Restriction map for
pFN21AE2271
(Circular) (Six-base) MAPSORT of: pFN21AE2271.seq Check: 8823 from: 1 to: 6079
pFN21AE2271 6079 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 2409
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2792 4142 5526 5794 1632
Size: 758 402 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 758 402 268
AflII C'TTAA_G
Cuts at: 820 1017 2300 820
Size: 197 1283 4599
Fragments arranged by size:
4599 1283 197
AflIII A'CryG_T
Cuts at: 1203 3365 4978 5955 1203
Size: 2162 1613 977 1327
Fragments arranged by size:
2162 1613 1327 977
AgeI A'CCGG_T
Cuts at: 3351 4910 3351
Size: 1559 4520
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 3182 1166
Size: 2016 4063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 4684
AlwNI CAG_nnn'CTG
Cuts at: 2419 3115 3139 3822 5394 2419
Size: 696 24 683 1572 3104
Fragments arranged by size:
3104 1572 696 683 24
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 2977 3388 3614 3766 5875 1493
Size: 1484 411 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 411 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3127 3404 1870
Size: 73 1184 277 4545
Fragments arranged by size:
4545 1184 277 73
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 3267 3273 3282 3400
Size: 325 217 627 756 724 6 9 118
Cuts at: 3400 4855 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 756 724 627 325 217 118
9 6
BanII G_rGCy'C
Cuts at: 1391 2204 2441 2532 3398 1391
Size: 813 237 91 866 4072
Fragments arranged by size:
4072 866 813 237 91
BbeI G_GCGC'C
Cuts at: 3286 3286
Size: 6079
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 2510 3338 3368 928
Size: 971 417 194 828 30 3639
Fragments arranged by size:
3639 971 828 417 194 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3039 3073 4270 4304 1903
Size: 34 1102 34 1197 34 3678
Fragments arranged by size:
3678 1197 1102 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2101 3971 5181 2101
Size: 1870 1210 2999
Fragments arranged by size:
2999 1870 1210
BclI T'GATC_A
Cuts at: 1548 2286 1548
Size: 738 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3023 3496 3023
Size: 473 5606
Bme1580I G_kGCm'C
Cuts at: 1790 3347 4140 5296 1790
Size: 1557 793 1156 2573
Fragments arranged by size:
2573 1557 1156 793
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2612 1056
Size: 1556 4523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2979 2979
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2546 2611 2804 2954 3056 4739 1239
Size: 1277 30 65 193 150 102 1683 2579
Fragments arranged by size:
2579 1683 1277 193 150 102 65 30
Bpu10I CC'TnA_GC
Cuts at: 2814 3012 2814
Size: 198 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2233 4204 5069 5367 2233
Size: 1971 865 298 2945
Fragments arranged by size:
2945 1971 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3061 4742 882
Size: 617 1562 1681 2219
Fragments arranged by size:
2219 1681 1562 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3216 3283 3886 4268
Size: 53 83 186 936 1683 67 603 382
Cuts at: 4268 4903 275
Size: 635 1451
Fragments arranged by size:
1683 1451 936 635 603 382 186 83
67 53
BsaWI w'CCGG_w
Cuts at: 3351 4512 4910 5184 5331 5742 3351
Size: 1161 398 274 147 411 3688
Fragments arranged by size:
3688 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2040 2070 1178
Size: 30 165 30 637 30 5187
Fragments arranged by size:
5187 637 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2366 2734 3308 1186
Size: 862 318 368 574 3957
Fragments arranged by size:
3957 862 574 368 318
BseYI C'CCAG_C
Cuts at: 2062 2092 3208 5282 5778 2062
Size: 30 1116 2074 496 2363
Fragments arranged by size:
2363 2074 1116 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3206 1163
Size: 573 1470 4036
Fragments arranged by size:
4036 1470 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2532 4140 4225 5296 2532
Size: 1608 85 1071 3315
Fragments arranged by size:
3315 1608 1071 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2441 2532 3347 3398 4140 4225
Size: 399 414 237 91 815 51 742 85
Cuts at: 4225 5296 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 815 742 414 399 237 91
85 51
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 3344 3434 844
Size: 1565 935 90 3489
Fragments arranged by size:
3489 1565 935 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3295 3966 1394
Size: 429 1019 453 671 3507
Fragments arranged by size:
3507 1019 671 453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2471 4574 4748 65
Size: 1183 1223 2103 174 1396
Fragments arranged by size:
2103 1396 1223 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3221 3270 3351 4723 4910 1700
Size: 110 1411 49 81 1372 187 2869
Fragments arranged by size:
2869 1411 1372 187 110 81 49
BsrGI T'GTAC_A
Cuts at: 96 2126 96
Size: 2030 4049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3115 794
Size: 2321 3758
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 2800 1184
Size: 1616 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 2540 2540
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074
Size: 1437 367 385 17 1452 86 12 346
Cuts at: 6074 1972
Size: 1977
Fragments arranged by size:
1977 1452 1437 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3093 3093
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5527
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 3116 3595 4389 4409 2713
Size: 403 479 794 20 4383
Fragments arranged by size:
4383 794 479 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3526 4415 6044
Size: 54 1425 50 275 18 1696 889 1629
Cuts at: 6044 8
Size: 43
Fragments arranged by size:
1696 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 4009 1353
Size: 785 1871 3423
Fragments arranged by size:
3423 1871 785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2744 4666 5040 5186 387
Size: 1064 138 1155 1922 374 146 1280
Fragments arranged by size:
1922 1280 1155 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2530 2530
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2516 2546 2611 2792 2804 2954
Size: 393 758 126 30 65 181 12 150
Cuts at: 2954 3056 4142 4739 5526 5794 1239
Size: 102 1086 597 787 268 1524
Fragments arranged by size:
1524 1086 787 758 597 393 268 181
150 126 102 65 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2858 2405
Size: 453 5626
EcoO109I rG'GnC_Cy
Cuts at: 2809 2999 3136 2809
Size: 190 137 5752
Fragments arranged by size:
5752 190 137
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2057 3286 5226 1678
Size: 379 1229 1940 2531
Fragments arranged by size:
2531 1940 1229 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3284 3316 4720 4752 4794
Size: 32 135 32 1712 32 1404 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 1712 1404 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3669 669
Size: 988 281 1485 246 3079
Fragments arranged by size:
3079 1485 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KasI G'GCGC_C
Cuts at: 3282 3282
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 2360 3678 2360
Size: 1318 4761
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 3174 5193 5377 1305
Size: 891 978 2019 184 2007
Fragments arranged by size:
2019 2007 978 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4257
Fragments arranged by size:
4257 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 3182 4037 4396 4555 518
Size: 648 1437 579 855 359 159 2042
Fragments arranged by size:
2042 1437 855 648 579 359 159
MspA1I CmG'CkG
Cuts at: 1606 2524 3495 4172 5320 5565 1606
Size: 918 971 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 918 677 245
NaeI GCC'GGC
Cuts at: 3223 3272 3223
Size: 49 6030
NarI GG'CG_CC
Cuts at: 3283 3283
Size: 6079
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5527
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NgoMIV G'CCGG_C
Cuts at: 3221 3270 3221
Size: 49 6030
NheI G'CTAG_C
Cuts at: 1052 2608 1052
Size: 1556 4523
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 2556 3369 3437 4982 1132
Size: 97 1327 813 68 1545 2229
Fragments arranged by size:
2229 1545 1327 813 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 3365 4978 3365
Size: 1613 4466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2754 1108
Size: 1646 4433
PfoI T'CCnGG_A
Cuts at: 2532 2532
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 2999 3136 2809
Size: 190 137 5752
Fragments arranged by size:
5752 190 137
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2524 2524
Size: 6079
SacI G_AGCT'C
Cuts at: 2532 2532
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SanDI GG'GwC_CC
Cuts at: 2999 2999
Size: 6079
SapI GCTCTTCn'nnn_
Cuts at: 2138 2138
Size: 6079
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SexAI A'CCwGG_T
Cuts at: 2943 2943
Size: 6079
SfcI C'TryA_G
Cuts at: 826 1045 3427 4562 5243 5434 826
Size: 219 2382 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3284 3284
Size: 6079
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2248 2300 4183 5084 5346 820
Size: 197 926 305 52 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 305 262 197 52
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 2864 1517
Size: 1107 240 4732
Fragments arranged by size:
4732 1107 240
StyI C'CwwG_G
Cuts at: 513 1065 2269 2430 2477 3518 513
Size: 552 1204 161 47 1041 3074
Fragments arranged by size:
3074 1204 1041 552 161 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2411 3070 4106 4123 4276 4461 4906
Size: 1255 831 659 1036 17 153 185 445
Cuts at: 4906 325
Size: 1498
Fragments arranged by size:
1498 1255 1036 831 659 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2126 4325 6037
Size: 274 80 33 51 1443 149 2199 1712
Cuts at: 6037 96
Size: 138
Fragments arranged by size:
2199 1712 1443 274 149 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 6039 6071 2070
Size: 32 3937 32 2078
Fragments arranged by size:
3937 2078 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SfiI SrfI SwaI XcmI