Restriction map for
pFN21AE2284
(Circular) (Six-base) MAPSORT of: pFN21AE2284.seq Check: 951 from: 1 to: 7036
pFN21AE2284 7036 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4379 1937
Size: 2442 4594
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 5163 5536 1563
Size: 3600 373 3063
Fragments arranged by size:
3600 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 3036 3332 4071 5099 6483 6751 1632
Size: 926 478 296 739 1028 1384 268 1917
Fragments arranged by size:
1917 1384 1028 926 739 478 296 268
AfeI AGC'GCT
Cuts at: 2617 2617
Size: 7036
AflII C'TTAA_G
Cuts at: 820 1017 4055 820
Size: 197 3038 3801
Fragments arranged by size:
3801 3038 197
AflIII A'CryG_T
Cuts at: 1203 5935 6912 1203
Size: 4732 977 1327
Fragments arranged by size:
4732 1327 977
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3043 1184
Size: 1859 5177
AlwNI CAG_nnn'CTG
Cuts at: 2339 2806 3156 4779 6351 2339
Size: 467 350 1623 1572 3024
Fragments arranged by size:
3024 1623 1572 467 350
ApaI G_GGCC'C
Cuts at: 2105 2992 2105
Size: 887 6149
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 4345 4571 4723 6832 1493
Size: 2852 226 152 2109 1697
Fragments arranged by size:
2852 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5590 160
Size: 872 4558 1606
Fragments arranged by size:
4558 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2643 3259 3273 3837 4361 1870
Size: 73 105 595 616 14 564 524 4545
Fragments arranged by size:
4545 616 595 564 524 105 73 14
AvrII C'CTAG_G
Cuts at: 3827 3827
Size: 7036
BamHI G'GATC_C
Cuts at: 3009 4366 4733 3009
Size: 1357 367 5312
Fragments arranged by size:
5312 1357 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 3302 3679 4357 5812
Size: 325 217 627 408 1107 377 678 1455
Cuts at: 5812 618
Size: 1842
Fragments arranged by size:
1842 1455 1107 678 627 408 377 325
217
BanII G_rGCy'C
Cuts at: 1391 2045 2105 2992 3489 3620 4286 4355 1391
Size: 654 60 887 497 131 666 69 4072
Fragments arranged by size:
4072 887 666 654 497 131 69 60
BbeI G_GCGC'C
Cuts at: 3683 3683
Size: 7036
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6065
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2684 2718 5227 5261 1903
Size: 34 50 34 663 34 2509 34 3678
Fragments arranged by size:
3678 2509 663 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2314 3356 3532 4928 6138 2314
Size: 1042 176 1396 1210 3212
Fragments arranged by size:
3212 1396 1210 1042 176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2023 2109 2366
Size: 107 122 71 631 605 351 86 257
Cuts at: 2366 2811 5647 136
Size: 445 2836 1525
Fragments arranged by size:
2836 1525 631 605 445 351 257 122
107 86 71
BglII A'GATC_T
Cuts at: 7031 7031
Size: 7036
BlpI GC'TnA_GC
Cuts at: 3853 4453 3853
Size: 600 6436
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2992 4330 5097 6253 1790
Size: 315 887 1338 767 1156 2573
Fragments arranged by size:
2573 1338 1156 887 767 315
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7036
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 3675 4767
Size: 416 53 83 399 24 2237 21 1092
Cuts at: 4767 5725 442
Size: 958 1753
Fragments arranged by size:
2237 1753 1092 958 416 399 83 53
24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7036
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 7036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2958 3020 3257 3407 5696 1239
Size: 1076 643 62 237 150 2289 2579
Fragments arranged by size:
2579 2289 1076 643 237 150 62
Bpu10I CC'TnA_GC
Cuts at: 2673 2673
Size: 7036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 5161 6026 6324 2579
Size: 2582 865 298 3291
Fragments arranged by size:
3291 2582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2823 5699 882
Size: 617 1324 2876 2219
Fragments arranged by size:
2876 2219 1324 617
BsaAI yAC'GTr
Cuts at: 493 1204 4827 493
Size: 711 3623 2702
Fragments arranged by size:
3623 2702 711
BsaBI GATnn'nnATC
Cuts at: 7030 7030
Size: 7036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3680 4230 4843 5225
Size: 53 83 186 936 2147 550 613 382
Cuts at: 5225 5860 275
Size: 635 1451
Fragments arranged by size:
2147 1451 936 635 613 550 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2637 5469 5867 6141 6288 6699 2637
Size: 2832 398 274 147 411 2974
Fragments arranged by size:
2974 2832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2955 2985 3471 3501 3679
Size: 30 165 30 1552 30 486 30 178
Cuts at: 3679 3709 1178
Size: 30 4505
Fragments arranged by size:
4505 1552 486 178 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2159 2198 2201 3038 3188 3222 3687
Size: 970 3 39 3 837 150 34 465
Cuts at: 3687 3811 4251 4290 1186
Size: 124 440 39 3932
Fragments arranged by size:
3932 970 837 465 440 150 124 39
39 34 3 3
BseYI C'CCAG_C
Cuts at: 3717 3797 4138 6239 6735 3717
Size: 80 341 2101 496 4018
Fragments arranged by size:
4018 2101 496 341 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3230 3764 3906 1163
Size: 573 1494 534 142 4293
Fragments arranged by size:
4293 1494 573 534 142
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4486 5247 6275 1540
Size: 275 186 2485 761 1028 2301
Fragments arranged by size:
2485 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5097 5182 6253 5097
Size: 85 1071 5880
Fragments arranged by size:
5880 1071 85
BsmI GAATG_Cn'
Cuts at: 4547 4640 4547
Size: 93 6943
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2105 2992 3489 3620 4286 4330
Size: 399 255 60 887 497 131 666 44
Cuts at: 4330 4355 5097 5182 6253 1391
Size: 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 887 742 666 497 399 255
131 85 60 44 25
BspEI T'CCGG_A
Cuts at: 6699 6699
Size: 7036
BspHI T'CATG_A
Cuts at: 4925 6655 4925
Size: 1730 5306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4391 844
Size: 3547 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 4923 1394
Size: 429 2029 1071 3507
Fragments arranged by size:
3507 2029 1071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5531 5705 65
Size: 1183 4283 174 1396
Fragments arranged by size:
4283 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2553 2832 3030 3654 5680 5867 1700
Size: 110 743 279 198 624 2026 187 2869
Fragments arranged by size:
2869 2026 743 624 279 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7036
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7036
BssSI C'ACGA_G
Cuts at: 2038 3206 5096 6108 2038
Size: 1168 1890 1012 2966
Fragments arranged by size:
2966 1890 1168 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7036
BstBI TT'CG_AA
Cuts at: 5840 5840
Size: 7036
BstEII G'GTnAC_C
Cuts at: 1184 2956 3842 1184
Size: 1772 886 4378
Fragments arranged by size:
4378 1772 886
BstYI r'GATC_y
Cuts at: 1972 3009 3926 4366 4733 5118 5135 6587 6673
Size: 1037 917 440 367 385 17 1452 86
Cuts at: 6673 6685 7031 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1037 917 440 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2517 4252 2517
Size: 1735 5301
BtgI C'CryG_G
Cuts at: 513 1065 2012 2712 3063 3566 4164 513
Size: 552 947 700 351 503 598 3385
Fragments arranged by size:
3385 947 700 598 552 503 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6297
Fragments arranged by size:
6297 683 56
BtsI GCAGTG_nn'
Cuts at: 2910 3605 3997 4086 4552 5346 5366 2910
Size: 695 392 89 466 794 20 4580
Fragments arranged by size:
4580 794 695 466 392 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7036
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 4340 4340
Size: 7036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6043 809
Size: 507 4727 1802
Fragments arranged by size:
4727 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2745 2834 2966
Size: 54 1425 50 275 18 915 89 132
Cuts at: 2966 3491 3578 3731 4483 5372 7001 8
Size: 525 87 153 752 889 1629 43
Fragments arranged by size:
1629 1425 915 889 752 525 275 153
132 89 87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4483 1537
Size: 275 2671 4090
Fragments arranged by size:
4090 2671 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2133 2151 3055 3885 4966 1353
Size: 732 48 18 904 830 1081 3423
Fragments arranged by size:
3423 1081 904 830 732 48 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5623 5997 6143 387
Size: 1064 138 4034 374 146 1280
Fragments arranged by size:
4034 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2558 2958 3020 3036 3257 3332
Size: 393 683 243 400 62 16 221 75
Cuts at: 3332 3407 4071 5099 5696 6483 6751 1239
Size: 75 664 1028 597 787 268 1524
Fragments arranged by size:
1524 1028 787 683 664 597 400 393
268 243 221 75 75 62 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3826 3837 4291 3826
Size: 11 454 6571
Fragments arranged by size:
6571 454 11
EcoO109I rG'GnC_Cy
Cuts at: 2083 2101 2161 2299 2659 2945 2988 3256 3316
Size: 18 60 138 360 286 43 268 60
Cuts at: 3316 3628 3939 4209 2083
Size: 312 311 270 4910
Fragments arranged by size:
4910 360 312 311 286 270 268 138
60 60 43 18
EcoRI G'AATT_C
Cuts at: 4345 4723 4345
Size: 378 6658
EcoRV GAT'ATC
Cuts at: 3466 3466
Size: 7036
FspI TGC'GCA
Cuts at: 5542 5542
Size: 7036
HaeII r_GCGC'y
Cuts at: 1678 2619 3683 6183 1678
Size: 941 1064 2500 2531
Fragments arranged by size:
2531 2500 1064 941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2513 2545 2782 2814 2859
Size: 32 135 32 941 32 237 32 45
Cuts at: 2859 2891 3323 3355 3490 3522 5677 5709 5751
Size: 32 432 32 135 32 2155 32 42
Cuts at: 5751 5783 1373
Size: 32 2626
Fragments arranged by size:
2626 2155 941 432 237 135 135 45
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2496 4380 4626 669
Size: 988 281 558 1884 246 3079
Fragments arranged by size:
3079 1884 988 558 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7036
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KasI G'GCGC_C
Cuts at: 3679 3679
Size: 7036
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 4635 4635
Size: 7036
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3333 3475 6150 6334 1305
Size: 2028 142 2675 184 2007
Fragments arranged by size:
2675 2028 2007 184 142
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3733 10
Size: 54 1425 343 1901 3313
Fragments arranged by size:
3313 1901 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4010 4994 5353 5512 518
Size: 648 2844 984 359 159 2042
Fragments arranged by size:
2844 2042 984 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2755 3791 3874 4108 4201 4452 5129
Size: 408 741 1036 83 234 93 251 677
Cuts at: 5129 6277 6522 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1036 741 677 408 251 245
234 93 83
NaeI GCC'GGC
Cuts at: 3032 3032
Size: 7036
NarI GG'CG_CC
Cuts at: 3680 3680
Size: 7036
NcoI C'CATG_G
Cuts at: 513 1065 4164 513
Size: 552 3099 3385
Fragments arranged by size:
3385 3099 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7036
NgoMIV G'CCGG_C
Cuts at: 3030 3030
Size: 7036
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7036
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 4394 5939 1132
Size: 97 3165 1545 2229
Fragments arranged by size:
3165 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7036
PciI A'CATG_T
Cuts at: 5935 5935
Size: 7036
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3299 1108
Size: 2191 4845
PfoI T'CCnGG_A
Cuts at: 2997 2997
Size: 7036
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2766 2798 5105 5137 6618 6650 2766
Size: 32 2307 32 1481 32 3152
Fragments arranged by size:
3152 2307 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2945 3316 3628 4209 2083
Size: 862 371 312 581 4910
Fragments arranged by size:
4910 862 581 371 312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7036
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PspOMI G'GGCC_C
Cuts at: 2101 2988 2101
Size: 887 6149
PstI C_TGCA'G
Cuts at: 830 3081 3228 4081 4388 830
Size: 2251 147 853 307 3478
Fragments arranged by size:
3478 2251 853 307 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7036
PvuII CAG'CTG
Cuts at: 2755 3791 4108 2755
Size: 1036 317 5683
Fragments arranged by size:
5683 1036 317
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 7036
SalI G'TCGA_C
Cuts at: 1936 4378 1936
Size: 2442 4594
SanDI GG'GwC_CC
Cuts at: 2083 3628 2083
Size: 1545 5491
SbfI CC_TGCA'GG
Cuts at: 4388 4388
Size: 7036
ScaI AGT'ACT
Cuts at: 3664 5284 6996 3664
Size: 1620 1712 3704
Fragments arranged by size:
3704 1712 1620
SfcI C'TryA_G
Cuts at: 826 1045 3077 3224 3479 4077 4384 5519 6200
Size: 219 2032 147 255 598 307 1135 681
Cuts at: 6200 6391 826
Size: 191 1471
Fragments arranged by size:
2032 1471 1135 681 598 307 255 219
191 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 2109 2109
Size: 7036
SfoI GGC'GCC
Cuts at: 3681 3681
Size: 7036
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7036
SmaI CCC'GGG
Cuts at: 1872 2050 2645 3275 4363 1872
Size: 178 595 630 1088 4545
Fragments arranged by size:
4545 1088 630 595 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3837 4055 5140 6041 6303
Size: 197 926 615 1279 218 1085 901 262
Cuts at: 6303 820
Size: 1553
Fragments arranged by size:
1553 1279 1085 926 901 615 262 218
197
SnaBI TAC'GTA
Cuts at: 493 4827 493
Size: 4334 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 4394 1132
Size: 3262 3774
SspI AAT'ATT
Cuts at: 5 52 4960 5
Size: 47 4908 2081
Fragments arranged by size:
4908 2081 47
StuI AGG'CCT
Cuts at: 1517 2814 3832 3896 1517
Size: 1297 1018 64 4657
Fragments arranged by size:
4657 1297 1018 64
StyI C'CwwG_G
Cuts at: 513 1065 2241 2538 3298 3342 3759 3827 4164
Size: 552 1176 297 760 44 417 68 337
Cuts at: 4164 4475 513
Size: 311 3074
Fragments arranged by size:
3074 1176 760 552 417 337 311 297
68 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5063 5080 5233 5418 5863 325
Size: 1255 3483 17 153 185 445 1498
Fragments arranged by size:
3483 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2488 3662 5282
Size: 274 80 33 51 1443 511 1174 1620
Cuts at: 5282 6994 96
Size: 1712 138
Fragments arranged by size:
1712 1620 1443 1174 511 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4170 4202 6996 7028 4170
Size: 32 2794 32 4178
Fragments arranged by size:
4178 2794 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 4372 4372
Size: 7036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 3349 2719
Size: 630 6406
XhoI C'TCGA_G
Cuts at: 1943 3837 1943
Size: 1894 5142
XmaI C'CCGG_G
Cuts at: 1870 2048 2643 3273 4361 1870
Size: 178 595 630 1088 4545
Fragments arranged by size:
4545 1088 630 595 178
XmnI GAAnn'nnTTC
Cuts at: 2778 5165 2778
Size: 2387 4649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SapI
SexAI SrfI SwaI