Restriction map for
pFN21AE2281
(Circular) (Six-base) MAPSORT of: pFN21AE2281.seq Check: 5944 from: 1 to: 7234
pFN21AE2281 7234 bp
With 182 enzymes: SgfI *
March 28, 2012 12:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5044 278
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 2748 4577 1937
Size: 493 318 1829 4594
Fragments arranged by size:
4594 1829 493 318
Acc65I G'GTAC_C
Cuts at: 2825 4555 2825
Size: 1730 5504
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3692 3869 5297 6681 6949 1632
Size: 2060 177 1428 1384 268 1917
Fragments arranged by size:
2060 1917 1428 1384 268 177
AfeI AGC'GCT
Cuts at: 3893 3893
Size: 7234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7037
AflIII A'CryG_T
Cuts at: 1203 6133 7110 1203
Size: 4930 977 1327
Fragments arranged by size:
4930 1327 977
AgeI A'CCGG_T
Cuts at: 4034 6065 4034
Size: 2031 5203
AhdI GACnn_n'nnGTC
Cuts at: 3412 5963 3412
Size: 2551 4683
AleI CACnn'nnGTG
Cuts at: 1166 4287 1166
Size: 3121 4113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7234
AlwNI CAG_nnn'CTG
Cuts at: 2551 3506 3790 4977 6549 2551
Size: 955 284 1187 1572 3236
Fragments arranged by size:
3236 1572 1187 955 284
ApaLI G'TGCA_C
Cuts at: 4523 5291 6447 4523
Size: 768 1156 5310
Fragments arranged by size:
5310 1156 768
ApoI r'AATT_y
Cuts at: 1493 4543 4769 4921 7030 1493
Size: 3050 226 152 2109 1697
Fragments arranged by size:
3050 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5788 160
Size: 872 4756 1606
Fragments arranged by size:
4756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 4559 1870
Size: 73 2616 4545
Fragments arranged by size:
4545 2616 73
BamHI G'GATC_C
Cuts at: 2533 3303 4564 4931 2533
Size: 770 1261 367 4836
Fragments arranged by size:
4836 1261 770 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2825 4289 4555 6010 618
Size: 325 217 627 1038 1464 266 1455 1842
Fragments arranged by size:
1842 1464 1455 1038 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 3378 4350 4518 4553 1391
Size: 1987 972 168 35 4072
Fragments arranged by size:
4072 1987 972 168 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 928
Size: 971 546 5717
Fragments arranged by size:
5717 971 546
BbvCI CC'TCA_GC
Cuts at: 2957 3361 3909 2957
Size: 404 548 6282
Fragments arranged by size:
6282 548 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5425 5459 1903
Size: 34 3488 34 3678
Fragments arranged by size:
3678 3488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5126 6336 5126
Size: 1210 6024
BclI T'GATC_A
Cuts at: 1548 2787 3067 1548
Size: 1239 280 5715
Fragments arranged by size:
5715 1239 280
BfrBI ATG'CAT
Cuts at: 2818 2818
Size: 7234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5845 136
Size: 107 122 71 631 605 4173 1525
Fragments arranged by size:
4173 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2077 2419 7229 2077
Size: 342 4810 2082
Fragments arranged by size:
4810 2082 342
BlpI GC'TnA_GC
Cuts at: 4651 4651
Size: 7234
Bme1580I G_kGCm'C
Cuts at: 1790 4208 4527 5295 6451 1790
Size: 2418 319 768 1156 2573
Fragments arranged by size:
2573 2418 1156 768 319
BmgBI CAC'GTC
Cuts at: 1318 2899 1318
Size: 1581 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3530 4965 5923
Size: 416 53 83 399 24 2113 1435 958
Cuts at: 5923 442
Size: 1753
Fragments arranged by size:
2113 1753 1435 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2072 1056
Size: 1016 6218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2237 2299 2592 2725 3387 3417 3621
Size: 937 61 62 293 133 662 30 204
Cuts at: 3621 3668 3975 4271 4392 5894 1239
Size: 47 307 296 121 1502 2579
Fragments arranged by size:
2579 1502 937 662 307 296 293 204
133 121 62 61 47 30
Bpu10I CC'TnA_GC
Cuts at: 2921 2957 3361 3791 3909 4353 2921
Size: 36 404 430 118 444 5802
Fragments arranged by size:
5802 444 430 404 118 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2785 3539 5359 6224 6522 2785
Size: 754 1820 865 298 3497
Fragments arranged by size:
3497 1820 865 754 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2457 4382 5897 882
Size: 617 958 1925 1515 2219
Fragments arranged by size:
2219 1925 1515 958 617
BsaAI yAC'GTr
Cuts at: 493 1204 5025 493
Size: 711 3821 2702
Fragments arranged by size:
3821 2702 711
BsaBI GATnn'nnATC
Cuts at: 7228 7228
Size: 7234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5041 5423 6058 275
Size: 53 83 186 936 3508 382 635 1451
Fragments arranged by size:
3508 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2966 4034 4504 5667 6065 6339 6486 6897 2966
Size: 1068 470 1163 398 274 147 411 3303
Fragments arranged by size:
3303 1163 1068 470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2221 2235 2251 2265 2297
Size: 30 165 30 818 14 16 14 32
Cuts at: 2297 2327 3812 3842 1178
Size: 30 1485 30 4570
Fragments arranged by size:
4570 1485 818 165 32 30 30 30
30 16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2338 2879 3321 3710 3926 3978 4501 4504
Size: 1152 541 442 389 216 52 523 3
Cuts at: 4504 1186
Size: 3916
Fragments arranged by size:
3916 1152 541 523 442 389 216 52
3
BseYI C'CCAG_C
Cuts at: 3025 3198 3339 3704 3733 4042 6437 6933 3025
Size: 173 141 365 29 309 2395 496 3326
Fragments arranged by size:
3326 2395 496 365 309 173 141 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3069 1163
Size: 573 1333 5328
Fragments arranged by size:
5328 1333 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4035 4106 4684 5445 6473
Size: 275 186 1450 584 71 578 761 1028
Cuts at: 6473 1540
Size: 2301
Fragments arranged by size:
2301 1450 1028 761 584 578 275 186
71
BsiHKAI G_wGCw'C
Cuts at: 2382 3060 4518 4527 5295 5380 6451 2382
Size: 678 1458 9 768 85 1071 3165
Fragments arranged by size:
3165 1458 1071 768 678 85 9
BsmI GAATG_Cn'
Cuts at: 2113 2820 3795 4745 4838 2113
Size: 707 975 950 93 4509
Fragments arranged by size:
4509 975 950 707 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 3060 3378 4208 4350 4518 4527
Size: 399 592 678 318 830 142 168 9
Cuts at: 4527 4553 5295 5380 6451 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 830 742 678 592 399 318
168 142 85 26 9
BspEI T'CCGG_A
Cuts at: 2966 6897 2966
Size: 3931 3303
BspHI T'CATG_A
Cuts at: 3960 5123 6853 3960
Size: 1163 1730 4341
Fragments arranged by size:
4341 1730 1163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4589 844
Size: 3745 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 4498 5121 1394
Size: 429 1222 1453 623 3507
Fragments arranged by size:
3507 1453 1222 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2380 4065 4339 5729 5903 65
Size: 1183 1132 1685 274 1390 174 1396
Fragments arranged by size:
1685 1396 1390 1183 1132 274 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3450 4034 5878 6065 1700
Size: 110 1640 584 1844 187 2869
Fragments arranged by size:
2869 1844 1640 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 3089 5294 6306 3089
Size: 2205 1012 4017
Fragments arranged by size:
4017 2205 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2482 3277 794
Size: 1688 795 4751
Fragments arranged by size:
4751 1688 795
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 3857 1184
Size: 2673 4561
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4060 4365 2011
Size: 2049 305 4880
Fragments arranged by size:
4880 2049 305
BstYI r'GATC_y
Cuts at: 1972 2077 2419 2533 3303 4564 4931 5316 5333
Size: 105 342 114 770 1261 367 385 17
Cuts at: 5333 6785 6871 6883 7229 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1261 770 385 367 346 342
114 105 86 17 12
BstZ17I GTA'TAC
Cuts at: 2431 2749 2431
Size: 318 6916
BtgI C'CryG_G
Cuts at: 513 1065 3096 513
Size: 552 2031 4651
Fragments arranged by size:
4651 2031 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6495
Fragments arranged by size:
6495 683 56
BtsI GCAGTG_nn'
Cuts at: 4123 4750 5544 5564 4123
Size: 627 794 20 5793
Fragments arranged by size:
5793 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2827 2862 529
Size: 35 2263 35 4901
Fragments arranged by size:
4901 2263 35 35
DraI TTT'AAA
Cuts at: 4538 4538
Size: 7234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3237 3448 4103
Size: 54 1425 50 275 18 1407 211 655
Cuts at: 4103 4681 5570 7199 8
Size: 578 889 1629 43
Fragments arranged by size:
1629 1425 1407 889 655 578 275 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 4103 4681 1537
Size: 275 1636 655 578 4090
Fragments arranged by size:
4090 1636 655 578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2883 3488 3668 4017 5164 1353
Size: 1530 605 180 349 1147 3423
Fragments arranged by size:
3423 1530 1147 605 349 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3469 4131 5821 6195 6341 387
Size: 1064 138 1880 662 1690 374 146 1280
Fragments arranged by size:
1880 1690 1280 1064 662 374 146 138
EcoICRI GAG'CTC
Cuts at: 4516 4516
Size: 7234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2237 2299 2592 2725 3387 3417
Size: 393 544 61 62 293 133 662 30
Cuts at: 3417 3621 3668 3692 3869 3975 4271 4392 5297
Size: 204 47 24 177 106 296 121 905
Cuts at: 5297 5894 6681 6949 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 905 787 662 597 544 393 296
293 268 204 177 133 121 106 62
61 47 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2205 2545 2205
Size: 340 6894
EcoO109I rG'GnC_Cy
Cuts at: 2196 3022 3571 4171 4384 2196
Size: 826 549 600 213 5046
Fragments arranged by size:
5046 826 600 549 213
EcoRI G'AATT_C
Cuts at: 4543 4921 4543
Size: 378 6856
FspI TGC'GCA
Cuts at: 5740 5740
Size: 7234
HaeII r_GCGC'y
Cuts at: 1678 2704 3895 6381 1678
Size: 1026 1191 2486 2531
Fragments arranged by size:
2531 2486 1191 1026
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 2518 2525 2550
Size: 32 135 32 663 32 251 7 25
Cuts at: 2550 2557 3365 3397 3398 3430 5875 5907 5949
Size: 7 808 32 1 32 2445 32 42
Cuts at: 5949 5981 1373
Size: 32 2626
Fragments arranged by size:
2626 2445 808 663 251 135 42 32
32 32 32 32 32 32 25 7
7 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4578 4824 669
Size: 988 281 2640 246 3079
Fragments arranged by size:
3079 2640 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2649 2792 748
Size: 1901 143 5190
Fragments arranged by size:
5190 1901 143
HpaI GTT'AAC
Cuts at: 4824 4824
Size: 7234
KpnI G_GTAC'C
Cuts at: 2829 4559 2829
Size: 1730 5504
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 7110 7110
Size: 7234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3820 6348 6532 1305
Size: 2515 2528 184 2007
Fragments arranged by size:
2528 2515 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3239 10
Size: 54 1425 343 1407 4005
Fragments arranged by size:
4005 1425 1407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 4287 5192 5551 5710 518
Size: 648 2127 994 905 359 159 2042
Fragments arranged by size:
2127 2042 994 905 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 3365 3633 3704 3737 4650 5327 6475
Size: 651 1108 268 71 33 913 677 1148
Cuts at: 6475 6720 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1108 913 677 651 268 245
71 33
NaeI GCC'GGC
Cuts at: 3452 3452
Size: 7234
NcoI C'CATG_G
Cuts at: 513 1065 3096 513
Size: 552 2031 4651
Fragments arranged by size:
4651 2031 552
NdeI CA'TA_TG
Cuts at: 387 4183 387
Size: 3796 3438
NgoMIV G'CCGG_C
Cuts at: 3450 3450
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 2068 1052
Size: 1016 6218
NotI GC'GGCC_GC
Cuts at: 4681 4681
Size: 7234
NsiI A_TGCA'T
Cuts at: 2820 2820
Size: 7234
NspI r_CATG'y
Cuts at: 1132 1229 2597 4592 6137 1132
Size: 97 1368 1995 1545 2229
Fragments arranged by size:
2229 1995 1545 1368 97
PasI CC'CwG_GG
Cuts at: 1348 4082 1348
Size: 2734 4500
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7234
PfoI T'CCnGG_A
Cuts at: 2192 2192
Size: 7234
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 6816 6848 5303
Size: 32 1481 32 5689
Fragments arranged by size:
5689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2196 3022 2196
Size: 826 6408
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7234
PsiI TTA'TAA
Cuts at: 4804 4804
Size: 7234
PstI C_TGCA'G
Cuts at: 830 4427 4586 830
Size: 3597 159 3478
Fragments arranged by size:
3597 3478 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 3365 3704 3737 3365
Size: 339 33 6862
Fragments arranged by size:
6862 339 33
SacI G_AGCT'C
Cuts at: 4518 4518
Size: 7234
SalI G'TCGA_C
Cuts at: 1936 4576 1936
Size: 2640 4594
SapI GCTCTTCn'nnn_
Cuts at: 3668 4017 3668
Size: 349 6885
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 5482 7194 5482
Size: 1712 5522
SfcI C'TryA_G
Cuts at: 826 1045 4327 4339 4423 4582 5717 6398 6589
Size: 219 3282 12 84 159 1135 681 191
Cuts at: 6589 826
Size: 1471
Fragments arranged by size:
3282 1471 1135 681 219 191 159 84
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7234
SmaI CCC'GGG
Cuts at: 1872 4561 1872
Size: 2689 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2800 3554 5338 6239 6501 820
Size: 197 926 857 754 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 857 754 262 197
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 2597 4592 1132
Size: 1465 1995 3774
Fragments arranged by size:
3774 1995 1465
SspI AAT'ATT
Cuts at: 5 52 5158 5
Size: 47 5106 2081
Fragments arranged by size:
5106 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7234
StyI C'CwwG_G
Cuts at: 513 1065 2493 2605 3096 3117 3540 4673 513
Size: 552 1428 112 491 21 423 1133 3074
Fragments arranged by size:
3074 1428 1133 552 491 423 112 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2512 5261 5278 5431 5616 6061 325
Size: 1255 932 2749 17 153 185 445 1498
Fragments arranged by size:
2749 1498 1255 932 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5480 7192 96
Size: 274 80 33 51 1443 3503 1712 138
Fragments arranged by size:
3503 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 7194 7226 2297
Size: 32 4865 32 2305
Fragments arranged by size:
4865 2305 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3227 1316
Size: 1911 5323
XbaI T'CTAG_A
Cuts at: 4570 4570
Size: 7234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2938 4271 2938
Size: 1333 5901
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7234
XmaI C'CCGG_G
Cuts at: 1870 4559 1870
Size: 2689 4545
XmnI GAAnn'nnTTC
Cuts at: 2892 5363 2892
Size: 2471 4763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5042 276
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI