Restriction map for
pFN21AE2289
(Circular) (Six-base) MAPSORT of: pFN21AE2289.seq Check: 9570 from: 1 to: 6229
pFN21AE2289 6229 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2703 3027 3324 3345 3572 1937
Size: 766 324 297 21 227 4594
Fragments arranged by size:
4594 766 324 297 227 21
Acc65I G'GTAC_C
Cuts at: 2112 3550 2112
Size: 1438 4791
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3220 4292 5676 5944 1632
Size: 1588 1072 1384 268 1917
Fragments arranged by size:
1917 1588 1384 1072 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 2098 4958 2098
Size: 2860 3369
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2469 1184
Size: 1285 4944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 2339
Size: 32 6197
AlwNI CAG_nnn'CTG
Cuts at: 2020 3972 5544 2020
Size: 1952 1572 2705
Fragments arranged by size:
2705 1952 1572
ApaI G_GGCC'C
Cuts at: 2239 2904 2969 2239
Size: 665 65 5499
Fragments arranged by size:
5499 665 65
ApaLI G'TGCA_C
Cuts at: 2567 4286 5442 2567
Size: 1719 1156 3354
Fragments arranged by size:
3354 1719 1156
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3087 4783 160
Size: 872 2055 1696 1606
Fragments arranged by size:
2055 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 3554 1870
Size: 73 436 1175 4545
Fragments arranged by size:
4545 1175 436 73
BamHI G'GATC_C
Cuts at: 2269 2754 3559 3926 2269
Size: 485 805 367 4572
Fragments arranged by size:
4572 805 485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2112 3351 3550 5005
Size: 325 217 627 296 29 1239 199 1455
Cuts at: 5005 618
Size: 1842
Fragments arranged by size:
1842 1455 1239 627 325 296 217 199
29
BanII G_rGCy'C
Cuts at: 1391 2239 2904 2969 3003 3197 3548 1391
Size: 848 665 65 34 194 351 4072
Fragments arranged by size:
4072 848 665 351 194 65 34
BbeI G_GCGC'C
Cuts at: 2087 3355 2087
Size: 1268 4961
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 2586 2918 928
Size: 971 392 295 332 4239
Fragments arranged by size:
4239 971 392 332 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3484 4121 5331 3484
Size: 637 1210 4382
Fragments arranged by size:
4382 1210 637
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 2693 4840
Size: 107 122 71 631 605 562 459 2147
Cuts at: 4840 136
Size: 1525
Fragments arranged by size:
2147 1525 631 605 562 459 122 107
71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2239 2571 2670 2832 2841 2904 2969 4290
Size: 449 332 99 162 9 63 65 1321
Cuts at: 4290 5446 1790
Size: 1156 2573
Fragments arranged by size:
2573 1321 1156 449 332 162 99 65
63 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2693 2828 2844
Size: 416 53 83 399 24 1276 135 16
Cuts at: 2844 3960 4918 442
Size: 1116 958 1753
Fragments arranged by size:
1753 1276 1116 958 416 399 135 83
53 24 16
BmtI G_CTAG'C
Cuts at: 1056 3224 1056
Size: 2168 4061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2268 2268
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 2431 4889 1239
Size: 1096 96 2458 2579
Fragments arranged by size:
2579 2458 1096 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 3352 4036 4418
Size: 53 83 186 936 551 1268 684 382
Cuts at: 4418 5053 275
Size: 635 1451
Fragments arranged by size:
1451 1268 936 684 635 551 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 3057 3087 1178
Size: 30 165 30 936 30 688 30 4320
Fragments arranged by size:
4320 936 688 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6229
BseYI C'CCAG_C
Cuts at: 2026 2305 2557 2896 5432 5928 2026
Size: 279 252 339 2536 496 2327
Fragments arranged by size:
2536 2327 496 339 279 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2717 1163
Size: 573 729 252 4675
Fragments arranged by size:
4675 729 573 252
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3679 4440 5468 1540
Size: 275 186 1678 761 1028 2301
Fragments arranged by size:
2301 1678 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2571 4290 4375 5446 2571
Size: 1719 85 1071 3354
Fragments arranged by size:
3354 1719 1071 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2571 2670 2832 2841 2904 2969
Size: 399 449 332 99 162 9 63 65
Cuts at: 2969 3003 3197 3548 4290 4375 5446 1391
Size: 34 194 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 351 332 194
162 99 85 65 63 34 9
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2628 3584 844
Size: 1514 270 956 3489
Fragments arranged by size:
3489 1514 956 270
BsrBI CCG'CTC
Cuts at: 1394 1823 4116 1394
Size: 429 2293 3507
Fragments arranged by size:
3507 2293 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2422 2628 4724 4898 65
Size: 1183 1174 206 2096 174 1396
Fragments arranged by size:
2096 1396 1183 1174 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2715 4873 5060 1700
Size: 110 905 2158 187 2869
Fragments arranged by size:
2869 2158 905 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 2896
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2135 2269 2754 3559 3926 4311 4328 5780
Size: 163 134 485 805 367 385 17 1452
Cuts at: 5780 5866 5878 6224 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 805 485 385 367 346 163
134 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3469 3469
Size: 6229
BtgI C'CryG_G
Cuts at: 513 1065 2088 2479 2608 2905 513
Size: 552 1023 391 129 297 3837
Fragments arranged by size:
3837 1023 552 391 297 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 2569 3215 3745 4539 4559 2569
Size: 646 530 794 20 4239
Fragments arranged by size:
4239 794 646 530 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 3316 3676
Size: 54 1425 50 275 18 652 834 360
Cuts at: 3676 4565 6194 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 834 652 360 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 1537
Size: 275 1864 4090
Fragments arranged by size:
4090 1864 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2771 2802 4159 1353
Size: 1418 31 1357 3423
Fragments arranged by size:
3423 1418 1357 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 4816 5190 5336 387
Size: 1064 138 867 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 867 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2335 2431 3220 4292 4889 5676 5944
Size: 393 703 96 789 1072 597 787 268
Cuts at: 5944 1239
Size: 1524
Fragments arranged by size:
1524 1072 789 787 703 597 393 268
96
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 2993 3188 2130
Size: 863 195 5171
Fragments arranged by size:
5171 863 195
EcoO109I rG'GnC_Cy
Cuts at: 2062 2235 2601 2686 2900 2901 2966 2062
Size: 173 366 85 214 1 65 5325
Fragments arranged by size:
5325 366 214 173 85 65 1
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
EcoRV GAT'ATC
Cuts at: 2161 2161
Size: 6229
FspI TGC'GCA
Cuts at: 2624 4735 2624
Size: 2111 4118
HaeII r_GCGC'y
Cuts at: 1678 2087 3082 3355 5376 1678
Size: 409 995 273 2021 2531
Fragments arranged by size:
2531 2021 995 409 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2746 2778 3403
Size: 32 135 32 512 32 630 32 625
Cuts at: 3403 3435 4870 4902 4944 4976 1373
Size: 32 1435 32 42 32 2626
Fragments arranged by size:
2626 1435 630 625 512 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2083 3351 2083
Size: 1268 4961
KpnI G_GTAC'C
Cuts at: 2116 3554 2116
Size: 1438 4791
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5343 5527 1305
Size: 4038 184 2007
Fragments arranged by size:
4038 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2739 4187 4546 4705 518
Size: 648 1573 1448 359 159 2042
Fragments arranged by size:
2042 1573 1448 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2218 2313 3645 4322 5470 5715 1606
Size: 414 198 95 1332 677 1148 245 2120
Fragments arranged by size:
2120 1332 1148 677 414 245 198 95
NarI GG'CG_CC
Cuts at: 2084 3352 2084
Size: 1268 4961
NcoI C'CATG_G
Cuts at: 513 1065 2479 513
Size: 552 1414 4263
Fragments arranged by size:
4263 1414 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 3220 1052
Size: 2168 4061
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 2656 3452 1108
Size: 1536 12 796 3885
Fragments arranged by size:
3885 1536 796 12
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2371 4298 4330 5811 5843 2339
Size: 32 1927 32 1481 32 2725
Fragments arranged by size:
2725 1927 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2601 2062
Size: 539 5690
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2235 2900 2965 2235
Size: 665 65 5499
Fragments arranged by size:
5499 665 65
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2020 2218 2020
Size: 198 6031
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2117 3432 3577 4712 5393 5584 826
Size: 219 1072 1315 145 1135 681 191 1471
Fragments arranged by size:
1471 1315 1135 1072 681 219 191 145
SfiI GGCCn_nnn'nGGCC
Cuts at: 2693 2693
Size: 6229
SfoI GGC'GCC
Cuts at: 2085 3353 2085
Size: 1268 4961
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2381 3556 1872
Size: 509 1175 4545
Fragments arranged by size:
4545 1175 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SrfI GCCC'GGGC
Cuts at: 2381 2381
Size: 6229
SspI AAT'ATT
Cuts at: 5 52 3397 4153 5
Size: 47 3345 756 2081
Fragments arranged by size:
3345 2081 756 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2479 3486 3668 513
Size: 552 1414 1007 182 3074
Fragments arranged by size:
3074 1414 1007 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2751 4256 4273 4426 4611 5056 325
Size: 1255 1171 1505 17 153 185 445 1498
Fragments arranged by size:
1505 1498 1255 1171 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4475 6187 96
Size: 274 80 33 51 1443 2498 1712 138
Fragments arranged by size:
2498 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 2924 2956 3057 3089 6189 6221 2593
Size: 32 299 32 101 32 3100 32 2601
Fragments arranged by size:
3100 2601 299 101 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 2128 3565 2128
Size: 1437 4792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2765 2765
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2379 3554 1870
Size: 509 1175 4545
Fragments arranged by size:
4545 1175 509
XmnI GAAnn'nnTTC
Cuts at: 3397 4358 3397
Size: 961 5268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SwaI