Restriction map for
pFN21AE2282
(Circular) (Six-base) MAPSORT of: pFN21AE2282.seq Check: 970 from: 1 to: 5098
pFN21AE2282 5098 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2908 278
Size: 53 83 186 936 1372 2468
Fragments arranged by size:
2468 1372 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2441 1937
Size: 504 4594
Acc65I G'GTAC_C
Cuts at: 2419 2419
Size: 5098
AclI AA'CG_TT
Cuts at: 1563 3225 3598 1563
Size: 1662 373 3063
Fragments arranged by size:
3063 1662 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 3161 4545 4813 1632
Size: 418 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 418 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4901
AflIII A'CryG_T
Cuts at: 1203 2313 3997 4974 1203
Size: 1110 1684 977 1327
Fragments arranged by size:
1684 1327 1110 977
AgeI A'CCGG_T
Cuts at: 3929 3929
Size: 5098
AhdI GACnn_n'nnGTC
Cuts at: 3827 3827
Size: 5098
AleI CACnn'nnGTG
Cuts at: 1166 2256 1166
Size: 1090 4008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5098
AlwNI CAG_nnn'CTG
Cuts at: 2841 4413 2841
Size: 1572 3526
ApaI G_GGCC'C
Cuts at: 2057 2057
Size: 5098
ApaLI G'TGCA_C
Cuts at: 3155 4311 3155
Size: 1156 3942
ApoI r'AATT_y
Cuts at: 1493 2407 2633 2785 4894 1493
Size: 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3652 160
Size: 872 2620 1606
Fragments arranged by size:
2620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5098
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2423 1870
Size: 73 106 374 4545
Fragments arranged by size:
4545 374 106 73
BamHI G'GATC_C
Cuts at: 2428 2795 2428
Size: 367 4731
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 3874 618
Size: 325 217 627 632 1455 1842
Fragments arranged by size:
1842 1455 632 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2101 2417 1391
Size: 666 44 316 4072
Fragments arranged by size:
4072 666 316 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3289 3323 1903
Size: 34 50 34 1268 34 3678
Fragments arranged by size:
3678 1268 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2990 4200 2990
Size: 1210 3888
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5098
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 3709 136
Size: 107 122 71 631 605 389 1648 1525
Fragments arranged by size:
1648 1525 631 605 389 122 107 71
BglII A'GATC_T
Cuts at: 5093 5093
Size: 5098
BlpI GC'TnA_GC
Cuts at: 2515 2515
Size: 5098
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2179 3159 4315 1790
Size: 267 122 980 1156 2573
Fragments arranged by size:
2573 1156 980 267 122
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 3787 442
Size: 416 53 83 399 24 1412 958 1753
Fragments arranged by size:
1753 1412 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 3758 1239
Size: 759 1760 2579
Fragments arranged by size:
2579 1760 759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 2130 3223 4088 4386 2125
Size: 5 1093 865 298 2837
Fragments arranged by size:
2837 1093 865 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3761 882
Size: 617 2262 2219
Fragments arranged by size:
2262 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 2889 493
Size: 711 1110 575 2702
Fragments arranged by size:
2702 1110 711 575
BsaBI GATnn'nnATC
Cuts at: 5092 5092
Size: 5098
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2905 3287 3922 275
Size: 53 83 186 936 1372 382 635 1451
Fragments arranged by size:
1451 1372 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3531 3929 4203 4350 4761 3531
Size: 398 274 147 411 3868
Fragments arranged by size:
3868 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4873
Fragments arranged by size:
4873 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5098
BseYI C'CCAG_C
Cuts at: 4301 4797 4301
Size: 496 4602
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 3309 4337 1540
Size: 275 186 547 761 1028 2301
Fragments arranged by size:
2301 1028 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 3159 3244 4315 2040
Size: 1119 85 1071 2823
Fragments arranged by size:
2823 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 2609 2702 2609
Size: 93 5005
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2057 2101 2179 2417 3159 3244
Size: 399 250 17 44 78 238 742 85
Cuts at: 3244 4315 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 250 238 85 78
44 17
BspEI T'CCGG_A
Cuts at: 4761 4761
Size: 5098
BspHI T'CATG_A
Cuts at: 2987 4717 2987
Size: 1730 3368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 844
Size: 1609 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2985 1394
Size: 429 1162 3507
Fragments arranged by size:
3507 1162 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3593 3767 65
Size: 1183 2345 174 1396
Fragments arranged by size:
2345 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3742 3929 1700
Size: 110 1932 187 2869
Fragments arranged by size:
2869 1932 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5098
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5098
BssSI C'ACGA_G
Cuts at: 2178 2252 3158 4170 2178
Size: 74 906 1012 3106
Fragments arranged by size:
3106 1012 906 74
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5098
BstBI TT'CG_AA
Cuts at: 3902 3902
Size: 5098
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5098
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 5098
BstYI r'GATC_y
Cuts at: 1972 2428 2795 3180 3197 4649 4735 4747 5093
Size: 456 367 385 17 1452 86 12 346
Cuts at: 5093 1972
Size: 1977
Fragments arranged by size:
1977 1452 456 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2071 2220 513
Size: 552 1006 149 3391
Fragments arranged by size:
3391 1006 552 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4359
Fragments arranged by size:
4359 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 3408 3428 2614
Size: 794 20 4284
Fragments arranged by size:
4284 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5098
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5063
DraI TTT'AAA
Cuts at: 2402 2402
Size: 5098
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4105 809
Size: 507 2789 1802
Fragments arranged by size:
2789 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2268 2301
Size: 54 1425 50 275 18 387 51 33
Cuts at: 2301 2545 3434 5063 8
Size: 244 889 1629 43
Fragments arranged by size:
1629 1425 889 387 275 244 54 51
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2545 1537
Size: 275 733 4090
Fragments arranged by size:
4090 733 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3028 1353
Size: 1675 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3685 4059 4205 387
Size: 1064 138 2096 374 146 1280
Fragments arranged by size:
2096 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2050 3161 3758 4545 4813 1239
Size: 393 366 52 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 393 366 268 52
EcoO109I rG'GnC_Cy
Cuts at: 2053 2054 2053
Size: 1 5097
EcoRI G'AATT_C
Cuts at: 2407 2785 2407
Size: 378 4720
FspI TGC'GCA
Cuts at: 3604 3604
Size: 5098
HaeII r_GCGC'y
Cuts at: 1678 2357 4245 1678
Size: 679 1888 2531
Fragments arranged by size:
2531 1888 679
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3739 3771 3813 3845 1373
Size: 32 135 32 2167 32 42 32 2626
Fragments arranged by size:
2626 2167 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2442 2688 669
Size: 988 281 504 246 3079
Fragments arranged by size:
3079 988 504 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5098
HpaI GTT'AAC
Cuts at: 2688 2688
Size: 5098
KpnI G_GTAC'C
Cuts at: 2423 2423
Size: 5098
MfeI C'AATT_G
Cuts at: 2697 2697
Size: 5098
MluI A'CGCG_T
Cuts at: 4974 4974
Size: 5098
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4212 4396 1305
Size: 2907 184 2007
Fragments arranged by size:
2907 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 2303 10
Size: 54 1425 343 387 84 2805
Fragments arranged by size:
2805 1425 387 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2256 2339 3056 3415 3574 518
Size: 648 861 229 83 717 359 159 2042
Fragments arranged by size:
2042 861 717 648 359 229 159 83
MspA1I CmG'CkG
Cuts at: 1606 2173 2323 2514 3191 4339 4584 1606
Size: 567 150 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 245 191 150
NcoI C'CATG_G
Cuts at: 513 1065 2220 513
Size: 552 1155 3391
Fragments arranged by size:
3391 1155 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5098
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5098
NotI GC'GGCC_GC
Cuts at: 2545 2545
Size: 5098
NspI r_CATG'y
Cuts at: 1132 1229 2456 4001 1132
Size: 97 1227 1545 2229
Fragments arranged by size:
2229 1545 1227 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5098
PciI A'CATG_T
Cuts at: 3997 3997
Size: 5098
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5098
PfoI T'CCnGG_A
Cuts at: 2127 2127
Size: 5098
PmeI GTTT'AAAC
Cuts at: 2402 2402
Size: 5098
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 4680 4712 3167
Size: 32 1481 32 3553
Fragments arranged by size:
3553 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5098
PsiI TTA'TAA
Cuts at: 2668 2668
Size: 5098
PspOMI G'GGCC_C
Cuts at: 2053 2053
Size: 5098
PstI C_TGCA'G
Cuts at: 830 2328 2450 830
Size: 1498 122 3478
Fragments arranged by size:
3478 1498 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5098
PvuII CAG'CTG
Cuts at: 2323 2323
Size: 5098
SalI G'TCGA_C
Cuts at: 1936 2440 1936
Size: 504 4594
SbfI CC_TGCA'GG
Cuts at: 2450 2450
Size: 5098
ScaI AGT'ACT
Cuts at: 3346 5058 3346
Size: 1712 3386
SfcI C'TryA_G
Cuts at: 826 1045 2324 2446 3581 4262 4453 826
Size: 219 1279 122 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 681 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5098
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5098
SmaI CCC'GGG
Cuts at: 1872 2051 2425 1872
Size: 179 374 4545
Fragments arranged by size:
4545 374 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2104 2145 3202 4103 4365 820
Size: 197 926 161 41 1057 901 262 1553
Fragments arranged by size:
1553 1057 926 901 262 197 161 41
SnaBI TAC'GTA
Cuts at: 493 2889 493
Size: 2396 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5098
SphI G_CATG'C
Cuts at: 1132 2456 1132
Size: 1324 3774
SspI AAT'ATT
Cuts at: 5 52 3022 5
Size: 47 2970 2081
Fragments arranged by size:
2970 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5098
StyI C'CwwG_G
Cuts at: 513 1065 2220 2537 513
Size: 552 1155 317 3074
Fragments arranged by size:
3074 1155 552 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3125 3142 3295 3480 3925 325
Size: 1255 1545 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3344 5056 96
Size: 274 80 33 51 1443 1367 1712 138
Fragments arranged by size:
1712 1443 1367 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5058 5090 5058
Size: 32 5066
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5098
XbaI T'CTAG_A
Cuts at: 2434 2434
Size: 5098
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5098
XmaI C'CCGG_G
Cuts at: 1870 2049 2423 1870
Size: 179 374 4545
Fragments arranged by size:
4545 374 179
XmnI GAAnn'nnTTC
Cuts at: 3227 3227
Size: 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2906 276
Size: 53 83 186 936 1372 2468
Fragments arranged by size:
2468 1372 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI