Restriction map for
pFN21AE2296
(Circular) (Six-base) MAPSORT of: pFN21AE2296.seq Check: 9750 from: 1 to: 8200
pFN21AE2296 8200 bp
With 182 enzymes: SgfI *
November 4, 2010 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5050 5050
Size: 8200
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6010 278
Size: 53 83 186 936 4474 2468
Fragments arranged by size:
4474 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5543 1937
Size: 3606 4594
Acc65I G'GTAC_C
Cuts at: 5521 5521
Size: 8200
AclI AA'CG_TT
Cuts at: 1563 6327 6700 1563
Size: 4764 373 3063
Fragments arranged by size:
4764 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2525 3246 3647 3758 4110 4668 5120
Size: 677 216 721 401 111 352 558 452
Cuts at: 5120 5250 5457 6263 7647 7915 1632
Size: 130 207 806 1384 268 1917
Fragments arranged by size:
1917 1384 806 721 677 558 452 401
352 268 216 207 130 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8003
AflIII A'CryG_T
Cuts at: 1203 3406 7099 8076 1203
Size: 2203 3693 977 1327
Fragments arranged by size:
3693 2203 1327 977
AgeI A'CCGG_T
Cuts at: 7031 7031
Size: 8200
AhdI GACnn_n'nnGTC
Cuts at: 6929 6929
Size: 8200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3098 1184
Size: 1914 6286
AlwNI CAG_nnn'CTG
Cuts at: 2548 2645 4030 4133 5098 5943 7515 2548
Size: 97 1385 103 965 845 1572 3233
Fragments arranged by size:
3233 1572 1385 965 845 103 97
ApaLI G'TGCA_C
Cuts at: 3983 6257 7413 3983
Size: 2274 1156 4770
Fragments arranged by size:
4770 2274 1156
ApoI r'AATT_y
Cuts at: 1493 3056 3826 4450 5356 5464 5509 5735 5887
Size: 1563 770 624 906 108 45 226 152
Cuts at: 5887 7996 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1563 906 770 624 226 152
108 45
AseI AT'TA_AT
Cuts at: 160 1032 6754 160
Size: 872 5722 1606
Fragments arranged by size:
5722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8200
AvaI C'yCGr_G
Cuts at: 1870 1943 5525 1870
Size: 73 3582 4545
Fragments arranged by size:
4545 3582 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3887 3920 5358 5391 3887
Size: 33 1438 33 6696
Fragments arranged by size:
6696 1438 33 33
BamHI G'GATC_C
Cuts at: 5530 5897 5530
Size: 367 7833
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 5521 6976 618
Size: 325 217 627 1586 2148 1455 1842
Fragments arranged by size:
2148 1842 1586 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2137 2322 3849 5519 1391
Size: 746 185 1527 1670 4072
Fragments arranged by size:
4072 1670 1527 746 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7229
BbvCI CC'TCA_GC
Cuts at: 3252 3252
Size: 8200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6391 6425 1903
Size: 34 4454 34 3678
Fragments arranged by size:
4454 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5359 6092 7302 5359
Size: 733 1210 6257
Fragments arranged by size:
6257 1210 733
BclI T'GATC_A
Cuts at: 1548 4306 1548
Size: 2758 5442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3844 6811 136
Size: 107 122 71 631 605 2172 2967 1525
Fragments arranged by size:
2967 2172 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3655 4633 8195 3655
Size: 978 3562 3660
Fragments arranged by size:
3660 3562 978
BlpI GC'TnA_GC
Cuts at: 4749 5617 4749
Size: 868 7332
Bme1580I G_kGCm'C
Cuts at: 1790 3666 3987 6261 7417 1790
Size: 1876 321 2274 1156 2573
Fragments arranged by size:
2573 2274 1876 1156 321
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4088 5931 6889
Size: 416 53 83 399 24 2671 1843 958
Cuts at: 6889 442
Size: 1753
Fragments arranged by size:
2671 1843 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3044 1056
Size: 1988 6212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 3905 3941 4259 4442 6860 1239
Size: 851 1815 36 318 183 2418 2579
Fragments arranged by size:
2579 2418 1815 851 318 183 36
Bpu10I CC'TnA_GC
Cuts at: 3252 3252
Size: 8200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 6325 7190 7488 3524
Size: 2801 865 298 4236
Fragments arranged by size:
4236 2801 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5075 6863 882
Size: 617 3576 1788 2219
Fragments arranged by size:
3576 2219 1788 617
BsaAI yAC'GTr
Cuts at: 493 1204 5991 493
Size: 711 4787 2702
Fragments arranged by size:
4787 2702 711
BsaBI GATnn'nnATC
Cuts at: 8194 8194
Size: 8200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6007 6389 7024 275
Size: 53 83 186 936 4474 382 635 1451
Fragments arranged by size:
4474 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6633 7031 7305 7452 7863 6633
Size: 398 274 147 411 6970
Fragments arranged by size:
6970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3878 3908 4655 4685 1178
Size: 30 165 30 2475 30 747 30 4693
Fragments arranged by size:
4693 2475 747 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3222 3225 1186
Size: 2036 3 6161
Fragments arranged by size:
6161 2036 3
BseYI C'CCAG_C
Cuts at: 5239 7403 7899 5239
Size: 2164 496 5540
Fragments arranged by size:
5540 2164 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 4043 5081 1163
Size: 573 1014 1293 1038 4282
Fragments arranged by size:
4282 1293 1038 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5650 6411 7439 1540
Size: 275 186 3649 761 1028 2301
Fragments arranged by size:
3649 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2137 3891 3987 6261 6346 7417 2137
Size: 1754 96 2274 85 1071 2920
Fragments arranged by size:
2920 2274 1754 1071 96 85
BsmI GAATG_Cn'
Cuts at: 5711 5804 5711
Size: 93 8107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2322 3666 3849 3891 3987 5519
Size: 399 347 185 1344 183 42 96 1532
Cuts at: 5519 6261 6346 7417 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1532 1344 1071 742 399 347 185
183 96 85 42
BspEI T'CCGG_A
Cuts at: 7863 7863
Size: 8200
BspHI T'CATG_A
Cuts at: 6089 7819 6089
Size: 1730 6470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5050 5555 844
Size: 4206 505 3489
Fragments arranged by size:
4206 3489 505
BsrBI CCG'CTC
Cuts at: 1394 1823 6087 1394
Size: 429 4264 3507
Fragments arranged by size:
4264 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 2937 6695 6869 65
Size: 1183 1473 216 3758 174 1396
Fragments arranged by size:
3758 1473 1396 1183 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6844 7031 1700
Size: 110 5034 187 2869
Fragments arranged by size:
5034 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8200
BssSI C'ACGA_G
Cuts at: 3651 6260 7272 3651
Size: 2609 1012 4579
Fragments arranged by size:
4579 2609 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4597 794
Size: 3803 4397
BstBI TT'CG_AA
Cuts at: 7004 7004
Size: 8200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8200
BstYI r'GATC_y
Cuts at: 1972 3655 4633 5530 5897 6282 6299 7751 7837
Size: 1683 978 897 367 385 17 1452 86
Cuts at: 7837 7849 8195 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1683 1452 978 897 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2815 3369 2815
Size: 554 7646
BtgI C'CryG_G
Cuts at: 513 1065 2004 2655 5085 513
Size: 552 939 651 2430 3628
Fragments arranged by size:
3628 2430 939 651 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2790 487
Size: 683 56 1564 5897
Fragments arranged by size:
5897 1564 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 2524 2622 2880 3574 3758 5211 5716 6510
Size: 404 98 258 694 184 1453 505 794
Cuts at: 6510 6530 2120
Size: 20 3790
Fragments arranged by size:
3790 1453 794 694 505 404 258 184
98 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4932 4967 529
Size: 35 4368 35 3762
Fragments arranged by size:
4368 3762 35 35
DraI TTT'AAA
Cuts at: 3117 5504 3117
Size: 2387 5813
DraIII CAC_nnn'GTG
Cuts at: 2163 2672 3982 4034 2163
Size: 509 1310 52 6329
Fragments arranged by size:
6329 1310 509 52
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7207 809
Size: 507 5891 1802
Fragments arranged by size:
5891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4333 5647 6536
Size: 54 1425 50 275 18 2503 1314 889
Cuts at: 6536 8165 8
Size: 1629 43
Fragments arranged by size:
2503 1629 1425 1314 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5647 1537
Size: 275 3835 4090
Fragments arranged by size:
4090 3835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2656 3243 6130 1353
Size: 1303 587 2887 3423
Fragments arranged by size:
3423 2887 1303 587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6787 7161 7307 387
Size: 1064 138 5198 374 146 1280
Fragments arranged by size:
5198 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2135 2135
Size: 8200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2309 2525 3246 3647 3758 3905
Size: 393 458 219 216 721 401 111 147
Cuts at: 3905 3941 4110 4259 4442 4668 5120 5250 5457
Size: 36 169 149 183 226 452 130 207
Cuts at: 5457 6263 6860 7647 7915 1239
Size: 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 721 597 458 452 401
393 268 226 219 216 207 183 169
149 147 130 111 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3264 3264
Size: 8200
EcoO109I rG'GnC_Cy
Cuts at: 3709 3709
Size: 8200
EcoRI G'AATT_C
Cuts at: 3056 5509 5887 3056
Size: 2453 378 5369
Fragments arranged by size:
5369 2453 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3193 3876 4655 3193
Size: 683 779 6738
Fragments arranged by size:
6738 779 683
FspI TGC'GCA
Cuts at: 4740 6706 4740
Size: 1966 6234
HaeII r_GCGC'y
Cuts at: 1678 7347 1678
Size: 5669 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2122 2154 3379
Size: 32 135 32 502 32 16 32 1225
Cuts at: 3379 3411 3878 3910 4198 4230 4270 4302 4537
Size: 32 467 32 288 32 40 32 235
Cuts at: 4537 4569 4655 4687 5381 5413 6841 6873 6915
Size: 32 86 32 694 32 1428 32 42
Cuts at: 6915 6947 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 1225 694 502 467 288 235
135 86 42 40 32 32 32 32
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2417 4730 5544 5790 669
Size: 988 281 479 2313 814 246 3079
Fragments arranged by size:
3079 2313 988 814 479 281 246
HindIII A'AGCT_T
Cuts at: 748 3858 4680 748
Size: 3110 822 4268
Fragments arranged by size:
4268 3110 822
HpaI GTT'AAC
Cuts at: 5790 5790
Size: 8200
KpnI G_GTAC'C
Cuts at: 5525 5525
Size: 8200
MfeI C'AATT_G
Cuts at: 3212 5799 3212
Size: 2587 5613
MluI A'CGCG_T
Cuts at: 8076 8076
Size: 8200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3855 7314 7498 1305
Size: 2550 3459 184 2007
Fragments arranged by size:
3459 2550 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4335 10
Size: 54 1425 343 2503 3875
Fragments arranged by size:
3875 2503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 2726 2751 3409 4773 4779 6158
Size: 648 1501 59 25 658 1364 6 1379
Cuts at: 6158 6517 6676 518
Size: 359 159 2042
Fragments arranged by size:
2042 1501 1379 1364 658 648 359 159
59 25 6
MspA1I CmG'CkG
Cuts at: 1606 2870 3883 5294 5616 6293 7441 7686 1606
Size: 1264 1013 1411 322 677 1148 245 2120
Fragments arranged by size:
2120 1411 1264 1148 1013 677 322 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5085 513
Size: 552 939 3081 3628
Fragments arranged by size:
3628 3081 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8200
NheI G'CTAG_C
Cuts at: 1052 3040 1052
Size: 1988 6212
NotI GC'GGCC_GC
Cuts at: 5647 5647
Size: 8200
NspI r_CATG'y
Cuts at: 1132 1229 3410 3838 4488 4600 4853 4976 5558
Size: 97 2181 428 650 112 253 123 582
Cuts at: 5558 7103 1132
Size: 1545 2229
Fragments arranged by size:
2229 2181 1545 650 582 428 253 123
112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8200
PciI A'CATG_T
Cuts at: 3406 7099 3406
Size: 3693 4507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3560 1108
Size: 2452 5748
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 8200
PmeI GTTT'AAAC
Cuts at: 5504 5504
Size: 8200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2295 2327 6269 6301 7782 7814 2295
Size: 32 3942 32 1481 32 2681
Fragments arranged by size:
3942 2681 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3526 1613
Size: 1913 6287
PsiI TTA'TAA
Cuts at: 5770 5770
Size: 8200
PstI C_TGCA'G
Cuts at: 830 2519 2875 3013 3484 5221 5552 830
Size: 1689 356 138 471 1737 331 3478
Fragments arranged by size:
3478 1737 1689 471 356 331 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8200
PvuII CAG'CTG
Cuts at: 2870 3883 5294 2870
Size: 1013 1411 5776
Fragments arranged by size:
5776 1411 1013
SacI G_AGCT'C
Cuts at: 2137 2137
Size: 8200
SalI G'TCGA_C
Cuts at: 1936 5542 1936
Size: 3606 4594
SbfI CC_TGCA'GG
Cuts at: 5552 5552
Size: 8200
ScaI AGT'ACT
Cuts at: 6448 8160 6448
Size: 1712 6488
SexAI A'CCwGG_T
Cuts at: 3978 3978
Size: 8200
SfcI C'TryA_G
Cuts at: 826 1045 2463 2515 2637 2871 3009 3480 3578
Size: 219 1418 52 122 234 138 471 98
Cuts at: 3578 4691 5217 5548 6683 7364 7555 826
Size: 1113 526 331 1135 681 191 1471
Fragments arranged by size:
1471 1418 1135 1113 681 526 471 331
234 219 191 138 122 98 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8200
SmaI CCC'GGG
Cuts at: 1872 5527 1872
Size: 3655 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3539 6304 7205 7467 820
Size: 197 926 1596 2765 901 262 1553
Fragments arranged by size:
2765 1596 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5991 493
Size: 5498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8200
SphI G_CATG'C
Cuts at: 1132 4488 4853 5558 1132
Size: 3356 365 705 3774
Fragments arranged by size:
3774 3356 705 365
SspI AAT'ATT
Cuts at: 5 52 2702 4604 6124 5
Size: 47 2650 1902 1520 2081
Fragments arranged by size:
2650 2081 1902 1520 47
StuI AGG'CCT
Cuts at: 1517 2888 3442 1517
Size: 1371 554 6275
Fragments arranged by size:
6275 1371 554
StyI C'CwwG_G
Cuts at: 513 1065 2004 3851 3934 5085 5639 513
Size: 552 939 1847 83 1151 554 3074
Fragments arranged by size:
3074 1847 1151 939 554 552 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2817 3881 6227 6244 6397 6582 7027
Size: 1255 1237 1064 2346 17 153 185 445
Cuts at: 7027 325
Size: 1498
Fragments arranged by size:
2346 1498 1255 1237 1064 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4429 5429 6446
Size: 274 80 33 51 1443 2452 1000 1017
Cuts at: 6446 8158 96
Size: 1712 138
Fragments arranged by size:
2452 1712 1443 1017 1000 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8160 8192 8160
Size: 32 8168
Tth111I GACn'n_nGTC
Cuts at: 1316 2046 1316
Size: 730 7470
XbaI T'CTAG_A
Cuts at: 5536 5536
Size: 8200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 4721 2357
Size: 2364 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8200
XmaI C'CCGG_G
Cuts at: 1870 5525 1870
Size: 3655 4545
XmnI GAAnn'nnTTC
Cuts at: 4356 6329 4356
Size: 1973 6227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6008 276
Size: 53 83 186 936 4474 2468
Fragments arranged by size:
4474 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI