6272 2702
Fragments arranged by size:
6272 2702 711
BsaBI GATnn'nnATC
Cuts at: 4364 9679 4364
Size: 5315 4370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5050 5643 7492 7874
Size: 53 83 186 936 3517 593 1849 382
Cuts at: 7874 8509 275
Size: 635 1451
Fragments arranged by size:
3517 1849 1451 936 635 593 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4209 4440 4488 4650 5149 8118 8516 8790 8937
Size: 231 48 162 499 2969 398 274 147
Cuts at: 8937 9348 4209
Size: 411 4546
Fragments arranged by size:
4546 2969 499 411 398 274 231 162
147 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2981 3011 3221 3251 3351
Size: 30 165 30 1578 30 210 30 100
Cuts at: 3351 3381 4169 4199 5264 5294 6389 6419 6497
Size: 30 788 30 1065 30 1095 30 78
Cuts at: 6497 6500 6527 6530 1178
Size: 3 27 3 4333
Fragments arranged by size:
4333 1578 1095 1065 788 210 165 100
78 30 30 30 30 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2558 2689 3941 4193 4316 4994 5649
Size: 1324 48 131 1252 252 123 678 655
Cuts at: 5649 6302 6410 6440 6455 6485 6509 6518 6521
Size: 653 108 30 15 30 24 9 3
Cuts at: 6521 6788 1186
Size: 267 4083
Fragments arranged by size:
4083 1324 1252 678 655 653 267 252
131 123 108 48 30 30 24 15
9 3
BseYI C'CCAG_C
Cuts at: 2101 2161 2294 2545 3502 3656 3826 5229 5326
Size: 60 133 251 957 154 170 1403 97
Cuts at: 5326 5399 5621 5964 6176 6182 6208 6449 6571
Size: 73 222 343 212 6 26 241 122
Cuts at: 6571 6787 6861 8888 9384 2101
Size: 216 74 2027 496 2402
Fragments arranged by size:
2402 2027 1403 957 496 343 251 241
222 216 212 170 154 133 122 97
74 73 60 26 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3539 4855 5555 6599 1163
Size: 573 1803 1316 700 1044 4249
Fragments arranged by size:
4249 1803 1316 1044 700 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4155 7135 7896 8924 1540
Size: 275 186 2154 2980 761 1028 2301
Fragments arranged by size:
2980 2301 2154 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2841 2945 3231 3729 3903 4253 4428 4650 4862
Size: 104 286 498 174 350 175 222 212
Cuts at: 4862 5025 5147 5765 7746 7831 8902 2841
Size: 163 122 618 1981 85 1071 3624
Fragments arranged by size:
3624 1981 1071 618 498 350 286 222
212 175 174 163 122 104 85
BsmI GAATG_Cn'
Cuts at: 2438 4389 4996 7196 7289 2438
Size: 1951 607 2200 93 4834
Fragments arranged by size:
4834 2200 1951 607 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5829 5829
Size: 9685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2245 2586 2629 2809 2841 2915
Size: 399 295 160 341 43 180 32 74
Cuts at: 2915 2945 3153 3231 3560 3570 3729 3792 3842
Size: 30 208 78 329 10 159 63 50
Cuts at: 3842 3903 4063 4253 4428 4650 4822 4862 5025
Size: 61 160 190 175 222 172 40 163
Cuts at: 5025 5147 5523 5588 5765 5832 6143 6183 6385
Size: 122 376 65 177 67 311 40 202
Cuts at: 6385 6447 6604 6760 6766 6845 6861 6915 7004
Size: 62 157 156 6 79 16 54 89
Cuts at: 7004 7746 7831 8902 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 341 329 311
295 222 208 202 190 180 177 175
172 163 160 160 159 157 156 122
89 85 79 78 74 67 65 63
62 61 54 50 43 40 40 32
30 16 10 6
BspEI T'CCGG_A
Cuts at: 4209 4440 4650 5149 9348 4209
Size: 231 210 499 4199 4546
Fragments arranged by size:
4546 4199 499 231 210
BspHI T'CATG_A
Cuts at: 7574 9304 7574
Size: 1730 7955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3920 4057 4088 4502 7040 844
Size: 3076 137 31 414 2538 3489
Fragments arranged by size:
3489 3076 2538 414 137 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 4183 6461 7572 1394
Size: 429 677 1683 2278 1111 3507
Fragments arranged by size:
3507 2278 1683 1111 677 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2634 2670 4266 8180 8354 65
Size: 1183 1386 36 1596 3914 174 1396
Fragments arranged by size:
3914 1596 1396 1386 1183 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2537 3532 5337 6433 8329 8516
Size: 110 252 475 995 1805 1096 1896 187
Cuts at: 8516 1700
Size: 2869
Fragments arranged by size:
2869 1896 1805 1096 995 475 252 187
110
BsrGI T'GTAC_A
Cuts at: 96 4692 4944 96
Size: 4596 252 4837
Fragments arranged by size:
4837 4596 252
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9685
BssSI C'ACGA_G
Cuts at: 7745 8757 7745
Size: 1012 8673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9685
BstBI TT'CG_AA
Cuts at: 8489 8489
Size: 9685
BstEII G'GTnAC_C
Cuts at: 1184 4729 6928 1184
Size: 3545 2199 3941
Fragments arranged by size:
3941 3545 2199
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 6037 6322 2101
Size: 3936 285 5464
Fragments arranged by size:
5464 3936 285
BstYI r'GATC_y
Cuts at: 1972 2589 2783 4139 5780 6359 7015 7382 7767
Size: 617 194 1356 1641 579 656 367 385
Cuts at: 7767 7784 9236 9322 9334 9680 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1641 1452 1356 656 617 579 385
367 346 194 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2053 2328 2754 3042 4710 2053
Size: 275 426 288 1668 7028
Fragments arranged by size:
7028 1668 426 288 275
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2976 3302 3366 4149 4255
Size: 552 939 22 950 326 64 783 106
Cuts at: 4255 4863 6134 6200 6564 6577 6709 513
Size: 608 1271 66 364 13 132 3489
Fragments arranged by size:
3489 1271 950 939 783 608 552 364
326 132 106 66 64 22 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 487
Size: 683 56 1210 7736
Fragments arranged by size:
7736 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 2363 4342 5539 7201 7995 8015 2363
Size: 1979 1197 1662 794 20 4033
Fragments arranged by size:
4033 1979 1662 1197 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4507 4542 529
Size: 35 3943 35 5672
Fragments arranged by size:
5672 3943 35 35
DraI TTT'AAA
Cuts at: 6989 6989
Size: 9685
DraIII CAC_nnn'GTG
Cuts at: 5731 5731
Size: 9685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 6926 8692 809
Size: 507 4547 1063 1766 1802
Fragments arranged by size:
4547 1802 1766 1063 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2318 4671
Size: 54 1425 50 275 18 387 101 2353
Cuts at: 4671 4953 6061 6561 7132 8021 9650 8
Size: 282 1108 500 571 889 1629 43
Fragments arranged by size:
2353 1629 1425 1108 889 571 500 387
282 275 101 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7132 1537
Size: 275 5320 4090
Fragments arranged by size:
5320 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2829 3091 3218 3464 3702 3717 4649 6495
Size: 1476 262 127 246 238 15 932 1846
Cuts at: 6495 7615 1353
Size: 1120 3423
Fragments arranged by size:
3423 1846 1476 1120 932 262 246 238
127 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4601 6764 8272 8646 8792 387
Size: 1064 138 3012 2163 1508 374 146 1280
Fragments arranged by size:
3012 2163 1508 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2943 3727 4251 4426 5763 2943
Size: 784 524 175 1337 6865
Fragments arranged by size:
6865 1337 784 524 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2853 2859 2910 2922 3008 3104
Size: 393 845 376 6 51 12 86 96
Cuts at: 3104 3237 3378 3598 4319 4576 4790 5195 5234
Size: 133 141 220 721 257 214 405 39
Cuts at: 5234 5260 5261 6132 6400 6407 6443 7748 8345
Size: 26 1 871 268 7 36 1305 597
Cuts at: 8345 9132 9400 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1305 871 845 787 721 597 405
393 376 268 268 257 220 214 141
133 96 86 51 39 36 26 12
7 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 3221 4418 4880 6425 2753
Size: 468 1197 462 1545 6013
Fragments arranged by size:
6013 1545 1197 468 462
EcoO109I rG'GnC_Cy
Cuts at: 2114 2241 2242 2312 2324 2516 2605 2683 3046
Size: 127 1 70 12 192 89 78 363
Cuts at: 3046 3362 3438 3498 3651 3799 4666 5170 5504
Size: 316 76 60 153 148 867 504 334
Cuts at: 5504 5687 5713 5740 5888 6330 6409 2114
Size: 183 26 27 148 442 79 5390
Fragments arranged by size:
5390 867 504 442 363 334 316 192
183 153 148 148 127 89 79 78
76 70 60 27 26 12 1
EcoRI G'AATT_C
Cuts at: 6994 7372 6994
Size: 378 9307
FseI GG_CCGG'CC
Cuts at: 2541 2541
Size: 9685
FspI TGC'GCA
Cuts at: 8191 8191
Size: 9685
HaeII r_GCGC'y
Cuts at: 1678 2052 3009 6209 8832 1678
Size: 374 957 3200 2623 2531
Fragments arranged by size:
3200 2623 2531 957 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2747 2779 2828
Size: 32 135 32 526 32 617 32 49
Cuts at: 2828 2860 3465 3497 4461 4493 6212 6244 6500
Size: 32 605 32 964 32 1719 32 256
Cuts at: 6500 6532 8326 8358 8400 8432 1373
Size: 32 1794 32 42 32 2626
Fragments arranged by size:
2626 1794 1719 964 617 605 526 256
135 49 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7029 7275 669
Size: 988 281 5091 246 3079
Fragments arranged by size:
5091 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9685
HpaI GTT'AAC
Cuts at: 7275 7275
Size: 9685
KpnI G_GTAC'C
Cuts at: 7010 7010
Size: 9685
MfeI C'AATT_G
Cuts at: 7284 7284
Size: 9685
MluI A'CGCG_T
Cuts at: 9561 9561
Size: 9685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3496 5071 5175 5370 5790 6498 8799 8983
Size: 2191 1575 104 195 420 708 2301 184
Cuts at: 8983 1305
Size: 2007
Fragments arranged by size:
2301 2191 2007 1575 708 420 195 184
104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4673 4955 6063 10
Size: 54 1425 343 2841 282 1108 3632
Fragments arranged by size:
3632 2841 1425 1108 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5018 7643 8002 8161 518
Size: 648 3852 2625 359 159 2042
Fragments arranged by size:
3852 2625 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3356 4257 4927 5007 5083 5089 5295
Size: 481 1269 901 670 80 76 6 206
Cuts at: 5295 5809 5964 6033 6322 6648 6745 6978 7101
Size: 514 155 69 289 326 97 233 123
Cuts at: 7101 7778 8926 9171 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1269 1148 901 677 670 514 481
326 289 245 233 206 155 123 97
80 76 69 6
NaeI GCC'GGC
Cuts at: 2539 5339 6435 2539
Size: 2800 1096 5789
Fragments arranged by size:
5789 2800 1096
NcoI C'CATG_G
Cuts at: 513 1065 2004 2026 2976 513
Size: 552 939 22 950 7222
Fragments arranged by size:
7222 950 939 552 22
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9685
NgoMIV G'CCGG_C
Cuts at: 2537 5337 6433 2537
Size: 2800 1096 5789
Fragments arranged by size:
5789 2800 1096
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9685
NotI GC'GGCC_GC
Cuts at: 7132 7132
Size: 9685
NspI r_CATG'y
Cuts at: 1132 1229 3822 4564 4934 5932 7043 8588 1132
Size: 97 2593 742 370 998 1111 1545 2229
Fragments arranged by size:
2593 2229 1545 1111 998 742 370 97
PasI CC'CwG_GG
Cuts at: 1348 2513 3389 6355 1348
Size: 1165 876 2966 4678
Fragments arranged by size:
4678 2966 1165 876
PciI A'CATG_T
Cuts at: 3818 8584 3818
Size: 4766 4919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 4669 6715 1108
Size: 1089 2472 2046 4078
Fragments arranged by size:
4078 2472 2046 1089
PfoI T'CCnGG_A
Cuts at: 3483 4164 4553 4662 6537 3483
Size: 681 389 109 1875 6631
Fragments arranged by size:
6631 1875 681 389 109
PmeI GTTT'AAAC
Cuts at: 6989 6989
Size: 9685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6149 6181 7754 7786 9267 9299 6149
Size: 32 1573 32 1481 32 6535
Fragments arranged by size:
6535 1573 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2114 2312 3362 3498 3651 3799 4666 5504 5740
Size: 198 1050 136 153 148 867 838 236
Cuts at: 5740 5888 6409 2114
Size: 148 521 5390
Fragments arranged by size:
5390 1050 867 838 521 236 198 153
148 148 136
PshAI GACnn'nnGTC
Cuts at: 1613 4758 5411 1613
Size: 3145 653 5887
Fragments arranged by size:
5887 3145 653
PsiI TTA'TAA
Cuts at: 7255 7255
Size: 9685
PspOMI G'GGCC_C
Cuts at: 2241 6179 2241
Size: 3938 5747
PspXI vC'TCGA_Gb
Cuts at: 2447 2447
Size: 9685
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5354 5386 5354
Size: 32 9653
PstI C_TGCA'G
Cuts at: 830 2370 3186 7037 830
Size: 1540 816 3851 3478
Fragments arranged by size:
3851 3478 1540 816
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9685
PvuII CAG'CTG
Cuts at: 3356 4927 5089 5964 6033 6322 6745 3356
Size: 1571 162 875 69 289 423 6296
Fragments arranged by size:
6296 1571 875 423 289 162 69
SacI G_AGCT'C
Cuts at: 2945 3729 4253 4428 5765 2945
Size: 784 524 175 1337 6865
Fragments arranged by size:
6865 1337 784 524 175
SacII CC_GC'GG
Cuts at: 4258 4258
Size: 9685
SalI G'TCGA_C
Cuts at: 1936 7027 1936
Size: 5091 4594
SanDI GG'GwC_CC
Cuts at: 2312 5740 6409 2312
Size: 3428 669 5588
Fragments arranged by size:
5588 3428 669
SapI GCTCTTCn'nnn_
Cuts at: 2829 3717 2829
Size: 888 8797
SbfI CC_TGCA'GG
Cuts at: 7037 7037
Size: 9685
ScaI AGT'ACT
Cuts at: 5912 7933 9645 5912
Size: 2021 1712 5952
Fragments arranged by size:
5952 2021 1712
SexAI A'CCwGG_T
Cuts at: 4725 4725
Size: 9685
SfcI C'TryA_G
Cuts at: 826 1045 2366 3182 6970 7033 8168 8849 9040
Size: 219 1321 816 3788 63 1135 681 191
Cuts at: 9040 826
Size: 1471
Fragments arranged by size:
3788 1471 1321 1135 816 681 219 191
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 4712 4712
Size: 9685
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9685
SmaI CCC'GGG
Cuts at: 1872 3443 7012 1872
Size: 1571 3569 4545
Fragments arranged by size:
4545 3569 1571
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 4007 5252 6632 7789 8690
Size: 197 926 504 1560 1245 1380 1157 901
Cuts at: 8690 8952 820
Size: 262 1553
Fragments arranged by size:
1560 1553 1380 1245 1157 926 901 504
262 197
SnaBI TAC'GTA
Cuts at: 493 7476 493
Size: 6983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9685
SphI G_CATG'C
Cuts at: 1132 7043 1132
Size: 5911 3774
SspI AAT'ATT
Cuts at: 5 52 7609 5
Size: 47 7557 2081
Fragments arranged by size:
7557 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 4715 5205 5238 1517
Size: 1242 1956 490 33 5964
Fragments arranged by size:
5964 1956 1242 490 33
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2117 2640 2976 3063 3324
Size: 552 939 22 91 523 336 87 261
Cuts at: 3324 3954 4504 4881 5691 5709 6171 7124 513
Size: 630 550 377 810 18 462 953 3074
Fragments arranged by size:
3074 953 939 810 630 552 550 523
462 377 336 261 91 87 22 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 3173 3337 3964 3991 4039 4267
Size: 1255 842 751 164 627 27 48 228
Cuts at: 4267 4845 5945 7712 7729 7882 8067 8512 325
Size: 578 1100 1767 17 153 185 445 1498
Fragments arranged by size:
1767 1498 1255 1100 842 751 627 578
445 228 185 164 153 48 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3115 4563 4692
Size: 274 80 33 51 1443 1138 1448 129
Cuts at: 4692 4944 5289 5370 5910 7931 9643 96
Size: 252 345 81 540 2021 1712 138
Fragments arranged by size:
2021 1712 1448 1443 1138 540 345 274
252 138 129 81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3351 3383 5264 5296 5717 5749 5935
Size: 32 98 32 1881 32 421 32 186
Cuts at: 5935 5967 6389 6421 6497 6529 9645 9677 3221
Size: 32 422 32 76 32 3116 32 3229
Fragments arranged by size:
3229 3116 1881 422 421 186 98 76
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2094 5892 1316
Size: 778 3798 5109
Fragments arranged by size:
5109 3798 778
XbaI T'CTAG_A
Cuts at: 7021 7021
Size: 9685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 3070 4036 6056 6712 2983
Size: 87 966 2020 656 5956
Fragments arranged by size:
5956 2020 966 656 87
XhoI C'TCGA_G
Cuts at: 1943 2447 1943
Size: 504 9181
XmaI C'CCGG_G
Cuts at: 1870 3441 7010 1870
Size: 1571 3569 4545
Fragments arranged by size:
4545 3569 1571
XmnI GAAnn'nnTTC
Cuts at: 4658 5273 7814 4658
Size: 615 2541 6529
Fragments arranged by size:
6529 2541 615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7493 276
Size: 53 83 186 936 5959 2468
Fragments arranged by size:
5959 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PmlI RsrII SfoI SrfI SwaI