Restriction map for
pFN21AE2290
(Circular) (Six-base) MAPSORT of: pFN21AE2290.seq Check: 5556 from: 1 to: 5779
pFN21AE2290 5779 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2344 2635 3842 5226 5494 1632
Size: 712 291 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 712 291 268
AfeI AGC'GCT
Cuts at: 2405 2405
Size: 5779
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 2029 4508 2029
Size: 2479 3300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 2299 3522 5094 2299
Size: 1223 1572 2984
Fragments arranged by size:
2984 1572 1223
ApaI G_GGCC'C
Cuts at: 2746 2746
Size: 5779
ApaLI G'TGCA_C
Cuts at: 2329 3836 4992 2329
Size: 1507 1156 3116
Fragments arranged by size:
3116 1507 1156
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AscI GG'CGCG_CC
Cuts at: 2144 2144
Size: 5779
AseI AT'TA_AT
Cuts at: 160 1032 2922 4333 160
Size: 872 1890 1411 1606
Fragments arranged by size:
1890 1606 1411 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2315 2552 2738 3104 1870
Size: 73 300 72 237 186 366 4545
Fragments arranged by size:
4545 366 300 237 186 73 72
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2311 2421 3100 4555 618
Size: 325 217 627 524 110 679 1455 1842
Fragments arranged by size:
1842 1455 679 627 524 325 217 110
BanII G_rGCy'C
Cuts at: 1391 2192 2746 3098 1391
Size: 801 554 352 4072
Fragments arranged by size:
4072 801 554 352
BbeI G_GCGC'C
Cuts at: 2315 2315
Size: 5779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2320 2354 2362 2396 3970 4004 1903
Size: 34 383 34 8 34 1574 34 3678
Fragments arranged by size:
3678 1574 383 34 34 34 34 8
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2733 4390 136
Size: 107 122 71 631 605 1061 1657 1525
Fragments arranged by size:
1657 1525 1061 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2333 2424 2746 3840 4996 1790
Size: 543 91 322 1094 1156 2573
Fragments arranged by size:
2573 1156 1094 543 322 91
BmgBI CAC'GTC
Cuts at: 1318 2164 1318
Size: 846 4933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 2258 3510
Size: 416 53 83 399 24 606 235 1252
Cuts at: 3510 4468 442
Size: 958 1753
Fragments arranged by size:
1753 1252 958 606 416 399 235 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 4439 1239
Size: 1181 2019 2579
Fragments arranged by size:
2579 2019 1181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2209 3570 493
Size: 711 1005 1361 2702
Fragments arranged by size:
2702 1361 1005 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2312 3586 3968 4603
Size: 53 83 186 936 779 1274 382 635
Cuts at: 4603 275
Size: 1451
Fragments arranged by size:
1451 1274 936 779 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2792 1186
Size: 1606 4173
BseYI C'CCAG_C
Cuts at: 2077 2113 4982 5478 2077
Size: 36 2869 496 2378
Fragments arranged by size:
2869 2378 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2191 1163
Size: 573 455 4751
Fragments arranged by size:
4751 573 455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 2182 2418 3229 3990 5018
Size: 275 186 105 76 236 811 761 1028
Cuts at: 5018 1540
Size: 2301
Fragments arranged by size:
2301 1028 811 761 275 236 186 105
76
BsiHKAI G_wGCw'C
Cuts at: 2192 2333 2640 3840 3925 4996 2192
Size: 141 307 1200 85 1071 2975
Fragments arranged by size:
2975 1200 1071 307 141 85
BsiWI C'GTAC_G
Cuts at: 2280 2280
Size: 5779
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2039 2452 2774 2039
Size: 413 322 5044
Fragments arranged by size:
5044 413 322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2333 2424 2640 2746 3098 3840
Size: 399 402 141 91 216 106 352 742
Cuts at: 3840 3925 4996 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 352 216 141
106 91 85
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 2808 3668 5398 2808
Size: 860 1730 3189
Fragments arranged by size:
3189 1730 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2247 2258 2601 3134 844
Size: 1403 11 343 533 3489
Fragments arranged by size:
3489 1403 533 343 11
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2017 4274 4448 65
Size: 1183 769 2257 174 1396
Fragments arranged by size:
2257 1396 1183 769 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2177 4423 4610 1700
Size: 110 367 2246 187 2869
Fragments arranged by size:
2869 2246 367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 2144 1925
Size: 219 5560
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2215 1184
Size: 1031 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2398
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774
Size: 1137 367 385 17 1452 86 12 346
Cuts at: 5774 1972
Size: 1977
Fragments arranged by size:
1977 1452 1137 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2957 2957
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2391 2769 513
Size: 552 939 387 378 3523
Fragments arranged by size:
3523 939 552 387 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3295 4089 4109 2728
Size: 567 794 20 4398
Fragments arranged by size:
4398 794 567 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2942 4786 809
Size: 507 1626 1844 1802
Fragments arranged by size:
1844 1802 1626 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2179 2367
Size: 54 1425 50 275 18 273 76 188
Cuts at: 2367 2466 2725 3226 4115 5744 8
Size: 99 259 501 889 1629 43
Fragments arranged by size:
1629 1425 889 501 275 273 259 188
99 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 2179 3226 1537
Size: 275 291 76 1047 4090
Fragments arranged by size:
4090 1047 291 275 76
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 2507 2878 3709 1353
Size: 1037 117 371 831 3423
Fragments arranged by size:
3423 1037 831 371 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2596 2789 4366 4740 4886 387
Size: 1064 138 1007 193 1577 374 146 1280
Fragments arranged by size:
1577 1280 1064 1007 374 193 146 138
EcoICRI GAG'CTC
Cuts at: 2190 2190
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2344 2420 2635 3842 4439 5226 5494
Size: 393 712 76 215 1207 597 787 268
Cuts at: 5494 1239
Size: 1524
Fragments arranged by size:
1524 1207 787 712 597 393 268 215
76
EcoO109I rG'GnC_Cy
Cuts at: 2247 2461 2556 2742 2893 2247
Size: 214 95 186 151 5133
Fragments arranged by size:
5133 214 186 151 95
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2138 2178 2315 2407 2443 4926 1678
Size: 460 40 137 92 36 2483 2531
Fragments arranged by size:
2531 2483 460 137 92 40 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 4420 4452 4494
Size: 32 135 32 443 32 2373 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 2373 443 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2311 2311
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2981 4893 5077 1305
Size: 1676 1912 184 2007
Fragments arranged by size:
2007 1912 1676 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 10
Size: 54 1425 343 537 3420
Fragments arranged by size:
3420 1425 537 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 3737 4096 4255 518
Size: 648 1509 1062 359 159 2042
Fragments arranged by size:
2042 1509 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2518 2599 2703 3195 3872 5020 5265
Size: 545 367 81 104 492 677 1148 245
Cuts at: 5265 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 545 492 367 245 104
81
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 5779
NarI GG'CG_CC
Cuts at: 2312 2312
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 2004 2391 2769 513
Size: 552 939 387 378 3523
Fragments arranged by size:
3523 939 552 387 378
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2680 3137 4682 1132
Size: 97 1451 457 1545 2229
Fragments arranged by size:
2229 1545 1451 457 97
PasI CC'CwG_GG
Cuts at: 1348 2716 1348
Size: 1368 4411
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2299 1108
Size: 1104 87 4588
Fragments arranged by size:
4588 1104 87
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PmlI CAC'GTG
Cuts at: 2209 2209
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 2245 3848 3880 5361 5393 2213
Size: 32 1603 32 1481 32 2599
Fragments arranged by size:
2599 1603 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2556 2893 2247
Size: 309 337 5133
Fragments arranged by size:
5133 337 309
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2742 2742
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2598 3131 830
Size: 1768 533 3478
Fragments arranged by size:
3478 1768 533
PvuI CG_AT'CG
Cuts at: 2001 2418 2001
Size: 417 5362
PvuII CAG'CTG
Cuts at: 2599 2599
Size: 5779
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 5779
SacI G_AGCT'C
Cuts at: 2192 2192
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2390 2507 2390
Size: 117 5662
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 2546 4027 5739 2546
Size: 1481 1712 2586
Fragments arranged by size:
2586 1712 1481
SexAI A'CCwGG_T
Cuts at: 2558 2558
Size: 5779
SfcI C'TryA_G
Cuts at: 826 1045 2594 3127 4262 4943 5134 826
Size: 219 1549 533 1135 681 191 1471
Fragments arranged by size:
1549 1471 1135 681 533 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2733 2733
Size: 5779
SfoI GGC'GCC
Cuts at: 2313 2313
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 2554 2740 3106 1872
Size: 682 186 366 4545
Fragments arranged by size:
4545 682 366 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 2680 3137 1132
Size: 1548 457 3774
Fragments arranged by size:
3774 1548 457
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2962 1517
Size: 1445 4334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 2769 2939 3218 513
Size: 552 939 387 378 170 279 3074
Fragments arranged by size:
3074 939 552 387 378 279 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 3806 3823 3976 4161 4606 325
Size: 1255 654 1572 17 153 185 445 1498
Fragments arranged by size:
1572 1498 1255 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 4025 5737
Size: 274 80 33 51 1443 567 1481 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1712 1481 1443 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 5739 5771 2153
Size: 32 3554 32 2161
Fragments arranged by size:
3554 2161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2552 2738 3104 1870
Size: 682 186 366 4545
Fragments arranged by size:
4545 682 366 186
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI SacII SanDI SrfI
SwaI XcmI