Restriction map for
pFN21AE2293
(Circular) (Six-base) MAPSORT of: pFN21AE2293.seq Check: 9052 from: 1 to: 5323
pFN21AE2293 5323 bp
With 182 enzymes: SgfI *
March 28, 2012 12:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3386 4770 5038 1632
Size: 1754 1384 268 1917
Fragments arranged by size:
1917 1754 1384 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 5323
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaI G_GGCC'C
Cuts at: 2016 2016
Size: 5323
ApaLI G'TGCA_C
Cuts at: 2467 3380 4536 2467
Size: 913 1156 3254
Fragments arranged by size:
3254 1156 913
ApoI r'AATT_y
Cuts at: 1493 2632 2858 3010 5119 1493
Size: 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 226 152
AscI GG'CGCG_CC
Cuts at: 2171 2171
Size: 5323
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2648 1870
Size: 73 72 633 4545
Fragments arranged by size:
4545 633 73 72
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2087 2219 2644 4099
Size: 325 217 627 278 22 132 425 1455
Cuts at: 4099 618
Size: 1842
Fragments arranged by size:
1842 1455 627 425 325 278 217 132
22
BanII G_rGCy'C
Cuts at: 1391 2016 2358 2642 1391
Size: 625 342 284 4072
Fragments arranged by size:
4072 625 342 284
BbeI G_GCGC'C
Cuts at: 2069 2091 2223 2069
Size: 22 132 5169
Fragments arranged by size:
5169 132 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2567 928
Size: 971 668 3684
Fragments arranged by size:
3684 971 668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2521 2555 3514 3548 1903
Size: 34 584 34 959 34 3678
Fragments arranged by size:
3678 959 584 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2085 2316
Size: 107 122 71 631 605 391 22 231
Cuts at: 2316 3934 136
Size: 1618 1525
Fragments arranged by size:
1618 1525 631 605 391 231 122 107
71 22
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2740 2740
Size: 5323
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2471 3384 4540 1790
Size: 226 455 913 1156 2573
Fragments arranged by size:
2573 1156 913 455 226
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2153 2276 2372 3983 1239
Size: 902 12 123 96 1611 2579
Fragments arranged by size:
2579 1611 902 123 96 12
Bpu10I CC'TnA_GC
Cuts at: 2546 2546
Size: 5323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 4313 4611 3448
Size: 865 298 4160
Fragments arranged by size:
4160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3114 493
Size: 711 1910 2702
Fragments arranged by size:
2702 1910 711
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2088 2220 3130
Size: 53 83 186 936 533 22 132 910
Cuts at: 3130 3512 4147 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 910 635 533 382 186 132
83 53 22
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5098
Fragments arranged by size:
5098 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5323
BseYI C'CCAG_C
Cuts at: 2142 2236 4526 5022 2142
Size: 94 2290 496 2443
Fragments arranged by size:
2443 2290 496 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 1163
Size: 573 352 4398
Fragments arranged by size:
4398 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2208 2311 2773 3534 4562
Size: 275 186 24 183 103 462 761 1028
Cuts at: 4562 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 462 275 186 183 103
24
BsiHKAI G_wGCw'C
Cuts at: 2358 2471 3384 3469 4540 2358
Size: 113 913 85 1071 3141
Fragments arranged by size:
3141 1071 913 113 85
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2358 2471 2642 3384 3469 4540
Size: 399 226 342 113 171 742 85 1071
Cuts at: 4540 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 342 226 171 113
85
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 2678 844
Size: 1397 437 3489
Fragments arranged by size:
3489 1397 437
BsrBI CCG'CTC
Cuts at: 1394 1823 2217 2371 3210 1394
Size: 429 394 154 839 3507
Fragments arranged by size:
3507 839 429 394 154
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 2222 2403 3967 4154 1700
Size: 110 369 43 181 1564 187 2869
Fragments arranged by size:
2869 1564 369 187 181 110 43
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 2171 2322 1925
Size: 246 151 4926
Fragments arranged by size:
4926 246 151
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2102 794
Size: 1308 4015
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 2599 1184
Size: 1415 3908
BstYI r'GATC_y
Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318
Size: 681 367 385 17 1452 86 12 346
Cuts at: 5318 1972
Size: 1977
Fragments arranged by size:
1977 1452 681 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2364 513
Size: 552 960 339 3472
Fragments arranged by size:
3472 960 552 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2524 487
Size: 683 56 1298 3286
Fragments arranged by size:
3286 1298 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2839 3633 3653 2100
Size: 739 794 20 3770
Fragments arranged by size:
3770 794 739 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2205 2308
Size: 54 1425 50 275 18 192 183 103
Cuts at: 2308 2397 2415 2770 3659 5288 8
Size: 89 18 355 889 1629 43
Fragments arranged by size:
1629 1425 889 355 275 192 183 103
89 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 2205 2308 2770 1537
Size: 275 210 183 103 462 4090
Fragments arranged by size:
4090 462 275 210 183 103
EarI CTCTTCn'nnn_
Cuts at: 1353 3253 1353
Size: 1900 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2356 2356
Size: 5323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2153 2276 2372 3386 3983 4770
Size: 393 509 12 123 96 1014 597 787
Cuts at: 4770 5038 1239
Size: 268 1524
Fragments arranged by size:
1524 1014 787 597 509 393 268 123
96 12
EcoO109I rG'GnC_Cy
Cuts at: 2012 2012
Size: 5323
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 2036 2069 2091 2140 2162 2223 2300 4470
Size: 358 33 22 49 22 61 77 2170
Cuts at: 4470 1678
Size: 2531
Fragments arranged by size:
2531 2170 358 77 61 49 33 22
22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373
Size: 32 135 32 2392 32 42 32 2626
Fragments arranged by size:
2626 2392 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KasI G'GCGC_C
Cuts at: 2065 2087 2219 2065
Size: 22 132 5169
Fragments arranged by size:
5169 132 22
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2417 10
Size: 54 1425 343 567 18 2916
Fragments arranged by size:
2916 1425 567 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 3281 3640 3799 518
Size: 648 2115 359 159 2042
Fragments arranged by size:
2115 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2142 2739 3416 4564 4809 1606
Size: 421 115 597 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 597 421 245 115
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 5323
NarI GG'CG_CC
Cuts at: 2066 2088 2220 2066
Size: 22 132 5169
Fragments arranged by size:
5169 132 22
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4771
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2536 2681 4226 1132
Size: 97 1307 145 1545 2229
Fragments arranged by size:
2229 1545 1307 145 97
PasI CC'CwG_GG
Cuts at: 1348 2070 1348
Size: 722 4601
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2488 3392 3424 4905 4937 2456
Size: 32 904 32 1481 32 2842
Fragments arranged by size:
2842 1481 904 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PspOMI G'GGCC_C
Cuts at: 2012 2012
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2675 830
Size: 1845 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
SacI G_AGCT'C
Cuts at: 2358 2358
Size: 5323
SacII CC_GC'GG
Cuts at: 2028 2028
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2671 3806 4487 4678 826
Size: 219 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2067 2089 2221 2067
Size: 22 132 5169
Fragments arranged by size:
5169 132 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 2222 1700
Size: 522 4801
SmaI CCC'GGG
Cuts at: 1872 2017 2650 1872
Size: 145 633 4545
Fragments arranged by size:
4545 633 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3427 4328 4590 820
Size: 197 926 1484 901 262 1553
Fragments arranged by size:
1553 1484 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2196 2762 513
Size: 552 1131 566 3074
Fragments arranged by size:
3074 1131 566 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2113 2122 2618 3350 3367 3520 3705
Size: 1255 533 9 496 732 17 153 185
Cuts at: 3705 4150 325
Size: 445 1498
Fragments arranged by size:
1498 1255 732 533 496 445 185 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2116
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2015 2648 1870
Size: 145 633 4545
Fragments arranged by size:
4545 633 145
XmnI GAAnn'nnTTC
Cuts at: 3452 3452
Size: 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SrfI SwaI