Restriction map for
pFN21AE2274
(Circular) (Six-base) MAPSORT of: pFN21AE2274.seq Check: 5780 from: 1 to: 5602
pFN21AE2274 5602 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2410
Size: 5602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaI G_GGCC'C
Cuts at: 2084 2084
Size: 5602
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2856 2927 1870
Size: 73 913 71 4545
Fragments arranged by size:
4545 913 73 71
BamHI G'GATC_C
Cuts at: 2093 2932 3299 2093
Size: 839 367 4396
Fragments arranged by size:
4396 839 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2483 2923 4378 618
Size: 325 217 627 513 183 440 1455 1842
Fragments arranged by size:
1842 1455 627 513 440 325 217 183
BanII G_rGCy'C
Cuts at: 1391 2023 2084 2921 1391
Size: 632 61 837 4072
Fragments arranged by size:
4072 837 632 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2903 928
Size: 971 1004 3627
Fragments arranged by size:
3627 1004 971
BbvCI CC'TCA_GC
Cuts at: 2255 2255
Size: 5602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2314 3494 4704 2314
Size: 1180 1210 3212
Fragments arranged by size:
3212 1210 1180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2084 3663 4819 1790
Size: 294 1579 1156 2573
Fragments arranged by size:
2573 1579 1156 294
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2603 1056
Size: 1547 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2626 2726 4262 1239
Size: 1055 332 100 1536 2579
Fragments arranged by size:
2579 1536 1055 332 100
Bpu10I CC'TnA_GC
Cuts at: 2255 2255
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 4265 882
Size: 617 687 2079 2219
Fragments arranged by size:
2219 2079 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2316 2346 1178
Size: 30 165 30 913 30 4434
Fragments arranged by size:
4434 913 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2534 2540 2876 1186
Size: 1117 231 6 336 3912
Fragments arranged by size:
3912 1117 336 231 6
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2173 2675 1163
Size: 573 390 47 502 4090
Fragments arranged by size:
4090 573 502 390 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 3052 3813 4841 1540
Size: 275 186 223 828 761 1028 2301
Fragments arranged by size:
2301 1028 828 761 275 223 186
BsiHKAI G_wGCw'C
Cuts at: 3663 3748 4819 3663
Size: 85 1071 4446
Fragments arranged by size:
4446 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2084 2921 3663 3748 4819 1391
Size: 399 233 61 837 742 85 1071 2174
Fragments arranged by size:
2174 1071 837 742 399 233 85 61
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 2344 3491 5221 2344
Size: 1147 1730 2725
Fragments arranged by size:
2725 1730 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 2957 844
Size: 1566 547 3489
Fragments arranged by size:
3489 1566 547
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2483 794
Size: 1689 3913
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 2636 1184
Size: 1452 4150
BstYI r'GATC_y
Cuts at: 1972 2093 2932 3299 3684 3701 5153 5239 5251
Size: 121 839 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 839 385 367 346 121 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2085 2327 513
Size: 552 1008 12 242 3788
Fragments arranged by size:
3788 1008 552 242 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 2412 2484 3118 3912 3932 2114
Size: 298 72 634 794 20 3784
Fragments arranged by size:
3784 794 634 298 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2736 2771 529
Size: 35 2172 35 3360
Fragments arranged by size:
3360 2172 35 35
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2200 4609 809
Size: 507 884 2409 1802
Fragments arranged by size:
2409 1802 884 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2418 3049
Size: 54 1425 50 275 18 391 197 631
Cuts at: 3049 3938 5567 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 631 391 275 197 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2221 3049 1537
Size: 275 409 828 4090
Fragments arranged by size:
4090 828 409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2626 2726 3665 4262 5049 5317
Size: 393 662 332 100 939 597 787 268
Cuts at: 5317 1239
Size: 1524
Fragments arranged by size:
1524 939 787 662 597 393 332 268
100
EcoNI CCTnn'n_nnAGG
Cuts at: 2785 2785
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2038 2081 2133 2589 2038
Size: 43 52 456 5051
Fragments arranged by size:
5051 456 52 43
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 4749 1678
Size: 3071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 2707 2739 2830
Size: 32 135 32 645 32 458 32 91
Cuts at: 2830 2862 4243 4275 4317 4349 1373
Size: 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1381 645 458 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2545 3018 3695 4843 5088 1606
Size: 939 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 939 677 473 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5050
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 2599 1052
Size: 1547 4055
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2787 1348
Size: 1439 4163
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2505 2505
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2133 2038
Size: 95 5507
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2080 2080
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2730 2954 830
Size: 1900 224 3478
Fragments arranged by size:
3478 1900 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SanDI GG'GwC_CC
Cuts at: 2038 2038
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2726 2950 4085 4766 4957 826
Size: 219 1681 224 1135 681 191 1471
Fragments arranged by size:
1681 1471 1135 681 224 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2854 1517
Size: 1337 4265
StyI C'CwwG_G
Cuts at: 513 1065 2015 2747 3041 513
Size: 552 950 732 294 3074
Fragments arranged by size:
3074 950 732 552 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 2504 3629 3646 3799 3984 4429
Size: 1255 891 33 1125 17 153 185 445
Cuts at: 4429 325
Size: 1498
Fragments arranged by size:
1498 1255 1125 891 445 185 153 33
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2312 2316 2344 2348 5562 5594 2154
Size: 32 126 4 28 4 3214 32 2162
Fragments arranged by size:
3214 2162 126 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 2796 3731 2796
Size: 935 4667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI