Restriction map for
pFN21AE2278
(Circular) (Six-base) MAPSORT of: pFN21AE2278.seq Check: 6618 from: 1 to: 5731
pFN21AE2278 5731 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2455 2455
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 3074 1937
Size: 124 1013 4594
Fragments arranged by size:
4594 1013 124
Acc65I G'GTAC_C
Cuts at: 2414 3052 2414
Size: 638 5093
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2961 3794 5178 5446 1632
Size: 1329 833 1384 268 1917
Fragments arranged by size:
1917 1384 1329 833 268
AfeI AGC'GCT
Cuts at: 2096 2096
Size: 5731
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2574 4630 5607 1203
Size: 1371 2056 977 1327
Fragments arranged by size:
2056 1371 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaI G_GGCC'C
Cuts at: 2830 2830
Size: 5731
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2904 3056 1870
Size: 73 961 152 4545
Fragments arranged by size:
4545 961 152 73
BamHI G'GATC_C
Cuts at: 2232 2398 3061 3428 2232
Size: 166 663 367 4535
Fragments arranged by size:
4535 663 367 166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2414 3052 4507 618
Size: 325 217 627 627 638 1455 1842
Fragments arranged by size:
1842 1455 638 627 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2830 3050 1391
Size: 1439 220 4072
Fragments arranged by size:
4072 1439 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2510 2661 928
Size: 971 611 151 3998
Fragments arranged by size:
3998 971 611 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2761 2795 3922 3956 1903
Size: 34 824 34 1127 34 3678
Fragments arranged by size:
3678 1127 824 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2723 4342 136
Size: 107 122 71 631 605 1051 1619 1525
Fragments arranged by size:
1619 1525 1051 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2031 5726 2031
Size: 3695 2036
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2830 3792 4948 1790
Size: 1040 962 1156 2573
Fragments arranged by size:
2573 1156 1040 962
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2501
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2345 2474 3035 4391 1239
Size: 856 250 129 561 1356 2579
Fragments arranged by size:
2579 1356 856 561 250 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2404 3856 4721 5019 2404
Size: 1452 865 298 3116
Fragments arranged by size:
3116 1452 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 4394 882
Size: 617 810 2085 2219
Fragments arranged by size:
2219 2085 810 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 3522 493
Size: 711 1296 1022 2702
Fragments arranged by size:
2702 1296 1022 711
BsaBI GATnn'nnATC
Cuts at: 2403 2993 5725 2403
Size: 590 2732 2409
Fragments arranged by size:
2732 2409 590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 3538 3920 4555
Size: 53 83 186 936 717 1288 382 635
Cuts at: 4555 275
Size: 1451
Fragments arranged by size:
1451 1288 936 717 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2290 2320 1178
Size: 30 165 30 887 30 4589
Fragments arranged by size:
4589 887 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2180 1186
Size: 955 39 4737
Fragments arranged by size:
4737 955 39
BseYI C'CCAG_C
Cuts at: 2110 2508 4934 5430 2110
Size: 398 2426 496 2411
Fragments arranged by size:
2426 2411 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1997 2755 2836 2981 1163
Size: 573 261 758 81 145 3913
Fragments arranged by size:
3913 758 573 261 145 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2368 3792 3877 4948 2368
Size: 1424 85 1071 3151
Fragments arranged by size:
3151 1424 1071 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 2830 3050 3792 3877 4948 1391
Size: 399 578 462 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 462 399 220 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2455 3086 844
Size: 1611 631 3489
Fragments arranged by size:
3489 1611 631
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 4226 4400 65
Size: 1183 1569 1409 174 1396
Fragments arranged by size:
1569 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 2486 3791 4803 2486
Size: 1305 1012 3414
Fragments arranged by size:
3414 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2031 2232 2398 3061 3428 3813 3830 5282
Size: 59 201 166 663 367 385 17 1452
Cuts at: 5282 5368 5380 5726 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 663 385 367 346 201 166
86 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2721 2727 513
Size: 552 1656 6 3517
Fragments arranged by size:
3517 1656 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2081 487
Size: 683 56 855 4137
Fragments arranged by size:
4137 855 683 56
BtsI GCAGTG_nn'
Cuts at: 2672 2853 2969 3247 4041 4061 2672
Size: 181 116 278 794 20 4342
Fragments arranged by size:
4342 794 278 181 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2531 4738 809
Size: 507 1215 2207 1802
Fragments arranged by size:
2207 1802 1215 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 2715 2724
Size: 54 1425 50 275 18 664 221 9
Cuts at: 2724 3178 4067 5696 8
Size: 454 889 1629 43
Fragments arranged by size:
1629 1425 889 664 454 275 221 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 1353
Size: 2308 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2612 4318 4692 4838 387
Size: 1064 138 1023 1706 374 146 1280
Fragments arranged by size:
1706 1280 1064 1023 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2345 2474 2961 3035 3794 4391
Size: 393 463 250 129 487 74 759 597
Cuts at: 4391 5178 5446 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 759 597 487 463 393 268
250 129 74
EcoO109I rG'GnC_Cy
Cuts at: 2701 2827 2917 2701
Size: 126 90 5515
Fragments arranged by size:
5515 126 90
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2051 2098 2111 4878 1678
Size: 373 47 13 2767 2531
Fragments arranged by size:
2767 2531 373 47 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2793 2825 4372 4404 4446
Size: 32 135 32 1221 32 1547 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 1547 1221 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 2418 3056 2418
Size: 638 5093
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2496 2717 2726 10
Size: 54 1425 343 664 221 9 3015
Fragments arranged by size:
3015 1425 664 343 221 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 3689 4048 4207 518
Size: 648 1560 963 359 159 2042
Fragments arranged by size:
2042 1560 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2512 2861 2939 3147 3824 4972 5217 1606
Size: 906 349 78 208 677 1148 245 2120
Fragments arranged by size:
2120 1148 906 677 349 245 208 78
NcoI C'CATG_G
Cuts at: 513 1065 2721 2727 513
Size: 552 1656 6 3517
Fragments arranged by size:
3517 1656 552 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2439 2578 2808 3089 4634 1132
Size: 97 1210 139 230 281 1545 2229
Fragments arranged by size:
2229 1545 1210 281 230 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 2574 4630 2574
Size: 2056 3675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PmlI CAC'GTG
Cuts at: 2500 2500
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 2917 2701
Size: 216 5515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2826 2826
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2860 3083 830
Size: 2030 223 3478
Fragments arranged by size:
3478 2030 223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2512 2939 2512
Size: 427 5304
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2856 3079 4214 4895 5086 826
Size: 219 1811 223 1135 681 191 1471
Fragments arranged by size:
1811 1471 1135 681 223 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2723 2723
Size: 5731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 3835 4736 4998 820
Size: 197 926 476 1416 901 262 1553
Fragments arranged by size:
1553 1416 926 901 476 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2721 2727 3170 513
Size: 552 1656 6 443 3074
Fragments arranged by size:
3074 1656 552 443 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2220 2579 2685 3758 3775 3928 4113
Size: 1255 640 359 106 1073 17 153 185
Cuts at: 4113 4558 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1073 640 445 359 185 153
106 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 3977 5689
Size: 274 80 33 51 1443 153 1847 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1847 1712 1443 274 153 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2290 2322 2727 2759 5691 5723 2290
Size: 32 405 32 2932 32 2298
Fragments arranged by size:
2932 2298 405 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 2629 2752 3067 2629
Size: 123 315 5293
Fragments arranged by size:
5293 315 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2725 2734 2449
Size: 276 9 5446
Fragments arranged by size:
5446 276 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI