Restriction map for
pFN21AE2286
(Circular) (Six-base) MAPSORT of: pFN21AE2286.seq Check: 3661 from: 1 to: 6037
pFN21AE2286 6037 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3027 3027
Size: 6037
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3380 1937
Size: 1443 4594
Acc65I G'GTAC_C
Cuts at: 3358 3358
Size: 6037
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4100 5484 5752 1632
Size: 2468 1384 268 1917
Fragments arranged by size:
2468 1917 1384 268
AfeI AGC'GCT
Cuts at: 2113 2113
Size: 6037
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 2134 2285 2757 4936 5913 1203
Size: 931 151 472 2179 977 1327
Fragments arranged by size:
2179 1327 977 931 472 151
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 2073 1166
Size: 907 5130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2784 2816 2784
Size: 32 6005
AlwNI CAG_nnn'CTG
Cuts at: 3780 5352 3780
Size: 1572 4465
ApaI G_GGCC'C
Cuts at: 2699 2855 2699
Size: 156 5881
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 3346 3572 3724 5833 1493
Size: 1853 226 152 2109 1697
Fragments arranged by size:
2109 1853 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2923 3362 1870
Size: 73 980 439 4545
Fragments arranged by size:
4545 980 439 73
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2326 3358 4813 618
Size: 325 217 627 332 207 1032 1455 1842
Fragments arranged by size:
1842 1455 1032 627 332 325 217 207
BanII G_rGCy'C
Cuts at: 1391 2255 2699 2750 2788 2855 3356 1391
Size: 864 444 51 38 67 501 4072
Fragments arranged by size:
4072 864 501 444 67 51 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 928
Size: 971 300 4766
Fragments arranged by size:
4766 971 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 2346 1548
Size: 798 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4648 136
Size: 107 122 71 631 605 2976 1525
Fragments arranged by size:
2976 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 3306 3454 3306
Size: 148 5889
Bme1580I G_kGCm'C
Cuts at: 1790 2699 2855 4098 5254 1790
Size: 909 156 1243 1156 2573
Fragments arranged by size:
2573 1243 1156 909 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 2625 3329
Size: 416 53 83 399 24 908 300 704
Cuts at: 3329 3768 4726 442
Size: 439 958 1753
Fragments arranged by size:
1753 958 908 704 439 416 399 300
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4697 1239
Size: 3458 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2533 4700 882
Size: 617 1034 2167 2219
Fragments arranged by size:
2219 2167 1034 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 3828 493
Size: 711 933 1691 2702
Fragments arranged by size:
2702 1691 933 711
BsaBI GATnn'nnATC
Cuts at: 6031 6031
Size: 6037
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3844 4226 4861 275
Size: 53 83 186 936 2311 382 635 1451
Fragments arranged by size:
2311 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2121 2151 2291 2321 2364
Size: 30 165 30 718 30 140 30 43
Cuts at: 2364 2394 2406 2436 2706 2736 2784 2814 1178
Size: 30 12 30 270 30 48 30 4401
Fragments arranged by size:
4401 718 270 165 140 48 43 30
30 30 30 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3056 3059 3200 1186
Size: 1870 3 141 4023
Fragments arranged by size:
4023 1870 141 3
BseYI C'CCAG_C
Cuts at: 2108 2330 2688 5240 5736 2108
Size: 222 358 2552 496 2409
Fragments arranged by size:
2552 2409 496 358 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 3005 1163
Size: 573 717 552 4195
Fragments arranged by size:
4195 717 573 552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4248 5276 1540
Size: 275 186 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 2750 3098 4098 4183 5254 2460
Size: 290 348 1000 85 1071 3243
Fragments arranged by size:
3243 1071 1000 348 290 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2255 2460 2699 2750 2788 2855 3098
Size: 399 465 205 239 51 38 67 243
Cuts at: 3098 3356 4098 4183 5254 1391
Size: 258 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 399 258 243 239
205 85 67 51 38
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 3926 5656 3926
Size: 1730 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3027 3392 844
Size: 2183 365 3489
Fragments arranged by size:
3489 2183 365
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2438 4532 4706 65
Size: 1183 1190 2094 174 1396
Fragments arranged by size:
2094 1396 1190 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4681 4868 1700
Size: 110 250 2621 187 2869
Fragments arranged by size:
2869 2621 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 3285 96
Size: 3189 2848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6037
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 3005 1184
Size: 1821 4216
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2481 2514 3314 3367 3734 4119 4136 5588
Size: 509 33 800 53 367 385 17 1452
Cuts at: 5588 5674 5686 6032 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 800 509 385 367 346 86
53 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 3183 3183
Size: 6037
BtgI C'CryG_G
Cuts at: 513 1065 2218 2340 2863 513
Size: 552 1153 122 523 3687
Fragments arranged by size:
3687 1153 552 523 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5298
Fragments arranged by size:
5298 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 2933 3211 3553 4347 4367 2814
Size: 119 278 342 794 20 4484
Fragments arranged by size:
4484 794 342 278 119 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DraIII CAC_nnn'GTG
Cuts at: 2319 2319
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2651 5044 809
Size: 507 1335 2393 1802
Fragments arranged by size:
2393 1802 1335 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3484 4373 6002
Size: 54 1425 50 275 18 1654 889 1629
Cuts at: 6002 8
Size: 43
Fragments arranged by size:
1654 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 3062 3077 3967 1353
Size: 795 914 15 890 3423
Fragments arranged by size:
3423 914 890 795 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2748 2748
Size: 6037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4100 4697 5484 5752 1239
Size: 393 2468 597 787 268 1524
Fragments arranged by size:
2468 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2127 2127
Size: 6037
EcoO109I rG'GnC_Cy
Cuts at: 2396 2491 2577 2696 2852 2871 3036 2396
Size: 95 86 119 156 19 165 5397
Fragments arranged by size:
5397 165 156 119 95 86 19
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
EcoRV GAT'ATC
Cuts at: 2446 2446
Size: 6037
FspI TGC'GCA
Cuts at: 2813 4543 2813
Size: 1730 4307
HaeII r_GCGC'y
Cuts at: 1678 2115 5184 1678
Size: 437 3069 2531
Fragments arranged by size:
3069 2531 437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2406 2438 2513
Size: 32 135 32 475 32 327 32 75
Cuts at: 2513 2545 4678 4710 4752 4784 1373
Size: 32 2133 32 42 32 2626
Fragments arranged by size:
2626 2133 475 327 135 75 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 3381 3627 669
Size: 988 281 883 560 246 3079
Fragments arranged by size:
3079 988 883 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 3362 3362
Size: 6037
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2488 3123 3186 5151 5335 1305
Size: 1183 635 63 1965 184 2007
Fragments arranged by size:
2007 1965 1183 635 184 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4215
Fragments arranged by size:
4215 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 3995 4354 4513 518
Size: 648 907 1922 359 159 2042
Fragments arranged by size:
2042 1922 907 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3453 4130 5278 5523 1606
Size: 1847 677 1148 245 2120
Fragments arranged by size:
2120 1847 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2218 2340 513
Size: 552 1153 122 4210
Fragments arranged by size:
4210 1153 552 122
NdeI CA'TA_TG
Cuts at: 387 2665 387
Size: 2278 3759
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2273 2289 2761 3395 4940 1132
Size: 97 1044 16 472 634 1545 2229
Fragments arranged by size:
2229 1545 1044 634 472 97 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 2285 2757 4936 2285
Size: 472 2179 3386
Fragments arranged by size:
3386 2179 472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2319 1108
Size: 1211 4826
PfoI T'CCnGG_A
Cuts at: 2232 2741 2232
Size: 509 5528
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PmlI CAC'GTG
Cuts at: 2137 2137
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2784 2816 4106 4138 5619 5651 2784
Size: 32 1290 32 1481 32 3170
Fragments arranged by size:
3170 1481 1290 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2396 2491 2577 2871 3036 2396
Size: 95 86 294 165 5397
Fragments arranged by size:
5397 294 165 95 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PspOMI G'GGCC_C
Cuts at: 2695 2851 2695
Size: 156 5881
PstI C_TGCA'G
Cuts at: 830 2809 3139 3389 830
Size: 1979 330 250 3478
Fragments arranged by size:
3478 1979 330 250
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
SacI G_AGCT'C
Cuts at: 2750 2750
Size: 6037
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SbfI CC_TGCA'GG
Cuts at: 3139 3389 3139
Size: 250 5787
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 2581 2734 2805 2875 3135 3270 3385
Size: 219 1536 153 71 70 260 135 115
Cuts at: 3385 4520 5201 5392 826
Size: 1135 681 191 1471
Fragments arranged by size:
1536 1471 1135 681 260 219 191 153
135 115 71 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 3364 1872
Size: 1492 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 2229 3961 5
Size: 47 2177 1732 2081
Fragments arranged by size:
2177 2081 1732 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6037
StyI C'CwwG_G
Cuts at: 513 1065 2218 2340 2532 2700 3476 513
Size: 552 1153 122 192 168 776 3074
Fragments arranged by size:
3074 1153 776 552 192 168 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2307 2316 2344 2406 2540 2876
Size: 1255 483 244 9 28 62 134 336
Cuts at: 2876 2995 4064 4081 4234 4419 4864 325
Size: 119 1069 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1069 483 445 336 244 185
153 134 119 62 28 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 4283 5995
Size: 274 80 33 51 1443 1308 998 1712
Cuts at: 5995 96
Size: 138
Fragments arranged by size:
1712 1443 1308 998 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2291 2323 2706 2738 3280 3312 5997
Size: 32 138 32 383 32 542 32 2685
Cuts at: 5997 6029 2121
Size: 32 2129
Fragments arranged by size:
2685 2129 542 383 138 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2728 1316
Size: 1412 4625
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2338 2347 2638 2011
Size: 327 9 291 5410
Fragments arranged by size:
5410 327 291 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 3362 1870
Size: 1492 4545
XmnI GAAnn'nnTTC
Cuts at: 2374 2959 4166 2374
Size: 585 1207 4245
Fragments arranged by size:
4245 1207 585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI